47results about How to "Low bacteria rate" patented technology

Belonging to the technical fields of biology and food, the invention relates to a preparation method of an active lactobacillus pickled vegetable. The method provided in the invention includes the technological steps of: A. loading a pickled vegetable liquor into a jar; B. filling the jar with vegetables; C. conducting fermentation at a constant temperature of 12-18DEG C; D. taking the fermented vegetables out of the jar, and carrying out blending and packaging. After a finished product is obtained by subjecting the pickled vegetable liquor to first round pickling, a direct vat set lactobacillus starter activating solution prepared from a direct vat set lactobacillus starter and glucose is added into the pickled vegetable liquor in the jar, and they are mixed uniformly for a second round of circulating pickled vegetable preparation. By adopting a reasonable vegetable proportion and a good flavor chilli vegetable fermented pickling liquor, the method provided in the invention well keeps the inherent flavor of the vegetable and the fermented flavor, and realizes standardized preparation of the active lactobacillus pickled vegetable, enhances the quality and homogeneity of the product, substantially improves the biosecurity of the product, and achieves a best flavor and a most suitable taste, thus further realizing upgrading and industrialized, large scale and standardized production of traditional active lactobacillus pickled vegetables.

The invention belongs to the technical field of edible fungi cultivation, and particularly relates to a planting method for selenium-enriched black fungi. The method comprises the steps of compost configuration, bagging, sterilization, inoculation, stacking, puncturing and harvesting. According to the planting method for selenium-enriched black fungi, the growth cycle of the black fungi can be shortened by 8-15 d, economic benefits are remarkably improved, meanwhile, the yield of the black fungi is increased, the percentage of contaminating microorganism and the insect attack rate are greatly lowered, and it is detected that the selenium content of the planted black fungi is 6.72-6.89 mg / kg.

The invention provides a microbial agent and a preparing method thereof. The microbial agent is prepared from jelly type bacillus, bacillus subtilis and bacillus licheniformis. In the microbial agent, the viable count of jelly type bacillus ranges from 1.8 billon cfu / g to 2.2 billon cfu / g, the viable count of bacillus subtilis ranges from 1.7 billon cfu / g to 1.9 billon cfu / g, and the viable count of bacillus licheniformis ranges from 1.2 billon cfu / g to 1.3 billon cfu / g. The preparing method of the microbial agent comprises the steps of strain selection, strain activation and expanding culture, strain fermentation and finished product obtaining. The microbial agent is high in strain content, low in infectious microbe rate and capable of effectively improving soil properties.

The invention relates to the biotechnical field, and in particular relates to a method for producing bacillus laterosporus live bacteria by high density solid fermentation. The method comprises the following steps: culture selection; bevel seed cultivation; primary solid seed cultivation; secondary solid seed cultivation; solid fermentation and cultivation; fermentation product drying; and fermentation product flash evaporation. Raw materials of the culture media provided by the invention are subsidiary agricultural products which are low in cost and reasonable in formula and can meet the nutritional demand of strain reproduction. The pH value of the culture media in the process of cultivation by adding a calcium carbonate buffer is in particularly changed, so that the content of active bacteria of bacillus laterosporus by solid fermentation is greatly improved. Experiments and production show that the number of effective active bacteria of bacillus laterosporus prepared by the method can reach 50 billion / g, the percentage of contaminating microorganisms of the product is lower than 5% and the spore rate reaches over 90%, so that the production coast of enterprises is greatly lowered, and the demand of production of microbial agents is satisfied.

The invention relates to a microbial agent. The agent contains trichoderma asperellum WL, bacillus stearothermophilus viable spores and scopulariopsis acremonium. Organic wastes are composted by usingthe composite microbial agent, so that the material decomposition degree is improved, and the agent has the complexing action on heavy metal ions for changing the states of the heavy metal ions and also has excellent tolerance. The invention further relates to an organic waste desertified soilremediation agent. The organic waste desertified soil remediation agent is prepared by mixing a fermentation product and an organic waste preparation, wherein the fermentation product is prepared by fermenting the microbial agent and the organic wastes and auxiliary materials; and the preparation methodof the organic waste preparation comprises the following steps: removing impurities, crushing, soaking with water for 10-14 hours, stirring into paste in an environment with the pH value of 9-10, andadding a vinyl monomer to carry out a graft copolymerization reaction. The soil remediation agent solves the desertification problem, and is capable of remediating heavy metal contaminated soil.

The invention provides a streptomyces microflavus solid fermentation medium and a preparation method thereof, a streptomyces microflavus fermentation method and fermentation product, bio-control products of the fermentation product as well as application of the fermentation product and bio-control products, and relates to the technical field of microbial fermentation. The streptomyces microflavussolid fermentation medium is prepared from the following components in parts by weight: 40-50 parts of bran, 15-25 parts of rice hull, 25-35 pats of rice bran, 3-7 parts of calcium carbonate, and water. The streptomyces microflavus fermentation method comprises the steps of optionally enabling a strain of streptomyces microflavus to be subjected to seed activation and liquid seed culture, and performing fermentation culture by using the streptomyces microflavus solid fermentation medium to obtain the fermentation product. The streptomyces microflavus fermentation method overcomes the defect that the existing streptomyces microflavus is less in viable count and sporulation quantity. When being used for solid fermentation culture, the streptomyces microflavus solid fermentation medium is short in fermentation cycle and more in viable count, thus being suitable for industrial production; the obtained fermentation product has a significant effect in the aspect of controlling bacterial wiltof crops, thus increasing the quality and yield of the crops.

The invention discloses compound bioenzyme fertilizer which comprises livestock-poultry excrement, straw, turf, potassium chloride, humic acid, calcium carbonate, sodium sulfite, various microbial agents, various bioenzyme preparations and a fermentation accelerant. Various microbial preparations are synergistic, so that the compound bioenzyme fertilizer can quickly decompose organic matters, metabolize out anti-oxidation substances, inhibit growth and propagation of harmful microorganisms, improve flora in soil and restore soil environment and has active impact on promoting crop growth.

The invention provides a method for preparing trichoderma harzianum high-activity conidial powder. The method comprises the following steps: performing step-by-step propagating fermentation on a trichoderma harzianum strain, from preparation production of stock culture, mother spawn and spawn to production of high-concentration trichoderma harzianum chlamydospore fermentation liquor by adopting astepwise liquid fermentation method; and concentrating the fermentation liquor, adding different functional additives and filler into the concentrated fermentation liquor, and performing drying treatment to obtain the trichoderma harzianum high-activity conidial powder. The trichoderma harzianum chlamydospore has the number of effective living bacteria being 2*10<9>cfu / g or more, and has the advantages of high activity, long shelf life, high germination rate, quick soil environment adaptability and the like.

The invention discloses a culture method of selenium-rich black fungus, and relates to the technical field of black fungus planting. According to the culture method of the selenium-rich black fungus, hexamethylphosphoramide is added into a culture medium and is combined with sodium selenite in the culture medium to generate organic selenium; sodium selenite inorganic selenium is converted into organic selenium, so that the absorption of the black fungus on the selenium element can be promoted; the goal of enriching the selenium enrichment by the black fungus is achieved; traditional Chinese medicine ingredients are also added in the culture medium; the effects of sterilization, mildew prevention, worm expelling, black fungus immunity improvement, growth promotion and yield increase are achieved; the normal growth of the black fungus is ensured; the survival rate is improved; the traditional Chinese medicine ingredients are plant sources, are easy to decompose, so that the pesticide residue cannot be caused; scientificalness and environment protection are realized; the health of eaters can be ensured.

The invention provides a phytochelatin microbial agent and a preparation method thereof. The microbial agent comprises plant active peptides, bacillus natto bacterial liquid, pichia pastoris bacterial liquid, lactobacillus casei bacterial liquid, erycibe obtusifolia benth extract, ulva powder, gallnut powder, ferrous sulfate, potassium fulvic acid and a chelating agent component. In the bacillus natto bacterial liquid, the content of bacillus natto is 40*10<9>CPU / ml; in the pichia pastoris bacterial liquid, the content of pichia pastoris is 16*10<9>CPU / ml; in the lactobacillus casei bacterial liquid, the content of lactobacillus casei is 22*10<9>CPU / ml; in the chelating agent is a mixture consisting of EDTA and calcium superphosphate at a weight ratio of 3.5:1. The content of functional bacterium in the microbial agent is (4.8 to 5.2)*10<9> / g, the content of trace elements is 42 to 45 mg / g, and the infectious microbe rate is 4.5 to 5.2 percent.

The invention relates to a preparation method of active lactic acid bacteria pickles, belonging to the technical field of biology and food. The process steps of the present invention include: A. kimchi water filling altar, B. vegetable filling, C. constant temperature fermentation at 12-18°C, D. dispensing and packaging out of the altar; The kimchi water in the altar is made by adding the direct-injection type lactic acid bacteria starter and the direct-injection type lactic acid bacteria starter activation solution made from glucose, and after mixing evenly, the second round of circulation kimchi production is carried out. The present invention adopts a reasonable vegetable ratio and good-flavored capsicum-vegetable fermented pickling water, which better maintains the inherent and fermented flavor of the vegetable, realizes the standardized production of active lactic acid bacteria pickles, and improves the quality and uniformity of the product , significantly improved the biological safety of the product, achieved the best flavor and the most suitable taste, and then realized the upgrading of traditional active lactic acid bacteria kimchi and industrialized, large-scale and standardized production.

The invention belongs to the technical field of preparation methods of microbial agents, particularly relates to a preparation method and application of a microbial agent adopting nano-bubble for activation and expanding culture, and solves the problem that in the prior art, after expanding culture of EM stock seeds, the number of colonies is limited, and the effective viable bacterium rate is limited and the problem that compared with the original microbial system in nature, there is no obvious advantage on bioactivity of the microbial system, and easy dissimilation and high infectious microbe rate are caused after application. The preparation method of the microbial agent adopting nano-bubbles for activation and expanding culture includes the following steps that sterile brown sugar water, honey and strain liquid are jointly added into a first-stage fermentation tank, and fermentation is carried out through combination of an expanding culture process and a micro-nano bubble generation device; and the activated strain liquid, the sterile water and the sterile brown sugar water are jointly poured into a secondary fermentation tank, and fermentation is carried out through combination of an expanding culture process and a micro-nano bubble generation device. The effective viable bacterium rate of the EM stock compound bacterial colony group is greatly improved, the purity is higher, and the infectious microbe rate is reduced to 0.1% or below.

The invention discloses a method for recycling municipal sludge. The method comprises mixing sludge, straws, YM bacteria, an amino acid stock solution, bacillus amyloliquefaciens SQR9 functional bacteria and trichoderma harzianum T37 functional fungi, conducting high temperature and low oxygen fermentation, conducting crushing, sieving and functional fermentation, and then crushing and sieving toobtain a finished product. Main raw materials are the municipal sludge and the crop straws that are large in quantity, large-scale and periodic production can be achieved, the sludge is from a local sewage treatment plant and meets the CJ / T309-2009 standard, and a raw material source is safe. The high temperature low oxygen and functional fermentation technology is utilized to solve the problem ofenvironment pollution caused by organic solid waste and achieve organic solid waste resource utilization and stable utilization.

The invention provides a preparation process of a novel magnetized three-stage fermentation organic fertilizer. The preparation process comprises a fermentation process, a decomposition and aging process, a microwave treatment process, a magnetization treatment process and material compounding. According to the preparation process, three stages of processes of decomposing and aging, microwave treatment and magnetization treatment are added for the first time on the basis of traditional aerobic fermentation, so that the treated material is richer in micromolecular organic nutrition, less in infectious microbes and harmful substances and more stable in fertilizer efficiency.

The invention discloses a simulation black fungus section cultivation method. A simulation black fungus section includes a phloem portion 1 and a xylem portion, and is characterized in that: the phloem portion 1 is made of a plastic tube, wherein the plastic tube is made of a plastic material, the thickness of the plastic tube wall is 0.2 cm-0.5 cm, the diameter of the plastic tube wall is 5 cm-11 cm, and the phloem portion 1 serves as the outer skin (bark) of the simulation black fungus section; the xylem portion 2 serves straw and wood processing remainders as raw materials, all the raw materials are smashed and sieved, and the medium of the xylem portion is prepared according to a specified carbon nitrogen ratio and a pH, water is added into the medium, and the medium with water is stirred evenly; a special compression filling machine is used to perform tube filling to obtained a trunk-like black fungus section, wherein the diameter of the simulation black fungus section is 5 cm-11 cm, the length of the simulation black fungus section is 100 cm-120 cm; and holes is drilled, and then the black fungus can be planted in the holes.

The invention provides a fermental cultivation process of bacillus licheniformis. The fermental cultivation process comprises the seed tank cultivation stage and the fermentation tank cultivation stage, specifically, the liquefying, sterilizing, inoculating and cultivating steps are included at the seed tank cultivation stage, and culture medium raw materials adopted for seed tank cultivation comprise corn flour, soybean meal, glucose, dipotassium phosphate, monopotassium phosphate, magnesium sulfate and manganese sulfate; and during fermental cultivation, the ventilation quantity in the firstfour hours is 1:(1 to 1.1), the later ventilation quantity is 1:(1.2 to 1.3), the cultivation temperature is 37+ / -0.5 DEG C, the stirring speed is 150-160 rpm in the first four hours and is 180-190 rpm after four hours, and the cultivation time is 30+ / -0.5 hours. According to the fermental cultivation process of the bacillus licheniformis, the gemma rate of the bacillus licheniformis reaches 98%or above.

The invention provides a trichoderma viride solid fermentation technology. The trichoderma viride solid fermentation technology comprises the processes of seeding tank cultivating and fermentation tank cultivating. According to the seeding tank cultivating process, the pH of a culture medium is controlled to be 5.0-5.2, the culture pressure is 0.05-0.06 Mpa, and the culture temperature is 30+ / -0.5DEG C. According to the fermentation tank cultivating process, the ventilation quantity is 1 to 1, the stirring speed is 180-190 r / s, and the culture time is 23-24 hours. According to the trichodermaviride solid fermentation technology, the yield of spore is high, and the spore number of trichoderma viride after preparation and drying is greater than or equal to 1.5*10<10> piece / g.

The invention discloses a marine microbial agent and a preparation method thereof. The marine microbial agent comprises a sagittula stellata SCSIO 43504 agent and bacillus sp. SCSIO 43505 agent. The marine microbial agent disclosed by the invention is high in living bacteria amount, low in foreign bacteria amount and low in fecal coliform amount and can reach the national standard (GB 20287-2006) of the agricultural microbial agent. The marine microbial agent can precipitate around seaweeds in seawater so as to be beneficial to planting strains at the root parts of the seaweeds and playing a role in promoting the growth of the seaweeds.

The present invention relates to a preparation method of a yeast growing rapidly and having special aroma, and belongs to the field of micro-organism products. The yeast is saccharomyces sp. yeast 7#and has a preservation number of GDMCC No:60496. The yeast has a growth cycle of 65-68 hours, shortens a culture cycle compared with traditional 5-7 days and is more likely to grow and reproduce at aconstant temperature of 30 DEG C in a high humidity environment than other microorganisms of bacteria, molds, etc., and the shortening of the cycle is lowering possibility of contamination. Mycelia ofaroma-producing yeasts grow obviously after 40-48 hours of cultivation, then colony advantage is produced, and chance of the contamination is thus reduces. The mycelia of the yeasts can grow obviously in 24 hours and a growth rate is increased by 2 times.

The invention discloses a method for culturing photosynthetic bacteria by using membrane concentrated biogas slurry. The method comprises the following steps: detecting the content and pH value of nitrogen, phosphorus and potassium in the membrane concentration biogas slurry, diluting the concentrated biogas slurry according to the content of nitrogen, phosphorus and potassium, directly culturing photosynthetic bacteria by using the diluted membrane concentration biogas slurry as a culture solution, controlling the inoculation amount to be 10% of the volume of the culture solution, after inoculation, performing placing under the illumination intensity of 3000-5000Lux at the temperature of 30 DEG C, and performing culturing for 5-7 days. The membrane concentration biogas slurry is adopted as a culture solution of the photosynthetic bacteria, nutrient substances are richer after the biogas slurry is concentrated, nutrient substances do not need to be manually added any more, the dilution ratio of the concentrated biogas slurry is determined by detecting various indexes such as the nitrogen, phosphorus and potassium salt degree of the concentrated biogas slurry, and it is ensured that the concentrated biogas slurry meets the growth requirement of the photosynthetic bacteria. Most of microorganisms and all solid particles in the biogas slurry subjected to membrane concentration are removed.

The invention discloses a preparation method of high-activity mucor koji. The preparation method of the high-activity mucor koji comprises the following steps: activating strains, preparing first-level strains, preparing second-level strains, preparing third-level strains, preparing finished koji, and drying the finished koji. The invention provides the preparation method of the high-activity mucor koji. According to the preparation method of the high-activity mucor koji, with combination of solid state fermentation and pure liquid state fermentation, growth of mixed bacteria can be effectively inhibited, so that the produced mucor koji has the advantages of high activity, low mixed bacteria rate and stable performance.

The invention discloses a culture medium special for Fritillaria thunbergii and a preparation process and system of the culture medium. The culture medium and the preparation process and system have the advantages that bamboo carbon and refined bamboo vinegar serving as the main raw materials are compounded to obtain the culture medium special for the Fritillaria thunbergii, and the culture mediumcan be directly applied to the seedling culture of the Fritillaria thunbergii and can also be directly applied in the field to serve as the medium accompanying fertilizer; by the culture medium, maximized compounding of nutrition needed by the propagation of Fritillaria thunbergii bulbs is achieved, maximized utilization of key nutrition is guaranteed, and Fritillaria thunbergii vegetative propagation emergence rate can be increased.

The invention relates to the field of microbial inoculants, and discloses a solid culture medium used for lactobacillus plantarum fermentation, a lactobacillus plantarum solid inoculant, and a preparation method for and application of the lactobacillus plantarum solid inoculant. The solid culture medium used for lactobacillus plantarum fermentation is prepared from the following raw materials: a starchy raw material crushed product, starch dregs, carbonate, yeast extract powder and saccharides. The invention further provides a preparation method for the lactobacillus plantarum solid inoculant,and the method comprises the step of inoculating a lactobacillus plantarum strain into the solid culture medium for fermentation. The lactobacillus plantarum solid inoculant is produced through a solid state fermentation method by using the solid culture medium disclosed by the invention, so that the prepared lactobacillus plantarum solid inoculant has full and strong thalli, the effective viablecount is greater than 500 hundred million cfu / g, and the mixing uniformity variable coefficient is less than 5%; The lactobacillus plantarum solid inoculant has few operation steps and low mixed bacteria rate; the product quality is promoted; equipment and energy consumption is reduced, and wastewater is greatly reduced, thereby reducing the environmental burden.

The invention relates to a method used for preparing an algae particle potassium fertilizer via compound microorganism fermentation. The method comprises following steps: fresh marine algae plants are subjected to washing, impurity removing, immersing, and pulping so as to prepare seed fermentation broth of fermentative strain respectively; an algae base material is inoculated with the seed fermentation broth in a plurality of times so as to realize fractional fermentation; a fermentation product is prepared into an algae functional microbial inoculum further; mild impact pelletizing is adopted to realize combination the algae functional microbial inoculum with chemical fertilizer raw materials so as to obtain the algae synergistic-effect potassium fertilizer particle rich in algae extract and special-effect composite fermentative strain. It is shown by experiment that the technical effect of the compound fractional fermentation is better than that of single strain fermentation, and the fertilizer effect of the algae functional potassium fertilizer is obviously better than that of conventional potassium fertilizer products.

The invention discloses a preparation process of bacillus licheniformis. The method comprises seed culture and fermentation culture, wherein the seed culture comprises liquefaction and sterilization;the fermentation tank culture comprises inoculation and fermentation culture; the process steps are rigorous; through detailed description of each step and solidification of environmental parameters in each step, the occurrence probability of maldevelopment of bacillus in the fermentation cultivation process is reduced; the bacillus licheniformis fermentation technology guarantees that the numberand the quality of the fermented bacillus licheniformis are far higher than those of fermentation results in the prior art, the environment in the preparation process of the technology is a sterile environment, the infectious microbe rate in the produced product is low, the product quality is high, and the bacillus licheniformis prepared through the technology is long in quality guarantee period and can be stored for a long time.

The invention discloses a culture medium special for Fritillaria thunbergii and a preparation process and system of the culture medium. The culture medium and the preparation process and system have the advantages that bamboo carbon and refined bamboo vinegar serving as the main raw materials are compounded to obtain the culture medium special for the Fritillaria thunbergii, and the culture mediumcan be directly applied to the seedling culture of the Fritillaria thunbergii and can also be directly applied in the field to serve as the medium accompanying fertilizer; by the culture medium, maximized compounding of nutrition needed by the propagation of Fritillaria thunbergii bulbs is achieved, maximized utilization of key nutrition is guaranteed, and Fritillaria thunbergii vegetative propagation emergence rate can be increased.

The invention provides a compounded amino-acid microbial bacterium agent and a preparation method therefor. The microbial bacterium agent is prepared from the raw material ingredients in parts by weight: 38 parts of Sphingomonas yanoikuyae powder, 12 parts of Lactobacillus bulgaricus powder, 25 parts of Azotobacter chroococcum powder, 20 parts of Bacillus laterosporus powder, 9 parts of compounded amino acid salt, 20 parts of zymosan and 26 parts of choline chloride. The compounded amino acid salt contains L-histidine salt, L-cysteine salt, L-ornithine salt and L-lysine-L-aspartate, wherein the weight content ratio of the L-histidine salt to the L-cysteine salt to the L-ornithine salt to the L-lysine-L-aspartate is 5: 3: 2: 2. The compounded amino-acid microbial bacterium agent provided by the invention has the pH value of 6.8 and the moisture content of 18-22%; the content of effective living bacteria is 6.6-7.9*10<9> / g, and the ratio of miscellaneous bacteria is 3.2-4.7%.

The invention discloses an application of Bacillus megaterium to treatment of municipal sludge and a method for producing a biological organic fertilizer from Bacillus megaterium. The preservation number of Bacillus megaterium is ACCC10010, the organism is classified and named as Bacillus megaterium, and Bacillus megaterium is preserved in Agricultural Culture Collection of China. The method is used for treating municipal sludge, sludge samples before and after fermentation are determined, organic pollutants in the sludge are basically and completely degraded, concentration of heavy metal ions is substantially reduced, and the purpose for harmless treatment of the municipal sludge is realized. The biological organic fertilizer produced from fermentation liquor which is generated in the sludge treatment process and used as a raw material is detected, the effective living bacteria number of Bacillus megaterium in a finished product is 1.76x10<8>MPN / g, mixed bacterium rate is lower than 10%, pH is 6.51, and the results accord with a biological organic fertilizer standard of department of agriculture (NY / T 884-2004).