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Preparation method of hippocampus polypeptide enzyme solution

The technology of polypeptide enzymolysate and hippocampus is applied in the field of preparation of hippocampal polypeptide enzymolysate, which can solve the problems of no disclosure of the preparation method of hippocampus polypeptide enzymolysate, inability to fully utilize nutrients, waste of resources, etc. Small molecular weight, the effect of avoiding residues and contamination

Inactive Publication Date: 2013-09-18
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Seahorse or sea dragon medicinal wine, sea horse or sea dragon dry goods cannot make full use of the nutrients of each part of sea horse or sea dragon, which will cause some waste of resources
At present, there are no relevant research reports on the preparation method of hippocampal polypeptide hydrolyzate at home and abroad.

Method used

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  • Preparation method of hippocampus polypeptide enzyme solution
  • Preparation method of hippocampus polypeptide enzyme solution
  • Preparation method of hippocampus polypeptide enzyme solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method of hippocampal polypeptide enzymatic hydrolyzate, comprising the following steps:

[0029] (1) Degreasing of hippocampal bone powder: Freeze-dry the raw material of hippocampus at -40°C for 55 hours, crush it with a drug grinder and pass it through a 60-mesh sieve, take the filtrate to obtain hippocampal bone powder, and then use CO 2 Degreasing by supercritical extraction technology to obtain defatted hippocampus bone powder;

[0030] (2) One-time enzymatic hydrolysis: Put hippocampal defatted bone powder and water into a glass container at a ratio of 50g / L, place the container in a water bath at 55°C for 10 minutes, add trypsin with 3% weight of hippocampal defatted bone powder Finally, adjust the pH to 8.8, and adjust the pH to 8.8 every 25 minutes. After 5 hours of enzymatic hydrolysis, place the glass container in a boiling water bath to inactivate the enzyme for 10 minutes;

[0031] (3) Second enzymatic hydrolysis: continue to place the deact...

Embodiment 2

[0034] With embodiment 1, its difference is:

[0035] (1) Trypsin enzymatic hydrolysis conditions: Put hippocampal defatted bone powder and water into a glass container at a ratio of 40g / L, place the container in a water bath at 40°C for 8 minutes, add 2% hippocampal defatted bone powder by weight Adjust the pH to 8.5 after trypsin, adjust the pH to 8.5 every 20 minutes, and enzymatically hydrolyze for 4 hours;

[0036] (2) Alkaline protease enzymatic hydrolysis conditions: continue to place the inactivated glass container in a water bath at 50°C for a certain period of time, add 2% alkaline protease by weight of hippocampus defatted bone powder, and adjust the pH to 8.5. Adjust the pH to 8.5 once every 20 minutes, and enzymatically hydrolyze for 6 hours.

Embodiment 3

[0038] With embodiment 1, its difference is:

[0039] (1) Trypsin enzymatic hydrolysis conditions: Put hippocampal defatted bone powder and water into a glass container at a ratio of 60g / L, place the container in a water bath at 50°C for 12 minutes, add 4% hippocampal defatted bone powder by weight Adjust the pH to 9 after trypsin, adjust the pH to 9 every 30 minutes, and enzymatically hydrolyze for 6 hours;

[0040] (2) Alkaline protease enzymatic hydrolysis conditions: continue to place the deactivated glass container in a water bath at 60°C for a certain period of time, add 4% alkaline protease by weight of hippocampus defatted bone powder, and adjust the pH to 9.5. Adjust the pH to 9 once every 30 minutes, and enzymatically hydrolyze for 7 hours.

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Abstract

The invention discloses a preparation method of a hippocampus polypeptide enzyme solution. The preparation method is characterized by comprising the steps of: a, mixing hippocampus bone powder which is degreased by virtue of a CO2 supercritical fluid extraction technology and water according to a proportion of 40-60g / L, adding the mixture into a glass vessel, arranging the glass vessel into a water bath boiler at the temperature of 40-50 DEG C, carrying out heat preservation for 8-12 minutes, adding trypsin into the mixture according to a proportion of 2-4 percent, regulating a pH value to 8.5-9, carrying out enzymatic hydrolysis for 4-6 hours, and carrying out enzyme inactivation for 10 minutes; and b, continuously arranging the glass vessel after enzyme inactivation into the water bath boiler at the temperature of 50-60 DEG C, preserving the temperature for a period of time, adding 2-4 percent of alkaline protease into the glass vessel, regulating the pH value to 8.5-9.5, carrying out enzymatic hydrolysis for 5-7 hours, carrying out enzyme inactivation for 10 minutes, carrying out centrifugation for 10 minutes under the conditions of 5000r / min, and taking a supernatant to obtain the hippocampus polypeptide enzyme solution. The preparation method has the advantages of simple technology and convenience for operation; and in addition, the prepared polypeptide is high in hydrolysis degree, smaller in polypeptide fragment molecular weight and is easily digested and absorbed by a human body.

Description

technical field [0001] The invention relates to the field of marine drug development, in particular to a preparation method of hippocampus polypeptide enzymatic hydrolyzate. Background technique [0002] The use of hippocampus as a traditional Chinese medicinal material has a long history in our country. In Liang Dynasty Tao Hongjing's "Compendium of Materia Medica", it has been recorded that hippocampus is warm in nature and sweet in taste, and returns to the liver and kidney channels. It can cure respiratory disorders, sexual dysfunction, insomnia, fatigue, sore throat, skin diseases, dystocia and other diseases." It is called "water horse". In 739 A.D., Chen Zangqi used the name of hippocampus for the first time in "Supplements to Materia Medica" and it is still used today. "Compendium of Materia Medica" records that "mainly dystocia and blood gas pain, warm water and dirty, aphrodisiac, eliminate lumps, and treat sores, swelling and pain." . Each edition of the modern ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCY02P20/54
Inventor 徐永健姜展志宿宇婷
Owner NINGBO UNIV
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