Preparation method and use of sunflower seed polypeptide

Abstract

The invention provides a preparation method of sunflower seed polypeptide. The method comprises the following steps of: dissolving sunflower seed proteins isolate in water; hydrolyzing the sunflower seed proteins isolate with two-stage protease: alkaline protease and composite acid protease, to obtain the polypeptide, wherein during the hydrolysis, ultrasonic intermittent frequency conversion is performed to assist enzymolysis; and performing microfiltration and ultrafiltration on the enzymolysis liquid to obtain the sunflower seed polypeptide. The method has the advantages of adopting different ultrasonic frequencies and processing times for different proteases, effectively improving the hydrolysis degree and polypeptide yield of the sunflower seed proteins and obviously improving the production efficiency compared with the conventional process. A product prepared by the method has multiple health care functions of eliminating free radicals, removing nitrites, blocking the synthesis of nitrosamines, inhibiting the oxidization of red blood cells, beautifying and caring skins, preventing hepatic injury and the like and can be used for preparing functional foods and cosmetics.

Description

technical field [0001] The invention relates to deep processing of sunflower seeds, in particular to a preparation method and application of sunflower seed polypeptides. Background technique [0002] Modern studies have shown that many plant polypeptides hydrolyzed by protease have functions such as anti-oxidation, scavenging free radicals or blocking nitrosation synthesis, and can be used as raw materials for food or health products. For example, patent CN101284872 (200810064296.8) discloses zein to prepare antioxidant active peptide; patent CN101228918 (200710017281.1) discloses walnut polypeptide antioxidant powder. Some small molecular polypeptides have good transdermal absorption effect. When applied to the skin, they can not only resist the oxidation of cosmetics themselves, but also remove free radicals from the human body, inhibit the degradation of human collagen and elastin, and reduce the aging process of the skin, thereby anti-wrinkle, The effect of maintaining ...

AI Technical Summary

Problems solved by technology

There are few domestic and foreign research reports on the preparation of active peptides from sunflower protein, and most of them use single-enzyme hydrolysis, which has a low degree of hydrolysis and a low yield of small molecule peptides. For example, Ren Jian and others used alkaline protease, Flavor protease or 1.398 neutral protease carried out single-enzyme hydrolysis research on sunflower protein, and the degree of hydrolysis only reached 8-12.5% ​​(Ren Jian, Zheng Xiqun. Research on the hydrolysis of sunflower protein isolate by neutral protease. China Oils, 2007, 8; Liu Huan , Ren Jian. Research on enzymatic hydrolysis conditions of defatted sunflower meal by alkaline protease. Grain and Oil Processing, 2008, 1; There are studies on the preparation of sunflower seed peptides by papain (Bai Yu. Enzymatic method. Food and Machinery, 2007, 10) or 1.398 Bacillus subtilis neutral protease (Chen Jie. Research on enzymatic modification of sunflower protein concentrate. Journal of Agricultural Machinery, 2008 , 5) The sunflower protein is hydrolyzed, and the degree of hydrolysis is only 4-15.6%

In addition, Zhang Guohua used two-step hydrolysis of alkaline protease and pepsin to prepare sunflower meal peptides (Research on the Anti-aging Effect of Sunflower Peptides on Skin. Natural Product Research and Development, 2010, 22). The hydrolysis time is too long and the energy consumption is large. Not suitable for industrial production

[0004] At present, ultrasonic-assisted technology has been used in the process of biological enzymatic protein hydrolysis, such as the method disclosed by patent CN101457247 (200810207956.3) using one or more enzymes in proteases such as trypsin, chymotrypsin and V8 protease to rapidly hydrolyze protein Before patent CN101003826 (200610097286.5) discloses adopting pepsin, trypsin, neutral protease or papain and the combined hydrolysis of these enzymes to hydrolyze the seaweed protein, the protease liquid is mixed with the seaweed solution after ultrasonic pretreatment and carried out enzymolysis, obviously shortening However, this method of continuous use of single-frequency ultrasonic-assisted enzymatic hydrolysis often causes problems such as decreased enzyme activity and damaged protein action sites.

Images