38results about How to "Increase the rate of enzymatic hydrolysis" patented technology

The invention discloses a method for preparing ultra-low molecular weight hyaluronic acid oligosaccharides and a salt thereof through combination of solid-liquid biphasic enzymolysis and ultrafiltration. The method comprises the following steps: degrading hyaluronic acid and a salt thereof by utilizing hyaluronidase produced by bacilli; preparing high-concentration hyaluronic acid enzymatic hydrolysate by adopting the method of combining a solid-liquid biphasic enzymolysis system and an ultrafiltration system, and then performing inactivating, activated carbon adsorption and impurity removal,filtering and spray-drying to obtain the ultra-low molecular weight hyaluronic acid oligosaccharides and the salt thereof with the molecular weight of less than or equal to 3 kDa. The product preparedby the method is high in stability, ultra-low in molecular weight, good in inflammation resistance and percutaneous absorptivity, high in purity, strong in oxidation resistance, does not have cytotoxicity, has the advantages of repairing skin cell damage and the like, and can be widely applied to the fields of cosmetics, food and medicines. According to the method, the process flow is easy to operate; conditions are mild; various alcohols are not used; the energy consumption is relatively low; the method does not have damage to a product structure, does not have environmental pollution, and is suitable for large-scale industrial production.

The invention belongs to the field of nonchemical modified starch and relates to a method for producing digestion starch, in particular to a method for preparing digestion starch containing slow digestion starch by enzymolysis. In order to solve the problem that the content of the slow digestion starch in the digestion starch is difficult to further improve due to the fact that the prior art for preparing the digestion starch is limited by the proportion of alpha-1, 6 glucosidic bond, the invention provides the method for preparing digestion starch containing slow digestion starch by enzymolysis. The method effectively solves such the technical problem by adding transglucosylase and amylase to the enzymolysis process of starch paste prepared from starch.

The invention relates to a method for preparing resistant dextrin by using microwaves. The method comprises the steps as follows: (1) adding an acid solution to dry starch powder, and uniformly stirring the mixture to prepare a starch acid treated sample; (2) treating the starch acid treated sample in a microwave device to prepare a resistant dextrin crude product; and (3) dissolving the resistantdextrin crude product in water, carrying out enzymolysis by alpha-high-temperature-resistant amylase and complex glucoamylase, and then, performing decolorization, centrifugation, nanofiltration andspray drying to prepare the resistant dextrin. The resistant dextrin is prepared by the microwave method, the problem that products pretreated by a traditional acid heat method are not uniformly heated is solved, and the product quality is improved. Meanwhile, a particle carbon pile decolorization method is adopted in a resistant dextrin decolorization process, and the defects that powdery activated carbon is disposable, materials remaining in waste carbon after decolorization cannot be recovered, the material loss is larger, the yield is reduced, the labor intensity is high and the like are overcome. The decolorization method can reduce energy consumption in the production process, so that the production cost is saved.

The invention relates to the field of polypeptides, in particular to a preparation method of a sea cucumber antioxidant chelating peptide with high heat stability. A complex formed by sea cucumber protein and polysaccharide is sequentially subjected to hydrolase enzymolysis and metal ion chelation to obtain the sea cucumber antioxidant chelating peptide. The complex is formed through a Maillard reaction between the sea cucumber protein and polysaccharide. The sea cucumber protein and polysaccharide are subjected to the Maillard reaction under supercritical CO2. The reaction conditions are thatthe pressure is 40-60 MPa, the temperature is 140-160 DEG C, and the reaction time is 15-25 min. The sea cucumber antioxidant chelating peptide prepared by means of the method has high heat stabilityand high antioxidant activity.

The invention discloses a method for making a dried orange peel beverage through composite enzymolysis of dried orange peel. The method comprises the specific steps of removing astringent taste, performing extraction, performing enzyme deactivation, performing blending, performing filtration, performing sterilization and performing filling. The dried orange peel beverage prepared by the method disclosed by the invention is good in mouth feel and has peculiar flavor of the dried orange peel; and besides, the content of flavedo glycoside in the beverage is high, and the extraction rate of the flavedo glycoside can be as high as 3.17%. The method disclosed by the invention is simple in technology, is free from residuals of organic reagents, does not cause pollution to environment, and is good in production safety performance and suitable for industrial production.

The invention provides an extraction method of small molecular rhizoma polygonati polypeptide. The extraction method comprises the following steps: step 1, slicing rhizoma polygonati and adding purified water; uniformly stirring and decocting to obtain a decocted mixture; step 2, cooling the decocted mixture to 55 to 58 DEG C; adding cellulase, amylase and a saccharifying enzyme, and stirring andcarrying out enzymolysis to obtain a first enzymolysis solution; step 3, centrifuging the first enzymolysis solution and separating to obtain desugared rhizoma polygonati slices; adding purified waterinto the desugared rhizoma polygonati slices; raising the temperature to 50 to 55 DEG C and adding exoaspergillus oryzae neutral protease, fresh ginger protease and kiwi fruit protease; stirring andcarrying out enzymolysis to obtain a second enzymolysis solution; step 4, heating the second enzymolysis solution and raising the temperature to carry out enzyme inactivation; separating and purifyingto obtain the small molecular rhizoma polygonati polypeptide. The extraction method provided by the invention adopts a two-time enzymolysis method of a compound enzyme, and a centrifuging technologyand a nano-filtering technology are combined, so that a prepared rhizoma polygonati peptide product has high purity and the content of the peptide with a peptide molecular weight of 180 to 500 Daltonsreaches 99 percent or above.

Provided are a composite highland flour rich in slow-digesting starch and a preparation method thereof. The flour is prepared by enzymatic hydrolysis of brown agaricus bisporus and highland flour as raw materials, and the flour is prepared from, by weight, 30-56% of the slow-digesting starch, 10-40% of fast-digesting starch and 10-40% of digesting-resistant starch. The composite highland flour isrich in the slow-digesting starch, the slow-digesting starch has a high content of beta-glucan, which can be slowly digested, and a food prepared by using a composite highland flour product exists a long time in a stomach and intestines, promotes full movements of the intestines, can maintain satiety for a long period of time, and has sustained release of energy, low glycemic index and high glucose utilization.

The invention discloses a method for extracting collagen polypeptide from fish scale. The method comprises the following steps: pretreatment, extraction, hydrolysis, metal removal, and purification; wherein a hydrolysis step is characterized by comprising the following steps: fish scale collagen and subtilisin are added to distilled water and the materials are completely dissolved, t-butylhydroperoxide and stevioside are added, the pH value is adjusted, and double-frequency ultrasonic assisted enzymatic hydrolysis is carried out on an enzymatic hydrolysate in a constant temperature water bath;a trace amount of t-butylhydroperoxide and stevioside generate synergism, which can increase the degree of hydrolysis of collagen and increase the enzymatic hydrolysis rate of subtilisin; wherein a metal removal step is characterized by comprising the following steps: starch xanthate and mulberry polysaccharide are added to the enzymatic hydrolysate, and the steps of mixing, reacting, and filtering through diatomite are carried out to obtain fish scale collagen polypeptide clear liquid; and the starch xanthate and mulberry polysaccharide have higher removal rates of heavy metal ions. The method has the beneficial effects that the method for extracting the collagen polypeptide from fish scale provided by the invention has the advantages of easy control, complete hydrolysis, large proportion of polypeptide in the product, easy absorption by the body, and low content of heavy metals.

The invention relates to the technical field of cell isolated culture, particularly a digestive enzyme composition and application thereof, and an isolated culture method of umbilical epithelial cells. The digestive enzyme composition comprises hyaluronidase, collagenase VIII and pancreatin. The umbilical epithelial cell primary isolation adopts the hyaluronidase, collagenase VIII and pancreatin for combined digestion. The digestive enzyme composition has the advantages of mild properties, low cell trauma and high enzymolysis rate and greatly enhances the epithelial cell isolation efficiency.

The present invention discloses a heparin sodium crude product refining optimization technology characterized in that: the heparin sodium crude product refining optimization technology can be completed after crushing screening, dissolution, enzymolysis, filtration, oxidation, precipitation, and drying for dehydration. By control of oxidizing agent hydrogen peroxide addition amount and number of oxidation times, heparin sodium can be refined by combination of methods of adsorption for impurity removal and enzymolysis, impurities in the heparin sodium can be more thoroughly removed, by adding of an enzyme activator, enzymolysis rate is greatly improved, the enzyme use amount is reduced, production cycle is shortened, production cost is reduced, and industrial production is facilitated.

The invention provides a sargassum polysaccharide food packaging film and a preparation method thereof, and belongs to the technical field of food packaging films. According to the food packaging filmprovided by the invention, sargassum is taken as a raw material, and sargassum polysaccharide is extracted in a combination mode of ultrasonic and enzymolysis, so that the extraction rate of polysaccharide is increased, and extraction efficiency is improved. The sargassum polysaccharide has good water solubility, has high viscosity and solidification capability and is good in film-forming property. Hydrophobic agent glycerol and plasticizer gelatin are added into the formula, so that hydrophilicity of the polysaccharide is reduced, and mechanical properties of the film are improved. The obtained food packaging film has various effects of resisting oxidation, reducing blood fat, resisting radiation, being safe and harmless, being degradable and the like.

The invention discloses a separation, extraction and purification method for cornus officinalis polysaccharides. The method includes the following steps that a) dry cornus officinalis materials are smashed into cornus-officinalis ultrafine powder materials; b) the cornus-officinalis ultrafine powder materials are subjected to ultrasonic and enzymolysis in a cultivating tank, distilled water is added, and cornus-officinalis-polysaccharide extracting liquid is obtained; c) the cornus-officinalis-polysaccharide extracting liquid is subjected to enzyme killing treatment; d) ultrasonic, filtration,standing by adding ethyl alcohol, suction filtration and drying are carried out, and a cornus-officinalis-polysaccharide crude product is obtained; e) the cornus-officinalis-polysaccharide crude product is subjected to protein removing in a classified mode; f) the obtained product is dissolved into distilled water, centrifugal separation is carried out, supernate is taken and subjected to a DEAE-cellulose column and a chromatographic column; g) vacuum low-temperature sublimation is carried out with an eluant, and white cornus officinalis polysaccharides are obtained. According to the separation, extraction and purification method, enzymolysis, protein removing in a classified mode and distilled water chromatography are carried out in the compound enzyme spray environment, extracting timeis short, the extracting efficiency is high, the product purity of the cornus officinalis polysaccharides is high, and the industrial and experimental requirements are met.

The invention discloses a sugarcane tip rot pathogenic bacterium protoplast preparation and regeneration method which specifically comprises the following steps: (1) preparing a sugarcane tip rot pathogenic bacterium spore suspension; (2) preparing fresh mycelia; (3) preparing lyticase; (4) carrying out enzymolysis of hypha cell walls; (5) collecting protoplast; (6) regenerating the protoplast; (7) investigating regeneration situations. The method is low in cost, simple to operate, low in equipment requirement, short in enzymolysis time and regeneration cycle, capable of greatly improving sugarcane tip rot pathogenic bacterium protoplast preparation efficiency and well maintaining activity of the protoplast, good in regeneration capability and beneficial to genetic transformation of laterpathogenic bacteria and screening of transformants.

The invention provides a Maiwei Dihuang Wan traditional Chinese medicine residue biological fermentation product and a preparation method and application thereof. A biological fermentation product isprepared by that Maiwei Dihuang Wan traditional Chinese medicine residues are used as raw materials, and lactobacillus plantarum (Lp) and enzymes are added for solid-state fermentation, wherein the enzyme is selected from xylanase(Xy), trichoderma reesei (Tr) and Huafen enzymes (He). Experiments find that Lp+Xy is adopted for solid-state fermentation, so that the effect for increasing the nutrientvalue of traditional Chinese medicine residue products is obvious, the content of crude fibers is 41.13%, the content of hemicellulose is 6.82%, the content of lignin is 14.44%, the content of ash is10.76%, the content of crude protein is 11.83%, the content of total sugar is 7.10%, the ammonia nitrogen value is 0.80%, ethanol is 16%, lactic acid is 9.5%, and pH is 3.36. A compound additive is used for solid-state fermentation on wheat-flavor traditional Chinese medicine residues, so that the nutrient value can be increased. The wheat-flavor traditional Chinese medicine residue biological fermentation products are used as an animal feed to replace a concentrated feed, so that the growing performance of cattle can be improved.

The invention relates to Singapore-laksa-flavor paste. The Singapore-laksa-flavor paste comprises the following ingredients in parts by weight: 60-80 parts of an enzymatic hydrolyzate of seafoods withcoconut juice, 2-5 parts of a yeast extract, 5-10 parts of homemade spices, 5-10 parts of edible salt, 5-10 parts of white granulated sugar, and 5-10 parts of corn starch. The Singapore-laksa-flavorpaste disclosed by the invention is refined by adopting modern processing technologies, including a directed bioenzymatic bydrolysis technology, a mild Maillard reaction technology, a simulated kitchen conditioning technology and the like; and thus, the Singapore-laksa-flavor paste is obvious in Singapore laksa flavor characteristics as well as strong and spicy in taste, and perfectly integrates mellow coconut aroma and palatable taste of sweet shrimps. Therefore, the Singapore-laksa-flavor paste is sweet, salty and spicy in taste, and can be directly applied in the fields of leisure foods, puffed foods, instant foods, catering and the like so as to meet pursuit of people for delicacy.

The invention discloses an enzymolysis apparatus for embryonic egg white. The enzymolysis apparatus comprises a main box body, wherein a first water tank and a second water tank are arranged at the upper end of the main box body, an enzymolysis tank is arranged on the upper end face of the middle of a mounting bottom plate, a left temperature-control clamping plate is fixedly connected with the upper ends of a set of sliding blocks, and a right temperature-control clamping plate is fixedly connected with the upper ends of the other set of sliding blocks. According to the enzymolysis apparatusand method for the embryonic egg white, the left temperature-control clamping plate and the right temperature-control clamping plate are used in cooperation, and therefore heating and temperature-control can be performed on the enzymolysis tank from the outside of the enzymolysis tank; and a first stirring blade, a second stirring blade, a third stirring blade and a fourth stirring blade are usedin cooperation, so that the temperature inside the enzymolysis tank can be controlled, the temperature inside the enzymolysis tank and the temperature outside the enzymolysis tank are doubly controlled, a stable and appropriate temperature can be better provided for the enzymolysis of the embryonic egg white, the enzymolysis rate is promoted, and therefore the enzymolysis apparatus has relativelygood practicability.