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Phytochelatin iron supplementation powder containing iron and preparation method of phytochelatin iron supplementation powder

A technology of chelating peptides and iron chelation, which is applied in the preparation methods of peptides, chemical instruments and methods, and medical preparations containing active ingredients, etc. It can solve the problems of low iron absorption utilization rate and poor stability, and achieve high iron absorption rate, improve stability, and inhibit oxidation

Inactive Publication Date: 2016-03-23
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an iron-containing chelating peptide iron-supplementing powder with iron-supplementing effect and a preparation method thereof in order to solve the problems of low iron absorption and utilization rate and poor stability of iron-supplementing products in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Preparation of iron chelating peptide: Fresh pig blood was centrifuged at 3000 rpm for 15 minutes to obtain blood cells in the lower layer, and the blood cells were homogeneously broken at 20 MPa to obtain porcine hemoglobin. Take 4 g of porcine hemoglobin, add 96 g of water, and use 0.1 g of porcine hemoglobin under the condition of pH=9.0 Alkaline protease was hydrolyzed for 2 hours, and the temperature was raised to 85°C to inactivate the enzyme for 10 minutes. The enzymolysis solution was decolorized with activated carbon for 50 minutes. After decolorization, the supernatant was dried to obtain the porcine hemoglobin polypeptide. Ferrous chloride was chelated with a mass ratio of 3:1 for 30 minutes, and the reactant was treated with 3 times the volume of ethanol solution to obtain a precipitate, which was repeatedly suction filtered until the filter residue was no longer precipitated, and freeze-dried into powder to obtain porcine hemoglobin iron chelating peptide...

Embodiment 2

[0019] (1) Preparation of iron chelating peptide: fresh pig blood was centrifuged at 3500 rpm for 15 minutes to obtain blood cells in the lower layer, and the blood cells were homogeneously broken at 30 MPa to obtain hemoglobin. Take 4 g of porcine hemoglobin, add 64 g of water, and use 0.12 g of enzyme under the condition of pH 8.5 Decolorize for 3 hours, increase the temperature to 90°C to inactivate the enzyme for 15 minutes, decolorize the enzymolysis solution with activated carbon for 60 minutes, dry the supernatant after decolorization to obtain porcine hemoglobin polypeptide, wherein the degree of hydrolysis of hemoglobin is 23.18%, and mix porcine hemoglobin polypeptide with ferrous chloride Chelate at a mass ratio of 2:1 for 35 minutes, treat the reactant with 2 times the volume of ethanol solution to obtain a precipitate, repeatedly suction filter until the filter residue is no longer precipitated, freeze-dry into powder, and obtain porcine hemoglobin iron chelating pe...

Embodiment 3

[0024] (1) Preparation of iron chelating peptide: Fresh pig blood was centrifuged at 4500 rpm for 15 minutes to obtain blood cells in the lower layer, and the blood cells were homogeneously broken at 40 MPa to obtain hemoglobin. Take 6 g of pig hemoglobin, add 70 g of water, and use 0.24 g of enzyme under the condition of pH 8.0 Decolorize for 5 hours, increase the temperature to 95°C to inactivate the enzyme for 20 minutes, decolorize the enzymolysis solution with activated carbon for 70 minutes, and dry the supernatant after decolorization to obtain porcine hemoglobin polypeptide, wherein the degree of hydrolysis of hemoglobin is 24.45%. The porcine hemoglobin polypeptide and ferrous chloride The mass ratio of 1:1 was chelated for 40 minutes, and the reactant was treated with 4 times the volume of ethanol solution to obtain a precipitate. Repeated suction filtration until the filter residue was no longer precipitated, freeze-dried into powder, and the porcine hemoglobin iron c...

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Abstract

The invention provides phytochelatin iron supplement powder which contains iron and has an iron supplementation function and a preparation method of the phytochelatin iron supplementation powder and belongs to the technical field of health-care food. The preparation method includes the steps that fresh pig blood is centrifuged, lower-layer blood cells are obtained, wall breaking is conducted on blood cell homogeneity, hemoglobin is obtained, the hemoglobin is subjected to enzymolysis with alkaline protease, after enzyme deactivation is conducted, enzymatic hydrolysate is subjected to decoloration with a decolorising agent, and liquid supernatant is dried after decoloration, and porcine hemoglobin polypeptide is obtained; chelation with ferrous chloride is conducted, reactant is treated through an ethanol solution, sediment is obtained, suction filtration is conducted repeatedly till filter residues are not separated out any more, freezing and drying are conducted, powder is obtained, and porcine hemoglobin iron phytochelatin is obtained; then 0.2-0.4 part of porcine hemoglobin iron phytochelatin , 30-50 parts of red bean powder, 20-30 parts of jujube powder, 8-10 parts of glucose and 0.3-0.5 part of glucose are fully mixed to be uniform, and the phytochelatin iron supplement powder containing iron and with the iron supplementation function is obtained. Through in-vitro simulation digestion and absorption inspection, the iron absorption rate of a product to which the porcine hemoglobin iron phytochelatin is added is 14-31% higher than that of a product to which ferrous chloride is added, a high iron absorption rate is achieved, and a good iron supplementation function can be achieved.

Description

technical field [0001] The invention belongs to the technical field of health food, and in particular relates to an iron-containing chelating peptide iron-supplementing powder with an iron-supplementing effect and a preparation method thereof. Background technique [0002] Iron is one of the essential trace elements for the human body. The iron content in the human body is about 3-5g. Participate in the synthesis of hemoglobin, myoglobin, cytochrome and certain enzymes in the human body. Iron deficiency in the body may lead to diseases such as iron deficiency anemia, diabetes, mental retardation, among which iron deficiency anemia is the most serious. Iron deficiency anemia is a global nutritional disease, and the incidence rate is higher in women, pregnant women and children. For the prevention and treatment of iron-deficiency anemia, iron-supplementing drugs are mostly used at present, and iron-supplementing foods are less. Most of the iron supplement products on the ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/30A61K38/01A61P3/02A23L33/165A23L33/18
CPCC12P21/06A61K38/017C07K1/30C07K1/34
Inventor 潘风光赵静张旭晴
Owner JILIN UNIV
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