Heparin sodium crude product refining optimization technology
A technology for heparin sodium and crude products, applied in the biological field, can solve problems such as difficulty in obtaining high-quality heparin sodium products, unsuitable reaction conditions, and structural damage of heparin sodium
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[0004] The implementation of the present invention will be illustrated by specific specific examples below, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. An optimization process for refining crude heparin sodium, through crushing and screening, dissolving, enzymatic hydrolysis, filtration, oxidation and precipitation, drying and dehydration, the process for refining and optimizing crude heparin sodium is completed, and the specific steps are as follows: (1) Crushing and screening: the crude heparin sodium is first Grinding, then sieving, and crushing the larger particles again; (2) Dissolving: Put the pretreated crude heparin sodium into water, add sodium chloride solution, and stir with a mixer; (3) Enzymolysis : Add human trypsin to the dissolved mixed liquid, adjust the pH value to 8.2-9, and heat to 40-60°C, and the enzymolysis time is 4-8 hours; (4) Filtration: hea...
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