63 results about "Iron Chelating" patented technology

The invention relates to a preparation method for iron-chelated collagen peptide of hairtail fish-bones. The preparation method specifically comprises the following steps: extracting collagen by taking hairtail fish-bones as raw materials through an acid process; performing enzymolysis on collagen by utilizing compound-flavor protease and neutral protease; and obtaining iron-chelated collagen peptide Gly-Pro-Pro-Gly-Pro-Trp-Gly-Pro-His-Gly by adopting ultrafiltration, immobilized iron ion affinity chromatography and inverted-phase efficient liquid-phase chromatographic separation and purification, wherein molecular weight detected by ESI/MS is 957.99Da. Because of unique chealting and transfer mechanisms, the iron-chelated collagen peptide is high in absorption utilization rate, and polypeptide/amino acid and iron can be supplemented simultaneously, so that the iron-chelated collagen peptide is an ideal iron supplementing substance.

The invention discloses a fulvic acid potassium-containing total-nutrient fertilizer for special purpose of potatoes and a production method thereof, and belongs to the technical field of crop formula special fertilizers. The fertilizer is prepared from the following raw materials: urea, monoammonium phosphate, monopotassium phosphate, potassium sulfate, boric acid, EDTA chelated zinc, EDTA chelated iron, EDTA chelated manganese, ammonium heptamolybdate, anhydrous magnesium sulfate, inhibitor NAM and fulvic acid potassium. The fertilizer has the advantages of balanced and comprehensive nutrition, smooth appearance, hardening prevention, long fertilizer efficiency, high utilization, labor output reduction, soil improvement and environmental pollution reduction.

The invention discloses a fruit swelling type seaweed organic liquid water soluble fertilizer, and belongs to the technical field of formula fertilizers of crops. The fruit swelling type seaweed organic liquid water soluble fertilizer is prepared from the following raw materials: a seaweed extracting solution, urea, ammonium polyphosphate, potassium nitrate, magnesium sulfate monohydrate, EDTA chelated iron, EDTA chelated manganese, EDTA chelated zinc, EDTA chelated copper, boric acid, ammonium heptamolybdate and potassium fulvic. The fruit swelling type seaweed organic liquid water soluble fertilizer is comprehensive in nutrition, scientific and reasonable in formula, fully water-soluble and easy to apply, improves soil, integrates fast acting and long acting, and effectively promotes the crop growth.

The invention relates to a preparation method of iron chelating peptide derived from hairtail fish protein. The preparation method concretely comprises the following steps: taking hairtail fish as a raw material, carrying out enzymolysis for the hairtail fish protein with papain and trypsin, carrying out ultrafiltration, macroporous resin column chromatography, gel column chromatography and reversed-phase high performance liquid chromatography separation and purification, and thus obtaining the iron chelating peptide Ala-Pro-Pro-Glu-Asn-Gly-Met-Ala-Gln-Met, wherein the ESI/MS determining molecular weight is 1045.18Da. Due to the unique chelating and transport mechanism, the iron chelating peptide of hairtail fish protein is high in absorption availability, can simultaneously supplement polypeptide/amino acid and iron, and is an ideal iron supplement substance.

A method is provided for the prevention and treatment of selective progressive degeneration within the central nervous system caused by hydroxyl-free or ferryl-free radicals formed by Fenton-type catalyzed reactions between diffusible hydrogen peroxide and localized bivalent iron. The invention embodies unique pharmacologic composition for antioxidant protection by oral supplementation with hypoxanthine conjointly with either sodium L-ascorbate or L-ascorbic acid. The hypoxanthine is provided for its sodium-dependent intestinal absorption and transport for the systemic production of higher antioxidant and iron-chelating uric acid levels. Ascorbate is provided as potent antioxidant to raise body ascorbic acid levels concurrently and to protect against possible deleterious effect from nucleobase or other molecular injury induced by oxidized uric acid as urate anion free radical caused in the antioxidant action of the uric acid. It is contemplated that such oral supplementation conjointly with hypoxanthine and L-ascorbate will support better health and will mitigate the progressive oxidative neuronal damage in Alzheimer's disease, amnestic mild cognitive impairment, Down syndrome, amyotrophic lateral sclerosis, and Parkinson's disease.

The invention discloses a red date iron replenishment drink containing peptide and iron-chelating peptide and a preparation method. The iron replenishment drink is prepared from, by weight, 1-2 parts of a mixed solution of a pig blood peptide and an iron-chelating peptide, 30-60 parts of red date pulp and 4-8 parts of a sweetening agent. The preparation method comprises the steps of 1, preparation of the mixed solution of the pig blood peptide and the iron-chelating peptide; 2, preparation of the red date pulp; 3, mixing and boiling. The red date iron replenishment drink has the advantages that absorption of iron contained in red dates can be facilitated due to the addition of the peptides, absorption of the iron-chelating peptide does not disturb the absorption of the iron, no simulation to a gastrointestinal tract is caused, the effects of preventing iron-deficiency anemia and other iron-deficiency diseases are achieved, and the red date iron replenishment drink is the good iron replenishment product.

The use of a novel mono-cationic salt of N,N'-bis(2-hydroxybenzyl)ethylenediamine-N,N'-diacetic acid (HBED) in iron chelating therapy is disclosed. In particular, the invention relates to the subcutaneous use of a mono cationic salt of HBED for treating a human with a disease treatable by an iron chelator such as iron overload, especially transfusional iron overload.

The invention relates to iron chelation application of Trichiutus haumela bone iron-chelated collagen peptide. Owing to a unique chelation and transport mechanism, the iron-chelated collagen peptide is high in absorption and utilization degree, also can supplement polypeptide/amino acid and iron and is an ideal iron supplement substance. According to the iron-chelated collagen peptide, Trichiutus haumela bone is taken as a raw material, collagen is extracted by an acid process and is enzymatically hydrolyzed by compound flavourzyme and neutral protease, and separation and purification are performed by ultrafiltration, immobilized iron ion affinity chromatography and reversed-phase high-performance liquid chromatography to obtain the iron-chelated collagen peptide Gly-Pro-Pro-Gly-Pro-Trp-Gly-Pro-His-Gly, and the molecular weight based on ESI/MS detection is 957.99Da.

The invention discloses a preparation methods euphausia superba iron chelate peptide. Euphausia superba powder is used as a raw material, degreasing, enzymolysis and membrane separation are performed,and a component with molecular weight smaller than 3000 Da is taken and subjected to vacuum freeze-drying to obtain the euphausia superba iron chelate peptide. The euphausia superba iron chelate peptide can bind with Fe<2+> to form euphausia superba iron chelate. The prepared euphausia superba iron chelate peptide has iron chelate rate up to 93%, can improve the bioavailability of iron in organisms, has good iron absorption promoting activity, can promote iron absorption and supplement organisms with essential amino acids for organisms compared with inorganic iron FeSO4, and also provides a new direction for high-value utilization of euphausia superba. The prepared euphausia superba iron chelate peptide can be applied to preparation of iron-rich food or iron supplements.

The present invention discloses an enteromorpha polysaccharide biological activity improving method comprising the following steps: enteromorpha polysaccharide is degraded by hydrogen peroxide-vitamin C combined method to obtain degraded enteromorpha polysaccharide (DEP); chloroacetic acid as a carboxymethylation reagent is reacted with the degraded enteromorpha polysaccharide (DEP) to prepare carboxymethylated enteromorpha polysaccharide (CDEP); and in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), the CDEP is coupled with hydroxylamine to obtain hydroxamic acid acidized degraded enteromorpha polysaccharide (HCDEP). The hydroxamic acid acidized degraded enteromorpha polysaccharide (HCDEP) has a relatively low molecular weight (30-13kDa), and iron-chelating capacity is 0.2-1.2mmol / g. The hydroxamic acid acidized degraded enteromorpha polysaccharide (HCDEP) has high in-vitro antioxidant activity and antibacterial activity.

A composition for reducing the inhibitory effects of contaminants on nucleic acid amplification is provided. The composition includes a plurality of zirconium oxide particles, a non-ionic surfactant,and an organic iron-chelating reagent. The organic iron-chelating reagent has a first affinity constant greater than or equal to 104.2 with respect to ferric iron and a second affinity constant less than 103.8 with respect to magnesium, wherein the first affinity constant and the second affinity constant are determined in deionized water at pH 8.45 and 20 DEG C. Optionally, the composition includes polyvinylpyrrolidone. Optionally, the composition comprises water. The composition has a pH of about 8.45 to 8.85. Methods of using the composition to prepare a sample for nucleic acid amplificationare also provided.

An environmentally responsible iron chelating additive and method for removing hydrogen sulfide or sulfide ions from drilling and packer fluids and preventing settling of precipitates. Iron chelating agents selected from the group consisting of ferrous lactate, ferrous gluconate, ferrous bis glycinate, ferrous citrate, ferrous acetate, ferrous fumarate, ferrous succinate, ferrous sacchrate, ferrous tartarate, ferrous glycine sulfate, ferrous glutamate, ferrous ascorbate, ferrous polymaltose, or a combination thereof, may be used. When the fluids are oil based, the iron chelating agents are added to the water phase of an emulsion, and the emulsion is added to the fluid. Viscosifiers may also be added to the drilling fluid with the emulsion.

The invention relates to a sturgeon bone gelatine iron chelating peptide and a preparation method thereof, and belongs to the technical field of deep processing of aquatic products. The iron chelatingpeptide is prepared by the following steps: using sturgeon bone as a raw material to prepare gelatin, utilizing trypsin and alkaline protease to perform enzymatic hydrolysis on the gelatin, separating and purifying to obtain the iron chelating peptide Asp-Gly-Pro-Lys-Gly-Pro-His-Gly-Lys-Asp-Gly-Arg(DGPKGPHGKDGR) by ultrafiltration, macroporous resin column chromatography, affinity chromatographyand reversed-phase high performance liquid chromatography, and detecting that the molecular weight is 1220.28 Da by ESI/MS. The iron chelating peptide provide by the invention has strong iron chelating ability and is used for preparing iron supplement medicines and health products.

The invention relates to a preparation method for rana temporaria skin small-molecule peptide chelated iron. The method uses a large amount of rana temporaria skin to conduct research on the rana temporaria skin chelated iron, not only properly handles the problem of the rana temporaria skin, but also can improve the utilization rate of the rana temporaria skin and improve economic and ecologicalbenefits. The method comprises the steps of crushing the rana temporaria skin, performing decolorization, performing degreasing, performing enzymolysis, performing ultrafiltration and performing chelation. The small-molecule peptide chelated iron is a novel generation of iron supplement products, a chitosan immobilization technology is used to stabilize enzyme activity, a double light wave-water bath composite enzyme assisted method is used to enzymatically hydrolyze the rana temporaria skin, the enzymatic hydrolysis time is short, and the efficiency is high; and the ultrafiltration method isused to prepare the small-molecule peptide solution, the small-molecule peptide solution and ferrous ions are subjected to chelation to obtain the small-molecule peptide chelated iron, the chelation can be performed without freeze-drying the peptide solution to form peptide powder, the method is simple, and the obtained small-molecule peptide chelated iron has a higher chelation rate, a higher absorption rate in human bodies, and a good iron supplementation effect; and the rana temporaria skin small-molecule peptide chelated iron prepared by the method is used in the fields of food, health care and biological pharmacy.

The invention relates to a method of preparing composite amino acid chelated iron-zinc from a sea cucumber intestine nanofiltration liquid. The method includes (1) a step of pretreating sea cucumber intestines, (2) a step of homogenizing the sea cucumber intestines, (3) a step of first enzymatic hydrolysis, (4) a step of secondary enzymatic hydrolysis, (5) a step of ultrafiltration, (6) a step of nanofiltration, (7) a step of iron chelating, (8) a step of zinc chelating, namely a step of measuring a proper amount of a concentrate liquid, adding a proper amount of a soluble zinc salt into the concentrate liquid under stirring at 35-45 DEG C, slowly adding a proper amount of an alkali to adjust the system pH to be 7.0-7.5 with reaction time being 2-3 h, maintaining the pH value to be consistent during the reaction, cooling after the reaction is finished, centrifuging, washing solids, drying and crushing to obtain composite amino acid chelated zinc, and (9) a step of mixing, namely a step of fully mixing sea cucumber intestine polypeptide chelated iron and sea cucumber intestine polypeptide chelated zinc according to a mass ratio of 1-2:1 to obtain the composite amino acid chelated iron-zinc. The prepared composite amino acid chelated iron-zinc is rich in nutrients, easy to absorb, easy to utilize, high in absorption and utilization rate and high in nutrition value.

The subject invention involves a method of extending the life span of mammals by reducing free radical damage in mammals wherein the method comprises administering to a mammal a composition comprising a safe and effective amount of a compound selected from 1-phenyl-1,2-propanedione-2-oxime, benzoylacetone, piroctone, 2-furildioxime, 2-furilmonoxime, diethyldithiocarbamic acid, deferoxamine and 1,2-dimethyl-3-hydroxy-pyrid-4-one, or a pharmaceutically acceptable salt thereof, or mixtures of the subject compounds and/or their salts.

The invention provides an ALA-HPO hybrid derivative shown as a formula (I) or a formula (II), and a preparation method thereof, and application of the ALA-HPO hybrid derivative in preparation of photodynamic therapy drugs. The formula (I) and the formula (II) are defined in the specification.

The invention relates to a chelating agent with high stability and high iron chelating ability and a preparation method thereof. The chelating agent is a target compound which is prepared from phenol derivatives, aliphatic diamine and glyoxalic acid aqueous solution serving as initial materials by mannich reaction in a one-step synthesis manner. By adopting the preparation method disclosed by the invention, the defects of fussy synthesis steps, low yield and poor selectivity of the traditional chelating agents such as EDDHA (ethylenediamine-N,N'-diacetic acid) are overcome; and the obtained chelate has higher stability and iron chelating ability relative to EDTA (ethylene diamine tetraacetic acid) and EDDHA.

A composition for reducing the inhibitory effects of contaminants on nucleic acid amplification is provided. The composition includes effective amounts of ferric iron, an organic iron-chelating reagent, and a non-ionic surfactant. Optionally, the composition includes polyvinylpyrrolidone. The composition has a pH of about 8.45 to 8.85. The organic iron-chelating reagent has a first affinity constant greater than or equal to 104.2 with respect to ferric iron and a second affinity constant less than 103.8 with respect to magnesium. The first affinity constant and the second affinity constant are determined in deionized water at pH 8.45 and 20DEG C. Methods of using the composition to prepare a sample for nucleic acid amplification are also provided.

The invention discloses trace element synergistic compound fertilizer. The compound fertilizer comprises the following components in parts by weight: 180 to 250 parts of urea, 100 to 150 parts of ammonium chloride, 300 to 330 parts of potassium chloride, 300 to 340 parts of monoammonium phosphate, 200 to 300 parts of calcium silicate, 80 to 100 parts of magnesium sulfate, 13 to 30 parts of trace element additives, 20 to 50 parts of organic matter additives, 15 to 30 parts of sludge and 10 to 15 parts of rare earth element, wherein the trace element additives comprise the following components in parts by weight: 3 to 5 parts of chelated zinc, 3 to 5 parts of chelated iron, 3 to 5 parts of chelated copper, 3 to 5 parts of boric acid, 0.5 to 2 parts of ammonium molybdate and 0.5 to 2 parts ofpolycarboxylic acid nickel. The trace element synergistic compound fertilizer provided by the invention is suitable for the middle and later period of rice production, can promote the growth of riceand can meet the requirements of the rice on different trace elements during growth.

The invention relates to an ultrasonic modified whey protein chelated ferrous iron supplementing agent and a preparation method thereof, and belongs to the technical field of biology. According to the invention, whey protein isolate and FeSO<4> are taken as raw materials; the whey protein isolate is modified through an ultrasonic technology; the iron chelating capacity of whey protein is taken as an index; ultrasonic modification conditions are optimized; finally, the in-vitro gastrointestinal stability of the whey protein is researched; and the iron supplementing agent which is easy to absorb and high in bioavailability is prepared. The whey protein isolate is subjected to ultrasonic treatment, so that the protein has good biological safety, biocompatibility and biodegradability and ideal functionality of chelating with iron; meanwhile, the problems that the solubility of the whey protein is reduced, the chelating capacity of the whey protein with iron is reduced and the like possibly caused by traditional heat treatment are avoided; the method has the advantages of high efficiency, environmental protection, good iron supplement effect, few side effects and the like. According to the invention, technical support and reference are provided for application of the whey protein chelated iron as the iron supplement.

The invention discloses a use of a 3-hydroxy-4-pyridone high-molecular iron chelating agent. The 3-hydroxy-4-pyridone high-molecular iron chelating agent has antibacterial effects and can be used as an antibacterial material. The 3-hydroxy-4-pyridone high-molecular iron chelating agent has excellent effects of resisting methicillin-resistant staphylococcus aureus. According to a mechanism of cation simulative antibacterial peptide formation from tertiary amine or secondary amine positive ion structures in a high-molecular iron chelating agent, interaction between a positive charge area and a negative charge area on a cell membrane, and iron chelating effects of an iron chelating agent, the normal growth environment around bacteria is destroyed and bacterium growth is inhibited so that bacteria cannot normally grow. The 3-hydroxy-4-pyridone high-molecular iron chelating agent cannot be absorbed by skin because of macro-molecule characteristics, does not produce toxic or side effects and can be used as an antibacterial material with dual antibiosis effects.

Modified hydrophilic carbon clusters (HCCs), poly(ethylene glycol)-hydrophilic carbon clusters (PEG-HCCs) and similarly structured materials like graphene quantum dots (GQDs), PEGylated GQDs, small molecule antioxidants, and PEGylated small molecule antioxidants. These materials have been modified with an iron chelating moiety, deferoxamine, or a similar chelating moiety. By exploiting common binding sites, the carbon nanostructure facilitates intracellular transport including in mitochondria, reduces oxidative breakdown of the chelator moiety prior to treatment, and reduces both the cause and consequences of metal induced oxidative stress within the body thus providing a novel form of therapy for a range of oxidative and metal-related toxicities. Graphenic materials can be used for the treatment of acute and chronic mitochondrial electron transport chain dysfunction.