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Preparation method for micromolecular astragalus phytochelatin

A technology of chelating peptides and small molecules, applied in the field of biological peptide separation, can solve the problems of astragalus astragalus not fully exerting its efficacy, incomplete extraction of heat-sensitive components, easy damage, etc., so as to improve the chelation efficiency, make up for the low content of peptides, and improve stability. sexual effect

Active Publication Date: 2019-03-12
河北肽都生物科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem that in the existing astragalus extraction process, the active ingredients are not fully extracted and the heat-sensitive components are easily destroyed, resulting in the inability to fully exert the efficacy of astragalus, the present invention provides a method for preparing a small-molecule astragalus chelating peptide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A preparation method of small molecule astragalus chelating peptide:

[0040] Step 1. Select 100 g of dried Astragalus membranaceus, crush it, add 700 ml of purified water, stir evenly, and decoct at 90°C for 5 hours to obtain a decoction mixture;

[0041] Step 2: Cool the decoction mixture to 55°C, add 1g of pectinase and 0.5g of α-L-rhamnosidase, and stir at a speed of 75r / min for 20 minutes to obtain the first enzymolysis solution ;

[0042] Step 3: Add 1 g of Luo Han Guo protease, 0.5 g of phytase and 0.4 g of laccase to the first enzymolysis solution, stir evenly, heat up to 55° C., and stir the enzymolysis at a speed of 75 r / min for 4 hours to obtain the second enzymatic solution;

[0043] Step 4: Warm up the second enzymolysis solution to 90°C, and inactivate the enzyme for 15 minutes; after inactivating the enzyme, use a decanter centrifuge to filter and remove the enzymolysis residue, and the rotational speed of the decanter centrifuge is 3600r / min; Centrifu...

Embodiment 2

[0048] A preparation method of small molecule astragalus chelating peptide:

[0049] Step 1. Select 10Kg of dried Astragalus membranaceus, crush it, add 75L of purified water, stir evenly, and decoct at 92°C for 4.5 hours to obtain a decoction mixture;

[0050] Step 2, cooling the decoction mixture to 56°C, adding 120g of pectinase and 60g of α-L-rhamnosidase, and stirring at a speed of 75r / min for 25min to obtain the first enzymolysis solution;

[0051] Step 3: Add 120g Luo Han Guo protease, 60g phytase and 50g laccase to the first enzymolysis solution, stir evenly, raise the temperature to 56°C, and stir the enzymolysis at a speed of 75r / min for 3.5h to obtain the second enzyme Solution;

[0052] Step 4: Warm up the second enzymolysis solution to 92°C, and inactivate the enzyme for 12 minutes; after inactivating the enzyme, use a decanter centrifuge to filter and remove the enzymolysis residue, and the rotational speed of the decanter centrifuge is 3600r / min; Centrifuge fi...

Embodiment 3

[0057] A preparation method of small molecule astragalus chelating peptide:

[0058] Step 1. Select 100Kg of dried Astragalus membranaceus, crush it, add 800L of purified water, stir evenly, and decoct at 95°C for 4 hours to obtain a decoction mixture;

[0059] Step 2: Cool down the decoction mixture to 58°C, add 1.5Kg of pectinase and 800g of α-L-rhamnosidase, and stir the enzymolysis at 75r / min for 20min to obtain the first enzymolysis solution ;

[0060] Step 3: Add 1.5Kg Luo Han Guo protease, 800g phytase and 600g laccase to the first enzymolysis solution, stir evenly, heat up to 58°C, and stir the enzymolysis at a speed of 75r / min for 3 hours to obtain the second enzyme Solution;

[0061] Step 4: Warm up the second enzymolysis solution to 95°C, and inactivate the enzyme for 10 minutes; after inactivating the enzyme, use a decanter centrifuge to filter and remove the enzymolysis residue, and the rotational speed of the decanter centrifuge is 3600r / min; Centrifuge filtra...

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Abstract

The invention provides a preparation method for micromolecular astragalus phytochelatin. The preparation method comprises the following steps of crushing astragalus and performing decoction with water; then adding pectinase and alpha-L-rhamnosidase, performing stirring for enzymolysis; then adding grosvenor momordica protease, phytase and laccase, and performing continuous enzymolysis; and performing enzyme deactivation on an enzymatic hydrolysate, performing separation and purification, adding soybean peptide, rising the temperature to 45-50 DEG C, performing stirring for 30-45 min to obtainchelating liquid, and concentrating and drying the chelating liquid to obtain the micromolecular astragalus phytochelatin. For the astragalus phytochelatin product prepared with the preparation method, the purity is high, the content of peptide with the peptide molecular weight of 180-500 daltons can reach 99 percent or above; through enzymolysis of compound enzyme, an allergen in astragalus and substances which are difficultly digested and utilized by a human body in the astragalus are degraded, so that the problem that stomach burden is aggravated by taking theastragalus for a long time is effectively avoided, and the micromolecular astragalus phytochelatin can be widely applied to the fields of foods, health care products, drugs and the like.

Description

technical field [0001] The invention relates to the technical field of separation of biological peptides, in particular to a preparation method of a small molecule astragalus chelating peptide. Background technique [0002] A variety of amino acids and minerals are essential nutrients for the human body, and these nutrients are mainly obtained from food. After food enters the human body and is digested by the stomach and small intestine, the protein is hydrolyzed into amino acids and some small molecule short peptides. The absorption of small peptides and free amino acids are two independent transport systems. It has the characteristics of low energy, not easy to saturate, and the transport between various peptides is non-competitive and inhibitory. The latest research shows that small peptides can improve the absorption and utilization of mineral elements, that is, small peptides can form chelates with Ca, Zn, Cu, Fe and other mineral elements to increase their solubility ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 张晓东杨广杨子丰
Owner 河北肽都生物科技集团有限公司
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