Separation, extraction and purification method for cornus officinalis polysaccharides

Abstract

The invention discloses a separation, extraction and purification method for cornus officinalis polysaccharides. The method includes the following steps that a) dry cornus officinalis materials are smashed into cornus-officinalis ultrafine powder materials; b) the cornus-officinalis ultrafine powder materials are subjected to ultrasonic and enzymolysis in a cultivating tank, distilled water is added, and cornus-officinalis-polysaccharide extracting liquid is obtained; c) the cornus-officinalis-polysaccharide extracting liquid is subjected to enzyme killing treatment; d) ultrasonic, filtration,standing by adding ethyl alcohol, suction filtration and drying are carried out, and a cornus-officinalis-polysaccharide crude product is obtained; e) the cornus-officinalis-polysaccharide crude product is subjected to protein removing in a classified mode; f) the obtained product is dissolved into distilled water, centrifugal separation is carried out, supernate is taken and subjected to a DEAE-cellulose column and a chromatographic column; g) vacuum low-temperature sublimation is carried out with an eluant, and white cornus officinalis polysaccharides are obtained. According to the separation, extraction and purification method, enzymolysis, protein removing in a classified mode and distilled water chromatography are carried out in the compound enzyme spray environment, extracting timeis short, the extracting efficiency is high, the product purity of the cornus officinalis polysaccharides is high, and the industrial and experimental requirements are met.

Description

technical field [0001] The invention relates to the technical field of extracting plant polysaccharides, in particular to a method for separating, extracting and purifying polysaccharides from Cornus officinalis. Background technique [0002] Cornus officinalis is the dried and ripe pulp of Cornus officinalis, also known as medicinal jujube or jujube. It has the pharmacological effects of enhancing the immune system, anti-cancer, diuresis, blood pressure and anti-oxidation. It is a kind of nourishing and precious medicinal material. In addition, it is also widely used in the food industry. Cornus officinalis contains active ingredients such as cornus officinalis polysaccharides, organic acids, tannins, etc. Among them, cornus officinalis polysaccharides especially have immune regulation, anti-aging and other effects, and its prevention and treatment of other diseases of the human body are also getting better and better as people's understanding of it deepens. more and more ...

AI Technical Summary

Problems solved by technology

[0003] The existing polysaccharide extraction methods of Cornus officinalis are generally carried out by alcohol precipitation and filtration, followed by single deproteinization and chromatographic column, etc., but this method has low extraction efficiency, long extraction time period and low purity of polysaccharides, which is difficult to meet Disadvantages such as industrial or experimental requirements