38results about How to "Increase the rate of enzymatic hydrolysis" patented technology

The invention discloses a method using Spanish mackerel to cooperatively produce cartilage polysaccharide and bone collagen active peptide. The method includes the following steps: 1) drying and crushing Spanish mackerel bones after cleaning; 2) placing bone powder under far-infrared rays to perform irradiation; 3) performing hot press extraction after mixing the bone powder and water; 4) using compound proteinase to perform enzymolysis; and 5) performing ultrafiltration and separation, and respectively obtaining cartilage polysaccharide and bone collagen active peptide. The method uses the far-infrared rays to make organism cells being at the highest vibration energy level, thus, biomacromolecule in the bone powder can be extracted and decomposed more easily during the follow-up hot pressextraction and enzymolysis, so that the cartilage polysaccharide and bone collagen active peptide with higher additional values can be decomposed and generated while the speed of enzymolysis processes are greatly accelerated.

The invention discloses a method for co-producing a bovine bone essence and a bone collagen peptide by using bovine bones. The method comprises the following steps: 1) cleaning the bovine bones, and carrying out crushing; 2) irradiating bone meal under far infrared rays; 3) mixing the bone meal with water, and performing hot-pressing extraction; 4) carrying out enzymolysis by using compound protease and flavourzyme respectively; 5) carrying out ultrafiltration and separation so as to respectively obtain a Meidela reaction precursor and an ossein active peptide precursor; and 6) respectively carrying out Meidela reaction and post-treatment so as to obtain the bovine bone essence and the bone collagen bioactive peptide. According to the method, by using of the far infrared rays, biological cells are at the highest vibration energy level, so biological macromolecules in the bone meal are extracted and decomposed more easily in the subsequent hot-pressing extraction and enzymolysis processes; the enzymolysis process rate is greatly increased; meanwhile, the bovine bone essence and the bone collagen active peptide with higher nutritional values can be decomposed and generated.

The invention relates to a low salt fish sauce quick brewing method by using waste water from fish protein powder processing as a raw material. The method uses fish protein powder and waste water from minced fillet production as raw materials, and employs exogenous alkali protease and flavor protease to hydrolyze protein resources in the waste water from fish protein powder processing, so as to prepare quick brewed low salt fish sauce. The fish sauce prepared by the method provided by the invention contains total nitrogen and amino acid nitrogen respectively reaching 2.2-2.4 g / 100mL and 1.1-1.3 g / 100mL, low content of volatile basic nitrogen, reaches the level of first grade fish sauce, and also has the flavor of fish sauce produced by traditional fermentation. Compared with the prior art, the invention uses wastewater from pressing of marine low value fish in fish protein powder production, and employs an exogenous biological enzymatic hydrolysis method, which not only effectively improves enzymolysis rate and product flavor and lowers salinity, but also greatly reduces the production cost, production cycle and environmental pollution.

The present invention provides a method of in-situ enzymatic hydrolysis of cellulose, 1-hydroxyalkyl and 3-methyl imidazolium carboxylated ionic liquid can be taken as a reaction medium, the method comprises the following steps: putting microcrystalline cellulose in the ionic liquid at the temperature of 70 DEG C-80 DEG C to obtain a homogeneous solution, wherein the weight of cellulose accounts for 2-6% of that of ionic liquid; adding a citric acid buffer with pH value of 4.8 and cellulase at the temperature of 50-60 DEG C, wherein the addition amount of citric acid buffer accounts for 5-10% of weight of ionic liquid, cellulase is added in an amount of 1-3mg per ml of ionic liquid, and reacting for 30-60 minutes; after enzymatic hydrolysis, separating the ionic liquid and reducing sugar through an absorption resin column, wherein the recovered ionic liquid enables multiple reusage. The method of the invention is simple, compared with a traditional cellulose enzymatic hydrolysis and a current two-step method of cellulase pretreatment by ionic liquid and regenerated enzymatic hydrolysis, the present invention has the advantages of high efficiency and environmental friendly characteristic.

The invention relates to the technical field of food processing, and discloses a method for extracting collagen in a fish skin by low-dose irradiation. Main processes comprise the following steps of making the fish skin into fish-skin minced meat, soaking the fish-skin minced meat in a fishiness removing solution, then making the fish-skin minced meat into fish-skin material liquid, afterwards, adding glyceride hydrolase, a flavor enzyme and a compound catabolic enzyme into the fish-skin material liquid, putting an obtained mixture in a cobalt-60 irradiation field to carry out irradiation, making the irradiated liquid material into a concentrated solution through heating inactivation, filtration, discoloration and concentration, and subjecting the concentrated solution to spray drying to make the collagen. According to the method for extracting the collagen by the low-dose irradiation, which is provided by the invention, through applying the irradiation to the material liquid in enzymolysis and degreasing processes, the enzymolysis rate can be improved; the hydrolyzation time is decreased; the degreasing effect is intensified; the yield and the quality of the collagen are improved; moreover, a microorganism can be effectively killed; the corrosion and deterioration prevention effects of the collagen are promoted; the preservation capacity of the collagen is enhanced; further, the molecular chain of the collagen becomes short; the average molecular weight is 1,500 to 3,500 daltons; the collagen is more suitably absorbed by a human body.