Application of black phosphorus in matrix-assisted laser desorption ionization time-of-flight mass spectrometry
A matrix-assisted laser and time-of-flight mass spectrometry technology, applied in the field of mass spectrometry detection, can solve the problems of inaccurate quantitative analysis and poor sensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0039] [Example 1] Black phosphorus is used as a matrix to detect small molecule compounds
[0040] The black phosphorus used in the present invention was purchased from Xianfeng Nano Material Technology Co., Ltd. (Nanjing, China). Apply black phosphorus directly to the MALDI target to obtain a coating with a diameter of approximately 2 mm. As a comparison, 2 mg / mL CNTs, GO and C60 matrix dispersions were prepared respectively, and 0.5 μL of the dispersions were spotted on the plate as the matrix.
[0041] The analytes are more than 30 kinds of substances with a molecular weight range of 1-1000Da, including acidic substances (acid dyes, acidic surfactants and fatty acids), basic substances (basic dyes and cationic surfactants), amphoteric substances (amino acids and Polypeptides) and polycyclic aromatic hydrocarbons. Take 0.5 μL of the analyte solution and spot it on the matrix layer, and perform MALDI-TOF MS analysis after the solvent evaporates to dryness.
[0042] All mass...
Embodiment 2
[0044] [Example 2] Detection of 4-APC-labeled aldehydes by black phosphorus
[0045] In previous work, we developed the derivatization reagent 4-APC containing quaternary ammonium groups [1] , can be used to derive aldehydes, so in this invention we use black phosphorus to detect 4-APC and 4-APC-labeled five aldehydes: butyraldehyde, hexanal, heptanal, octanal and nonanal.
[0046] The steps for labeling aldehydes with 4-APC are as follows: Add 10 μL of NaBH to 10 μL of aldehyde mixture 3 CN methanol solution (1 mg / mL) and 20 μL of 4-APC methanol solution (1 mg / mL) were dilute to 100 μL with 50 mM ammonium acetate solution (pH 5.7). React for 1h in a constant temperature shaker at 25°C and 1500rpm, and take 0.5μL of the reaction product for MALDI-TOF MS analysis.
[0047] Test results such as image 3 Shown: the detection limit of black phosphorus to 4-APC is 51nM ( image 3 a), at the same time, it has a good detection effect on aldehydes labeled with 4-APC ( image 3 d)...
Embodiment 3
[0048] [Example 3] Black phosphorus detects BQB-labeled sulfhydryl compounds
[0049] In previous work, we developed the derivatization reagent BQB containing quaternary ammonium groups [2] , can be used to derivatize the sulfhydryl groups of thiol compounds, because in the present invention we use black phosphorus to detect four sulfhydryl compounds marked by BQB and BQB: N-acetylcysteine (Nac), glutathione (GSH), Cysteine (Cys) and Homocysteine (HCys).
[0050] The steps for BQB labeling of thiol compounds are as follows: add 10 μL of BQB methanol solution (5 mM) to 10 μL of thiol compound standard mixture, and dilute to 100 μL with glycine hydrochloride buffer solution (5.0 mM, pH 3.5). React for 1h in a constant temperature shaker at 60°C and 1500rpm, and take 0.5μL of the reaction product for MALDI-TOF MS analysis.
[0051] Test results such as image 3 Shown: the detection limit of black phosphorus to BQB is 37nM ( image 3 b), at the same time, it has a good d...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com