47results about How to "Low bacteria rate" patented technology

Belonging to the technical fields of biology and food, the invention relates to a preparation method of an active lactobacillus pickled vegetable. The method provided in the invention includes the technological steps of: A. loading a pickled vegetable liquor into a jar; B. filling the jar with vegetables; C. conducting fermentation at a constant temperature of 12-18DEG C; D. taking the fermented vegetables out of the jar, and carrying out blending and packaging. After a finished product is obtained by subjecting the pickled vegetable liquor to first round pickling, a direct vat set lactobacillus starter activating solution prepared from a direct vat set lactobacillus starter and glucose is added into the pickled vegetable liquor in the jar, and they are mixed uniformly for a second round of circulating pickled vegetable preparation. By adopting a reasonable vegetable proportion and a good flavor chilli vegetable fermented pickling liquor, the method provided in the invention well keeps the inherent flavor of the vegetable and the fermented flavor, and realizes standardized preparation of the active lactobacillus pickled vegetable, enhances the quality and homogeneity of the product, substantially improves the biosecurity of the product, and achieves a best flavor and a most suitable taste, thus further realizing upgrading and industrialized, large scale and standardized production of traditional active lactobacillus pickled vegetables.

The invention belongs to the technical field of edible fungi cultivation, and particularly relates to a planting method for selenium-enriched black fungi. The method comprises the steps of compost configuration, bagging, sterilization, inoculation, stacking, puncturing and harvesting. According to the planting method for selenium-enriched black fungi, the growth cycle of the black fungi can be shortened by 8-15 d, economic benefits are remarkably improved, meanwhile, the yield of the black fungi is increased, the percentage of contaminating microorganism and the insect attack rate are greatly lowered, and it is detected that the selenium content of the planted black fungi is 6.72-6.89 mg/kg.

The invention relates to a microbial agent. The agent contains trichoderma asperellum WL, bacillus stearothermophilus viable spores and scopulariopsis acremonium. Organic wastes are composted by usingthe composite microbial agent, so that the material decomposition degree is improved, and the agent has the complexing action on heavy metal ions for changing the states of the heavy metal ions and also has excellent tolerance. The invention further relates to an organic waste desertified soilremediation agent. The organic waste desertified soil remediation agent is prepared by mixing a fermentation product and an organic waste preparation, wherein the fermentation product is prepared by fermenting the microbial agent and the organic wastes and auxiliary materials; and the preparation methodof the organic waste preparation comprises the following steps: removing impurities, crushing, soaking with water for 10-14 hours, stirring into paste in an environment with the pH value of 9-10, andadding a vinyl monomer to carry out a graft copolymerization reaction. The soil remediation agent solves the desertification problem, and is capable of remediating heavy metal contaminated soil.

The invention provides a phytochelatin microbial agent and a preparation method thereof. The microbial agent comprises plant active peptides, bacillus natto bacterial liquid, pichia pastoris bacterial liquid, lactobacillus casei bacterial liquid, erycibe obtusifolia benth extract, ulva powder, gallnut powder, ferrous sulfate, potassium fulvic acid and a chelating agent component. In the bacillus natto bacterial liquid, the content of bacillus natto is 40*10<9>CPU/ml; in the pichia pastoris bacterial liquid, the content of pichia pastoris is 16*10<9>CPU/ml; in the lactobacillus casei bacterial liquid, the content of lactobacillus casei is 22*10<9>CPU/ml; in the chelating agent is a mixture consisting of EDTA and calcium superphosphate at a weight ratio of 3.5:1. The content of functional bacterium in the microbial agent is (4.8 to 5.2)*10<9>/g, the content of trace elements is 42 to 45 mg/g, and the infectious microbe rate is 4.5 to 5.2 percent.

The invention belongs to the technical field of preparation methods of microbial agents, particularly relates to a preparation method and application of a microbial agent adopting nano-bubble for activation and expanding culture, and solves the problem that in the prior art, after expanding culture of EM stock seeds, the number of colonies is limited, and the effective viable bacterium rate is limited and the problem that compared with the original microbial system in nature, there is no obvious advantage on bioactivity of the microbial system, and easy dissimilation and high infectious microbe rate are caused after application. The preparation method of the microbial agent adopting nano-bubbles for activation and expanding culture includes the following steps that sterile brown sugar water, honey and strain liquid are jointly added into a first-stage fermentation tank, and fermentation is carried out through combination of an expanding culture process and a micro-nano bubble generation device; and the activated strain liquid, the sterile water and the sterile brown sugar water are jointly poured into a secondary fermentation tank, and fermentation is carried out through combination of an expanding culture process and a micro-nano bubble generation device. The effective viable bacterium rate of the EM stock compound bacterial colony group is greatly improved, the purity is higher, and the infectious microbe rate is reduced to 0.1% or below.

The invention discloses a simulation black fungus section cultivation method. A simulation black fungus section includes a phloem portion 1 and a xylem portion, and is characterized in that: the phloem portion 1 is made of a plastic tube, wherein the plastic tube is made of a plastic material, the thickness of the plastic tube wall is 0.2 cm-0.5 cm, the diameter of the plastic tube wall is 5 cm-11 cm, and the phloem portion 1 serves as the outer skin (bark) of the simulation black fungus section; the xylem portion 2 serves straw and wood processing remainders as raw materials, all the raw materials are smashed and sieved, and the medium of the xylem portion is prepared according to a specified carbon nitrogen ratio and a pH, water is added into the medium, and the medium with water is stirred evenly; a special compression filling machine is used to perform tube filling to obtained a trunk-like black fungus section, wherein the diameter of the simulation black fungus section is 5 cm-11 cm, the length of the simulation black fungus section is 100 cm-120 cm; and holes is drilled, and then the black fungus can be planted in the holes.

The invention discloses a marine microbial agent and a preparation method thereof. The marine microbial agent comprises a sagittula stellata SCSIO 43504 agent and bacillus sp. SCSIO 43505 agent. The marine microbial agent disclosed by the invention is high in living bacteria amount, low in foreign bacteria amount and low in fecal coliform amount and can reach the national standard (GB 20287-2006) of the agricultural microbial agent. The marine microbial agent can precipitate around seaweeds in seawater so as to be beneficial to planting strains at the root parts of the seaweeds and playing a role in promoting the growth of the seaweeds.

The present invention relates to a preparation method of a yeast growing rapidly and having special aroma, and belongs to the field of micro-organism products. The yeast is saccharomyces sp. yeast 7#and has a preservation number of GDMCC No:60496. The yeast has a growth cycle of 65-68 hours, shortens a culture cycle compared with traditional 5-7 days and is more likely to grow and reproduce at aconstant temperature of 30 DEG C in a high humidity environment than other microorganisms of bacteria, molds, etc., and the shortening of the cycle is lowering possibility of contamination. Mycelia ofaroma-producing yeasts grow obviously after 40-48 hours of cultivation, then colony advantage is produced, and chance of the contamination is thus reduces. The mycelia of the yeasts can grow obviously in 24 hours and a growth rate is increased by 2 times.

The invention discloses a method for culturing photosynthetic bacteria by using membrane concentrated biogas slurry. The method comprises the following steps: detecting the content and pH value of nitrogen, phosphorus and potassium in the membrane concentration biogas slurry, diluting the concentrated biogas slurry according to the content of nitrogen, phosphorus and potassium, directly culturing photosynthetic bacteria by using the diluted membrane concentration biogas slurry as a culture solution, controlling the inoculation amount to be 10% of the volume of the culture solution, after inoculation, performing placing under the illumination intensity of 3000-5000Lux at the temperature of 30 DEG C, and performing culturing for 5-7 days. The membrane concentration biogas slurry is adopted as a culture solution of the photosynthetic bacteria, nutrient substances are richer after the biogas slurry is concentrated, nutrient substances do not need to be manually added any more, the dilution ratio of the concentrated biogas slurry is determined by detecting various indexes such as the nitrogen, phosphorus and potassium salt degree of the concentrated biogas slurry, and it is ensured that the concentrated biogas slurry meets the growth requirement of the photosynthetic bacteria. Most of microorganisms and all solid particles in the biogas slurry subjected to membrane concentration are removed.

The invention discloses a preparation method of high-activity mucor koji. The preparation method of the high-activity mucor koji comprises the following steps: activating strains, preparing first-level strains, preparing second-level strains, preparing third-level strains, preparing finished koji, and drying the finished koji. The invention provides the preparation method of the high-activity mucor koji. According to the preparation method of the high-activity mucor koji, with combination of solid state fermentation and pure liquid state fermentation, growth of mixed bacteria can be effectively inhibited, so that the produced mucor koji has the advantages of high activity, low mixed bacteria rate and stable performance.

The invention discloses a culture medium special for Fritillaria thunbergii and a preparation process and system of the culture medium. The culture medium and the preparation process and system have the advantages that bamboo carbon and refined bamboo vinegar serving as the main raw materials are compounded to obtain the culture medium special for the Fritillaria thunbergii, and the culture mediumcan be directly applied to the seedling culture of the Fritillaria thunbergii and can also be directly applied in the field to serve as the medium accompanying fertilizer; by the culture medium, maximized compounding of nutrition needed by the propagation of Fritillaria thunbergii bulbs is achieved, maximized utilization of key nutrition is guaranteed, and Fritillaria thunbergii vegetative propagation emergence rate can be increased.

The invention relates to a method used for preparing an algae particle potassium fertilizer via compound microorganism fermentation. The method comprises following steps: fresh marine algae plants are subjected to washing, impurity removing, immersing, and pulping so as to prepare seed fermentation broth of fermentative strain respectively; an algae base material is inoculated with the seed fermentation broth in a plurality of times so as to realize fractional fermentation; a fermentation product is prepared into an algae functional microbial inoculum further; mild impact pelletizing is adopted to realize combination the algae functional microbial inoculum with chemical fertilizer raw materials so as to obtain the algae synergistic-effect potassium fertilizer particle rich in algae extract and special-effect composite fermentative strain. It is shown by experiment that the technical effect of the compound fractional fermentation is better than that of single strain fermentation, and the fertilizer effect of the algae functional potassium fertilizer is obviously better than that of conventional potassium fertilizer products.

The invention discloses a preparation process of bacillus licheniformis. The method comprises seed culture and fermentation culture, wherein the seed culture comprises liquefaction and sterilization;the fermentation tank culture comprises inoculation and fermentation culture; the process steps are rigorous; through detailed description of each step and solidification of environmental parameters in each step, the occurrence probability of maldevelopment of bacillus in the fermentation cultivation process is reduced; the bacillus licheniformis fermentation technology guarantees that the numberand the quality of the fermented bacillus licheniformis are far higher than those of fermentation results in the prior art, the environment in the preparation process of the technology is a sterile environment, the infectious microbe rate in the produced product is low, the product quality is high, and the bacillus licheniformis prepared through the technology is long in quality guarantee period and can be stored for a long time.