Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of horse-faced puffer fish skin zinc chelating peptide

A horse-faced fish and chelating peptide technology, which is applied in the field of peptides of aquatic product processing products, can solve blank problems and other problems

Active Publication Date: 2020-10-23
北京姿美堂生物技术股份有限公司
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the applicant found that the preparation of horse-faced fish skin zinc chelating peptide and its technology research using horse-faced fish skin as a raw material is in a blank

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of horse-faced puffer fish skin zinc chelating peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] 1) Preparation of horse-faced fish skin gelatin: green-finned horse-faced fish ( Navodon septentrionalis ) minced fish skin, added to 0.3% NaOH solution according to the ratio of solid to liquid 1 g:15 mL, stirred for 5 h, washed with double distilled water until neutral; then added to 0.4% H2O according to 1 g:10 mL 2 SO 4 soaked at room temperature for 2 days, and changed the water every 8 hours; finally, the treated fish skin homogenate was added to double distilled water according to the ratio of solid to liquid at 1:10, extracted dynamically at 70°C for 15 hours, and centrifuged at 6 000 g for 30 min, take the supernatant, and freeze-dry to obtain horse-faced puffer fish skin gelatin.

[0020] ) Enzymolysis of horse-faced puffer fish skin gelatin: Add horse-faced fish skin gelatin into barbiturate sodium-hydrochloric acid buffer according to the ratio of solid to liquid 1 g: 12 mL, adjust the pH value to 7.0, and store at 50 °C Incubate for 10 min, add neutral pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for preparing thamnaconus modestus skin zinc phytochelatin. The method specifically comprises the following steps: by taking thamnaconus modestus skin as a raw material, removing non-collagen and minerals in the fish skin by using acid and alkali, extracting fish skin gelatin, performing enzymolysis on the fish skin gelatin by using neutral protease and trypsin, performing ultrafiltration, immobilized zinc ion affinity chromatography and reversed-phase high-performance liquid chromatography separation purification, thereby obtaining zinc phytochelatin Gly-Pro-Tyr-Gly-Pro-Phe-Gly-Pro-Trp-Gly (GPYGPFGPWG). ESI-MS testing shows that the molecular weight of thamnaconus modestus skin zinc phytochelatin is 1034.09Da.

Description

technical field [0001] The invention relates to a polypeptide of a processed aquatic product, in particular to a method for preparing a zinc chelating peptide from horse-faced puffer fish skin. Background technique [0002] Horse-faced fish, commonly known as rubber fish and skinned fish, belongs to the order Pufferiformes and the family Pufferidae. It is a common fish in my country. The skin of horse-faced puffer is thick and hard, and has no edible value. Most of them are thrown away, resulting in waste of resources and environmental pollution. Zinc is an essential trace element for the human body. Zinc deficiency in children can lead to poor growth and development, retarded mental development, and decreased immunity; zinc deficiency in pregnant women increases the rate of fetal malformation. Traditional zinc supplements, such as zinc sulfate and zinc gluconate, have low absorption and utilization rates and large gastrointestinal irritation, which is not conducive to long...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K7/06C07K1/36C07K1/34C07K1/22C07K1/20
Inventor 迟长凤王斌陈荫孙坤来
Owner 北京姿美堂生物技术股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com