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Method for pretreating lignocellulose by utilizing cold plasmas

A lignocellulose and cold plasma technology, which is applied in fermentation and other directions, can solve the problems of slow processing speed, high cost of pretreatment process, low temperature required, etc., and achieves a pollution-free environment, high efficiency and low energy consumption. Effect

Inactive Publication Date: 2016-02-03
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Biological pretreatment is the use of microorganisms to degrade lignin. This method does not require the use of chemicals and requires low temperature. It is an energy-saving and environmentally friendly method, but the processing speed is too slow
And the existing above-mentioned pretreatment methods all have the defect that the pretreatment process occupies high cost, reached 20% of the total conversion cost, so there is an urgent need for economical, efficient and environmentally friendly new pretreatment methods

Method used

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  • Method for pretreating lignocellulose by utilizing cold plasmas

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Remove the sundries in the rice straw, then wash the straw, dry it at 50°C to remove water, and then use a ball mill to crush it to more than 150 mesh to obtain the crushed lignocellulose raw material, and then add the lignocellulose raw material to the medium In the barrier discharge plasma device, start the machine and warm up for 6 minutes, with O 2 The atmosphere is used as the modification treatment atmosphere, O 2 The flow rate is 0.05L / min, the regulating voltage is 30v to obtain a processing power of 300W, and the modification pretreatment time is 20s.

[0019] Use cellulase to hydrolyze the modified product, the amount of enzyme added is 35U / g (to the dry weight of cellulose), the enzymolysis temperature is 40°C, pH4.6, the enzymolysis time is 96 hours, and the glucose conversion determined by high performance anion exchange chromatography Its cellulase hydrolysis yield is 45.4%.

Embodiment 2

[0024] Remove the sundries in the corn stalks, then wash the stalks, dry them at 50°C to remove moisture, and use a ball mill to crush them to more than 150 mesh to obtain crushed lignocellulose raw materials, and then add lignocellulose raw materials to the medium In the barrier discharge plasma device, start up and warm up for 7 minutes, and use N 2 The atmosphere is used as the modification treatment atmosphere, N 2 The flow rate is 0.06L / min, the regulating voltage is 20v to obtain a processing power of 300W, and the modification pretreatment time is 30s.

[0025] Use cellulase to hydrolyze the modified product, the amount of enzyme added is 35U / g (to the dry weight of cellulose), the enzymolysis temperature is 40°C, pH4.6, the enzymolysis time is 96 hours, and the glucose conversion determined by high performance anion exchange chromatography Its cellulase hydrolysis yield is 48.5%.

Embodiment 3

[0027] Remove the sundries in the rice straw, then wash the straw, dry it at 50°C to remove water, and then use a ball mill to crush it to more than 150 mesh to obtain the crushed lignocellulose raw material, and then add the lignocellulose raw material to the medium In the barrier discharge plasma device, start the machine and warm up for 6 minutes, with O 2 The atmosphere is used as the modification treatment atmosphere, O 2 The flow rate is 0.05L / min, the regulating voltage is 50v to obtain a processing power of 300W, and the modification pretreatment time is 40s.

[0028] Use cellulase to hydrolyze the modified product, the amount of enzyme added is 35U / g (to the dry weight of cellulose), the enzymolysis temperature is 40°C, pH4.6, the enzymolysis time is 96 hours, and the glucose conversion determined by high performance anion exchange chromatography Its cellulase hydrolysis yield is 57.6%.

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Abstract

The invention discloses a method for pretreating lignocellulose by utilizing cold plasmas. The method is characterized by adding the raw material lignocellulose to a dielectric barrier discharge plasma device and carrying out pretreatment by respectively using N2 / pure air mixed atmosphere, N2 atmosphere, O2 atmosphere or Ar atmosphere as modified treatment atmosphere. The method for pretreating lignocellulose by utilizing cold plasmas has the beneficial effects that the pretreatment process has the advantages of simplicity and easiness in operation, no environmental pollution, low energy consumption, high efficiency, and the like; and meanwhile, the lignocellulose enzymolysis rate is increased.

Description

technical field [0001] The invention relates to a method for pretreating lignocellulose with cold plasma, belonging to the field of cellulose pretreatment. Background technique [0002] A key step in the production of cellulosic ethanol is the hydrolysis of cellulose into simple sugars, which are further converted into ethanol. The hydrolysis of cellulose to glucose by cellulase is recognized as the most promising method. However, the barrier against degradation is a major problem in the conversion of lignocellulose to bioethanol. Lignocellulosic raw materials are mainly composed of three major components: cellulose, hemicellulose and lignin. Hemicellulose is used as a molecular adhesive to bind between cellulose and lignin, embedding cellulose molecules in it, forming a natural The barrier makes it difficult for enzymes to contact with cellulose molecules, resulting in slow enzymatic hydrolysis of polysaccharides in lignocellulosic raw materials. Therefore, in order to r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 徐徐陈莉晶王石发张瑜王佳瑜曹晓琴芮坚
Owner NANJING FORESTRY UNIV
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