Extraction method of small molecular rhizoma polygonati polypeptide

An extraction method and small molecule technology, applied in the field of extraction of small molecule Polygonatum chinensis polypeptides, can solve the problems of low protein extraction efficiency, low content of small molecule polypeptides, wide product molecular weight distribution, etc., to improve the utilization rate, improve the extraction rate, reduce the effect of dosage

Active Publication Date: 2019-03-08
河北肽都生物科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of low protein extraction efficiency, low content of extracted small molecule polypeptide and wide molecular weight distribution in the existing small molecule Polygonatum polypeptide extraction process, the present invention provides a method for extracting small molecule Polygonatum polypeptide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A kind of extraction method of small molecular Polygonatum Polypeptide:

[0038] Step 1. Select 1000g of dried Polygonatum, cut into slices with a slicer, add 7000ml of purified water, stir evenly, and decoct at 90°C for 3 hours to obtain a decoction mixture;

[0039] Step 2: Cool down the decoction mixture to 55°C, add 1g of cellulase, 10g of amylase and 5g of glucoamylase, stir and enzymolyze at a speed of 60r / min for 2.0h to obtain the first enzymatic hydrolysis solution;

[0040] Step 3, desugar the first enzymolysis solution with a decanter centrifuge, the speed of the decanter centrifuge is 3700r / min, add 3000ml of purified water to the desugared Polygonatum tablets, heat up to 50°C, add 5g of exo-Aspergillus oryzae neutral protease, 10g actinidia protease and 1g ginger protease, stirred for 3.0h to obtain the second enzymolysis solution;

[0041] Step 4: Warm up the second enzymolysis solution to 90°C, and inactivate the enzyme for 15 minutes; centrifuge and pur...

Embodiment 2

[0045] A kind of extraction method of small molecular Polygonatum Polypeptide:

[0046] Step 1. Select 10Kg of dried Polygonatum, slice it with a slicer, add 75L of purified water, stir evenly, and decoct at 92°C for 2.5 hours to obtain a decoction mixture;

[0047] Step 2: Cool down the decoction mixture to 57°C, add 20g of cellulase, 120g of amylase and 70g of glucoamylase, and stir at a speed of 65r / min for 2.5h to obtain the first enzymolysis solution;

[0048] Step 3. Centrifuge the first enzymatic hydrolysis solution with a decanter centrifuge to desugar. The rotational speed of the decanter centrifuge is 4000r / min. Add 35L of purified water to the desugared Polygonatum tablets, heat up to 52°C, and add 70g of exoaspergillus oryzae neutral protease, 120g actinidia protease and 20g ginger protease, stirred for 3.5h to obtain the second enzymolysis solution;

[0049] Step 4. The temperature of the second enzymolysis solution is raised to 92°C, and the enzyme is deactivate...

Embodiment 3

[0053] A kind of extraction method of small molecular Polygonatum Polypeptide:

[0054] Step 1. Select 100Kg of dried Polygonatum, slice it with a slicer, add 800L of purified water, stir evenly, and decoct for 2 hours at 95°C to obtain a decoction mixture;

[0055] Step 2: Cool down the decoction mixture to 58°C, add 300g of cellulase, 1.5Kg of amylase and 800g of glucoamylase, and stir the enzymolysis at a speed of 60r / min for 3.0h to obtain the first enzymolysis solution;

[0056] Step 3. Centrifuge the first enzymatic hydrolysis solution with a decanter centrifuge to desugar. The rotational speed of the decanter centrifuge is 3500r / min. Add 400L of purified water to the desugared Polygonatum tablets, heat up to 55°C, and add 800g of exoaspergillus oryzae neutral protease, 1.5Kg actinidia protease and 300g ginger protease, stirring and enzymolysis for 4.0h, to obtain the second enzymolysis solution;

[0057] Step 4: Warm up the second enzymolysis solution to 95°C, and inac...

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Abstract

The invention provides an extraction method of small molecular rhizoma polygonati polypeptide. The extraction method comprises the following steps: step 1, slicing rhizoma polygonati and adding purified water; uniformly stirring and decocting to obtain a decocted mixture; step 2, cooling the decocted mixture to 55 to 58 DEG C; adding cellulase, amylase and a saccharifying enzyme, and stirring andcarrying out enzymolysis to obtain a first enzymolysis solution; step 3, centrifuging the first enzymolysis solution and separating to obtain desugared rhizoma polygonati slices; adding purified waterinto the desugared rhizoma polygonati slices; raising the temperature to 50 to 55 DEG C and adding exoaspergillus oryzae neutral protease, fresh ginger protease and kiwi fruit protease; stirring andcarrying out enzymolysis to obtain a second enzymolysis solution; step 4, heating the second enzymolysis solution and raising the temperature to carry out enzyme inactivation; separating and purifyingto obtain the small molecular rhizoma polygonati polypeptide. The extraction method provided by the invention adopts a two-time enzymolysis method of a compound enzyme, and a centrifuging technologyand a nano-filtering technology are combined, so that a prepared rhizoma polygonati peptide product has high purity and the content of the peptide with a peptide molecular weight of 180 to 500 Daltonsreaches 99 percent or above.

Description

technical field [0001] The invention relates to the technical field of separation of biological peptides, in particular to a method for extracting small molecule Polygonatum polypeptide. Background technique [0002] Polygonatum, also known as: Chicken Head Polygonatum, Yellow Chicken Vegetable, Pen Tube Vegetable, Claw Ginseng, Tiger Ginger, Chicken Claw Ginseng, is a plant of the genus Polygonatum. Polygonatum contains protein, saponins, niacin, sugars, quinones, amino acids and Various ingredients such as trace elements are mainly used for yin deficiency and dryness of the lung, dry cough with less phlegm, chronic cough due to lung and kidney yin deficiency, weakness of the spleen and stomach, kidney deficiency and essence deficiency, etc. Most sealworts on the market are half-boiled and dried directly in the sun. Although it is easy to preserve and does not consume too much weight, the macromolecular proteins in sealwort cannot be effectively absorbed and utilized by the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06
Inventor 张晓东杨广杨子丰
Owner 河北肽都生物科技集团有限公司
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