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Method for preparing thamnaconus modestus skin zinc phytochelatin

A technology of pufferfish and fish skin zinc, which is applied in the field of peptides of processed aquatic products, can solve problems such as blanks, and achieve the effects of strong zinc chelating ability, safety, no toxic side effects, and easy digestion and absorption.

Active Publication Date: 2015-07-08
北京姿美堂生物技术股份有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the applicant found that the preparation of horse-faced fish skin zinc chelating peptide and its technology research using horse-faced fish skin as a raw material is in a blank

Method used

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  • Method for preparing thamnaconus modestus skin zinc phytochelatin

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Embodiment

[0019] 1) Preparation of horse-faced puffer fish skin gelatin: Greenfin horse-faced fish ( Navodon septentrionalis ) Fish skin was minced, added to 0.3% NaOH solution according to the ratio of solid to liquid 1 g:15 mL, stirred for 5 h, washed with double distilled water until neutral; then added to 0.4% H2O according to 1 g:10 mL 2 SO 4 soaked at room temperature for 2 days, and changed the water every 8 hours; finally, the treated fish skin homogenate was added to double distilled water according to the ratio of solid to liquid at 1:10, extracted dynamically at 70°C for 15 hours, and centrifuged at 6 000 g for 30 min, take the supernatant, and freeze-dry to obtain horse-faced puffer fish skin gelatin.

[0020] 2) Enzymatic hydrolysis of horse-faced puffer fish skin gelatin: Add horse-faced puffer fish skin gelatin into barbiturate sodium-hydrochloric acid buffer according to the ratio of solid to liquid: 1 g: 12 mL, adjust the pH value to 7.0, and keep it at 50°C for 10...

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Abstract

The invention relates to a method for preparing thamnaconus modestus skin zinc phytochelatin. The method specifically comprises the following steps: by taking thamnaconus modestus skin as a raw material, removing non-collagen and minerals in the fish skin by using acid and alkali, extracting fish skin gelatin, performing enzymolysis on the fish skin gelatin by using neutral protease and trypsin, performing ultrafiltration, immobilized zinc ion affinity chromatography and reversed-phase high-performance liquid chromatography separation purification, thereby obtaining zinc phytochelatin Gly-Pro-Tyr-Gly-Pro-Phe-Gly-Pro-Trp-Gly (GPYGPFGPWG). ESI-MS testing shows that the molecular weight of thamnaconus modestus skin zinc phytochelatin is 1034.09Da.

Description

technical field [0001] The invention relates to a polypeptide of a processed aquatic product, in particular to a method for preparing a zinc chelating peptide from horse-faced puffer fish skin. Background technique [0002] Horse-faced fish, commonly known as rubber fish and skinned fish, belongs to the order Pufferiformes and the family Pufferidae. It is a common fish in my country. The skin of horse-faced puffer is thick and hard, and has no edible value. Most of them are thrown away, resulting in waste of resources and environmental pollution. Zinc is an essential trace element for the human body. Zinc deficiency in children can lead to poor growth and development, retarded mental development, and decreased immunity; zinc deficiency in pregnant women increases the rate of fetal malformation. Traditional zinc supplements, such as zinc sulfate and zinc gluconate, have low absorption and utilization rates and large gastrointestinal irritation, which is not conducive to long...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K7/06C07K1/36C07K1/34C07K1/22C07K1/20
Inventor 迟长凤王斌陈荫孙坤来
Owner 北京姿美堂生物技术股份有限公司
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