129 results about "Enzyme activator" patented technology

The invention provides compositions and methods for the detection and / or quantification of biological targets (e.g., nucleic acids and proteins) by the nucleic acid-templated creation of one or more reaction products, for example, epitopes, enzyme substrates, enzyme activators, and ligands. The reaction products can be detected and / or quantitated after signal amplification using an amplification system.

Disclosed is a multifunctional color bar enzymatic toothpaste and preparation method. The toothpaste is formed by one color regulation cream which containing chemical medicament additive and biologic enzyme activator, and multi-color cream containing biologic enzyme and biologic enzyme stabilizer. The preparation method is separately preparing regulation cream and enzyme cream and canning to color bar toothpaste. Functions of the toothpaste is to prevention and cure oral problem such as preventing and curing decayed tooth, diminishing inflammation, sterilization, desensitization, eliminating halitosis and whitening teeth. Simultaneously the toothpaste employing stabilizer and color bar to ensure the stability, and effectively exerting biology function of enzyme in release process by adding definite activator.

A method for producing glucose and cellobiose using cellulose complex enzyme is the technology using the cellulose complex enzyme as a catalyst and realizing the high saccharification rate conversion of cellulose by adding cellulose enzyme activator at the optimized stirring speed. Glucose concentration can reach 150g / L after using cellulose enzymatic hydrolysis, and the method is a cellulose saccharification production technology with economic feasibility. With the technology used, low cost, low usage of enzyme and high saccharification rate can be realized, and the productions of cellulose alcohol and biochemical products (lactic acid, succinic acid and the like) are satisfied. In the whole technology, no beta-glucosaccharase is added additionally, no sugar concentrating device is added, and the technology is simple, the equipment cost is low and the industrialized prospect is good.

A method for regulating Src and its downstream signaling pathway which includes binding between Src and Na+ / K+-ATPase is disclosed. The Na+ / K+-ATPase / Src complex is a functional receptor for cardiotonic steroids such as ouabain. Also disclosed are Src inhibitors or activators which include either Na+ / K+-ATPase or Src that interfere with the interaction between the Na / K-ATPase and Src, act via a different mechanism from ATP analogues, and is pathway (Na+ / K+-ATPase) specific.

The invention discloses a preparing method of bacillus subtilis TQS6 CCTCC M207001 of highly effective expression neutral beta-mannase through adopting UV (ultraviolet mutagenesis ) and NTG (nitrosoguanidine mutagenesis) compositing mutagenesis, which comprises the following steps: bevel-culturing bacterial strain; proceeding ferment culture after one grade and two grade culture; choosing culture medium; adding into not exclusively degraded liquid from centrifuging sugar liquid; venting into oxygen-enriched air during the course of yeast; increasing dissolved oxygen; getting high active neutral beta-konjak mannase. The product possesses high enzyme production, high enzyme activator, short period of production and low cost, which can invert low mannan effectively.

The invention relates to the field of an external diagnostic reagent, and particularly relates to a high-sensitivity kit for detecting 5'-nucleotidase. The kit for detecting 5'-nucleotidase comprises a 5'-nucleotidase R1 reagent, a 5'-nucleotidase R2 reagent and a 5'-nucleotidase calibration product, wherein the 5'-nucleotidase R1 reagent comprises a buffer solution, a stabilizing agent, a preservative agent, an enzyme activator, 4-AAP(4-aminoantipyrine), 18-crown ether-6 and UAO (Uricase), PNP (Purine Nucleoside Phosphorylase), XOD (Xanthine Oxidase) and POD (Peroxidase) reaction enzyme systems; the 5'-nucleotidase R2 reagent comprises a buffer solution, a stabilizing agent, a preservative agent, PO3- and a Trinder developing system; and the 5'-nucleotidase calibration product comprises a buffer solution, a stabilizing agent, a preservative agent and 5'-nucleotidase. The detection kit provided by the invention is enhanced in detection sensitivity by 40% compared with a 'PNP-XOD-POD' enzyme system, and has good application prospects.

The invention provides methods for treating or decreasing the likelihood of developing a stress-granule related disorder and / or cancer by administering one or more poly-ADP-ribose polymerase (PARP) inhibitors, one or more PARP activators, one or more poly-ADP-ribose glycosylase (PARG) activators, and / or one or more poly-ADP-ribose glycohydrolase ARH3 activators. The invention also provides corresponding methods of decreasing stress granule formation and / or proliferation in a cell or a population of cells. The invention further provides methods of increasing the number of stress granules and proliferation in a cell or a population of cells by administering one or more PARP activators, one or more PARP inhibitors, one or more PARG inhibitors, and / or one or more ARH3 inhibitors. The invention also provides methods for screening for agents for treating or decreasing the likelihood of developing a stress granule-related disorder or cancer, and methods for determining the propensity for developing a stress granule-related disorder or cancer, as well as compositions and kits containing one or more PARP inhibitors, one or more PARP activators, one or more PARG activators, and one or more ARH3 activators.

The present invention belongs to the field of food-derived peptides and discloses a collagen peptide, a production method, a production device and an application thereof. The production method of thecollagen peptide comprises steps of coarse collagen solution heating, cooling, high-efficiency compound enzyme preparation enzymatic hydrolyzing, membrane filtering, concentrating, ultra-high pressurehomogenizing, and ultra-high temperature instantaneous sterilizing and drying. The high-efficiency compound enzyme preparation enzymatic hydrolyzing uses a compound enzyme preparation comprising compound protease, alkaline protease, neutral protease, flavor protease, animal proteolytic enzyme and an enzyme activator, and the enzyme activator is dithiothreitol and / or beta-mercaptoethanol. The provided method does not need to adjust pH value of the enzymatic hydrolyzing, does not need to control enzymatic hydrolyzing temperatures, is short in enzymatic hydrolyzing time and small in enzyme addition amount, and enables the enzymatic hydrolyzed product to be low and concentrated in molecular weight. The prepared collagen peptide powder is free of fishy smell, extremely excellent in water resolubility and also not liable to absorb moisture and agglomerate during storage process, and can be used in solid beverages, effervescent tablets, chewable tablets, health-care foods and feature-beautifying and skin-protecting products.

The invention discloses a preparing method of feeding enzymolytic protein and shrimp case powder through waste shrimp head in the feeding additive preparing domain, which comprises the following steps: (1) squeezing the shrimp head to separate the case from meat; (2) breaking case and liquid meat; homogenizing; adding water to soak; (3) adding pH value buffer and enzyme activator; heating; stirring continuously; (4) condensing the filtrate; drying; obtaining the product. The invention saves cost to accelerate the manufacturing speed with high amino acid rate and stable quality, which utilizes the waste shrimp head sufficiently to avoid environment pollution.

The invention discloses a deinking method of waste paper alkaline enzyme, comprising the following steps: carrying out soaking, disintegration, enzyme processing and washing on the waste paper to obtain the processed pulp; adding surface active agent when soaking at a usage amount of 0.10%-2.00%wt, wherein the enzyme processing is carried out under the conditions that the pH is 6-11, the temperature is 45-75 DEG C, and xylanase 30-150 mu / g of oven dry stock, pectinase 40-100 mu / g of oven dry stock and amylase 50-120 mu / g of oven dry stock are adopted to process the disintegrated waste paper for 10-30min. Cellulase can also be added in the enzyme processing procedure with an added quantity of 100 mu / g of oven dry stock. The processing effect is better if enzyme activator, such as sodium chloride is added in the enzyme processing procedure; and the alkaline enzyme can be bathed and used in a matching manner with a plurality of surface active agent, even with compounds with certain oxidation functions; and the process of waste paper deinking technology can be shortened and the bleaching process in deinking by the conventional enzyme method can be left out.

The invention discloses a resveratrol analogue and a preparation method and application thereof to treatment of diseases related to aging and SIRTI enzyme activity. The resveratrol analogue disclosed by the invention has an eliminating effect on DPPH radical (DPPH.), superoxide anion radical (O2<.->), hydroxide anion radical (OH.) and nitrogen peroxide radical (OHOO.) and has excellent antioxidation activity. Some derivatives of the resveratrol analogue are SIRTI enzyme activating agents while others are excellent SIRT1 enzyme inhibitors.

The present invention relates to biological technology, and is especially biological enzyme activator for promoting the activity of biological enzyme. The biological enzyme activator has acetic acid as carrier and contains mixing of Ca ion in 14-18 wt%, Mg ion in 6.5-9 wt% and Mn ion in 0.5-1 wt%. The biological enzyme activator may find its wide application in agriculture, animal husbandry and other industry.

The invention discloses a medical stone dual-effect multi-element silica, calcium and potassium fertilizer and a preparation method thereof. The medical stone dual-effect multi-element silica, calcium and potassium fertilizer is produced by organically blending medical stone, calcium stone, bentonite, attapulgite, potassium oxide, magnesium sulfate, borax, zinc sulfate, magnesium sulfate, ferrous sulfate, aluminum potassium sulfate, agricultural traditional Chinese medicines (radish seed, celastrus angulatus, garlic juice, tobacco stalk, castor bean, onion juice and liquorice), a biological enzyme activator, a polyaspartic acid (PASP) plant nutrient accelerator, sodium nitrophenolate and the like. The preparation method breaks through the classic production technology frames home and abroad and comprises the following steps of: under the condition of normal temperature and normal pressure, crushing and milling the raw materials in the formula into powder of which the grain size is about 120 to 200 meshes; uniformly mixing the powder in a stirrer according to proportion; conveying the mixture to a granulator through a conveyor; after spraying and chelating the mixture through PASP and water mixed solution, granulating the mixture; after curing and drying the mixture with a dryer at high temperature, screening the grains, wherein the fertilizer product is 2.5 to 4.5mm in grain size, 5 to 6 Newtons in strength and less than 5 percent in free moisture; and after the product is checked to be qualified, metering and filling the product in bags. Fertilizer and medicine are integrated in the product; the product is comprehensive in nutrient elements, uniformly supplies fertilizer and is rich in organic substances and amino acids; residual fertilizer in the soil can be activated and used; the yield and the quality of crops are improved; green and organic agricultural products are produced; income of farmers is increased; the defect and difficulty brought to agricultural production and plantation by applying chemical fertilizers for a long time can be well overcome; the defects and the disadvantages of the chemical fertilizers are overcome; and the industry policy requirement of China on the economic benefit and social benefit of a green, organic and environment-friendly sustained or controlled release fertilizer is really met.

The invention provides a method for extracting okra polysaccharides. The method specifically comprises the following steps: 1) performing pretreatment; 2) degreasing; 3) performing enzymolysis; 4) purifying; and 5) deproteinization. The method has the beneficial effects that the polysaccharides are extracted by compound enzymolysis in a microwave solubilization manner. The enzymolysis conditions are mild, and the structure of the okra polysaccharide is not damaged, so that the okra polysaccharide remains high activity; multiple enzymes are subjected to combined enzymolysis, the compound enzymes are activated by multiple enzyme activators, the enzymolysis is high in speed and complete, and the extraction rate of the okra polysaccharides is improved; by combining solvent extraction and microwave extraction, the extraction rate of the okra polysaccharides can be improved; chloroform and n-butyl alcohol can denature the protein to be water-insoluble, the chloroform and n-butyl alcohol are added into a polysaccharide solution containing proteins so as to form gel, and then the protein is removed in a centrifugal manner; the finally obtained okra polysaccharide is high in purity, and the okra polysaccharide is high in extraction rate and can remain high activity; and the operation steps are simple, the cost is low, and the economic benefits are high.

The invention discloses a high-protein distiller's grain feed and a preparation method thereof. With distiller's grain and silkworm pupa powder as main materials, the preparation method for the high-protein distiller's grain feed utilizes a variety of strains ( such as phanerochaete chrysosporium, trichoderma reesei and aspergillus niger) to carry out step-by-step fermentation, and thereby lignin,cellulose and hemicellulose in distiller's grain are deeply degraded in succession. The invention effectively controls factors (such as choice and proportioning of carbon source and nitrogen source,oxygen input, intermediate degradation product, enzyme activator and inhibitor) in the process of microbial fermentation degradation, increasing the degradation rate of crude fibers, the utilization rate of distiller's grain and the feeding value of the distiller's grain feed. According to the high-protein distiller's grain feed prepared by the fermentation method disclosed by the invention, the crude fiber content is decreased by 12 to 15 percent, the crude protein content is increased by 25 to 28 percent, and the nutrition of the high-protein distiller's grain feed is closer to the nutritionof complete feed.

The invention discloses an environment-friendly straw fermenting agent and a preparation method thereof. The environment-friendly straw fermenting agent is prepared from the following raw materials inparts by weight: 26 to 30 parts of bacillus subtilis, 21 to 25 parts of trichoderma viride, 10 to 14 parts of saccharomyces cerevisiae, 10 to 14 parts of aspergillus niger, 1 to 5 parts of pectinase,5 to 8 parts of amylase, 1 to 3 parts of rayleigh mold, 5 to 10 parts of acid protease, 5 to 10 parts of neutral protease, 8 to 15 parts of arthrobacterium, 5 to 10 parts of cellulase, 1 to 3 parts of anti-aging agent, and 1 to 3 parts of composite fungicide, wherein the composite fungicide is prepared from the following raw materials in parts by weight: 1 to 3 parts of low-temperature cellulase-producing bacteria solution, 1 to 2 parts of normal-temperature cellulase-producing bacteria solution, 1 to 2 parts of normal-temperature amylomyces-producing bacteria solution, 2 to 4 parts of normal-temperature acetic bacteria-producing solution, 1 to 3 parts of normal-temperature yeast bacteria solution, and 3 to 5 parts of enzyme activator. The fungicide can accelerate the rapid fermentation of straws under a low-temperature condition, and the microorganisms of the fungicide have good synergistic reaction to have high-efficiency degradation performance for the straw which is difficult to degrade.

The invention discloses a rapid library building method for identifying a target protein chromatin binding map, which comprises the following steps: collecting sample cells, adding activated magneticbeads into the cells, incubating, and incubating the magnetic beads with a primary antibody; pre-binding a secondary antibody with recombinant fusion transposase; incubating the magnetic beads with the pre-combined secondary antibody and recombinant fusion transposase, adding a transposase activator and a cell perforating agent into the magnetic beads, and incubating; and terminating the transposase reaction, and carrying out library amplification. According to the method, the process of a traditional method is remarkably simplified, the library building time is shortened, the library buildingefficiency is improved, the loss is small, and the requirement for library building materials is lower. Meanwhile, three DNA standard substances with different concentrations are doped into the library building process, so that the effect of accurately quantifying the DNA copy number in the library can be achieved. The method is very suitable for researching a small amount of precious samples orsamples with different treatment states.

The invention provides an extraction method of small molecule liver peptide. The extraction method comprises following steps: step one, animal liver is mashed, purified water is added, a mixed liquidis stirred uniformly and heated to 54-58 DEG C, lipase and neutral protease are added, stirring enzymolysis is performed for 25-35 min, and a first enzymatic hydrolysate is obtained; step two, flavourzyme, chicken intestinal digestive tract protease and an enzyme activator are added to the first enzymatic hydrolysate and stirred uniformly, a mixture is cooled to 15-20 DEG C and subjected to enzymolysis extraction 2-3 times, enzymolysis time is 1-1.5 h each time, all extracting solutions are combined, and a second enzymatic hydrolysate is obtained; step three, the second enzymatic hydrolysate is heated and subjected to enzyme inactivation, a product is separated, purified, concentrated and lyophilized, and lyophilized small molecule liver peptide powder is obtained. The prepared liver peptide product is high in purity and can be widely applied to the fields of food, healthcare products, drugs and the like, and content of peptide with molecular weight being 180-500 Dalton can reach 99% or higher.

The invention discloses a method for refining tea oil for cosmetics, and relates to the field of technologies for processing oil. The method for refining the tea oil includes five steps of degumming, deacidifying, decoloring, deodorizing and fractionating. Enzyme activators such as calcium dihydrogen phosphate, potassium chloride and ferrous sulfate are added at the step of degumming and are combined with ultrasonic treatment to assist in degumming; decoloring is carried out by the aid of modified activated carbon and activated clay jointly at the step of decoloring. The method for refining the tea oil has the advantages that degumming and decoloring processes are further optimized, so that the degumming time can be shortened, the quality of decolored oil can be improved, the tea oil obtained by the aid of the method is colorless and odorless and is high in stability, oxidation and color reversion of the tea oil can be prevented, and requirements on oil for the cosmetics can be met.

Peptides that increase melanin synthesis are provided. These peptides include pentapeptides YSSWY, YRSRK, and their variants. The peptides may activate the enzymatic activity of tyrosinase to increase melanin synthesis. The pharmaceutical, cosmetic, and other compositions including the peptides are also provided. The methods of increasing melanin production in epidermis of a subject are provided where the methods include administering compositions comprising an amount of one or more peptides effective to increase the melanin production. The methods also include treating vitiligo or other hypopigmentation disorders with compositions including one or more peptides.

The invention relates to zymolytic humic acid for agriculture and a production method thereof and belongs to a humic acid and its production method. The production method solves the technical problem that the existing humic acid production method has poor activation effects and complex activation processes, produces strong corrosion to raw materials and has high energy consumption, a high cost and large environmental pollution. The zymolytic humic acid for agriculture is prepared from 100 parts by weight of weathered coal, 0.01-0.05 parts by weight of a compound enzyme activator and 15 parts by weight of an auxiliary agent. The production method comprises 1, preparing a compound enzyme activator, 2, preparing an auxiliary agent, and 3, preparing zymolytic humic acid through adjusting water content of 100 parts by weight of weathered coal to obtain coal paste with water content of 60-70%, adjusting pH through calcium hydrate, adding the compound enzyme activator into the coal paste, carrying out uniform mixing, conveying the mixture into a degradation tank, adding the auxiliary agent into the mixture, carrying out degradation, and crushing the mixture through a chain type pulverizer to obtain a zymolytic humic acid finished product.

The invention provides an extraction method of active ingredients of medicinal fungi. The extraction method comprises the following steps: (1) crushing raw materials of the medicinal fungi, wherein the diameters are 75-300 microns after the raw materials are crushed; (2) adding mixed enzymes and an enzyme activator into the crushed raw materials of the medicinal fungi to implement enzymolysis, wherein the dosage of the mixed enzymes is 1-3% of the raw materials of the medicinal fungi, the enzymolysis temperature is 20-45 DEG C, the pH value is 4-9, and enzymolysis reaction is carried out for 40-100 minutes; and (3) filtering the product subjected to enzymolysis and collecting extraction liquid to obtain the active ingredients of medicinal fungi. According to the extraction method provided by the invention, the yield is high, the energy consumption is low and the damage on the active ingredients of the medicinal fungi is alleviated, and the extraction time is remarkably shortened compared with that of the existing extraction method, and the content of triterpene and adenosine in the extract is improved to a greater extent compared with that of the existing extraction method, moreover, the operation is simple and the cost is relatively low.

The present invention discloses a wound healer for trees and an application thereof. The healer is a special preparation compounded by a plant growth regulator, a plant nutrient, a plant physiologically active substance, a bactericide, an enzyme activator and a filming protective agent so as to be similar with liquid in formative layer of bark. The present invention prepares the compound scientifically according to the characteristics of the trees and main components of the liquid in the formative layer, and solves the technical problems that wound heals slowly and the trees are injected easily; the healer enables the wound (by insects, diseases and machines) to heal and recover rapidly and form a new bark layer, can improve resistance of the trees, restores transportation of photosynthetic products in leaves and growth and vigor of the trees rapidly, achieves 100% of healing rate of the wound, and has a significant effect. Due to the fact that the healer is full of a variety of nutritional elements and is in a chelated state, the healer can be absorbed by plants quickly, and is also a micro fertilizer to promote the growth of the plant efficiently and increase yield. The product adapts injuries and disease wounds of various fruit trees and garden trees, and sprays on leaves of vegetables, fruits, legumes and other green plants of agricultural crops.

The invention discloses spinosyn A and a derivative thereof as an activator of argininosuccinate synthetase 1 (ASS1) and an activator of mutant argininosuccinate synthetase ASS1G362V and application of the Spinosyn A and the derivative thereof. The spinosyn derivative has a structural general formula (I). The spinosyn and the derivative thereof are used as a medicine and an anti-tumor medicine fortreating I-type citrullinemia by activating the argininosuccinate synthetase 1 (ASS1) and the mutant argininosuccinate synthetase ASS1G362V in a targeted manner.

The invention discloses a bio-enzyme-based efficient water purifying agent. The water purifying agent is prepared from the following components in parts by weight: 30-35 parts of bio-enzyme, 10-12 parts of microorganism, 5-8 parts of microbial flocculant, 5-8 parts of moringa seed flocculant, 3-5 parts of bio-enzyme preparation, 10-12 parts of chitin, 3-5 parts of chitosan, 5-8 parts of sodium carboxymethyl starch, 1-3 parts of functional oligopeptide, 5-8 parts of white polyaluminum chloride, 20-25 parts of water, and 20-25 parts of filler. The bio-enzyme-based efficient water purifying agent has the beneficial effects that the functional oligopeptide can be combined with enzyme so as to serve as an enzyme activator, thereby improving enzyme activity, allowing enzyme to degrade a substrate faster, and remarkably improving the water purification efficiency of the water purifying agent; and a small amount of water purifying agent can remove a wide range of impurities in to-be-purified water, the water purifying agent has high purification efficiency, and leaves no residual toxic or subsidiary substance, therefore, the bio-enzyme-based efficient water purifying agent has high economic and social benefits.

The invention belongs to the technical field of high polymer materials and water treatment, and discloses an MBBR (Moving Bed Biofilm Reactor) filler which comprises the following components: a hydrophilic substrate, a toughening agent, light calcium carbonate, a density regulator, biological nutritive salt, a biological active agent and a biological enzyme activator. The toughening agent is copolymer grafted maleic anhydride, and meanwhile, hollow glass beads are selected as the density regulator, so that the problems of high density of inorganic filler of a hydrophilic filler agent and interface between the inorganic filler and a base material are solved. The invention also discloses a preparation method of the MBBR filler. Compared with polyethylene fillers, the prepared MBBR filler hasbetter hydrophilicity and biocompatibility. When the MBBR filler is used in an MBBR process, the COD removal rate is more than 85%, the ammonia nitrogen removal rate is more than 95% and the effluentCOD is less than 30mg / L. The MBBR filler is suitable for treatment of various sewage with different carbon / nitrogen and ammonia / nitrogen ratios, and is high in efficiency, high in aging and strong inuniversality.

The invention discloses a maifanite-containing double-effect multi-component organic / inorganic compound mixed fertilizer and a manufacturing method thereof. The maifanite-containing double-effect multi-component organic / inorganic compound mixed fertilizer is low-carbon and environment-friendly and produced by organic combination of natural components including humic acid, maifanite, bentonite, potassium oxide, ammonium phosphate, ammonium chloride, borax, zinc sulfate, aluminum potassium sulfate, and traditional Chinese medicines for agricultural use (including aloe, Tripterygium wilfordii, onion juice, tobacco stem, Radix Glycyrrhizae, Aconitum kusnezoffii and Pyreyhrum), biological enzyme activators and a PASP plant growth promoter at normal temperature and pressure. The manufacturing method comprises the following steps: grinding multiple raw materials in the formula to powder of about 120 to 200 meshes, thoroughly mixing according to the given ratio in a stirring machine, conveying to a granulating machine through a conveyor, chelating by spraying a PASP aqueous solution and curing and drying at a high temperature by use of a drying machine, sieving to obtain the fertilizer product which has a diameter of 2.5 to 4.5 mm, a strength of 5 to 6 n and a free moisture of less than 10%, and quantitatively bagging after the product passes the quality test. The fertilizer product has complete nutrients and balanced nutrition, is rich in organic matters and amino acids, can activate and utilize residual fertilizers in the soil, improves yield and quality of crops, produces green and organic agricultural products, helps increase farmer's incomes, well solves the problems and difficulties caused by long-term use of chemical fertilizers in agricultural production and farming, overcomes the defects of chemical fertilizers, and truly meets the national industrial policy requirements for economic and social benefits of green, organic and environment-friendly sustained- / controlled-release fertilizers.