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A method for visual detection of methylmercury based on DNA regulation of gold-amalgam growth

A detection method and methylmercury technology are applied in the field of heavy metal detection to achieve the effects of good selectivity, low price and simple operation

Active Publication Date: 2020-12-29
FUJIAN AGRI & FORESTRY UNIV +1
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  • Application Information

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Problems solved by technology

On this basis, Fu Fengfu's research group designed a silver-mercury nano-alloy growth method to realize the visual detection of methylmercury and ethylmercury. The detection can only be completed by heating the reaction, which is limited by the heating equipment to a certain extent (Anal. Chem., 2018, 90, 8, 5489-5495)

Method used

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  • A method for visual detection of methylmercury based on DNA regulation of gold-amalgam growth
  • A method for visual detection of methylmercury based on DNA regulation of gold-amalgam growth
  • A method for visual detection of methylmercury based on DNA regulation of gold-amalgam growth

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Embodiment 1

[0034] A method for visually detecting methylmercury based on DNA regulation of gold-mercury growth, comprising the following steps:

[0035] (1) Establishment of comparison standards:

[0036] Take 50 μL of methyl mercury standard solution with a series of different concentrations (0 μM, 0.05 μM, 0.1 μM, 0.2 μM, 0.5 μM, 1.0 μM, 2.0 μM, 3.0 μM, 4.0 μM) and 10 μL, 37.5 μM HT9 DNA template After mixing, place it at room temperature (25°C) for 5 minutes, then add 5 μL of 25mM sodium borohydride solution, mix well, centrifuge for 10 seconds, and stand at room temperature (25°C) for 20 minutes to prepare nano-crystal seeds ( figure 1 a), then add 5 μL of 200 mM hydroxylamine hydrochloride solution and 50 μL of 1.0 mM chloroauric acid HAuCl respectively 4 solution, after fully mixing for 1 minute, the gold-mercury nano-alloy product ( figure 1 b). Use a smart phone or a digital camera to record the color change of the solution (as the concentration of methylmercury increases, th...

Embodiment 2

[0043] Example 2 Specific detection of a methylmercury visual detection method based on DNA regulation of gold-mercury growth

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Abstract

The invention discloses a methyl mercury visual detection method of gold-mercury alloy nanoparticle growth regulation and control based on a DNA template, and belongs to the technical field of heavy metal detection. According to the method, an HT9 DNA nucleic acid template is uniformly mixed with different concentrations of methyl mercury standard solutions, incubation is carried out for 5 minutesafter mixing, a sodium borohydride solution is added to prepare nano seed crystal by reduction, a hydroxylamine hydrochloride solution and a chloroauric acid solution are sequentially added then, a solution color is recorded after fully and uniformly mixing, a standard color card is established, the absorbance of the solution is determined by a microplate reader and corresponds to the concentration of methyl mercury, and a standard work curve is established. Operation is carried out by replacing the methyl mercury standard solution with a marine product extracting solution, a corresponding solution color is recorded and compared with the standard color card, the absorbance of the solution is detected and substituted into the standard curve, so that organic mercury in a marine product is detected. The method is simple, sensitive, stable and high in anti-interference capability, does not need heating, can be used to complete detection within 30 minutes, and is low in detection cost.

Description

technical field [0001] The invention belongs to the technical field of heavy metal detection, and in particular relates to a methylmercury visual detection method for regulating the growth of gold-amalgam nanoparticles based on DNA templates. Background technique [0002] Mercury pollution is an environmental problem that cannot be ignored. Mercury in nature can be divided into non-based mercury and organic mercury, among which organic mercury contains methylmercury and ethylmercury. The main form of mercury in seafood is organic mercury. For example, more than 95% of mercury in fish is methylmercury. It is easy to accumulate through the food chain and cause toxic damage. Moreover, the toxicity of methylmercury is higher than that of free mercury, and organic mercury is more fat-soluble, more easily absorbed by the human gastrointestinal tract, and passes through the blood-brain barrier to act on the central nervous system. Nervous system damage caused by methylmercury poi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N21/78G01N1/28G01N1/34G01N1/44
CPCG01N1/286G01N1/34G01N1/44G01N21/31G01N21/78
Inventor 杨玮娟陈清爱雷丽琴傅依灵
Owner FUJIAN AGRI & FORESTRY UNIV
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