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Calcium phytochelatin and preparation method thereof

A technology of calcium chelation and calcium chelation, which is applied in the field of calcium chelating peptides, and can solve the problems that the calcium deficiency situation has not been fundamentally improved, reduced calcium bioavailability, and low calcium bioavailability

Inactive Publication Date: 2017-06-20
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, with the rapid development of calcium supplement reagents, sales have increased year by year, but people's calcium deficiency has not been fundamentally improved, the main reason is that the bioavailability of calcium is low
As long as the dietary composition of our country is plant-based food, oxalic acid, phosphoric acid and phytic acid in plants are easy to combine with calcium ions in the intestine to form insoluble oxalate, phosphate and phytate, thereby reducing the calcium content in the body. bioavailability
In addition, some inorganic calcium needs to be decomposed by gastric acid, which makes the bioavailability of calcium even lower for some people with abnormal gastric acid secretion

Method used

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  • Calcium phytochelatin and preparation method thereof
  • Calcium phytochelatin and preparation method thereof

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Embodiment 1

[0014] The instruments and detection methods used are as follows:

[0015] The Schizochytrium meal used in this technique was provided by Fujian Fisheries Research Institute, and the enzyme was purchased from Novozymes Biotechnology Co., Ltd. (Tianjin, China). Firstly, the protein of the schizochytrium meal was extracted, and the schizochytrid meal was processed with a traditional Chinese medicine pulverizer, and passed through a 60-mesh sieve to obtain raw materials for the experiment. The protein in Schizochytrium meal was extracted by alkali extraction and acid precipitation. Prepare a NaOH solution with a concentration of 0.39mol / L, and extract at 90°C for 30min according to a solid-liquid ratio of 1:100 (w / v). The obtained sample solution was centrifuged at 10,000 r / min for 20 min at 4°C, and the supernatant was taken to discard the precipitate. Then use 6mol / L HCl to adjust the pH value in the supernatant to 3.0, and carry out acid precipitation on the protein. After ...

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Abstract

The invention provides a calcium phytochelatin and a preparation method thereof. Schizochytrium limacinum meal protein is used as raw materials; alkaline protease and flavourzyme are used for stepped enzymolysis; then, separation and purification are performed to obtain the purified specific calcium-calcium phytochelatin; the amino acid whole sequence is yl. The prepared phytochelatin can be used for producing a calcium nutrition supplementing agent; the unique chelation mechanism and operation mechanism are realized; the absorption is easy; safety, nontoxicity and no side effect are realized; the price is low; the amino acid and the calcium ions can be supplemented at the same time; the calcium phytochelatin can certainly become the first choice of the calcium supplementing agent. The invention provides a fire-new idea for the application of the schizochytrium limacinum meal protein.

Description

technical field [0001] The invention relates to a calcium chelating peptide, more specifically to a calcium chelating peptide prepared by enzymatically degrading Schizochytrium meal protein step by step with alkaline protease and compound flavor protease, and belongs to the field of biotechnology. Background technique [0002] Microalgae has been identified by the Food and Agriculture Organization of the United Nations as a green and healthy food in the 21st century. Microalgae is not only rich in nutrients, but also has excellent health care effects. Schizochytrium belongs to microalgae-derived organisms, which are rich in proteins. These proteins are rich in glutamic acid and aspartic acid that can provide coordination bonds, and can effectively act as calcium ion ligands to form chelates with calcium elements. Compound, while supplementing trace elements, can make full use of the high nutritional value of Schizochytrium protein. [0003] Currently, calcium deficiency is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/065C12P21/06C07K1/20C07K1/16
CPCC07K5/06078C12P21/06
Inventor 汪少芸蔡茜茜徐梁棕陈旭方卫东
Owner FUZHOU UNIV
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