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157results about How to "The pretreatment method is simple" patented technology
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Liquid chromatogram-mass spectrum detection method of drugs and mental disease resistant drugs in blood
ActiveCN107807178AAchieving Simultaneous DetectionImprove accuracyComponent separationPretreatment methodTandem mass spectrometry
The invention provide a ultra-performance liquid chromatogram-mass spectrum detection method for 23 types of drugs and mental disease resistant drugs in blood. The method comprises the following steps: 1) sample processing: employing an organic solvent for processing a sample through protein sediment; 2) primary screening detection: employing a ultra-performance liquid chromatogram-tandem mass spectrum (UPLC-MS / MS) multi-reaction monitoring (MRM) mode for primary screening detection; and 3) employing the ultra-performance liquid chromatogram-tandem mass spectrum (UPLC-MS / MS) multi-reaction monitoring (MRM) mode for reinspection of the positive sample through the primary screening in the step 2). According to physical and chemical properties of 9 types of drugs and 14 types of mental disease resistant drugs, during reinspection, the UPLC-MS / MS detection condition which is different with the primary screening detection is employed, different sample pretreatment methods are employed, so that the detection method can satisfy the rapid, sensitive and accurate requirements of primary screening detection, and misjudgement of false positive results can be reduced.
Owner:中国民用航空局民用航空医学中心
Method of detecting amino acids in animal body fluid or tissue samples by ultrahigh efficient liquid chromatography-tandem quadrupole mass spectrometry
InactiveCN109633030AEasy to separateThe pretreatment method is simpleComponent separationQuadrupoleBiology
The invention discloses a method of detecting amino acids in animal body fluid or tissue samples by ultrahigh efficient liquid chromatography-tandem quadrupole mass spectrometry. In particular, afterthe animal body fluid or the tissue sample is extracted and processed, the sample is detected by ultrahigh efficient liquid chromatography-tandem quadrupole mass spectrometry, and the amino acids is analyzed qualitatively and quantitatively. The method of detecting amino acids in animal body fluid or tissue samples by ultrahigh efficient liquid chromatography-tandem quadrupole mass spectrometry can simultaneously detect 26 kinds of amino acids in samples such as the animal body fluid and the tissue. Multi-reaction monitoring mass spectrometry is used for qualitative and quantitative analysis.The instrumental detection time of each sample is only 25 minutes, which is about 25% of the time of the existing method. The preprocessing method is simple and does not need derivation. Every amino acid can be separated well by extracting selective ions.
Owner:江苏澳华生物科技研究院有限公司
Callicarpa nudiflora medicine, intermediate and fingerprint detection method and standard fingerprint of preparation
ActiveCN104215698AThe pretreatment method is simpleCharacteristic ingredients remain intactComponent separationClinical efficacyCallicarpa nudiflora
The invention discloses a callicarpa nudiflora medicine, an intermediate and a fingerprint detection method of a preparation. The method comprises the following steps: (a) preparation of a reference solution; (b) preparation of a test solution; (c) chromatographic condition; (d) formulation of a standard fingerprint of isoacteoside as a reference peak; and (e) quality control of the fingerprint. According to the above method, a pretreatment method of each tested object is simple, characteristic constituents are retained completely, stability is good, precision is high, and there is certain specificity. The separation effect of each characteristic peak in the fingerprint is good. The callicarpa nudiflora medicine, the intermediate and the fingerprint of the preparation have good correlation. The method can be used for identifying quality of the callicarpa nudiflora medicine and increasing production process controllability of the callicarpa nudiflora preparation, and is helpful for guaranteeing quality stability and clinical efficacy of the callicarpa nudiflora preparation. The invention also discloses the callicarpa nudiflora medicine obtained according to the above fingerprint detection method, the intermediate and the standard fingerprint of the preparation.
Owner:JIUZHITANG +2
Kit and method for quantitatively detecting five fat-soluble vitamins in blood plasma
PendingCN110763788AFast contentRapid quantitationComponent separationFluid phaseMass Spectrometry-Mass Spectrometry
The invention discloses a kit and method for quantitatively detecting five fat-soluble vitamins in blood plasma. The kit comprises a fat-soluble vitamin calibrator, an isotope mixed standard substance, a fat-soluble vitamin quality control substance, an instrument quality control substance and a mobile phase additive. According to the invention, a solid-phase support liquid-liquid extraction method is adopted and is matched with an automatic pipetting station, so a sample pretreatment method is greatly simplified, and the aim of simultaneously and accurately detecting the five fat-soluble vitamins at high flux and high speed is fulfilled by combining a liquid chromatography-tandem mass spectrometry method. According to the invention, during the detection of a peripheral blood plasma sample, five fat-soluble vitamins can be quantified at a time only by about 250 microliters of plasma sample; moreover, due to a fact that the pretreatment method is simple and quick, the operation time issaved, the content of the fat-soluble vitamins in a human body can be effectively diagnosed and detected according to the indexes, and diseases caused by lack or excess of the fat-soluble vitamins canbe prevented and diagnosed in time. Therefore, the kit and method have extremely high application values in clinical, health examination and scientific research.
Owner:SHENZHEN HUADA GENE INST
Method for detecting fish parvalbumin through liquid chromatography tandem mass spectrometry
ActiveCN108469495AEliminate the effects of detectionHigh purityComponent separationGas chromatography–mass spectrometryPeak area
The invention provides a method for detecting fish parvalbumin through liquid chromatography tandem mass spectrometry. The method comprises the following steps: step 1: choosing a characteristic peptide fragment; step 2: making a standard curve: taking the concentration value of a quantitative peptide fragment standard product as the x-coordinate and a quantitative daughter ion peak area as the y-coordinate to draw the standard curve, and obtaining an equation; step 3: processing and implementing enzymolysis on a to-be-detected fish meat sample to obtain a liquid supernatant, implementing HPLC-MRM-MS / MS detection on the liquid supernatant, obtaining the daughter ion peak area of the quantitative peptide fragment in the fish meat sample, resolving the concentration of the quantitative peptide fragment in the sample, and further obtaining the concentration of the parvalbumin in the sample. The invention aims at establishing a liquid chromatography tandem mass spectrometry method, and themethod has the advantages of accuracy, precision, sensitivity and the like.
Owner:OCEAN UNIV OF CHINA
Probiotics activity retention method and application thereof to solid-state fatty food
The invention discloses a probiotics activity retention method and application thereof to solid-state fatty food, and belongs to the technical field of food processing. According to the probiotics activity retention method, high-concentration glycerol is utilized for dehydration pretreatment of active probiotics by the osmotic equilibrium principle to achieve the purpose of reducing remaining moisture in probiotics. The obtained active bacterial sludge is added in the chocolate temperature-regulating technological process to form a favorable crystal structure together with chocolate fat, and time for probiotics to be active in chocolate is effectively prolonged, so that probiotic chocolate is obtained. The activity retention effect of probiotics in prepared chocolate is approximate to that of a product obtained through processing of freeze-dried bacterial powder. Through adoption of the probiotics activity retention method, a high cost caused by preparation of freeze-dried powder is avoided, energy consumption and equipment investment are low, and the processing cost can be greatly reduced.
Owner:JIANGNAN UNIV
Fluorescence spectrum detection device for pesticide residue
InactiveCN103424387AThe pretreatment method is simpleFast pretreatment methodFluorescence/phosphorescenceIntegratorSmall sample
The invention discloses a fluorescence spectrum detection device for pesticide residue, which comprises a high-brightness LED module; a dodging integrator rod is arranged in front of the high-brightness LED module; a drive circuit is arranged behind the high-brightness LED module; a focusing lens is arranged in front of the dodging integrator rod; a vegetable stem leaf is placed near the focusing lens; a coupling lens is arranged on the other side of the vegetable stem leaf; a light filter pack and a light sensitive element are sequentially arranged at the back of the coupling lens. Through the adoption of the technical scheme, a novel narrow-band multispectral LED light source is adopted, the emission of a plurality of narrow-band light wave lengths can be achieved through a single light source module, the broadband narrow-band light covering achieved through the combination of a plurality of light sources traditionally is achieved, the system cost is lowered greatly, and the device has the advantages of high sensitivity, small sample use amount, quickness, good reproducibility and no destructibility, and has a wide application prospect in the aspect of detecting trace substances, especially pesticide residue.
Owner:SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
Evaluation method for cell metabolism toxicity of organic pollutants
InactiveCN105021716AThe pretreatment method is simpleEasy to operateComponent separationHepg2 cellsHigh flux
The invention provides a method which takes HepG2 cells as testing cells and carries out an exposure treatment experiment of pollutants. The method comprises the following steps: (a) establishing a pre-treatment method for rapidly extracting intracellular and extracellular metabolic products; (b) carrying out panoramic analysis and target analysis on the metabolic products of the cells by using a UPLC-QTOF and HPLC-MS / MS; (c) classifying and screening high-flux metabolites by statistical analysis; and (d) analyzing a high-attention metabolism path of organic pollutant influenced cells by combining a metabolism flow analysis system and a path analysis technology. The pre-treatment method is simple; the UPLC-QTOF is combined with the HPLC-MS / MS so that the intracellular and extracellular metabolic products of the cells can be effectively analyzed; and the high-attention metabolism path of the organic pollutant influenced cells can be directly analyzed by combining statistical treatment, metabolism flow analysis and metabolism path analysis; and the method has the advantages of simplicity in operation, short analysis time, good repeatability, capability of acquiring comprehensive information quantity and the like.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Chemiluminescent enzyme-linked immunosorbent assay kit of tenuazonic acid and application method thereof
InactiveCN103487575AHigh precisionImprove stabilityChemiluminescene/bioluminescenceBiological material analysisAntigenTenuazonic acid
The invention discloses a chemiluminescent enzyme-linked immunosorbent assay kit of a tenuazonic acid and an application method thereof, and belongs to the technical field of chemiluminescent enzyme-linked immunosorbent assay. An indirect competition method is adopted by the assay kit; the kit comprises a chemiluminescent ELISA (Enzyme-Linked Immuno-Sorbent Assay) plate coated with tenuazonic acid antigens, a tenuazonic acid standard substance, tenuazonic acid antibodies, enzyme-labeled antibodies, a chemiluminescent liquid and a washing liquid. The application method of the kit comprises the following steps: (1) pretreating a sample to be detected; (2) orderly adding the solution or sample of the tenuazonic acid standard substance and the tenuazonic acid antibodies, and adding the enzyme-labeled antibodies after a competing reaction, and finally adding the chemiluminescent liquid to carry out the quantitative determination of the tenuazonic acid through a chemiluminescent immunoassay analyzer; (3) processing and analyzing the results. The chemiluminescent enzyme-linked immunosorbent assay kit of the tenuazonic acid provided by the invention is high in sensitivity, good in stability and suitable for screening of lots of samples, and thus has enormous practical application and popularization significance.
Owner:SOUTH CHINA AGRI UNIV
Method for detecting five sweetening agents in tobacco essence through ultra-high performance liquid chromatography-tandem mass spectrometry
ActiveCN108872448AGood effectStrong anti-matrix interference abilityComponent separationFood additiveRepeatability
The invention belongs to the technical field of physical and chemical inspection of food additive residues in tobacco essences, and particularly relates to a method for detecting five sweetening agents in a tobacco essence through the ultra-high performance liquid chromatography-tandem mass spectrometry. The method is characterized by comprising the following steps that the tobacco essence is weighed, then ultrapure water is added, then oscillating extraction is carried out, extract liquid is purified and filtered and is diluted by using the ultra-pure water, and then the sweetening agents inthe tobacco essence are directly detected through the ultra-high performance liquid chromatography-tandem mass spectrometry. The method has the advantages of being capable of rapidly and accurately detecting the residual quantity of the sweetening agents in the tobacco essence, environmentally friendly, accurate in result, less in interference, high in sensitivity and good in repeatability.
Owner:CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
Bifenthrin antigen, antibody and uses thereof
InactiveCN101135683ADepends on affinityDepends on detectabilityOrganic chemistryBiological testingBenzoic acidMetabolite
The method comprises: synthesizing the hapten 2-methyl-3-phenyl benzyl succinic acid ester (for short: LBC) and the 2-methyl-3-phenylbenzene benzoic acid (for short: LBY); coupling the LBc with protein to prepare the artificial immunity antigen and immunity rabbit to the specificity polyclonal antibody of Bifenthrin; coupling the LBy with the protein to prepare the envelope antigen; using the synthesized envelope antigen and specificity antibody to establish the enzyme immunoassay EIA analysis method; the detection range of the Bifenthrin can reach 0.016mg / L, and the linear range of it is 0.01-50mg / kg.
Owner:NANJING AGRICULTURAL UNIVERSITY
Detecting method for hexavalent chromium in gelatin and products thereof
ActiveCN102841090AEasy to handleLow costMaterial analysis by observing effect on chemical indicatorAlkalinityPhosphate
The invention discloses a detecting method for hexavalent chromium in gelatin and products thereof, particularly relates to the detecting method for the hexavalent chromium in colored gelatin products. The method includes the following steps: using phosphate buffered solution to extract; adjusting PH value of the extraction solution to >=12.5; directly adding graphitized carbon black powder in the extraction solution with colorant for purification and decoloration; in acid medium, hexavalent chromium oxidation diphenyl carbonyl two hydrazine forms soluble amaranthine compounds. The ultraviolet spectrophotometry method is adopted to detect the content of the hexavalent chromium. The detecting method has the advantages that under the condition of highly alkalinity, the recovery rate of the hexavalent chromium can be guaranteed. Moreover, the graphitized carbon black powder can carry out sufficient decoloration for pigment in samples and avoid color interference. The detecting method is high in recovery rate, simple in pretreatment, low in cost, quick and accurate in result.
Owner:青岛谱尼测试有限公司
Detection method for simultaneously measuring OXC in human plasma and metabolite MHD and MHD-G
InactiveCN105136957AThe pretreatment method is simpleSuitable for routine testingComponent separationMetaboliteAnalysis study
The invention relates to a detection method for simultaneously measuring OXC in human plasma and metabolite MHD and MHD-G, and belongs to the technical field of biological detection. An experiment is carried out by taking nitrazepam as an internal standard, and can simultaneously detect the plasma concentrations of the three compounds by adopting high performance liquid chromatography-tandem mass spectrometry after pretreatment through precipitation protein. The detection method is short in time, high in specificity and sensitivity, and convenient in operation, and is successfully applied to the analysis and research of oxcarbazepine in the human plasma and the metabolite thereof.
Owner:WUXI PEOPLES HOSPITAL
Radix astragali medicinal materials, intermediate body and method for testing fingerprint of formulation thereof as well as standard fingerprint
ActiveCN101327246AImprove controllabilityThe pretreatment method is simpleComponent separationMetabolism disorderDrugChemistry
The invention discloses a fingerprint map detecting method of radix astragali drug, intermediate compound and a preparation thereof, the method is as follows: taking onocol as reference substance, obtaining each sample solution through the processes of extracting / dissolving, determining constant volume and the like, performing HPLC detection under the condition of the same chromatogram, performing gradient elution, recording a chromatogram map, and obtaining the product. In the method, the preliminary treatment method for each sample is simple, the reservation of the characteristic components is complete, the sample solution is stable; the precession is high, reproduction quality is good, and the solution has a certain specificity; the separation effect of each characteristic peak in the obtained fingerprint map is good, the fingerprint map of the radix astragali drug, intermediate compound and the preparation has better correlation; the method can be used for identifying the fake of the radix astragali drug, increases the controllability of the production process of the radix astragali preparation, and is in favour of ensuring the quality stability and the clinical healing efficacy of the radix astragali preparation. The invention also discloses a standard fingerprint map of radix astragali drug, intermediate compound and preparation thereof obtained by the fingerprint map detecting method.
Owner:SICHUAN BAILI PHARM CO LTD
Measurement method for residual amount of pentachlorophenol in cigarette paper
ActiveCN103323548AIncrease contactImprove extraction efficiencyComponent separationPentachlorophenolBiochemical engineering
Owner:CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
Method for detecting content of urea in brewed wine by using high performance liquid chromatography
InactiveCN103760266AThe pretreatment method is simpleEasy to handleComponent separationUrea derivativesFluorescence
The invention relates to a method for detecting the content of urea in brewed wine by using high performance liquid chromatography. The method comprises the following steps: weighing an appropriate sample, selecting a proper solvent, and performing pre-treatment on the sample by using precolumn derivatization, rotary evaporation concentration and the like; and detecting urea derivatives by using a high performance liquid chromatograph connected with a fluorescence detector, wherein the organic flow phase and the inorganic flow phase are in variable ratio, the flow speed is set as 1mL / min, the sampling amount is set as 10 microliters, the chromatographic column is selected as the C18 chromatographic column, the column temperature is 40-50 DEG C, and the detection wavelength of the fluorescence detector as follows: the lambda ex is 208nm, and the lambda em is 308nm. The detection method provided by the invention has the characteristics of being simple, fast and high in recycling rate.
Owner:CENT TESTING INT GRP CO LTD
Method for detecting five sweetening agents in electronic cigarette liquor through ultra-high performance liquid chromatography-tandem mass spectrum
ActiveCN108956840AGood effectStrong anti-matrix interference abilityComponent separationFood additiveElectron
The invention belongs to the technical field of physical and chemical inspection for food additive residues in electronic cigarette liquor and specifically relates to a method for detecting five sweetening agents in electronic cigarette liquor through ultra-high performance liquid chromatography-tandem mass spectrum. The method is characterized by comprising the following steps: first, weighting the electronic cigarette liquor, and then adding ultrapure water for diluting, and at last, determining the sweetening agents in the electronic cigarette liquor through ultra-high performance liquid chromatography-tandem mass spectrum. According to the method, the sweetening agent residues in electronic cigarette liquor can be quickly and accurately detected. The invention has the advantages of environment-friendly method, accurate result, little interference, high sensitivity and good repeatability.
Owner:CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
Flos lonicerae and radix scutellariae lung-clearing preparation fingerprint spectrum establishment method and standard fingerprint spectrum and application thereof
ActiveCN104792889AThe pretreatment method is simpleEasy to separateComponent separationFlosClinical efficacy
The invention discloses a flos lonicerae and radix scutellariae lung-clearing preparation fingerprint spectrum establishment method and a standard fingerprint spectrum and application thereof. According to the method, pretreatment methods of various test samples are simple, characteristic ingredients can be retained completely, product quality information can be comprehensively reflected, the fingerprint spectrum has strong specificity and stability, and high precision, separation effect of each characteristic peak in the obtained fingerprint spectrum is good; the fingerprint spectrum can be used to quickly determine whether a flos lonicerae and radix scutellariae lung-clearing preparation is qualified so as to ensure the stable quality and the clinical curative effect of the flos lonicerae and radix scutellariae lung-clearing preparation.
Owner:湖南安邦制药股份有限公司
Construction method for fructus evodiae medicinal material characteristic spectrum, and fructus evodiae medicinal material quality detection method
The invention belongs to the technical field of Traditional Chinese medicines, and particularly relates to a construction method for fructus evodiae medicinal material characteristic spectrum, and a fructus evodiae medicinal material quality detection method. The construction method comprises the following steps that: preparing the solution of a sample for testing, preparing reference substance solution, detecting a high performance liquid chromatography, and establishing a fructus evodiae medicinal material reference characteristic spectrum. By use of the fructus evodiae medicinal material characteristic spectrum established with the construction method, the characteristics of the chemical ingredients of fructus evodiae medicinal materials can be fully displayed, a characteristic peak information amount is rich, reproducibility is good, the characteristic spectrum is accurate and reliable, the fructus evodiae medicinal material and relevant fake products can be comprehensively and effectively identified, and the quality of the fructus evodiae medicinal materials can be effectively monitored.
Owner:湖南省中医药研究院
Method for determining major sweetening agents in edible essence and flavor by HPLC-MS/MS
ActiveCN110824064ANo pollution in the processEliminate the effects ofComponent separationSucroseSolvent
This inventio discloses a method for determining major sweetening agents in an edible essence and flavor by HPLC-MS / MS. The method comprises the steps of preparing a matrix solvent, preparing a seriesof standard working solutions, preparing a sample solution, performing HPLC-MS / MS analysis, building a standard work curve, and performing sample result calculation. According to the method, curves are prepared and corrected by the matrix solvent for the first time; and an HPLC-MS / MS chromatograph is used for simultaneously determining nine kinds of major sweetening agents such as xylitol, acesulfame potassium, saccharin sodium, sodium cyclamate, sucralose, aspartame, neohesperidin, neotame and stevioside in edible essence. The method has the advantages that the proper matrix solvent is prepared, and is used for preparing a standard work curve; the influence caused by matrix effects on the quantification of target materials in the mass spectrometry ionization process is eliminated; the contents of the major sweetening agents in the edible essence can be simultaneously detected; the result is accurate; the interference is little; the sensitivity is high; and the repeatability is high,and the like.
Owner:CHINA TOBACCO JIANGSU INDAL
Method for monitoring environmental element pollution by transplanting fresh water bivalves
InactiveCN101539538ASame background levelEnvironmental features are clearMaterial analysis by electric/magnetic meansParenchymaFresh water organism
The invention relates to a method for monitoring environmental element pollution by transplanting fresh water bivalves, which adopts the following steps: using pond culturing mussels as a comparison population, and taking mussels with same-batch and consistent specification transplanted to a water area to be monitored as an experimental population; temporarily culturing living mussels in aerated water to remove impurities in shells and alimentary tracts, removing the shells to obtain mussel parenchyma, storing the mussel parenchyma in a refrigerator; taking the mussel parenchyma out, washing the mussel parenchyma by using ultra-pure water after unfreezing, and drying, grinding the mussel parenchyma into powder for being placed in a dryer for standby application; taking the dried mussel parenchyma, placing the dried mussel parenchyma into a container, adding high-pure nitric acid, and acid-splitting the dried mussel parenchyma under room temperature; digesting by a microwave oven, and performing the constant volume to solution to be monitored by using ultra-pure water; and measuring and indicating the element accumulation discrepancy of shellfishes-mussels parenchyma and the content of heavy metal pollutant and the like as well as the experiment monitoring effect by utilizing an inductively-coupled plasma spectrometer. The invention can more accurately reflect the difference of multiple heavy metals and trace elements in shellfishes in changeable water environment on pollution degree and accumulation level.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
Method for determining 1,1,1-trimethylolpropane in tobacco paper
ActiveCN104122345AEasy to handleEasy processing conditionsComponent separationHazardous substanceProcess engineering
The invention belongs to the technical field of physical and chemical inspection of hazardous substances remained in tobacco paper, and in particular relates to a method for determining 1,1,1-trimethylolpropane in tobacco paper. The method is characterized by comprising the steps: cutting the tobacco paper into pieces, adding ultrapure water for carrying out ultrasonic extraction, and directly determining the 1,1,1-trimethylolpropane in the tobacco paper by adopting a liquid chromatogram- tandem mass spectrum after an extraction solution is centrifuged. The method can rapidly and accurately detect the residual amount of the 1,1,1-trimethylolpropane in the paper and has the advantages of being accurate in determination result, low in determining interference, high in sensitivity and good in repeatability.
Owner:CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
UPLC fingerprint spectrum detection method of lamiophlomis rotata (Benth.)Kudo and preparations thereof
InactiveCN109975439AThe pretreatment method is simpleCharacteristic ingredients remain intactComponent separationHplc methodChlorogenic acid
The invention discloses a UPLC fingerprint spectrum detection method of lamiophlomis rotata (Benth.)Kudo and preparations thereof. The method includes the following steps of: (a) the preparation of areference substance solution; (b) the preparation of a test solution; (c) the provision of UPLC detection conditions; (d) the formation of a standard fingerprint spectrum with sesamoside, caffeic acid, chlorogenic acid, shanzhiside methyl ester, loganin, 8-O-acetyl shanzhiside methyl ester, forsythiaside B, galuteolin, verbascoside, Isoacteoside and the like adopted as reference peaks; and (e) thequality control of the fingerprint spectrum. According to the method, during test solution preparation, an extraction solvent is environment-friendly. The method is simple. Compared with a traditional HPLC method, the method is more convenient and rapider, and save energy and reduce energy consumption. The separation effect of the characteristic peaks of the fingerprint spectrum of the lamiophlomis rotata (Benth.)Kudo and the preparation thereof is good; and characteristic compositions and medicinal compositions can be reserved completely. The method has the advantages of good repeatability,high stability, high precision and strong specificity. The method can accurately and quickly control the lamiophlomis rotata (Benth.)Kudo and the preparations thereof.
Owner:JIUZHITANG
Method for measuring D-sorbitol in plasma or urine
InactiveCN102621267AThe pretreatment method is simpleSimple methodComponent separationOrganic solventD-Sorbitol
The invention discloses a method for measuring D-sorbitol in plasma or urine with the liquid chromatography-mass spectrometry, which comprises the following steps: taking a to-be-tested sample, adding a certain amount of an organic solvent protein precipitation for protein precipitation, separating via a chromatographic column after pretreatment, and detecting with a mass spectrometric detector. The method disclosed by the invention is rapid, accurate, high in sensitivity and convenient in operation, and is suitable for measuring concentration of D-sorbitol in plasma and urine.
Owner:JIANGSU PROVINCIAL HOSPITAL OF TCM
Method for determining concentration of tadalafil in blood plasma by liquid chromatography-mass spectrometry
InactiveCN110927307AThe pretreatment method is simpleGood peak shapeComponent separationChemistryChromatography column
The invention discloses a method for determining the concentration of tadalafil in blood plasma by liquid chromatography-mass spectrometry, which adopts a liquid chromatography-mass spectrometry system for determination and comprises the following steps: taking a sample to be detected, adding a certain amount of mixed organic solvent for extraction and pretreatment, performing separating by usinga chromatographic column, and performing detecting by using a mass spectrometry detector. The method disclosed by the invention is rapid, accurate, high in sensitivity and simple and convenient to operate, and provides a basis for measuring the blood concentration of tadalafil; according to the method, the linear range of a plasma standard curve is 1-600ng / mL, the intra-batch precision RSD and theinter-batch precision RSD are both less than + / -15%, and the method is suitable for measuring the concentration of tadalafil in plasma.
Owner:徐州立兴佳正医药科技有限公司
Pretreatment method for base rubber of single-component dealcoholized type room temperature vulcanized silicone rubber
InactiveCN105237783AThe pretreatment method is simpleEfficient pretreatment methodPolymer scienceVulcanization
The invention relates to a pretreatment method for base rubber of single-component dealcoholized type room temperature vulcanized silicone rubber, and belongs to the field of dealcoholized type room temperature vulcanized silicone rubber. The pretreatment method comprises the following steps that a catalytic reaction is carried out, wherein the base rubber with the viscosity ranging from 10,000 mPa.s to 60,000 mPa.s is fed into a reaction still with a stirring device, and a catalyst and a cross-linking agent are sequentially added; a neutral reaction is carried out, wherein after the reaction is finished, neutralizer accounting for 0.2-10 parts by mass is added for the neutral reaction; a devolatiligation process is carried out, wherein after neutralization, low components generated in the reaction are all removed in the water removal process of the base rubber. 107 rubber treated through the steps is subjected to 1HNMR, 29Si-NMR and IR analysis and contains no silicon hydroxyl. No obvious rising phenomenon occurs when the treated 107 rubber is used as raw materials for preparing the single-component dealcoholized type room temperature vulcanized silicone rubber. Under the condition of accelerated aging, surface drying after placing, curing time, extrusion and the like have no obvious changes.
Owner:TANGSHAN SANYOU SILICON IND
Method for determining concentration of carbamazepine in plasma by liquid chromatography mass spectrometry
InactiveCN109541107AThe pretreatment method is simpleSuitable for routine determinationComponent separationOrganic solventGas chromatography–mass spectrometry
The invention discloses a method for determining concentration of carbamazepine in plasma by liquid chromatography mass spectrometry. A liquid chromatography mass spectrometry system is used for determining. A sample to be determined is added with a certain amount of mixed organic solvent to extract twice, is separated through a chromatographic column after pretreatment, and is detected by a massspectrometer. The method is rapid, accurate, high in sensitivity, and simple and convenient to operate. The method provides a basis for the blood drug concentration determination of carbamazepine. Thelinear range of a plasma standard curve in the method is 5 to 1000 ng / mL. The intra-batch and inter-batch precision RSD are less than + / -15%. The method is suitable for determining the concentrationof carbamazepine in the plasma.
Owner:徐州立顺康达医药科技有限公司
Method for detecting pesticide residue quantity in radix ophiopogonis
InactiveCN108387655AHigh penetration rateReduce use costComponent separationReference productNitrogen
The invention relates to a method for detecting the pesticide residue quantity in radix ophiopogonis. The method comprises the following steps of (1) weighing radix ophiopogonis to be tested; performing treatment by using a QuEChERS pretreatment technology; preparing a first test product solution; (2) weighing a proper amount of ribonolactone and sorbitol; adding acetonitrile and water for dissolution; using the materials as an analysis protection agent; (3) measuring the first test product solution; blowing the solution to the near dry state by nitrogen; adding acetone for dissolution; addingthe analysis protection agent; performing uniform mixing; using the materials as a second test product solution; (4) weighing a pesticide reference product solution; respectively adding acetonitrileto prepare a first reference product solution; measuring and taking the first reference product solution; respectively adding acetone to prepare a second reference product solution; (5) using a testing method; (6) calculating the content of pesticide in the first test product solution and the second test product solution according to an outer standard method. The method has the characteristics ofhigh repeatability, high accuracy, low utilization cost and the like. The pesticide residue quantity in the radix ophiopogonis can be fast, effectively, simply and conveniently detected.
Owner:四川省食品药品检验检测院
Method for determining concentration of 5'-methoxyl-3',4'-methylenedioxyphenyl cinnamic acid isobutyl amide in plasma
InactiveCN104076116AThe pretreatment method is simpleHigh sensitivityComponent separationChemistryChromatography column
The invention discloses a met / hod for determining the concentration of 5'-methoxyl-3',4'-methylenedioxyphenyl cinnamic acid isobutyl amide in plasma. A liquid chromatography-mass system is adopted to determine. The method comprises the following steps: taking a to-be-determined sample first, adding a certain quantity of organic solvent for extraction, separating through a chromatographic column after pretreatment, and determining by using a mass spectrometry detector. The method disclosed by the invention has the advantages of rapidity, accuracy, high sensitivity, simplicity and convenience in operation and is suitable for determining the concentration of 5'-methoxyl-3',4'-methylenedioxyphenyl cinnamic acid isobutyl amide in plasma.
Owner:JIANGSU PROVINCIAL HOSPITAL OF TCM
Method for measuring three kinds of brevetoxins in shellfishes through liquid chromatography tandem mass spectrometry
InactiveCN109001333AHigh recovery rateGood repeatabilityComponent separationEcological environmentGas chromatography–mass spectrometry
The invention belongs to the technical field of toxin detection, and discloses a method for measuring three kinds of brevetoxins in shellfishes through a liquid chromatography tandem mass spectrometry. Pre-processing steps are optimized from the four aspects of an extraction reagent, a solid-phase extraction column, sampling solution and eluant; and a high-efficiency liquid chromatography-tandem mass spectrometry detection method of brevetoxins in shellfishes is established. A pre-processing method in the invention is simple, high in sensitivity and good in stability, and suitable for simultaneous measurement of three kinds of brevetoxins in shellfishes; the detection method in the invention is used for quantitatively analyzing three kinds of brevetoxins in shellfishes through the liquid chromatography tandem mass spectrometry; the method is high in recovery rate and good in repeatability; the quantitation limit is up to 3.0 mu g / kg; the method is steady, reliable and high in sensitivity; current analysis detection requirements of brevetoxins are satisfied; the method has popularization and application values; and thus, marine ecological environment monitoring and survey tracking of a pollution source are easily carried out.
Owner:MARINE FISHERIES RES INST OF ZHEJIANG
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