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A kind of phytochelatin microbial bacterial agent and preparation method thereof

A technology of microbial inoculants and phytochelating peptides, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of short effective storage time, low effective viable bacteria content, and unsatisfactory effects, and achieve Good stability of microbial activity, reduced incidence of pests and diseases, and improved microbial environment

Active Publication Date: 2018-01-23
中菌控股集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In short, the existing microbial agents have the following defects: (1) low content of effective viable bacteria and high rate of miscellaneous bacteria; (2) not resistant to storage, effective storage time is short; (3) unsatisfactory effect after application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 A kind of phytochelatin microbial bacterial agent

[0031] The raw material of the microbial inoculum includes the following components by weight:

[0032] 7.9 parts of plant active peptides, 8 parts of Bacillus natto bacteria liquid, 6 parts of Pichia pastoris bacteria liquid, 3 parts of Lactobacillus casei bacteria liquid, 5.5 parts of Buttercup vine extract, 41 parts of Ulva powder, 24 parts of gallnut powder, sulphurous acid 3 parts of iron, 20 parts of potassium fulvic acid, 19 parts of chelating agent;

[0033] Described bacillus natto bacterial liquid: the content of bacillus natto is 40×10 9 CPU / ml;

[0034] Described Pichia yeast liquid: the content of Pichia pastoris is 16×10 9 CPU / ml;

[0035] Described Lactobacillus casei bacteria liquid: the content of Lactobacillus casei is 22×10 9 CPU / ml;

[0036] The chelating agent is a mixture of EDTA and superphosphate in a weight ratio of 3.5:1.

Embodiment 2

[0037] Embodiment 2 A kind of phytochelatin microbial bacterial agent

[0038] The raw material of the microbial inoculum includes the following components by weight:

[0039] 8.5 parts of plant active peptides, 10 parts of Bacillus natto bacteria liquid, 8 parts of Pichia pastoris bacteria liquid, 5 parts of Lactobacillus casei bacteria liquid, 7.2 parts of Ding Gong vine extract, 43 parts of Ulva powder, 26 parts of gallnut powder, sulfite 3.8 parts of iron, 26 parts of potassium fulvic acid, 21 parts of chelating agent;

[0040] Described bacillus natto bacterial liquid: the content of bacillus natto is 40×10 9 CPU / ml;

[0041] Described Pichia yeast liquid: the content of Pichia pastoris is 16×10 9 CPU / ml;

[0042] Described Lactobacillus casei bacteria liquid: the content of Lactobacillus casei is 22×10 9 CPU / ml;

[0043] The chelating agent is a mixture of EDTA and superphosphate in a weight ratio of 3.5:1.

Embodiment 3

[0044] Embodiment 3 A kind of phytochelatin microbial bacterial agent

[0045] The raw material of the microbial inoculum includes the following components by weight:

[0046] 8.9 parts of plant active peptides, 12 parts of Bacillus natto bacteria liquid, 10 parts of Pichia pastoris bacteria liquid, 7 parts of Lactobacillus casei bacteria liquid, 8.5 parts of Ding Gong vine extract, 45 parts of Ulva powder, 29 parts of gallnut powder, sulfite 4.5 parts of iron, 30 parts of potassium fulvic acid, 23 parts of chelating agent;

[0047] Described bacillus natto bacterial liquid: the content of bacillus natto is 40×10 9 CPU / ml;

[0048] Described Pichia yeast liquid: the content of Pichia pastoris is 16×10 9 CPU / ml;

[0049] Described Lactobacillus casei bacteria liquid: the content of Lactobacillus casei is 22×10 9 CPU / ml;

[0050] The chelating agent is a mixture of EDTA and superphosphate in a weight ratio of 3.5:1.

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Abstract

The invention provides a phytochelatin microbial agent and a preparation method thereof. The microbial agent comprises plant active peptides, bacillus natto bacterial liquid, pichia pastoris bacterial liquid, lactobacillus casei bacterial liquid, erycibe obtusifolia benth extract, ulva powder, gallnut powder, ferrous sulfate, potassium fulvic acid and a chelating agent component. In the bacillus natto bacterial liquid, the content of bacillus natto is 40*10<9>CPU / ml; in the pichia pastoris bacterial liquid, the content of pichia pastoris is 16*10<9>CPU / ml; in the lactobacillus casei bacterial liquid, the content of lactobacillus casei is 22*10<9>CPU / ml; in the chelating agent is a mixture consisting of EDTA and calcium superphosphate at a weight ratio of 3.5:1. The content of functional bacterium in the microbial agent is (4.8 to 5.2)*10<9> / g, the content of trace elements is 42 to 45 mg / g, and the infectious microbe rate is 4.5 to 5.2 percent.

Description

technical field [0001] The invention belongs to the field of microbial fertilizers, and relates to a plant chelating peptide microbial bacterial agent and a preparation method thereof. Background technique [0002] Microbial agent is a new type of biological disease-resistant bacterial fertilizer developed by using high-tech biological technology for the expansion of crop cultivation and seedling transfer area, the increase of continuous cropping and heavy cropping, and the increasingly serious crop diseases and insect pests. [0003] In the process of reproduction and metabolism, beneficial microorganisms can secrete active substances such as cytokinins, indole acetic acid, vitamins, benzyl acetic acid, gibberellin and other auxins and amino acids, which strongly promote root growth and rooting, and increase the number of strong roots and capillary roots more than double. Water absorption and fertilizer absorption ability are enhanced, the stem is thick, the seedling is st...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00C12N1/20C12N1/16C12R1/07C12R1/84C12R1/245
Inventor 刘兴旺刘玉贞刘志远
Owner 中菌控股集团有限公司
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