A kind of preparation method of small molecule Folium Folium chelating peptide

A kind of technology of Daqingye, chelated peptide

Active Publication Date: 2020-09-18
河北肽都生物科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of low utilization rate of active ingredients, slow onset of effect, and ingredients that are not easily digested and absorbed or easily sensitized in the existing Folium Folium extraction method, the present invention provides a method for preparing a small molecule Folium Folium chelating peptide

Method used

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  • A kind of preparation method of small molecule Folium Folium chelating peptide
  • A kind of preparation method of small molecule Folium Folium chelating peptide
  • A kind of preparation method of small molecule Folium Folium chelating peptide

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Embodiment 1

[0024] This embodiment provides a method for preparing a small molecule Folium chelatin, which specifically includes the following steps:

[0025] Step a, select 100g of Folium folium, soak in 700ml of purified water, and steam at 90°C for 4 hours;

[0026] Step b. Cool down to 55°C, add 1g of Bacillus subtilisin, stir at 55°C for 3 hours for enzymolysis; after the enzymolysis is complete, raise the temperature to 90°C, and keep for 10 minutes to inactivate the enzyme;

[0027] Step c, centrifuge at the rotating speed of 3400r / min with decanter centrifuge, filter and remove solid matter; Then use three-phase centrifuge to centrifugally purify at the rotating speed of 18000r / min; Centrifugate is filtered with nanofiltration machine (nanofiltration membrane aperture is 1.0nm);

[0028] Step d, stirring and adding 10 g of soybean oligopeptide and 5 g of sponge polypeptide into the nanofiltrate, then raising the temperature to 45° C., and stirring at a speed of 36 r / min for 30 mi...

Embodiment 2

[0031] This embodiment provides a method for preparing a small molecule Folium chelatin, which specifically includes the following steps:

[0032] Step a, select 10kg of Folium foliage, immerse in 75L of purified water, and steam at 92°C for 4.5h;

[0033] Step b. Cool down to 56°C, add 120g of Bacillus subtilisin, stir at 55°C for 3.5 hours for enzymolysis; after the enzymolysis is completed, raise the temperature to 92°C, and keep for 12 minutes to inactivate the enzyme;

[0034] Step c, centrifuge at the rotating speed of 3600r / min with decanter centrifuge, filter and remove solid matter; Then use three-phase centrifuge to centrifugally purify at the rotating speed of 18000r / min; Centrifugate is filtered with nanofiltration machine (nanofiltration membrane aperture is 0.8nm);

[0035] Step d, stirring and adding 1 kg of soybean oligopeptide and 0.6 kg of sponge polypeptide into the nanofiltration liquid, then raising the temperature to 46 ° C, and stirring at a speed of 35...

Embodiment 3

[0038] This embodiment provides a method for preparing a small molecule Folium chelatin, which specifically includes the following steps:

[0039] Step a, select 100kg of Daqing leaves, immerse in 800L of purified water, and steam at 95°C for 5h;

[0040] Step b. Cool down to 58°C, add 1.5kg of Bacillus subtilisin, and stir at 55°C for 4 hours; after the enzymolysis is completed, heat up to 95°C and keep for 15 minutes to kill the enzyme;

[0041] Step c, centrifuge at the rotating speed of 3800r / min with decanter centrifuge, filter and remove solid matter; Centrifuge and purify at the rotating speed of 18000r / min with three-phase centrifuge again; Centrifugate is filtered with nanofiltration machine (nanofiltration membrane aperture is 1.2nm);

[0042] Step d, stir and add 13.3kg soybean oligopeptide and 6.7kg sponge polypeptide into the nanofiltrate, then heat up to 50°C, and stir at a speed of 45r / min for 45min;

[0043] Step e, concentrating the chelating solution obtain...

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Abstract

The invention relates to the technical field of folium isatidis deep processing, in particular to a preparation method of small molecule folium isatidis phytochelatin. According to the method, foliumisatidis is taken as the primary raw material, and a small molecule phytochelatin product is obtained after steaming processing, neutral protease enzymolysis, solid liquid separation, purification, nanofiltration, polypeptide chelation and drying are conducted. The method has the advantages that the production cycle is short, the cost is low, the method has no pollution on the environment, the safety performance of a product is high, the purity is high, the molecular weight of the product is less, utilization rate of the effective ingredient is high, the effect comes up fast, and the small molecule folium isatidis phytochelatin can be applied to the fields of biological feed additives, drugs and the like.

Description

technical field [0001] The invention relates to the technical field of deep processing of Folium folium, in particular to a preparation method of small molecule Folium folium chelating peptide. Background technique [0002] Folium Isatidis contains flavonoid glycosides-mountain, geranthin, indigo magnolia, indigo, isatin B, indirubin, xylofuranose sweet acid and other components, as well as inorganic elements such as zinc, iron, calcium, magnesium, etc. It has the functions of clearing away heat and detoxification, cooling blood, eliminating plaque, relieving throat, and broad-spectrum antibacterial. It is a traditional Chinese herbal medicine for reducing fever, antibacterial, anti-inflammatory, and analgesic. It is mainly used for fever, headache, sore throat, rash, and carbuncle in febrile diseases Diseases such as sores. In addition, Folium Folium also contains a variety of antiviral substances, which have strong inhibitory and killing effects on cold viruses, mumps vir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06
Inventor 卢光明张晓东杨广杨子丰
Owner 河北肽都生物科技集团有限公司
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