Preparation method of oligopeptide bean pulp used for aquatic feed
The technology of oligopeptide soybean meal and aquatic feed is applied in the field of preparation of oligopeptide soybean meal for aquatic feed. Effect of Peptide Yield
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Embodiment 1
[0017] Embodiment 1: (alkaline protease and flavor protease)
[0018] (1) Take 1 kg of defatted soybean meal, add 10 L of water to homogenate, and extract at 90 °C for 30 min.
[0019] (2) Adjust the pH of the homogenate in step 1 to 8.0 with 1 mol / L sodium hydroxide, and adjust the temperature of the system to 60°C.
[0020] (3) According to the enzyme activity ratio (alkaline protease: flavor protease) 2:1, the ratio of enzyme to substrate is 3000 U / g, add the mixture of alkaline protease and flavor protease.
[0021] (4) Heat preservation and hydrolysis at 60°C for 7.5 hours.
[0022] (5) Inactivate the enzyme at 90 °C for 10 min, then dry the solution at 70 °C.
[0023] (6) Grind the dried samples into powder by using a cyclone mill to obtain the finished product.
Embodiment 2
[0024] Embodiment 2: (papain and flavor protease)
[0025] (1) Take 1 kg of defatted soybean meal, add 10 L of water to homogenate, and extract at 90 °C for 30 min.
[0026] (2) Adjust the pH of the homogenate in step 1 to 7.0 with 1 mol / L sodium hydroxide, and adjust the temperature of the system to 45°C.
[0027] (3) According to the enzyme activity ratio (papain: flavor protease) 1:1, the enzyme-to-substrate ratio is 4000 U / g, add the mixture of papain and flavor protease.
[0028] (4) Heat preservation and hydrolysis at 45°C for 9 h.
[0029] (5) Inactivate the enzyme at 90 °C for 10 min, then dry the solution at 70 °C.
[0030] (6) Grind the dried samples into powder by using a cyclone mill to obtain the finished product.
[0031] Product processing quality inspection
[0032] Two kinds of oligopeptide soybean meal products were made, and their viscoelasticity was evaluated and the dissolution rate was determined.
[0033] Example 1: The product has good viscoelastic...
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