Provided is a method for measuring seaweed
sugar concentration in biological matrix. The concentration measurement is achieved based on liquid chromatograghy-
mass spectrometry combined with pumping-inof auxiliary
reagent behind a column. The method comprises the steps that a to-be-measured sample is prepared, specifically, the biological matrix which is quantitatively absorbed is subjected to pretreatment through a
protein precipitation method and
protein depositions disturbing the measurement of the seaweed
sugar concentration are removed; quantitative analysis is carried out, specifically,a to-be-measured sample obtained through a previous treatment is transferred to the chromatograghic column, the auxiliary
reagent is pumped behind the column to carry out the liquid chromatograghy-
mass spectrometry analysis in a
mass spectrometer and a concentration result of nanogram level per milliliter is obtained. According to the method for measuring the seaweed
sugar concentration, the situation that the quantitative
lower limit of the seaweed sugar quantitative measurement decreases from a
microgram per milliliter level to 20 nanogram per milliliter by improving the process of the liquid chromatograghy-
mass spectrometry analysis is realized, the sensitivity is significantly improved, the simple and rapid
protein precipitation is used to proceed to the pretreatment,
processing stepsare apparently simplified,
processing time is shortened and the analysis rate is improved.