Method for extracting collagen polypeptide from fish scale

A collagen peptide, fish scale collagen technology, applied in chemical instruments and methods, animal/human proteins, specific peptides, etc., can solve the problems of incomplete hydrolysis, waste of nutrients, high content of heavy metals, and achieve high stability, The effect of promoting absorption and reducing the content of heavy metals

Inactive Publication Date: 2019-02-01
新昌县赛因斯生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Prior art, such as the Chinese invention patent with the authorized notification number CN 101297673 B, discloses a processing method of fish collagen oligopeptides. The dried fish scales that have been delimed and degreased are selected as raw materials, and are cleaned, pretreated, enzymatically hydrolyzed, and filtered. , decolorization, deodorization, desalination, sterile filtration, concentration, and spray drying to obtain fish collagen oligopeptide powder with protein content ≥ 95%, ash content < 0.5%, and average molecular weight of 700Dalton on a dry basis; its processing The products produced are of high quality, good quality, colorless, no bitter taste, no fishy smell,

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  • Method for extracting collagen polypeptide from fish scale

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Embodiment 1

[0025] The method for extracting collagen peptides from fish scales includes: pretreatment, extraction, hydrolysis, metal removal, and purification. The specific steps are as follows:

[0026] Pretreatment: Take fish scales, wash with clean water, dry at low temperature, smash, soak in 1% citric acid solution for 24 hours, stir intermittently, change the solution once every 12 hours, rinse with deionized water to neutrality after soaking, and filter; then Soak in 0.03% sodium hydroxide solution for 24 hours, stirring intermittently, and change the solution once every 12 hours. After soaking, rinse with deionized water to neutrality and filter; pretreatment can remove calcium and non-collagen substances in fish scales to facilitate Further extraction of collagen;

[0027] Extraction: Place the pretreated fish scales in a 0.05mol / L citric acid solution system, add 0.3% papain restriction enzyme digestion of the substrate weight, control the temperature at 5°C, the ultrasonic frequenc...

Embodiment 2

[0034] The method for extracting collagen peptides from fish scales includes: pretreatment, extraction, hydrolysis, metal removal, and purification. The specific steps are as follows:

[0035] Pretreatment: Take fish scales, wash with clean water, dry at low temperature, crush, soak in 1% citric acid solution for 32 hours, stir intermittently, change the solution every 16 hours, rinse with deionized water to neutrality after soaking, and filter; then Soak in 0.04% sodium hydroxide solution for 32 hours, stirring intermittently, and change the solution every 16 hours. After soaking, rinse with deionized water to neutrality and filter; pretreatment can remove calcium and non-collagen substances in fish scales to facilitate Further extraction of collagen;

[0036] Extraction: Place the pretreated fish scales in a 0.06mol / L citric acid solution system, add 0.4% papain restriction enzyme hydrolysis of the substrate weight, control the temperature at 6°C, the ultrasonic frequency at 35kH...

Embodiment 3

[0043] The method for extracting collagen peptides from fish scales includes: pretreatment, extraction, hydrolysis, metal removal, and purification. The specific steps are as follows:

[0044] Pretreatment: Take fish scales, wash with clean water, dry at low temperature, crush, soak in 2% citric acid solution for 36 hours, stir intermittently, change the solution once in 18 hours, rinse with deionized water to neutrality after soaking, and filter; then Soak in 0.05% sodium hydroxide solution for 36 hours, stir intermittently, and change the solution once 18 hours. After soaking, rinse with deionized water to neutrality and filter; pretreatment can remove calcium and non-collagen substances in fish scales to facilitate Further extraction of collagen;

[0045] Extraction: Place the pretreated fish scales in a 0.08mol / L citric acid solution system, add 0.5% papain restriction enzyme digestion of the substrate weight, control the temperature at 10°C, the ultrasonic frequency at 45kHz, ...

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Abstract

The invention discloses a method for extracting collagen polypeptide from fish scale. The method comprises the following steps: pretreatment, extraction, hydrolysis, metal removal, and purification; wherein a hydrolysis step is characterized by comprising the following steps: fish scale collagen and subtilisin are added to distilled water and the materials are completely dissolved, t-butylhydroperoxide and stevioside are added, the pH value is adjusted, and double-frequency ultrasonic assisted enzymatic hydrolysis is carried out on an enzymatic hydrolysate in a constant temperature water bath;a trace amount of t-butylhydroperoxide and stevioside generate synergism, which can increase the degree of hydrolysis of collagen and increase the enzymatic hydrolysis rate of subtilisin; wherein a metal removal step is characterized by comprising the following steps: starch xanthate and mulberry polysaccharide are added to the enzymatic hydrolysate, and the steps of mixing, reacting, and filtering through diatomite are carried out to obtain fish scale collagen polypeptide clear liquid; and the starch xanthate and mulberry polysaccharide have higher removal rates of heavy metal ions. The method has the beneficial effects that the method for extracting the collagen polypeptide from fish scale provided by the invention has the advantages of easy control, complete hydrolysis, large proportion of polypeptide in the product, easy absorption by the body, and low content of heavy metals.

Description

Technical field [0001] The present invention relates to the technical field of collagen polypeptide extraction, in particular to a method for extracting collagen polypeptide from fish scales. Background technique [0002] Collagen is widely present in connective tissues such as animal skin, bone, tendon, and membrane, and is the main component of connective tissue. In the past, the production of collagen mainly used resource-rich terrestrial animals, such as the skin and bones of cattle and pigs as raw materials. Although fish collagen has long been recognized by humans, such as tortoise shell glue, yellow fish glue, etc., it has not been used as an important industrial application material. This is mainly a product that relies on marine fishing, and the source is unstable and lacks scale. In recent years, as terrestrial animals are affected by various factors such as the environment and infectious diseases, the safety of their proteins has been questioned. However, aquaculture ...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/14C12P21/06
CPCC07K14/78C12P21/06
Inventor 徐康
Owner 新昌县赛因斯生物科技有限公司
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