15 results about "Immunoglobulin genes" patented technology

A continuous directed evolution platform in human B cell lines (CODE-HB) that recruits and repurposes the inherent B cell somatic hypermutation (SHM) mechanisms to rapidly evolve proteins is described. This approach uses stable, non-immunoglobulin loci within the genomes of human B cell lines for integration of a heterologous gene, such as a gene encoding an antibody or antibody fragment. A B-cell surface display platform for displaying fragment antigen binding domains (Fab) of antibodies is also described, which adapts the CODE-HB platform to rapidly evolve antibody fragments in a continuous manner. Plasmids and kits for carrying out these methods are also described.

The application discloses application of Gastrodia elata extract in preparation of a feed additive for resisting Aeromonas hydrophila infection, and belongs to the technical field of water animal disease prevention and treatment. The application first finds that the Gastrodia elata extract has a significant effect of inhibiting Aeromonas hydrophila infection. After the Gastrodia elata extract is added into water animal feed as a feed additive and is fed to the water animal, the Gastrodia elata extract is absorbed into blood circulation system through a digestive tract, up-regulates intestinal immunoglobulin gene expression, improves serum immunoglobulin level, enhances intestinal barrier function, improves animal activity, effectively resists Aeromonas hydrophila infection, reduces disease occurrence, and improves yield and quality of animal breeding. Therefore, the Gastrodia elata extract has a good application prospect.

Non-human animals (and / or non-human cells) and methods of using and making the same are provided, which non-human animals (and / or non-human cells) have a genome comprising human antibody-encoding sequences (i.e., immunoglobulin genes). Non-human animals described herein express antibodies that contain human Igλ light chains, in whole or in part. In particular, non-human animals provided herein are, in some embodiments, characterized by expression of antibodies that contain human Igλ light chains, in whole or in part, that are encoded by human Ig light chain-encoding sequences inserted into an endogenous Igλ light chain locus of said non-human animals. Methods for producing antibodies from non-human animals are also provided.

Non-human animals (and / or non-human cells) and methods of using the same are provided, which non-human animals (and / or non-human cells) have a genome comprising human antibody-encoding sequences (i.e., immunoglobulin genes). Non-human animals described herein express antibodies that are of IgA, IgD, or IgM isotypes. Non-human animals provided herein are, in some embodiments, characterized by expression of IgA antibodies that contain human heavy chain and light chain variable domains and rodent constant domains. Methods for producing antibodies from non-human animals are also provided, which antibodies contain human variable regions and rodent constant regions.

Non-human animals (and / or non-human cells) and methods of using the same are provided, which non-human animals (and / or non-human cells) have a genome comprising human antibody-encoding sequences (i.e., immunoglobulin genes). Non-human animals described herein express antibodies that are of IgA, IgD, or IgM isotypes. Non-human animals provided herein are, in some embodiments, characterized by expression of IgA antibodies that contain human heavy chain and light chain variable domains and rodent constant domains. Methods for producing antibodies from non-human animals are also provided, which antibodies contain human variable regions and rodent constant regions.

The invention relates to the technical field of gene engineering, and particularly discloses a one-step RT-PCR (Reverse Transcription-Polymerase Chain Reaction) kit for amplifying a complete sequence of a variable region of a rabbit-derived antibody and a use method of the one-step RT-PCR kit. The kit comprises an One-Step RT Enzyme Mix, a 5 * One-Step RT Buffer, three pairs of specific amplification primers, a positive control, a negative control and a Nuclease-free Water, and is characterized in that the specific amplification primers are used for amplification of the Nuclease-free Water, and the specific amplification primers are used for amplification of the Nuclease-free Water. The three pairs of primers are designed according to rabbit immune globulin gene sequences and can fully cover heavy chain and light chain variable regions. The use method comprises the following steps: sorting positive B cells from rabbit spleen, cracking single cells, continuously performing reverse transcription and PCR (Polymerase Chain Reaction) amplification in the same reaction system by taking a cracking product as a template, and identifying and cloning the product. The kit can efficiently and specifically obtain the complete sequence of the variable region of the rabbit antibody from the single B cell, and has the advantages of wide coverage, high sensitivity, simplicity and convenience in operation and reliable result.

Disclosed in the present disclosure is genetically modified mice in which immunoglobulin loci are modified to insert gene segments of a human immunoglobulin variable region. The mice can be bred normally and produce human-mouse chimeric antibodies including a human variable region and a mouse constant region. The present disclosure also provides a method for preparing the genetically modified mice and use of the mice.

Provided in the present invention are a genetically modified animal having a restricted humanized light chain immunoglobulin locus, a method for producing the animal, and a method for producing a common light chain antibody and an antibody library thereof using the animal. The restricted humanized light chain immunoglobulin locus comprises a portion of a V region and a portion of a J region from a light chain Kappa variable region locus of a human immunoglobulin.

Disclosed in the present disclosure is genetically modified mice in which immunoglobulin loci are modified to insert gene segments of a human immunoglobulin variable region. The mice can be bred normally and produce human-mouse chimeric antibodies including a human variable region and a mouse constant region. The present disclosure also provides a method for preparing the genetically modified mice and use of the mice.

The present application relates to a genetically modified non-human animal comprising a humanized heavy chain immunoglobulin locus and / or a humanized light chain immunoglobulin locus and methods of making the same. The present application also provides methods of producing antibodies by the non-human animal.

The present disclosure relates to genetically modified animals and cells having a humanized lambda light chain immunoglobulin locus and / or a humanized heavy chain immunoglobulin locus. The disclosure also relates to genetically modified animals and cells having a modified locus comprising a human lambda light chain immunoglobulin gene and / or a humanized heavy chain immunoglobulin locus. In some embodiments, the animal also has a humanized kappa light chain immunoglobulin locus.

The present disclosure relates to genetically modified animals and cells having a humanized lambda light chain immunoglobulin locus and / or a humanized heavy chain immunoglobulin locus. The disclosure also relates to genetically modified animals and cells having a modified locus comprising a human lambda light chain immunoglobulin gene and / or a humanized heavy chain immunoglobulin locus. In some embodiments, the animal also has a humanized kappa light chain immunoglobulin locus.

Genetically modified non-human animals are provided that express an immunoglobulin variable domain that comprises at least one histidine, wherein the at least one histidine is encoded by a substitution of a non-histidine codon in the germline of the animal with a histidine codon, or the insertion of a histidine codon in a germline immunoglobulin nucleic acid sequence. Immunoglobulin genes comprising histidines in one or more CDRs, in an N-terminal region, and / or in a loop 4 region are also provided. Immunoglobulin variable domains comprising one or more histidines (e.g., histidine clusters) substituted for non-antigen-binding non-histidine residues. Non-human animals that are progeny of animals comprising modified heavy chain variable loci (V, D, J segments), modified light chain variable loci (V, J segments), and rearranged germline light chain genes (VJ sequences) are also provided. Non-human animals that make immunoglobulin domains that bind antigens in a pH-sensitive manner are provided.

Non-human animals (and / or non-human cells) and methods for using said non-human animals (and / or non-human cells) are provided, said non-human animals (and / or non-human cells) having a genome containing sequences encoding human antibodies (i.e., immunoglobulin genes). The non-human animals described herein express antibodies containing immunoglobulin (Ig) light chains, said immunoglobulin light chains being characterized by the presence of a human Vλ domain. In some embodiments, the non-human animals provided herein are characterized by expressing antibodies containing a human Vλ light chain encoded by a sequence encoding a human Igλ light chain inserted into an endogenous Igκ light chain locus of said non-human animal. Methods for producing antibodies from non-human animals are also provided, said antibodies containing a human variable region and a mouse constant region.