The present invention provides a peptide capable of specifically binding to human IgG. In particular, the present invention relates to a human IgG binding peptide tag of 11 to 16 amino acids in length, comprising at least an amino acid sequence of the formula I:
C-(X)n-W-X-X-X-W-(X)m-C
(I)
(SEQ ID NO: 17)
wherein n and m are each an integer of 1 or more and the sum n+m is 4 or 5, wherein X-X-X in the formula I contains no cysteine residue, and
wherein said amino acid sequence satisfies either or both of a) and b):
- a) (X)n—W in the formula I denotes Za-G-Y—W (SEQ ID NO: 18); and
- b) W—(X)m in the formula I denotes W-G-L-Zb (SEQ ID NO: 19)
wherein Za and Zb are each 0, 1, or more amino acid residues.