129 results about "Enzyme activator" patented technology

The invention provides compositions and methods for the detection and/or quantification of biological targets (e.g., nucleic acids and proteins) by the nucleic acid-templated creation of one or more reaction products, for example, epitopes, enzyme substrates, enzyme activators, and ligands. The reaction products can be detected and/or quantitated after signal amplification using an amplification system.

A polypeptide selected from the group of lysosomal enzymes and lysosomal enzyme activators, comprising at least one introduced glycosylation site as compared to a corresponding parent enzyme or activator. By introducing additional glycosylation sites the resulting glycosylated lysosomal enzyme or activator obtains improved in vivo activity and thereby provides for improved treatment of lysosomal storage diseases.

A method for producing glucose and cellobiose using cellulose complex enzyme is the technology using the cellulose complex enzyme as a catalyst and realizing the high saccharification rate conversion of cellulose by adding cellulose enzyme activator at the optimized stirring speed. Glucose concentration can reach 150g/L after using cellulose enzymatic hydrolysis, and the method is a cellulose saccharification production technology with economic feasibility. With the technology used, low cost, low usage of enzyme and high saccharification rate can be realized, and the productions of cellulose alcohol and biochemical products (lactic acid, succinic acid and the like) are satisfied. In the whole technology, no beta-glucosaccharase is added additionally, no sugar concentrating device is added, and the technology is simple, the equipment cost is low and the industrialized prospect is good.

A method for regulating Src and its downstream signaling pathway which includes binding between Src and Na+/K+-ATPase is disclosed. The Na+/K+-ATPase/Src complex is a functional receptor for cardiotonic steroids such as ouabain. Also disclosed are Src inhibitors or activators which include either Na+/K+-ATPase or Src that interfere with the interaction between the Na/K-ATPase and Src, act via a different mechanism from ATP analogues, and is pathway (Na+/K+-ATPase) specific.

The invention discloses a preparing method of bacillus subtilis TQS6 CCTCC M207001 of highly effective expression neutral beta-mannase through adopting UV (ultraviolet mutagenesis ) and NTG (nitrosoguanidine mutagenesis) compositing mutagenesis, which comprises the following steps: bevel-culturing bacterial strain; proceeding ferment culture after one grade and two grade culture; choosing culture medium; adding into not exclusively degraded liquid from centrifuging sugar liquid; venting into oxygen-enriched air during the course of yeast; increasing dissolved oxygen; getting high active neutral beta-konjak mannase. The product possesses high enzyme production, high enzyme activator, short period of production and low cost, which can invert low mannan effectively.

The invention provides methods for treating or decreasing the likelihood of developing a stress-granule related disorder and/or cancer by administering one or more poly-ADP-ribose polymerase (PARP) inhibitors, one or more PARP activators, one or more poly-ADP-ribose glycosylase (PARG) activators, and/or one or more poly-ADP-ribose glycohydrolase ARH3 activators. The invention also provides corresponding methods of decreasing stress granule formation and/or proliferation in a cell or a population of cells. The invention further provides methods of increasing the number of stress granules and proliferation in a cell or a population of cells by administering one or more PARP activators, one or more PARP inhibitors, one or more PARG inhibitors, and/or one or more ARH3 inhibitors. The invention also provides methods for screening for agents for treating or decreasing the likelihood of developing a stress granule-related disorder or cancer, and methods for determining the propensity for developing a stress granule-related disorder or cancer, as well as compositions and kits containing one or more PARP inhibitors, one or more PARP activators, one or more PARG activators, and one or more ARH3 activators.

The invention discloses a deinking method of waste paper alkaline enzyme, comprising the following steps: carrying out soaking, disintegration, enzyme processing and washing on the waste paper to obtain the processed pulp; adding surface active agent when soaking at a usage amount of 0.10%-2.00%wt, wherein the enzyme processing is carried out under the conditions that the pH is 6-11, the temperature is 45-75 DEG C, and xylanase 30-150 mu/g of oven dry stock, pectinase 40-100 mu/g of oven dry stock and amylase 50-120 mu/g of oven dry stock are adopted to process the disintegrated waste paper for 10-30min. Cellulase can also be added in the enzyme processing procedure with an added quantity of 100 mu/g of oven dry stock. The processing effect is better if enzyme activator, such as sodium chloride is added in the enzyme processing procedure; and the alkaline enzyme can be bathed and used in a matching manner with a plurality of surface active agent, even with compounds with certain oxidation functions; and the process of waste paper deinking technology can be shortened and the bleaching process in deinking by the conventional enzyme method can be left out.

The invention discloses a resveratrol analogue and a preparation method and application thereof to treatment of diseases related to aging and SIRTI enzyme activity. The resveratrol analogue disclosed by the invention has an eliminating effect on DPPH radical (DPPH.), superoxide anion radical (O2<.->), hydroxide anion radical (OH.) and nitrogen peroxide radical (OHOO.) and has excellent antioxidation activity. Some derivatives of the resveratrol analogue are SIRTI enzyme activating agents while others are excellent SIRT1 enzyme inhibitors.

The invention discloses a medical stone dual-effect multi-element silica, calcium and potassium fertilizer and a preparation method thereof. The medical stone dual-effect multi-element silica, calcium and potassium fertilizer is produced by organically blending medical stone, calcium stone, bentonite, attapulgite, potassium oxide, magnesium sulfate, borax, zinc sulfate, magnesium sulfate, ferrous sulfate, aluminum potassium sulfate, agricultural traditional Chinese medicines (radish seed, celastrus angulatus, garlic juice, tobacco stalk, castor bean, onion juice and liquorice), a biological enzyme activator, a polyaspartic acid (PASP) plant nutrient accelerator, sodium nitrophenolate and the like. The preparation method breaks through the classic production technology frames home and abroad and comprises the following steps of: under the condition of normal temperature and normal pressure, crushing and milling the raw materials in the formula into powder of which the grain size is about 120 to 200 meshes; uniformly mixing the powder in a stirrer according to proportion; conveying the mixture to a granulator through a conveyor; after spraying and chelating the mixture through PASP and water mixed solution, granulating the mixture; after curing and drying the mixture with a dryer at high temperature, screening the grains, wherein the fertilizer product is 2.5 to 4.5mm in grain size, 5 to 6 Newtons in strength and less than 5 percent in free moisture; and after the product is checked to be qualified, metering and filling the product in bags. Fertilizer and medicine are integrated in the product; the product is comprehensive in nutrient elements, uniformly supplies fertilizer and is rich in organic substances and amino acids; residual fertilizer in the soil can be activated and used; the yield and the quality of crops are improved; green and organic agricultural products are produced; income of farmers is increased; the defect and difficulty brought to agricultural production and plantation by applying chemical fertilizers for a long time can be well overcome; the defects and the disadvantages of the chemical fertilizers are overcome; and the industry policy requirement of China on the economic benefit and social benefit of a green, organic and environment-friendly sustained or controlled release fertilizer is really met.

The invention provides a method for extracting okra polysaccharides. The method specifically comprises the following steps: 1) performing pretreatment; 2) degreasing; 3) performing enzymolysis; 4) purifying; and 5) deproteinization. The method has the beneficial effects that the polysaccharides are extracted by compound enzymolysis in a microwave solubilization manner. The enzymolysis conditions are mild, and the structure of the okra polysaccharide is not damaged, so that the okra polysaccharide remains high activity; multiple enzymes are subjected to combined enzymolysis, the compound enzymes are activated by multiple enzyme activators, the enzymolysis is high in speed and complete, and the extraction rate of the okra polysaccharides is improved; by combining solvent extraction and microwave extraction, the extraction rate of the okra polysaccharides can be improved; chloroform and n-butyl alcohol can denature the protein to be water-insoluble, the chloroform and n-butyl alcohol are added into a polysaccharide solution containing proteins so as to form gel, and then the protein is removed in a centrifugal manner; the finally obtained okra polysaccharide is high in purity, and the okra polysaccharide is high in extraction rate and can remain high activity; and the operation steps are simple, the cost is low, and the economic benefits are high.

The invention discloses a high-protein distiller's grain feed and a preparation method thereof. With distiller's grain and silkworm pupa powder as main materials, the preparation method for the high-protein distiller's grain feed utilizes a variety of strains ( such as phanerochaete chrysosporium, trichoderma reesei and aspergillus niger) to carry out step-by-step fermentation, and thereby lignin,cellulose and hemicellulose in distiller's grain are deeply degraded in succession. The invention effectively controls factors (such as choice and proportioning of carbon source and nitrogen source,oxygen input, intermediate degradation product, enzyme activator and inhibitor) in the process of microbial fermentation degradation, increasing the degradation rate of crude fibers, the utilization rate of distiller's grain and the feeding value of the distiller's grain feed. According to the high-protein distiller's grain feed prepared by the fermentation method disclosed by the invention, the crude fiber content is decreased by 12 to 15 percent, the crude protein content is increased by 25 to 28 percent, and the nutrition of the high-protein distiller's grain feed is closer to the nutritionof complete feed.

The invention provides an extraction method of small molecule liver peptide. The extraction method comprises following steps: step one, animal liver is mashed, purified water is added, a mixed liquidis stirred uniformly and heated to 54-58 DEG C, lipase and neutral protease are added, stirring enzymolysis is performed for 25-35 min, and a first enzymatic hydrolysate is obtained; step two, flavourzyme, chicken intestinal digestive tract protease and an enzyme activator are added to the first enzymatic hydrolysate and stirred uniformly, a mixture is cooled to 15-20 DEG C and subjected to enzymolysis extraction 2-3 times, enzymolysis time is 1-1.5 h each time, all extracting solutions are combined, and a second enzymatic hydrolysate is obtained; step three, the second enzymatic hydrolysate is heated and subjected to enzyme inactivation, a product is separated, purified, concentrated and lyophilized, and lyophilized small molecule liver peptide powder is obtained. The prepared liver peptide product is high in purity and can be widely applied to the fields of food, healthcare products, drugs and the like, and content of peptide with molecular weight being 180-500 Dalton can reach 99% or higher.

The invention discloses a method for refining tea oil for cosmetics, and relates to the field of technologies for processing oil. The method for refining the tea oil includes five steps of degumming, deacidifying, decoloring, deodorizing and fractionating. Enzyme activators such as calcium dihydrogen phosphate, potassium chloride and ferrous sulfate are added at the step of degumming and are combined with ultrasonic treatment to assist in degumming; decoloring is carried out by the aid of modified activated carbon and activated clay jointly at the step of decoloring. The method for refining the tea oil has the advantages that degumming and decoloring processes are further optimized, so that the degumming time can be shortened, the quality of decolored oil can be improved, the tea oil obtained by the aid of the method is colorless and odorless and is high in stability, oxidation and color reversion of the tea oil can be prevented, and requirements on oil for the cosmetics can be met.

The invention relates to zymolytic humic acid for agriculture and a production method thereof and belongs to a humic acid and its production method. The production method solves the technical problem that the existing humic acid production method has poor activation effects and complex activation processes, produces strong corrosion to raw materials and has high energy consumption, a high cost and large environmental pollution. The zymolytic humic acid for agriculture is prepared from 100 parts by weight of weathered coal, 0.01-0.05 parts by weight of a compound enzyme activator and 15 parts by weight of an auxiliary agent. The production method comprises 1, preparing a compound enzyme activator, 2, preparing an auxiliary agent, and 3, preparing zymolytic humic acid through adjusting water content of 100 parts by weight of weathered coal to obtain coal paste with water content of 60-70%, adjusting pH through calcium hydrate, adding the compound enzyme activator into the coal paste, carrying out uniform mixing, conveying the mixture into a degradation tank, adding the auxiliary agent into the mixture, carrying out degradation, and crushing the mixture through a chain type pulverizer to obtain a zymolytic humic acid finished product.

The invention provides an extraction method of active ingredients of medicinal fungi. The extraction method comprises the following steps: (1) crushing raw materials of the medicinal fungi, wherein the diameters are 75-300 microns after the raw materials are crushed; (2) adding mixed enzymes and an enzyme activator into the crushed raw materials of the medicinal fungi to implement enzymolysis, wherein the dosage of the mixed enzymes is 1-3% of the raw materials of the medicinal fungi, the enzymolysis temperature is 20-45 DEG C, the pH value is 4-9, and enzymolysis reaction is carried out for 40-100 minutes; and (3) filtering the product subjected to enzymolysis and collecting extraction liquid to obtain the active ingredients of medicinal fungi. According to the extraction method provided by the invention, the yield is high, the energy consumption is low and the damage on the active ingredients of the medicinal fungi is alleviated, and the extraction time is remarkably shortened compared with that of the existing extraction method, and the content of triterpene and adenosine in the extract is improved to a greater extent compared with that of the existing extraction method, moreover, the operation is simple and the cost is relatively low.

The present invention discloses a wound healer for trees and an application thereof. The healer is a special preparation compounded by a plant growth regulator, a plant nutrient, a plant physiologically active substance, a bactericide, an enzyme activator and a filming protective agent so as to be similar with liquid in formative layer of bark. The present invention prepares the compound scientifically according to the characteristics of the trees and main components of the liquid in the formative layer, and solves the technical problems that wound heals slowly and the trees are injected easily; the healer enables the wound (by insects, diseases and machines) to heal and recover rapidly and form a new bark layer, can improve resistance of the trees, restores transportation of photosynthetic products in leaves and growth and vigor of the trees rapidly, achieves 100% of healing rate of the wound, and has a significant effect. Due to the fact that the healer is full of a variety of nutritional elements and is in a chelated state, the healer can be absorbed by plants quickly, and is also a micro fertilizer to promote the growth of the plant efficiently and increase yield. The product adapts injuries and disease wounds of various fruit trees and garden trees, and sprays on leaves of vegetables, fruits, legumes and other green plants of agricultural crops.

The invention belongs to the technical field of high polymer materials and water treatment, and discloses an MBBR (Moving Bed Biofilm Reactor) filler which comprises the following components: a hydrophilic substrate, a toughening agent, light calcium carbonate, a density regulator, biological nutritive salt, a biological active agent and a biological enzyme activator. The toughening agent is copolymer grafted maleic anhydride, and meanwhile, hollow glass beads are selected as the density regulator, so that the problems of high density of inorganic filler of a hydrophilic filler agent and interface between the inorganic filler and a base material are solved. The invention also discloses a preparation method of the MBBR filler. Compared with polyethylene fillers, the prepared MBBR filler hasbetter hydrophilicity and biocompatibility. When the MBBR filler is used in an MBBR process, the COD removal rate is more than 85%, the ammonia nitrogen removal rate is more than 95% and the effluentCOD is less than 30mg/L. The MBBR filler is suitable for treatment of various sewage with different carbon/nitrogen and ammonia/nitrogen ratios, and is high in efficiency, high in aging and strong inuniversality.