A method for extracting collagen from fish skin by low-dose irradiation

A collagen, low-dose technology, applied in chemical instruments and methods, animal/human protein, fermentation, etc., can solve the problems of long preservation time, high collagen yield, low collagen yield and quality, and achieve extended Fresh-keeping time, increase enzymatic hydrolysis rate, prolong the effect of fresh-keeping effect

Active Publication Date: 2020-06-09
舟山出入境检验检疫局综合技术服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of low collagen yield and low quality in the prior art of extracting collagen from fish skin, the purpose of the present invention is to provide a low-dose irradiated extract from fish skin that can effectively improve the yield and quality of collagen. Collagen method, the yield of collagen is high, and the fresh-keeping time is long, and the fresh-keeping effect is good

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for extracting collagen in fish skin by low-dose irradiation, comprising the following steps:

[0028] (1) Take fresh fish skin or fish skin leftovers after processing fish meat, wash it with water, and then grind it to make fish skin and meat;

[0029] (2) Soak the minced fish skin and meat in the deodorizing solution at 10 ℃ for 4 hours, rinse and dry to make fish skin dry powder. The deodorizing solution is preferably prepared by the following process: 10 g of astragalus, 8 g of Boil 4.5 g of ginger and 6 g of fennel seeds in 400 g of water, then filter, add 50 g of edible vinegar, 50 g of rice wine, and 12 g of honey to the filtrate and stir evenly;

[0030] (3) adding water to the dry fish skin powder and adjusting the pH value of the solution to 5.5 to prepare a fish skin material solution with a mass concentration of 45%;

[0031] (4) Transfer the fish skin material liquid into a transparent glass, add glyceride hydrolase and flavor enzyme, and then pla...

Embodiment 2

[0038] A method for extracting collagen in fish skin by low-dose irradiation, comprising the following steps:

[0039] (1) Take fresh fish skin or fish skin leftovers after processing fish meat, wash it with water, and then grind it to make fish skin and meat;

[0040] (2) Soak the minced fish skin and meat in the deodorizing solution at 20 °C for 5 hours, rinse and dry to make fish skin dry powder. The deodorizing solution is preferably prepared by the following process: 12.5 g of astragalus, 10 g of Boil 5 g of ginger and 7 g of fennel seeds in 450 g of water, then filter, add 65 g of edible vinegar, 65 g of rice wine, and 14 g of honey to the filtrate and stir evenly;

[0041] (3) adding water to the dry fish skin powder and adjusting the pH value of the solution to 6.5 to prepare a fish skin material solution with a mass concentration of 50%;

[0042] (4) Transfer the fish skin material liquid into a transparent glass, add glyceride hydrolase and flavor enzyme, and then pla...

Embodiment 3

[0049] A method for extracting collagen in fish skin by low-dose irradiation, comprising the following steps:

[0050] (1) Take fresh fish skin or fish skin leftovers after processing fish meat, wash it with water, and then grind it to make fish skin and meat;

[0051] (2) Soak the minced fish skin and meat in the deodorizing solution at 30 °C for 6 hours, rinse and dry to make fish skin dry powder. The deodorizing solution is preferably prepared by the following process: 15 g of astragalus, 12 g of Boil 5.5 g of ginger and 8 g of fennel seeds in 500 g of water, then filter, add 80 g of edible vinegar, 80 g of rice wine, and 16 g of honey to the filtrate and stir evenly;

[0052] (3) adding water to the dry fish skin powder and adjusting the pH value of the solution to 7.5 to prepare a fish skin material solution with a mass concentration of 55%;

[0053] (4) Transfer the fish skin material liquid into a transparent glass, add glyceride hydrolase and flavor enzyme, and then p...

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Abstract

The invention relates to the technical field of food processing, and discloses a method for extracting collagen in a fish skin by low-dose irradiation. Main processes comprise the following steps of making the fish skin into fish-skin minced meat, soaking the fish-skin minced meat in a fishiness removing solution, then making the fish-skin minced meat into fish-skin material liquid, afterwards, adding glyceride hydrolase, a flavor enzyme and a compound catabolic enzyme into the fish-skin material liquid, putting an obtained mixture in a cobalt-60 irradiation field to carry out irradiation, making the irradiated liquid material into a concentrated solution through heating inactivation, filtration, discoloration and concentration, and subjecting the concentrated solution to spray drying to make the collagen. According to the method for extracting the collagen by the low-dose irradiation, which is provided by the invention, through applying the irradiation to the material liquid in enzymolysis and degreasing processes, the enzymolysis rate can be improved; the hydrolyzation time is decreased; the degreasing effect is intensified; the yield and the quality of the collagen are improved; moreover, a microorganism can be effectively killed; the corrosion and deterioration prevention effects of the collagen are promoted; the preservation capacity of the collagen is enhanced; further, the molecular chain of the collagen becomes short; the average molecular weight is 1,500 to 3,500 daltons; the collagen is more suitably absorbed by a human body.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a method for extracting collagen from fish skin through low-dose irradiation. Background technique [0002] Collagen is widely distributed in the skin, bone, connective tissue, tendon, cornea, visceral interstitium, muscle cavity, sclera and other parts of animals. Because of its good biocompatibility, biodegradability and biological activity, it is widely used in food, It has been widely used in medicine, tissue engineering, cosmetics and other fields. Due to the differences in amino acid composition and cross-linking degree, the abundant collagen contained in aquatic animals, especially processing waste-skin, bone and scale, has many advantages that livestock collagen does not have, such as low antigenicity and low allergy characteristics such as sex. At the same time, the collagen content in aquatic animals is also higher than that in terrestrial animals. For example...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78
CPCC07K14/78C12P21/06
Inventor 邵宏宏相兴伟周秀锦周向阳王琦傅谧妮黄银志鲁华
Owner 舟山出入境检验检疫局综合技术服务中心
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