35 results about "Iron Chelating" patented technology

The invention relates to a hemoglobin peptide capable of promoting iron absorption and a preparation method thereof, the mass content of polypeptide in the hemoglobin peptide is greater than 90%, and the hemoglobin peptide contains at least one of FERF and FDGL; the preparation method comprises the following steps: S1, adding trisodium citrate into fresh animal blood, centrifuging, adding a NaCl solution for washing, preparing the solution, and performing high-pressure homogenization wall breaking for 2-5 times to obtain a hemoglobin solution; s2, adding 10-200 U / mL of neutral protease, carrying out heat preservation treatment, then adding 5-50 U / mL of flavourzyme, carrying out enzymolysis, and carrying out enzyme deactivation to obtain an enzymatic hydrolysate; s3, filtering with an ultrafiltration membrane, and collecting permeate; s4, adjusting the pH value, adding into an anion exchange chromatographic column, eluting with a NaCl solution, and collecting the eluent; s5, desalting, concentrating and drying to obtain hemoglobin peptide; and S6, carrying out mass spectrometric detection, and screening bioactive peptides to obtain the oligopeptide with the activity of promoting iron absorption. The hemoglobin peptide obtained by the preparation method disclosed by the invention has relatively good iron chelation capability and iron absorption promoting capability, and the bioavailability of iron ions can be remarkably improved.

The invention relates to the technical field of small-molecule donkey-hide gelatin peptides, and provides a small-molecule donkey-hide gelatin peptide with iron ion chelating ability and application thereof, the molecular weight of the small-molecule donkey-hide gelatin peptide is smaller than 1000 Da, and the small-molecule donkey-hide gelatin peptide has the iron ion chelating ability. The iron chelation rate of the small-molecule donkey-hide gelatin peptide reaches 95%, and the small-molecule donkey-hide gelatin peptide has good solubility in in-vitro gastrointestinal digestion simulation and can be applied to the fields of iron supplements, nutrition fortified foods and the like.

The invention discloses a method for preparing nano calcium carbonate by efficiently removing iron impurities in nano calcium carbonate raw material ore, and belongs to the technical field of nano material preparation. The method comprises the steps of raw material pretreatment, digestion treatment, size mixing treatment, carbonization treatment, surface treatment and drying, in the size mixing treatment process, a whitening agent Na2SO3 is adopted for removing iron in raw material ore, Na2SO3 serves as a reducing agent, Fe < 3 + > is reduced into Fe < 2 + >, a proper amount of whitening auxiliary such as DTPA or triethanolamine forms colorless water-containing iron chelate ions with iron ions, and the iron chelate ions are subjected to surface treatment. And through a synergistic system of the whitening agent and the whitening auxiliary agent, efficient removal of iron is realized. The whiteness of the prepared nano calcium carbonate reaches up to 97.2%, the nano calcium carbonate has the remarkable advantages of being efficient, environmentally friendly, economical and the like, the iron content in the nano calcium carbonate can be remarkably reduced, the purity and whiteness of the product are effectively improved, and the high requirement of the high-end application field for the quality of the nano calcium carbonate is met. Meanwhile, the method is simple and convenient to operate and easy to industrially apply, and has wide market prospects and popularization value.

The present application relates to a milk-derived phosphorylated peptide with the activity of improving intestinal iron absorption and its application in the preparation of iron supplements. The sequence of the milk-derived phosphorylated peptide is EESITRINKKIE, and the glutamic acid at the amino terminal of the sequence is connected with 1-3 phosphoserines. The milk-derived phosphorylated peptide can be combined with Fe 2+ Specifically bound, strong iron chelating ability, and can significantly improve the iron transport amount in the Caco-2 small intestinal epithelial cell model, thereby having good activity of improving intestinal iron absorption, and can be widely applied in the fields of drugs, health care products or food.

This invention relates to the field of biomedical technology, providing a phosphorylated peptide iron chelate of Yanbian cattle hemoglobin, its preparation method, and its applications. The Yanbian cattle hemoglobin phosphorylated peptide iron chelate is prepared from Yanbian cattle blood through enzymatic hydrolysis, phosphorylation, and iron chelation. Specifically, the phosphorylation step actively introduces strongly negatively charged phosphate groups into the Yanbian cattle hemoglobin peptide chain, serving as new and stronger metal ion coordination sites, thereby specifically enhancing its chelating ability for ferrous ions and the stability of the complex. The Yanbian cattle hemoglobin phosphorylated peptide iron chelate possesses high iron content and good gastrointestinal stability, showing broad application prospects in the preparation of a new generation of highly efficient and low-irritant iron supplements.

The preparation process comprises the following steps: S100, mixing humic acid, monoammonium phosphate and potassium sulfate to obtain a first mixture; s200, mixing the first mixture with the blue-green algae extract to obtain a second mixture; s300, mixing the second mixture, sodium alginate, chelated zinc, chelated iron and chelated magnesium to obtain a third mixture; s400, mixing peroxidized humic acid, magnesium sulfate and monopotassium phosphate to obtain a fourth mixture; and S500, adding the fourth mixture into the third mixture to obtain the blue-green algae organic fertilizer. The cyanobacteria water-soluble fertilizer prepared by the process is good in absorption, obvious in fertilization effect and small in fertilization amount, and manpower and time used for fertilization can be reduced.

Provided is a water-absorbing resin composition having both gel stability of the water-absorbing resin composition when a hygienic material is usually worn and high handleability when the water-absorbing resin composition is subjected to a separation treatment after being irradiated with ultraviolet rays as a waste treatment means. The water-absorbing resin composition including water-absorbent resin particles and a chelating agent. The water-absorbent resin particles are surface-crosslinked, and the chelating agent has an iron (III) chelate stability constant of 16 log KML or more and 24 log KML or less, and 4 or more and 10 or less carboxyl groups.

The present invention provides a liposome encapsulating L-arginine / Prussian blue nanoparticles and its preparation method and application, which belongs to the field of biological preparation technology. The present invention deposits L-arginine on hPB through iron chelation, and then coats liposomes to avoid leakage of water-soluble L-arginine in the circulation, thereby obtaining liposomes encapsulating L-arginine / Prussian blue nanoparticles. Due to the EPR effect, hPFL@Lipo in the present invention can be efficiently delivered and distributed to tumor tissue, and a large number of tumor cells are ablated by heat under near-infrared light irradiation conditions, which greatly limits the utilization of L-arginine by tumor cells, while inducing immunogenic death, releasing DAMPS in situ, further promoting anti-tumor T cell infiltration and activation, and enhancing tumor immunotherapy. And under light irradiation, the liposome disintegrates and releases L-arginine, providing nutritional support for immune-active cells at a competitive disadvantage, and promoting the activation of the immune microenvironment.

The invention discloses a preparation method of iron-chelated ovotransferrin and an iron supplementing product thereof. The preparation method comprises the following steps: (1) pre-treating egg white: treating egg white liquid through an ultrasonic composite high-voltage pulse electric field; (2) removal of ovomucin and ovalbumin: removing the ovomucin and ovalbumin by an isoelectric precipitation method combined with a PEG precipitation method; (3) preparation of ovotransferrin; (4) separating an ovotransferrin crude product; (5) purifying an ovotransferrin crude product; (6) pre-treating the iron-chelated ovotransferrin; and (7) preparing the iron-chelated ovotransferrin: adopting an ultrasonic composite high-voltage pulsed electric field twice. The method disclosed by the invention is quick, efficient, high in ovotransferrin iron binding capacity, simple and low in cost, can be used for preparing iron-supplementing medicines and health-care foods, is beneficial to supplementing iron elements and enhancing the immunity of the organism, and is suitable for large-scale industrialization.

One embodiment provides a method for measuring zinc in an aqueous sample, the method comprising: adding an iron chelating agent to the aqueous sample; chelating an amount of iron in the aqueous sample with the iron chelating agent; and measuring the amount of zinc in the aqueous sample by measuring a change in absorbance intensity of the iron-chelated aqueous sample. Other aspects are described and claimed.

The invention relates to the technical field of functional food, in particular to a polypeptide composition for regulating menstruation and nourishing blood and a preparation method thereof, and the polypeptide composition comprises the following raw materials: a Chinese wolfberry extract, an astaxanthin-fish oil co-coated preparation, a red algae oligosaccharide iron complex, maltodextrin and folic acid. In the polypeptide composition for regulating menstruation and nourishing blood and the preparation method thereof, the astaxanthin-fish oil co-coated preparation stably wraps the strong antioxidant astaxanthin and the fish oil through a complex coacervation microencapsulation technology, so that excessive free radicals generated by menstrual physiological stress can be efficiently eliminated, inflammatory response of uterine tissues can be relieved, and discomfort symptoms such as dysmenorrhea and the like can be relieved; according to the red algae oligosaccharide iron complex, oligosaccharide is used as a natural carrier of an iron element and is absorbed in an oligosaccharide iron chelating form in intestinal tracts, the absorption way of traditional ferric salt is avoided, the bioavailability is greatly improved, the iron element can be efficiently and mildly supplemented, and iron-deficiency anemia is improved.

An alcohol-free method of extracting β-glucans such as lentinan from mushrooms is described. The method excludes the drying and pulverising of the mushrooms prior to extraction. The simultaneous use of enzymatic digestion and ultrasonication provides extracts with high levels of desired properties including 1,1-diphenyl-2-picryl hydrazyl radial scavenging activity, superoxide radical scavenging activity, iron chelating capacity, and ferric-reducing antioxidant power.

This invention belongs to the field of biomedical technology, specifically relating to a method for preparing bioactive polypeptides and their applications. This invention prepares bioactive polypeptides from donkey-hide gelatin protein through an enzymatic hydrolysis reaction. The bioactive polypeptides possess iron-chelating ability, enhancing Hb and RBC production in a mouse anemia model. Therefore, the polypeptides of this invention have blood-nourishing and blood-tonifying functions, are non-toxic, have no side effects, and also have advantages such as simple preparation, easy absorption, and ease of widespread application.

The invention belongs to the technical field of mineral processing and high-purity material preparation, and particularly relates to a grading purification system for low-impurity quartz sand and a production method of the grading purification system. The method comprises the following steps: preparing the following components in parts by mass: 800-1200 parts of natural quartz sand, 10-15 parts of silanized magnetic nano ferrum dioxide, 5-8 parts of 3-hydroxypyridine, 0.5-1.5 parts of a polyacrylamide flocculating agent, 0.4-0.8 part of a lauryl sodium sulfate dispersing agent, 2-4 parts of an activated carbon decolorizing agent, 8-12 parts of a sodium silicate builder and 0.3-0.7 part of a sodium hydroxide solution. The technological process sequentially comprises the steps of quartz sand grading, washing and size mixing, alkaline pH regulation and control, air flotation impurity removal, magnetic separation iron removal, chelating sedimentation and vacuum drying. The modified magnetic nano material and the high-selectivity chelating agent are introduced to synergistically remove Fe2O3 and magnetic impurities, so that the purification efficiency is effectively improved. The purity of the finally obtained quartz sand can reach 99.88%, and the quartz sand is suitable for preparing high-purity raw materials in the industries of electronics, photovoltaics and semiconductors.

The invention comprises a process and composition relating to the use of an artificial blood that is a pharmaceutically acceptable solution of an iron amino acid chelate or a polymerized amino acid chelate. The composition is directed to an artificial blood composition that is free or substantially free of hemoglobin made from one or more amino acids known to be bound to the hemoglobin molecule but free of hemoglobin for purposes of this invention. The amino acids are chelated with iron (ferrous or ferric), oxygenated, and used as an artificial blood replacement for whole blood or as a component of an artificial blood composition. The iron component of the amino acid chelate is oxygenated to enable the chelate to carry oxygen throughout the body. To form a solution suitable for transfusion, the oxygen-bearing iron chelate of the amino acid is dissolved, to the extent necessary, preferably in an isotonic saline solution. In use, the artificial blood composition is transfused into an individual suffering trauma due to loss of blood using conventional transfusion procedures known in the art.

The invention provides a special multi-growth-period all-element AB water-soluble fertilizer for planting chives in a field and a use method of the water-soluble fertilizer. The water-soluble fertilizer is prepared from the following raw materials in parts by weight: calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, monopotassium phosphate, magnesium sulfate heptahydrate, ammonium sulfate, chelated iron, chelated manganese, chelated zinc, chelated boron, chelated copper and sodium molybdate. Water-soluble fertilizer and irrigation water are supplied synchronously, and water and fertilizer are integrated. According to the method, the quality of chive products is improved, and meanwhile, the investment cost is reduced.

The invention relates to the technical field of biology, and discloses a composite cordyceps protein peptide, a preparation method and application thereof, and a method for preparing an iron chelating peptide. The composite cordyceps sinensis protein peptide comprises one or more of cordyceps sinensis protein peptides with the amino acid sequences as shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20 and SEQ ID NO.21. The invention further discloses a preparation method of the composite cordyceps sinensis protein peptide. The peptide can be efficiently chelated with iron ions, the composite cordyceps protein peptide is obtained from cordyceps mycelia through a microbial fermentation method and is chelated with iron salt, and the obtained iron chelating peptide has the capacity of promoting the absorption of the intestinal tract to the iron ions, so that the safe and efficient treatment effect is achieved, and the obvious prospect is achieved.

A composition for efficient metal chelation includes (i) a primary chelant; (ii) a coordination enhancing additive; and (iii) a solvent. A wellbore servicing fluid includes (i) a primary chelant; (ii) a coordination enhancing additive; and (iii) a solvent.

The invention discloses application of deferasirox in preparation of a medicine for preventing ventricular remodeling after acute myocardial infarction, and relates to the technical field of medicines, in particular to application of deferasirox in preparation of a medicine for preventing ventricular remodeling after acute myocardial infarction. The medicine plays a role in inhibiting ferroptosis and inflammatory reaction of myocardial cells so as to prevent ventricular remodeling after acute myocardial infarction; deerasirox plays an iron chelation role and an anti-oxidative stress injury role in the medicine so as to inhibit ferroptosis and inflammatory response of myocardial cells; the inhibition effect of the medicine on myocardial cell ferroptosis is that GPX4 protein is up-regulated; the dosage of deferasirox in the medicine is 30 mg / Kg every day; the deferasirox can prevent ventricular remodeling after myocardial infarction of mice by inhibiting ferroptosis and inflammatory response, including improvement of cardiac function indexes and reduction of myocardial infarction area, and a new idea and a new scheme are provided for clinical prevention of ventricular remodeling after acute myocardial infarction.

A hemoglobin peptide and preparation method thereof that can promote iron absorption, the mass content of polypeptide in the hemoglobin peptide is greater than 90%, containing at least one of FERF and FDGL;Preparation method includes: S1, after adding trisodium citrate to fresh animal blood, centrifugation adds NaCl solution to wash, after preparing solution, high pressure homogenization broken wall 2 5 times, obtain hemoglobin solution;S2, after adding 10 200 U / mL neutral protease heat treatment, then add 5 50 U / mL flavor protease enzymolysis, deactivate enzyme to obtain enzymolysis solution;S3, ultrafiltration membrane filtering, collect permeate;S4, after adjusting pH, add in anion exchange chromatography column, NaCl solution elution, collect eluate;S5, desalination, concentration, obtain hemoglobin peptide after drying;S6, mass spectrometry detection, bioactive peptide screening, obtain short peptide with promoting iron absorption activity. The hemoglobin peptide obtained by the preparation method of the present invention has good iron chelating ability and promotes iron absorption ability, and can significantly improve the bioavailability of iron ions.

The invention discloses an extraction method and application of ferrous chelated heptapeptide, and belongs to the technical field of biology. The heptapeptide disclosed by the invention is clear and definite in structure, can be prepared by adopting a solid-phase chemical synthesis method, and also can be obtained by separating and purifying fermented scallop skirt hydrolysate. The heptapeptide provided by the invention has a remarkable improvement effect on symptoms caused by iron-deficiency anemia. Experimental results show that the heptapeptide can promote hemoglobin synthesis and regulate expression of related genes of anemia individuals. The method is suitable for developing novel iron-rich foods and iron supplements, and can also be compounded with other health-care products or food additives for use.

The invention discloses a walnut polypeptide, the amino acid sequence of the walnut polypeptide is Leu-Leu-Glu-Cys-His-Ser, the walnut polypeptide LLECHS has high iron chelating ability, ultrasonic-assisted chelating can enhance the iron chelating ability of the LLECHS, and the walnut polypeptide LLECHS can be used for preparing an iron chelating agent, does not generate toxic or side effect on the body, and is safe and edible.

The invention relates to the technical field of peptide iron chelation processing, in particular to a preparation method of a phycocyanin polypeptide-ferrous chelate, which comprises the following steps: taking phycocyanin freeze-dried powder as a raw material, and carrying out enzymolysis by adopting protease combined with ultrasound to obtain phycocyanin polypeptide; dissolving with pure water to obtain phycocyanin polypeptide, adding ascorbic acid, and heating in a water bath; centrifuging a chelation reaction solution, taking supernate, adding ethanol, standing, centrifuging again, collecting precipitate, and performing vacuum freeze drying to obtain phycocyanin polypeptide-ferrous chelate powder; the phycocyanin polypeptide-ferrous chelate prepared by the invention has higher iron content, shows more excellent stability in the process of simulating gastrointestinal digestion in vitro compared with inorganic iron salt, further has triple functions of supplementing iron, resisting oxidation and supplementing essential amino acids, is prepared from food-grade raw materials, is safe in process and low in cost, and is suitable for industrial production. A low-cost and high-efficiency scheme is provided for IDA intervention, and the method can be widely applied to development of foods, medicines and health-care products.

The invention discloses extraction and application of an octapeptide capable of resisting gastrointestinal digestion, and belongs to the technical field of biology. The octapeptide disclosed by the invention is clear and definite in structure, can be prepared by adopting a solid-phase chemical synthesis method, can also be synthesized by recombinant expression of genetically engineered bacteria, and can also be obtained by separation and purification from fermented scallop skirt hydrolysate. After the octapeptide-ferrous chelate is digested by artificial saliva, artificial gastric juice and artificial intestinal juice, the retention rate of the octapeptide-ferrous chelate is high. The chelate of octapeptide and ferrous iron can improve the serum iron content of mice with iron-deficiency anemia and reduce the total iron binding force of serum. And compared with ferrous sulfate, the iron-rich protein has higher hemoglobin regeneration efficiency and relative biological value, is further suitable for developing novel iron-rich foods and iron supplements, and can also be compounded with other health-care products or food additives for use.

The invention discloses application of a g-C3N4 nanosheet in preparation of a medicine for preventing and treating male reproductive dysfunction, and belongs to the technical field of biological medicine. The invention provides a multi-site iron chelation strategy, namely, the g-C3N4 nanosheet is used for inhibiting iron death induced by iron overload and preventing male reproductive dysfunction. An in-vitro result shows that the g-C3N4 nanosheet has an iron ion response fluorescence quenching effect and shows remarkable recognition specificity and sensitivity; in-vivo studies show that iron overload can cause germ cell injury and cause sterility, however, the g-C3N4 nanosheet relieves iron accumulation by activating an Nrf2 / GPX4 pathway, inhibits iron apoptosis and recovers fertility.

Provided are: a water-absorbent resin composition which has excellent decomposition performance while having high water-stop performance, and which can be suitably used as a water-stop material; and a water-stop material which contains the water-absorbent resin composition. This water-absorbent resin composition contains water-absorbent resin particles and a chelating agent, and the chelating agent has an iron (III) chelating stability constant (logKML) of from 19.0 to 24.0 (inclusive) and a 30-second swelling height of 6.0 mm or more. The water-absorbent resin composition of the present invention has excellent decomposition performance while having high waterstop performance, and can be suitably used as a waterstop material.

The invention relates to a method for increasing the ferrous chelating amount of ovotransferrin by using low-temperature plasma, which comprises the following steps of: preparing ovotransferrin by using fresh egg white as a raw material and adopting cation exchange chromatography; preparing a protein solution with a certain concentration, sequentially adding NaHCO3 and FeSO4 solutions, and adjusting the pH value of the mixed solution; under a certain temperature condition, low-temperature plasma with certain power is adopted for treatment, and the ovotransferrin ferrous chelate with the high chelation rate is prepared within a certain reaction time range. The low-temperature plasma treatment improves the ferrous chelating amount of ovotransferrin, not only conforms to the development trend of modern nutritional supplements, but also provides a new technical path for solving the global health problem of iron-deficiency anemia.

Modified hydrophilic carbon clusters (HCCs), poly(ethylene glycol)-hydrophilic carbon clusters (PEG-HCCs) and similarly structured materials like graphene quantum dots (GQDs), PEGylated GQDs, small molecule antioxidants, and PEGylated small molecule antioxidants. These materials have been modified with an iron chelating moiety, deferoxamine, or a similar chelating moiety. By exploiting common binding sites, the carbon nanostructure facilitates intracellular transport including in mitochondria, reduces oxidative breakdown of the chelator moiety prior to treatment, and reduces both the cause and consequences of metal induced oxidative stress within the body thus providing a novel form of therapy for a range of oxidative and metal-related toxicities. Graphenic materials can be used for the treatment of acute and chronic mitochondrial electron transport chain dysfunction.

Disclosed herein is an iron chelating polymer for the treatment of a disease involving an iron-mediated pathology, the polymer comprising the reaction product of a first monomeric unit and a second monomeric unit polymerized by a reversible addition-fragmentation chain transfer mechanism using a suitable addition-fragmentation chain transfer agent, the first monomeric unit being selected from the group consisting of compound (I): [Formula 1] Represented by TIFF2025510226000031.tif42128, The second monomer unit is independently selected from the group consisting of 1-vinyl-2-pyrrolidone, acrylic acid, methyl methacrylate, N,N-dimethyl-acrylamide, ethyl methacrylate, N-vinylimidazole and styrene, the iron chelating polymer is dissolved in an aqueous medium, the iron chelating polymer has a molecular weight of at least about 1500 Da prior to chelation, and the iron chelating polymer includes one or more intramolecular hexadentate ligands for chelating iron. The described polymers are useful for the treatment of diseases associated with excess reactive iron.

This invention belongs to the field of bioactive peptide preparation, specifically relating to an iron chelating peptide, its preparation method, and its application in the preparation of iron supplements. This study used soybean protein as raw material and screened four iron chelating peptides—EDEPFNLRSR, EDEPFNLR, DDLELKKIR, and EEQPLEVR—through enzymatic hydrolysis, purification, identification, and molecular docking techniques. Using a Caco-2 small intestinal epithelial cell model, it was found that all of the above iron chelating peptides significantly promoted iron transport and absorption, and significantly promoted Fe2+ absorption. 2+ Retention in the Caco-2 cell monolayer. The novel iron chelating peptides screened from soybean protein hydrolysates in this invention hold promise as a safe and effective iron supplement.