19 results about "Enzyme activator" patented technology

This invention discloses a quantitative detection kit for arginine peptidase, a qualitative detection kit for arginine peptidase, and their usage methods. The quantitative detection kit utilizes the specific substrate Nα-benzoyl-DL-arginine-4-ylbenzamide hydrochloride, which can be hydrolyzed by arginine peptidase to 4-ylbenzamide. 4-ylbenzamide has a maximum absorption peak at 405 nm, and the specific arginine peptidase activity can be rapidly detected at room temperature using spectrophotometry. The qualitative kit utilizes the specific substrate Nα-benzoyl-DL-arginine-β-naphthamide hydrochloride, which can be hydrolyzed by arginine peptidase to p-β-naphthamide. β-naphthamide combines with 4-(dimethylamino)cinnamaldehyde to form a compound that is visible to the naked eye as a pink color. The disulfide bond cleaving agent cuts the mucus disulfide bonds, exposing bacterial enzyme sites, and the synergistic effect of the enzyme activator enhances the reaction rate of arginine peptidase hydrolysis of the substrate, significantly improving the detection sensitivity of arginine peptidase in tongue coating samples.

Methods for preparing pyruvate kinase (PK) activators or salts or hydrates thereof are provided herein. Synthetic methods for preparing pyruvate kinase (PK) activators, or salts or hydrates thereof, are provided herein. Such activators may be prepared using compounds having formula (A-1) and / or formula (B-1). Specific PK activators and various intermediates prepared by and / or used by the synthetic methods described herein are also disclosed.

This invention relates to the field of pharmaceutical technology, specifically the application of NEDD8 activator in the preparation of drugs for treating Japanese encephalitis. Using human neuroblastoma cells (SH-SY5Y) as target cells, this invention utilizes activators from a library of ubiquitinated compounds to target and activate key enzymes in the ubiquitination pathway, aiming to identify host factors associated with Japanese encephalitis virus (JEV) infection of SH-SY5Y cells. This helps to understand the mechanism by which JEV invades the central nervous system and causes neuronal cell damage, and also provides new targets for therapeutic drugs against JEV-induced Japanese encephalitis. This invention experimentally discovered that NEDD8 activator (NAE) has the property of inhibiting JEV infection of SH-SY5Y cells. This invention provides the application of NEDD8 activator in the preparation of drugs for treating Japanese encephalitis, offering new targets and treatment strategies for the prevention and treatment of JEV.

This invention discloses an anti-hair loss hair root mask, its preparation method, and its application, relating to the field of cosmetic technology. The anti-hair loss hair root mask comprises the following components by weight percentage: 4.0%~9.0% anti-hair loss composition; the anti-hair loss composition comprises the following raw materials: plant extracts, enzyme activators, and a reconstitution system; the enzyme activator is selected from at least one of the following: β-glucosidase derived from Aspergillus niger, β-glucosidase derived from almonds, cellulase complex enzyme, malt extract, and citrus fruit fermentation broth; the plant extract includes Platycladus orientalis leaf extract. The anti-hair loss hair root mask, its preparation method, and its application of this invention achieve a significant increase in raw material utilization and anti-hair loss efficacy through targeted enzymatic release of active ingredients; the innovative product form ensures stable enzyme activity and precise efficacy; it also has scalp oil control and soothing effects and is easily mass-produced industrially.

This invention discloses an anti-hair loss composition, its preparation method, and its application, relating to the field of cosmetic technology. The anti-hair loss composition comprises the following raw materials: plant extracts, enzyme activators, and a reconstitution system; the enzyme activator is selected from at least one of the following: β-glucosidase from Aspergillus niger, β-glucosidase from almonds, cellulase complex enzyme, malt extract, and citrus fruit fermentation broth; the plant extract includes Platycladus orientalis leaf extract; the reconstitution system includes a moisturizing stabilizer, a chelating agent, a buffer system, and deionized water. The anti-hair loss composition, its preparation method, and its application of this invention, through targeted enzymatic hydrolysis technology, efficiently convert inactive glycoside precursors in Platycladus orientalis leaf extract into free flavonoids with targeted anti-hair loss activity, significantly improving raw material utilization and active ingredient yield, achieving a technological breakthrough from "passively adding raw materials" to "actively generating active substances."

The present invention provides small molecule PAK1 (P21 Activated Kinase 1) and / or PAK2 (P21 Activated Kinase 2) activators and their use in the prevention and / or treatment of diseases or conditions responsive to PAK1 and / or PAK2 activity or diseases or conditions associated with PAK1 and / or PAK2 inactivity, e.g. cardiovascular diseases or conditions, including cardiac fibrosis, cardiac hypertrophy, cardiac arrhythmias, heart failure and ischaemia / reperfusion injury.

The invention discloses a compound beverage and a preparation method thereof, and belongs to the technical field of beverage preparation. The compound beverage is prepared by the following steps: step 1, mixing and fermenting angelica sinensis, codonopsis pilosula, lotus seeds, phaseolus calcaratus, poria cocos, day lily, coix seeds, Chinese wolfberry fruits, Chinese dates and stevia rebaudiana; step 2, carrying out mixed fermentation on the Taihe black chicken meat, the pig trotters, the crucian meat, the donkey-hide gelatin powder, the compound enzyme and the enzyme activator; and step 3, mixing the materials obtained in the step 1 and the step 2 to obtain the compound beverage. Compared with the prior art, the prepared compound beverage has the advantages of being rich in nutritional ingredients, good in stability, high in protein and amino acid content, resistant to oxidation, free of peculiar smell after being added with meat ingredients and the like.

The present invention aims to provide a novel AMP-activated protein kinase activator.The present invention relates to a compound represented by the formula (I):wherein each symbol is as described in the specification, or a salt thereof, or a hydrate thereof. In addition, the present invention relates to an AMP-activated protein kinase activator containing the aforementioned compound, and a medicament containing the aforementioned compound for the prophylaxis and / or treatment of cancer.

The present invention discloses a method for preparing fish collagen peptides by enzymatic hydrolysis after biological fermentation, and relates to the field of biotechnology. The preparation method of the fish collagen peptide comprises the following steps: (1) adding an extractant to fish tissue for extraction, filtering, and obtaining a filtrate; (2) adding a carbon source to the filtrate to obtain a fermentation medium; (3) inoculating a plant lactobacillus strain on a seed culture medium, culturing on a shaking table, and obtaining a seed liquid; (4) inoculating the seed liquid into a fermentation medium, and fermenting and culturing; (5) first adding an enzyme activator, then adding an enzymolysis agent for enzymatic hydrolysis, inactivating the enzyme, centrifuging, and drying to obtain fish collagen peptide powder; the enzyme activator described in step (5) is a fungus polysaccharide, oligomannose, and raffinose. The preparation method of the present invention ensures that the prepared fish collagen peptide has high quality and good antibacterial effect, can effectively improve symptoms such as skin sagging, dull color, greasy, and freckles, and has good application prospects.

This invention discloses a method and application for extracting active ingredients from Platycladus orientalis leaves based on enzymatic reactions, relating to the field of cosmetic technology. The method includes the following steps: S1. Providing Platycladus orientalis leaf extract; S2. Providing an enzyme activator, wherein the enzyme activator is selected from at least one of the following: β-glucosidase from Aspergillus niger, β-glucosidase from almonds, cellulase complex enzyme, malt extract, and citrus fruit fermentation broth; S3. Mixing the enzyme activator with the Platycladus orientalis leaf extract to carry out an enzymatic reaction, thereby obtaining Platycladus orientalis leaf active ingredients based on the enzymatic reaction. The method and application of this invention for extracting Platycladus orientalis leaf active ingredients based on enzymatic reactions utilize diversified enzyme activator sources, balancing efficient conversion and compliant application. By optimizing the enzyme powder reconstitution system, stable storage of enzyme activity is ensured, and targeted and efficient conversion is achieved through precise enzymatic hydrolysis processes.

Disclosed herein are compounds with protein phosphatase 2A agonizing activity. In some embodiments, the compounds may be used in the treatment of cellular disorders, including cancer. In some embodiments, the compounds are used to treat colorectal cancer, including FOLFOX resistant colorectal cancer.

The present application relates to a kind of pueraria powder composition with fat-reducing effect and preparation method thereof, belong to fermentation product preparation technical field, including the following preparation steps: pueraria is classified and washed, gradient temperature pre-gelatinization is added, enzyme activator is adjusted pH value, constant temperature stirring is low pressure microburst, sterilization is optimized, and pueraria fermentation base material is prepared;Compound probiotics are activated by preparing pueraria base special activated culture medium, and compound bacterial liquid is prepared;Compound bacterial liquid is connected into pueraria fermentation base material and carries out synergistic anaerobic fermentation, after ripening, freezing granulation drying, sterile packaging, and pueraria powder composition is prepared.The technical scheme in the present application, by pueraria pretreatment process, active ingredients in pueraria are released and puerarin glycoside is realized, and human absorption rate is improved, while using the synergistic effect of compound probiotics and pueraria active ingredients, can effectively reduce weight, reduce abdominal fat accumulation, and overall improve the fat-reducing effect of pueraria powder composition.

The invention discloses a serum-free culture medium and application thereof in culture of large yellow croaker muscle stem cells. The serum-free culture medium comprises a basic culture medium and an exogenous additive component, wherein the exogenous additive components comprise HEPES, sodium chloride, hydroxypropyl-beta-cyclodextrin, sodium metavanadate, vitamin C magnesium phosphate, omega-3 polyunsaturated fatty acid, pyridoxine hydrochloride, nicotinamide, cholesterol, calcium chloride, potassium chloride, a soybean pancreatin inhibitor, taurine, trehalose, ethanolamine, a telomerase activator and recombinant human serum albumin. The animal-derived protein-free serum-free culture medium disclosed by the invention does not contain animal-derived components such as serum, does not need to add components such as growth factors or hormones, not only has definite chemical components, high safety, low cost and high cell proliferation speed, but also can effectively improve the myogenic differentiation potential of muscle stem cells and effectively activate telomerase activity to solve the problem of cell aging; and a feasible product and a feasible method are provided for culture of fish cells and large-scale production of cell culture meat.

The invention provides application of a glucosylceramide synthetase activator in preparation of a medicine for preventing and / or treating anxiety, and belongs to the field of medicine. According to the application disclosed by the invention, the glucosylceramide synthetase of the dorsal striatum is found to be an important regulatory protein for treating anxiety for the first time, and the key effect of the glucosylceramide synthetase in the dorsal striatum in anxiety mice induced by chronic constraint stress is determined by means of molecules, behavioristics and the like. It is found for the first time that the glucosylceramide synthetase agonist can significantly improve anxiety behaviors induced by chronic constraint stress, and the glucosylceramide synthetase agonist has wide application prospects in preparation of drugs for preventing and / or treating anxiety.

The application discloses an anti-inflammatory and antioxidant composition and a preparation method and application thereof, and relates to the technical field of cosmetics. The anti-inflammatory and antioxidant composition comprises the following components: an enzymatic biota orientalis extract and at least one of ginkgo biloba extract, mulberry leaf extract, bayberry leaf extract and sophora flower extract; the enzymatic biota orientalis extract is prepared through an enzymatic reaction of a biota orientalis extract, an enzyme activator and a redissolving system; the enzyme activator is selected from at least one of the following: beta-glucosidase from aspergillus niger, beta-glucosidase from apricot, cellulase complex enzyme, malt extract and citrus fruit fermentation liquor; and the redissolving system comprises a moisturizing stabilizer, a chelating agent, a buffer system and deionized water. The anti-inflammatory and antioxidant composition and the preparation method and application thereof have the effects of anti-inflammation, antioxidation and scalp barrier repair, and realize multi-effect synergy.

The invention provides a composite soaking agent for promoting synchronous seedling emergence of Chinese yam tubers as well as a preparation method and application of the composite soaking agent. The composite soaking agent is prepared from the following raw materials in parts by weight: 0.05 to 0.2 part of melatonin nano-liposome, 0.5 to 2.0 parts of sodium lignin sulfonate, 2 to 5 parts of garlic extracting solution, 5 to 10 parts of abscisic acid degrading enzyme activating agent, 3 to 8 parts of mitochondria activating agent, 2 to 6 parts of pyruvate kinase activating agent and 800 to 900 parts of purified water. The compound soaking agent solves the heterogeneity problem of tuber germination from multiple aspects through a five-in-one synergistic network of hormone regulation, energy enhancement, metabolism activation, oxidation resistance and bacterium resistance, and promotes tuber full seedling, so that integrated management of water, fertilizer and illumination in the later period is facilitated, the yield is increased, and the economic benefit is increased. A technical solution of a multi-target and multi-component synergistic mechanism is provided for high-quality and high-yield Chinese yam, and the method has remarkable application and popularization values.

Described herein are methods for using compounds that activate pyruvate kinase.

Hyodeoxycholic acid chenodeoxycholic acid

The invention relates to the technical field of medical detection, and discloses a platelet batroxobin activator A cup amplitude prediction method, device and equipment, the method comprises the following steps: obtaining a first detection parameter value of a common cup of a target detection sample, the first detection parameter value comprising fibrin formation time and / or fibrin polymerization rate; and based on the first detection parameter value of the target detection sample, predicting to obtain the maximum amplitude of the platelet batroxobin activator A cup of the target detection sample. The reagent cost for platelet inhibition rate detection can be remarkably reduced, and the detection efficiency is improved.