Method for preparing digestion starch containing slow digestion starch by enzymolysis

A technology of slowly digesting starch and digesting starch, which is applied in the field of producing digested starch and enzymatically producing digested starch containing slowly digesting starch, which can solve the problem that the content of slowly digesting starch is difficult to further increase, and achieve the goal of increasing the content and increasing the rate of enzymatic hydrolysis Effect

Inactive Publication Date: 2010-06-02
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention lies in the fact that the existing process for preparing digested starch is limited by the ratio of α-1,6 glycosidic bonds, and the content...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 常规玉米淀粉(干重,80g)与水混合制备质量百分比浓度为8%的淀粉乳,并在2L烧杯中,于80℃搅拌加热10min得到淀粉糊;

[0030] 随后对淀粉糊进行121℃高压灭菌20min,冷却后加入乙酸钠缓冲溶液(360mL,0.5mol / L,pH值为5.0),然后放入55℃水浴中搅拌10min,并加水使加入乙酸钠缓冲溶液后的淀粉糊总体积达到1800mL。

[0031] 向淀粉糊加入β-淀粉酶和转葡糖苷酶,酶解时间3小时,其中β-淀粉酶加酶量为0.64%的干重淀粉量,转葡糖苷酶加酶量为0.60%的干重淀粉量;

[0032] 酶解结束后加入等体积(相对于酶解后的总体积而言)的无水乙醇,在3000rpm下离心10min,并用800mL去离子水溶解得到的沉淀物,随后先将溶解液在沸水浴中搅拌加热30min,然后再在室温下搅拌放置过夜,最后进行喷雾干燥,喷雾干燥液体流速为3mL / min,进口和出口温度分别为120℃和75℃,即得到慢消化淀粉的质量百分比含量为52%的消化淀粉,而异麦芽低聚糖的质量百分比含量为10.5%。

Embodiment 2

[0034] 常规玉米淀粉(干重,80g)与水混合制备质量百分比浓度为8%的淀粉乳,并在2L烧杯中,于80℃搅拌加热10min得到淀粉糊;

[0035] 随后对淀粉糊进行121℃高压灭菌20min,冷却后加入乙酸钠缓冲溶液(360mL,0.5mol / L,pH值为5.0),然后放入55℃水浴中搅拌10min,并加水使加入乙酸钠缓冲溶液后的淀粉糊总体积达到1800mL。

[0036] 向淀粉糊加入α-麦芽糖淀粉酶和转葡糖苷酶,酶解时间3小时,其中α-麦芽糖淀粉酶加酶量为0.70%干重淀粉量,转葡糖苷酶加酶量为0.64%的干重淀粉量,再加入氯化钙,使氯化钙最终浓度为1.78mmol / L,以维持酶的活性。

[0037] 酶解结束后加入等体积(相对于酶解后的总体积而言)无水乙醇,在3000rpm下离心10min,并用800mL去离子水溶解得到的沉淀物,随后先将溶解液在沸水浴中搅拌加热30min,然后再在室温下搅拌放置过夜,最后进行喷雾干燥,喷雾干燥液体流速为3mL / min,进口和出口温度分别为120℃和75℃,即得到慢消化淀粉质量百分比含量为54%的消化淀粉,而异麦芽低聚糖的质量百分比含量为13.6%。

Embodiment 3

[0039] Conventional potato starch (dry weight, 80g) was mixed with water to prepare starch milk with a mass percentage concentration of 8%, and in a 2L beaker, stirred and heated at 7°C for 10min to obtain a starch paste;

[0040] Then the starch paste was autoclaved at 121°C for 20min, and after cooling, sodium acetate buffer solution (360mL, 0.5mol / L, pH value 5.0) was added, then placed in a 55°C water bath and stirred for 10min, and water was added to make the sodium acetate buffer solution The total volume of the starch paste after the solution reaches 1800mL;

[0041] Add β-amylase and transglucosidase to the starch paste, enzymolysis time is 3 hours, wherein the enzyme amount of β-amylase is 0.64% of dry weight starch, and the enzyme amount of transglucosidase is 0.6% of dry weight amount of starch;

[0042] After enzymolysis, add an equal volume (relative to the total volume after enzymolysis) of absolute ethanol, centrifuge at 3000rpm for 10min, and dissolve the prec...

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Abstract

The invention belongs to the field of nonchemical modified starch and relates to a method for producing digestion starch, in particular to a method for preparing digestion starch containing slow digestion starch by enzymolysis. In order to solve the problem that the content of the slow digestion starch in the digestion starch is difficult to further improve due to the fact that the prior art for preparing the digestion starch is limited by the proportion of alpha-1, 6 glucosidic bond, the invention provides the method for preparing digestion starch containing slow digestion starch by enzymolysis. The method effectively solves such the technical problem by adding transglucosylase and amylase to the enzymolysis process of starch paste prepared from starch.

Description

technical field [0001] 本发明属于非化学改性淀粉领域,涉及一种生产消化淀粉的方法,特别涉及一种酶解生产含有慢消化淀粉的消化淀粉的方法。 Background technique [0002] 淀粉是人类膳食中主要的碳水化合物。根据体外的消化率和消化时间,淀粉可分为三类:(1)快速消化淀粉(rapidly digestible starch,RDS),在体内20min可以被迅速消化吸收的淀粉;(2)慢消化淀粉(slowly digestible starch,SDS),是指那些能在小肠中被完全消化吸收但消化速率较慢的淀粉,消化时间为20-120min;(3)耐消化淀粉(resistant starch,RS),指在人体小肠内无法消化吸收的淀粉,只能在大肠中被微生物发酵利用。 [0003] 有文献报道血糖指数与快消化淀粉有很大的正相关性。血糖指数(GI)被定义为食用含一定量价值的碳水化合物的食物后,在一定时间内体内血糖水平应答与食用相当量的葡萄糖或白面包引起的血糖应答水平的比值。血糖指数与人体的糖尿病、前驱糖尿病、心血管疾病和肥胖病等生理疾病有关。高血糖指数膳食能引起老鼠血管壁的脂肪沉积,脂肪含量是摄入低血糖指数膳食老鼠脂肪含量的2倍。对于肥胖的青少年,从高血糖指数膳食中摄入的快速吸收的葡萄糖,能引起一系列荷尔蒙和新陈代谢的失调,这些变化都是与过多的食物摄入有关。低血糖指数的膳食降低老鼠的非空腹的血浆葡萄糖、血浆甘油三酯和脂肪细胞的体积,延长肥胖人群的饱腹感。慢消化淀粉具有特殊的生理学功能,如缓慢吸收、持续释放能量、有助于维持血糖稳态,预防和治疗各种疾病(如糖尿病、糖原贮积病、心脑血管疾病等)。因而,缓慢消化淀粉可应用于开发特定保健食品、热量持续型运动饮料等产品,还可以作为药物治疗糖尿病、心脑血管疾病、肥胖等。 [0004] 缓慢消化淀粉由大部分无定形成分和小部分有序结构的双螺旋成分构成。目前的慢消化淀粉生产方法是酶脱支后重结晶,一般是经过普鲁兰酶脱支处理后产生更多的短链,他们通过氢键与疏水作用进行排列和交连,聚集形成结晶结构即产生缓慢消化淀粉,但酶解脱支结晶的效果会受到淀粉分支程度即α-1,6糖苷键的比例的限制。 Contents of the invention [0005] 本发明所要解决的技术问题...

Claims

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Application Information

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IPC IPC(8): A23L1/09A23L29/30
Inventor 唐雪明王金斌谭芙蓉朱宏蒋玲曦
Owner SHANGHAI ACAD OF AGRI SCI
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