3 results about "Nuclear reprogramming" patented technology

This document provides materials and methods involved in making and using induced pluripotent stem cells (iPSCs). For example, measles virus vectors for reprogramming somatic cells into iPSC's, methods for obtaining iPSCs, and methods for using iPSCs are provided.

The invention belongs to the technical field of cytology, and discloses application of a chromatin remodeling factor Smarcb1 in preparation of a protein marker for regulating and controlling in-vitro cloned embryo epigenetic modification of animal cells. And the chromatin remodeling factor Smarcb1 comprises a variable spliceosome Smarcb1.1 of the chromatin remodeling factor Smarcb1. The invention relates to an oocyte transplanted by using a Smarcb1.1 expression vector constructed by the Smarcb1.1. According to the invention, it is determined that the Smarcb1 adjusts the development condition of the cloned embryo through cell nucleus reprogramming, and the action mechanism of the Smarcb1 on SCNT embryo development is disclosed, so that the developmental rate of the cloned embryo is improved, and the development and application of a somatic cell nucleus transfer technology are promoted.

The present invention provides methods and compostions to improve the efficiency of somatic cell nuclear transfer (SCNT) and the consequent production of nuclear transfer ESC (ntESC) and transgenic cells and / or non-human animals. More specifically, the present invention relates to the discovery that trimethylation of Histone H3-Lysine 9 (H3K9me3) in reprogramming resistant regions (RRRs) in the nuclear genetic material of donor somatic cells prevents efficient somatic cell nuclear reprogramming or SCNT. The present invention provide methods and compositions to decrease H3K9me3 in methods to improve efficacy of SCNT by exogenous or overexpression of the demethylase Kdm4 family and / or inhibiting methylation of H3K9me3 by inhibiting the histone methyltransferases Suv39h1 and / or Suv39h2.