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Method for improving tolerance of soybean to petroleum and lead contaminated soil by applying photosynthetic bacteria

A technology for lead-contaminated soil and photosynthetic bacteria, applied in the fields of botanical equipment and methods, horticultural methods, applications, etc., can solve problems such as large differences in tolerance, and achieve improved physical and chemical properties, improved resistance indicators, and environmentally friendly products. Effect

Inactive Publication Date: 2013-04-03
SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
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  • Abstract
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AI Technical Summary

Problems solved by technology

Crop tolerance to oil and lead contamination varies widely

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0020] This method is suitable for improving the resistance and yield of soybean in compound polluted soil with oil concentration lower than 1500 mg / kg and lead concentration lower than 200 mmol / L.

[0021] Before sowing soybeans, spray 200-fold diluted bacterial agents on the soil. Dilute the bacterial agent 50 times and soak the seeds before sowing. After sowing, the bacterial agent is diluted 500 times, and apply 30-50ml of the bacterial agent to each hole. During soybean growth, the soybean chlorophyll content was monitored weekly from the seedling stage. The method is: select 3-5 typical plants in the group, select the fully expanded leaves at the uppermost node, according to Chen Jianxun (Edited by Chen Jianxun, "Plant Physiology Experiment Guide (Second Edition)", South China University of Technology Press, page 87 ) method for the determination of chlorophyll content. If the chlorophyll content is around 3.0 mg / L, it means that the plant growth is relatively normal a...

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Abstract

The invention provides a method for improving tolerance of soybeans to petroleum and lead contaminated soil by applying photosynthetic bacteria, and relates to a method for improving soil tolerance. The method comprises the following steps of: before soybean seeds are sowed, spraying dilute bacterial agent to soil, diluting bacterial agent, soaking the seeds in the dilute bacterial agent and sowing the seeds; after the seeds are sowed, infiltrating dilute bacteria; and in the soybean growth process, monitoring the content of soybean chlorophyll every week from the seedling stage: selecting typical plants in a population, selecting fully-unfolded leaves at the topmost node positions, determining the content of the chlorophyll, or from the podding starting stage to the seed filling stage, respectively taking and spraying calcium chloride solution on leaf surfaces once, and then taking, diluting and spraying photosynthetic bacteria agent stock solution once. The method for improving the tolerance of the soybeans to the petroleum and lead contaminated soil by applying the photosynthetic bacteria has the advantages that the use cost is low, the process is safe, the environmental friendliness after use is good, the contents of the chlorophyll and carotenoid are increased very obviously, the plants are high, the stalks are thick, the dry weight and the fresh weight are greatly improved, the resistance indexes such as contents of proline, POD (peroxidase) and phytochelatin are improved, the content of malonaldehyde is reduced, the soybeans can tolerate the petroleum and lead contaminated soil, the physical and chemical properties of the soil can be remarkably improved and the soil fertility is improved.

Description

technical field [0001] The invention relates to a method for improving soil tolerance, in particular to a method for applying photosynthetic bacteria to improve soybean tolerance to oil and lead polluted soil. Background technique [0002] Petroleum is a complex mixture composed of various substances, mainly including saturated hydrocarbons, aromatic hydrocarbons, n-alkanes, asphaltenes, resins, etc. my country is a big country in oil production and consumption. In 1978, the annual output of crude oil exceeded 100 million tons and became one of the top ten oil producing countries in the world. However, the conditions for oil production and environmental protection technology are relatively backward. According to statistics, 600,000 tons of oil enters the environment through running, escaping, dripping, and leaking in my country every year, causing pollution to soil, groundwater, and surface water. In addition, sewage irrigation is also one of the causes of soil oil polluti...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/06
Inventor 邵双郭晓雷关丽杰李海燕
Owner SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
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