59results about How to "Strong anti-matrix interference ability" patented technology

The invention relates to a method for detecting mercury ion residue of a fluorescent signal conversion mechanism based on a nucleic acid aptamer structure. In the method, a mercury ion nucleic acid aptamer marked with a fluorescein molecule, and a complementary sequence marked with a quenching element are utilized to form a fluorescent detection system, wherein the mercury ion nucleic acid aptamer is a stem loop structure composed of 27-28 bases and owns a stem formed by covalent binding of the bases GGAC and GTCC; and the base sequence of the complementary sequence Q2 is shown in SEQ ID NO.1. The method for detecting the mercury ion residue by the fluorescent detection system comprises the following steps of: adding mercury ions with a serial concentration and a complementary sequence Q2 competitive binding nucleic acid aptamer; establishing a standard curve according to the change of fluorescent signals and determining the lowest detection limit and linear range; and then, carrying out marking detection on a sample to be detected and judging the content of the mercury ions in the sample according to the standard curve. The method disclosed by the invention has the advantages of rapidness, simplicity, high sensitivity and selectivity and less sample quantity demand, and is applicable to the detection of the mercury ions in the actual sample.

The invention discloses a methyl mercury visual detection method of gold-mercury alloy nanoparticle growth regulation and control based on a DNA template, and belongs to the technical field of heavy metal detection. According to the method, an HT9 DNA nucleic acid template is uniformly mixed with different concentrations of methyl mercury standard solutions, incubation is carried out for 5 minutesafter mixing, a sodium borohydride solution is added to prepare nano seed crystal by reduction, a hydroxylamine hydrochloride solution and a chloroauric acid solution are sequentially added then, a solution color is recorded after fully and uniformly mixing, a standard color card is established, the absorbance of the solution is determined by a microplate reader and corresponds to the concentration of methyl mercury, and a standard work curve is established. Operation is carried out by replacing the methyl mercury standard solution with a marine product extracting solution, a corresponding solution color is recorded and compared with the standard color card, the absorbance of the solution is detected and substituted into the standard curve, so that organic mercury in a marine product is detected. The method is simple, sensitive, stable and high in anti-interference capability, does not need heating, can be used to complete detection within 30 minutes, and is low in detection cost.

The invention relates to a heterogeneous photocatalysis resonance fluorescence method for accurately detecting trace uranium in an environmental water sample. The method mainly comprises the following steps: quantitatively separating the trace uranyl in a complex environmental water sample by using solid phase extraction particles UMIP having a specific recognition and adsorptive action on UO2<2+>, and adsorbing the trace uranyl to the surface of the UMIP to form a uranium-containing molecularly imprinted polymer; then accurately detecting the content of the uranium by the heterogeneous photocatalysis resonance fluorescence method. The method has the characteristics of high sensitivity, high specificity, high matrix interference resistance, simplicity, quickness and low cost. The invention also discloses a heterogeneous photocatalysis kinetic analysis and detection device which comprises a photocatalytic reaction system, a low-heat high light source, an air-cooled constant temperature system and an electromagnetic-stirring system.

The invention belongs to the technical field of physical and chemical inspection of food additive residues in tobacco essences, and particularly relates to a method for detecting five sweetening agents in a tobacco essence through the ultra-high performance liquid chromatography-tandem mass spectrometry. The method is characterized by comprising the following steps that the tobacco essence is weighed, then ultrapure water is added, then oscillating extraction is carried out, extract liquid is purified and filtered and is diluted by using the ultra-pure water, and then the sweetening agents inthe tobacco essence are directly detected through the ultra-high performance liquid chromatography-tandem mass spectrometry. The method has the advantages of being capable of rapidly and accurately detecting the residual quantity of the sweetening agents in the tobacco essence, environmentally friendly, accurate in result, less in interference, high in sensitivity and good in repeatability.

The invention belongs to the field of solid phase extraction, in particular to a PS / SiO 2 @PDA composite nanofiber online micro solid phase extraction column and its preparation method. The online micro-solid phase extraction column includes a pre-column column cover, a pre-column core filled with an adsorbent material and a manual six-way valve, and the adsorbent material is PS / SiO 2 @PDA composite nanofibers, the PS / SiO 2 @PDA composite nanofiber is prepared by PS / SiO 2 Electrospun materials, and PS / SiO 2 The electrospun material was placed in dopamine salt solution to make dopamine in PS / SiO 2 Surface self-polymerization of electrospun materials to form PS / SiO 2 @PDA composite nanofibers. The present invention combines PS / SiO 2 @PDA composite nanofiber micro-solid phase extraction column combined with online extraction technology has a high degree of automation, easy operation, fewer sources of error, and high sensitivity. It is suitable for sample pretreatment of trace analytes in complex matrices.