Method for preparing fish collagen peptides by enzymatic hydrolysis after biological fermentation, products and applications

Through the optimization of biological fermentation and enzymatic hydrolysis processes, combined with plant lactobacillus and specific enzymatic hydrolysates, the problems of insufficient quality and antibacterial effect of fish collagen peptides were solved, and high-quality fish collagen peptides were prepared for improving skin condition.

CN118598981BActive Publication Date: 2025-09-23HANCHEN (SHENYANG) KID PROD CO LTD
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Patent Information

Application Number
CN202410649396.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-05-23
Publication Date
2025-09-23
Estimated Expiration
2044-05-23

AI Technical Summary

Technical Problem

Existing methods for preparing fish collagen peptides result in lower quality and poor antibacterial properties, failing to fully utilize the added value of fish processing waste.

Method used

The enzymatic hydrolysis method after biological fermentation is adopted, using plant lactobacillus strains and specific enzymatic hydrolysates (such as papain and flavor protease) combined with enzyme activators (such as wood ear polysaccharides, oligomannose and raffinose) to optimize the enzymatic hydrolysis process to improve the quality and antibacterial effect of fish collagen peptides.

Benefits of technology

The prepared fish collagen peptide has high hydroxyproline and total nitrogen content, low ash content, significantly improves skin condition, has good antibacterial properties, and is suitable for cosmetics and medicines.

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Abstract

The present invention discloses a method for preparing fish collagen peptides by enzymatic hydrolysis after biological fermentation, and relates to the field of biotechnology. The preparation method of the fish collagen peptide comprises the following steps: (1) adding an extractant to fish tissue for extraction, filtering, and obtaining a filtrate; (2) adding a carbon source to the filtrate to obtain a fermentation medium; (3) inoculating a plant lactobacillus strain on a seed culture medium, culturing on a shaking table, and obtaining a seed liquid; (4) inoculating the seed liquid into a fermentation medium, and fermenting and culturing; (5) first adding an enzyme activator, then adding an enzymolysis agent for enzymatic hydrolysis, inactivating the enzyme, centrifuging, and drying to obtain fish collagen peptide powder; the enzyme activator described in step (5) is a fungus polysaccharide, oligomannose, and raffinose. The preparation method of the present invention ensures that the prepared fish collagen peptide has high quality and good antibacterial effect, can effectively improve symptoms such as skin sagging, dull color, greasy, and freckles, and has good application prospects.
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