Method for monitoring environmental element pollution by transplanting fresh water bivalves

Abstract

The invention relates to a method for monitoring environmental element pollution by transplanting fresh water bivalves, which adopts the following steps: using pond culturing mussels as a comparison population, and taking mussels with same-batch and consistent specification transplanted to a water area to be monitored as an experimental population; temporarily culturing living mussels in aerated water to remove impurities in shells and alimentary tracts, removing the shells to obtain mussel parenchyma, storing the mussel parenchyma in a refrigerator; taking the mussel parenchyma out, washing the mussel parenchyma by using ultra-pure water after unfreezing, and drying, grinding the mussel parenchyma into powder for being placed in a dryer for standby application; taking the dried mussel parenchyma, placing the dried mussel parenchyma into a container, adding high-pure nitric acid, and acid-splitting the dried mussel parenchyma under room temperature; digesting by a microwave oven, and performing the constant volume to solution to be monitored by using ultra-pure water; and measuring and indicating the element accumulation discrepancy of shellfishes-mussels parenchyma and the content of heavy metal pollutant and the like as well as the experiment monitoring effect by utilizing an inductively-coupled plasma spectrometer. The invention can more accurately reflect the difference of multiple heavy metals and trace elements in shellfishes in changeable water environment on pollution degree and accumulation level.

Description

technical field [0001] The invention relates to a method for monitoring environmental element pollution by transplanting freshwater bivalve molluscs, specifically selecting freshwater bivalve molluscs that have been bred under artificial culture pond conditions and having consistent growth specifications and transplanting them into the natural environment to be tested. Trial breeding in waters, by comparing and measuring the contents of various metal elements in two types of mussel soft tissues, and then monitoring the differences in the accumulation of various heavy metal pollution and trace elements in shell bodies in different environments, belongs to the field of new technology development for environmental monitoring. Background technique [0002] At present, the ecological environment of my country's inland waters is mainly evaluated by measuring the concentration of water pollutants. Because the concentration of pollutants in water is generally very low and easy to ch...

AI Technical Summary

Problems solved by technology

However, because field sampling may be limited by many factors (such as the number of samples is uncertain, the size is uneven, some samples cannot be collected in some waters to be investigated, and the germplasm quality of the samples may vary, etc.), there is an urgent need for in-situ monitoring in the field. On the basis of this, we have developed a standardized monitoring indicator organism that can be transplanted and recovered to investigate heavy metal pollution in various freshwater waters, in order to adapt to the "national" The target requirements of "establishing a technical system for water pollution monitoring, control and water environment quality improvement suitable for national conditions" proposed in the Eleventh Five-Year "Science and Technology Development Plan"

[0004] In the field of biological monitoring environment, bivalve mollusc colonization monitoring is still a brand-new technology, and there is no precedent for standardized colonization monitoring in freshwater environment