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Application of a horse-faced puffer fish skin zinc chelating peptide

A technology of fish skin zinc and chelated peptides, applied in the preparation methods of peptides, peptides, specific peptides, etc., can solve problems such as blank research, and achieve the effects of easy digestion and absorption, safety, no toxic side effects, and strong zinc chelating ability.

Active Publication Date: 2020-08-04
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the applicant found that the research on the preparation of horse-faced fish skin zinc chelating peptide and its technology and product function using horse-faced fish skin as a raw material is blank

Method used

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  • Application of a horse-faced puffer fish skin zinc chelating peptide

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Experimental program
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Embodiment

[0020] 1) Preparation of horse-faced fish skin gelatin: green-finned horse-faced fish ( Navodon septentrionalis ) minced fish skin, added to 0.3% NaOH solution according to the ratio of solid to liquid 1 g:15 mL, stirred for 5 h, washed with double distilled water until neutral; then added to 0.4% H2O according to 1 g:10 mL 2 SO 4 soaked at room temperature for 2 days, and changed the water every 8 hours; finally, the treated fish skin homogenate was added to double distilled water according to the ratio of solid to liquid at 1:10, extracted dynamically at 70°C for 15 hours, and centrifuged at 6 000 g for 30 min, take the supernatant, and freeze-dry to obtain horse-faced puffer fish skin gelatin.

[0021] ) Enzymolysis of horse-faced puffer fish skin gelatin: Add horse-faced fish skin gelatin into barbiturate sodium-hydrochloric acid buffer according to the ratio of solid to liquid 1 g: 12 mL, adjust the pH value to 7.0, and store at 50 °C Incubate for 10 min, add neutral pr...

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Abstract

The invention relates to an application of a zinc chelating peptide of thamnaconus modestus fish skin for development of zinc-supplement medicines or functional products. The polypeptide is prepared by the following steps: with thamnaconus modestus fish skin as a raw material, removing non-collagen protein of the fish skin and mineral substances with acid and alkali; carrying out enzymolysis on fish skin gelatin employing neutral protease and trypsase; and carrying out ultrafiltration, immobilized zinc ion affinity chromatography and reversed-phase high performance liquid chromatography separation and purifying to obtain the zinc chelating peptide Gly-Pro-Tyr-Gly-Pro-Phe-Gly-Pro-Trp-Gly (GPYGPFGPWG). Through ESI-MS (electrospray ionization mass spectrometry) determination, the molecular weight is 1034.09Da.

Description

technical field [0001] The invention relates to the application of an aquatic product polypeptide, in particular to the application of a horse-faced puffer fish skin zinc chelating peptide. Background technique [0002] Horse-faced fish, commonly known as rubber fish and skinned fish, belongs to the order Pufferiformes and the family Pufferidae. It is a common fish in my country. The skin of horse-faced puffer is thick and hard, and has no edible value. Most of them are thrown away, resulting in waste of resources and environmental pollution. Zinc is an essential trace element for the human body. Zinc deficiency in children can lead to poor growth and development, retarded mental development, and decreased immunity; zinc deficiency in pregnant women increases the rate of fetal malformation. Traditional zinc supplements, such as zinc sulfate and zinc gluconate, have low absorption and utilization rates and large gastrointestinal irritation, which is not conducive to long-ter...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/22C07K1/20
Inventor 迟长凤王斌陈荫孙坤来
Owner ZHEJIANG OCEAN UNIV
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