124 results about "Transcription Activation" patented technology

The invention discloses a SbWRKY51 gene, a promoter and application of the SbWRKY51 gene and the promoter in improvement of salt tolerance of sorghum, and belongs to the technical field of plant genetic engineering. The SbWRKY51 gene is screened from sorghum, and the expression level of the SbWRKY51 gene is up-regulated after salt stress treatment. The protein coded by the gene belongs to a WRKY transcription factor family, has transcriptional activation activity, and can start the expression of a reporter gene. Sorghum overexpression strains and complementary strains of the gene are obtained, salt tolerance related physiological indexes are measured, overexpression of the gene can improve the seed germination rate, increase the seed root length, regulate ion balance and enhance the free radical scavenging capacity of plants, and the effect of the gene in the aspect of improving the salt tolerance is embodied. A further research finds that the SbWRKY51 gene can enhance the plant salt tolerance by regulating a lignin synthesis pathway. The technical scheme provides a basis for cultivating resistant plants, and has important guiding significance for enhancing the production potential of sorghum under a high-salt condition and promoting agricultural development.

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA3 gene, an expression vector and application of the CeNF-YA3 gene in vegetable fat regulation and control. The nucleotide sequence of the CeNF-YA3 gene is as shown in SEQ ID NO.1. After the CeNF-YA3 gene is over-expressed, the grease content of arabidopsis seeds and leaves can be remarkably increased; in addition, the invention also provides a series of vectors containing the CeNF-YA3 gene for subcellular localization, yeast hybridization and plant overexpression for the first time, and proves that the CeNF-YA3 protein has a transcriptional activation function, is localized in a cell nucleus and accords with the basic characteristics of transcription factors for the first time. Therefore, the technical scheme of the invention provides scientific guidance for the application of the CeNF-YA3 gene and the protein thereof.

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA1 gene, an expression vector and application of the CeNF-YA1 gene in vegetable fat regulation and control. The invention provides the CeNF-YA1 gene for the first time, the nucleotide sequence of the CeNF-YA1 gene is as shown in SEQ ID NO.1, the full length of a coding region of the CeNF-YA1 gene is 792 bp, a series of carriers containing the CeNF-YA1 gene, such as subcellular localization, yeast hybridization and plant overexpression, are constructed for the first time, and meanwhile, the CeNF-YA1 protein is proved to have a transcription activation function, is localized in a cell nucleus and conforms to the basic characteristics of transcription factors. It is found for the first time that overexpression of the CeNF-YA1 gene can significantly improve the grease content of arabidopsis seeds and leaves; therefore, the technical scheme provided by the invention can be used for improving the oil content of the plant seeds and the nutritional tissues, and shows the application prospect in improving the oil content of the seeds and the nutritional tissues.

The invention provides a kiwi fruit transcription factor and application thereof in kiwi fruit canker, and belongs to the technical field of genetic engineering. The invention provides a transcription factor AcWRKY50, the nucleotide sequence of the transcription factor AcWRKY50 is shown as SEQ ID No.1, and the AcWRKY50 is positioned in a cell nucleus and has transcription activation capability. The AcWRKY50 disclosed by the invention can continuously respond to Psa infection, after Psa pathogenic bacteria infection, the expression of the AcWRKY50 gene is rapidly activated and continuously up-regulated, and after the AcWRKY50 gene is over-expressed, the resistance to Psa can be improved. Meanwhile, MeJA negatively regulates the resistance of the kiwi fruit to the Psa, and the kiwi fruit germplasm can be innovated based on the transcription factor disclosed by the invention.

The invention provides an artificial plant bidirectional constitutive strong promoter, and particularly relates to a high-activity short transcription enhancer fragment which is formed by connecting three plant endogenous fragments and a plant virus in series and serves as a transcription activation basic element. A minimum core promoter element Mini 35S from a cauliflower mosaic virus and a minimum core promoter element CsVMV mini-pro functional module from a cassava vein mosaic virus are respectively integrated at two ends, so that the plant constitutive strong promoter with bidirectional starting capability is constructed. According to the invention, a synthetic quadruple STE transcription enhancer, a Mini 35S promoter and a CsVMV mini-pro promoter are functionally coupled to form an expression regulation tool with broad-spectrum adaptability to plant species, and two operably connected target gene expression cassettes can be simultaneously driven by a short regulation sequence to realize synergistic efficient expression in plant cells.

The present invention relates to a method, in particular an in vitro method, for identifying an improved anti-senescence compound based on detecting the binding of said compound in the presence of at least one phosphory lated amino acid in the transcription activation domain (TAD) domain of mammalian protein p53. The present invention further relates to a method, in particular an in vitro method, for monitoring an anti-senescence treatment or prophylaxis in a mammalian subject in need thereof, based on detecting the amount of phosphorylation of amino acids in the TAD and / or C-terminal region of the mammalian p53 protein in a biological sample obtained from said subject and / or detecting the amount and / or co-localization with phosphorylated p53 of the promyelocytic leukemia protein (PML) bodies in a biological sample obtained from said subject. Furthermore, the present invention relates to a kit for performing the above methods as well as respective uses thereof. Finally, improved anti-senescence compounds or pharmaceutical compositions are provided.

The invention discloses a CRISPRa tool for activating a target gene and application of the CRISPRa tool. The invention provides a protein. The protein sequentially comprises the following elements from an N terminal to a C terminal: a dCas9 segment and an activation domain segment, n NHRs are arranged in the active domain section; n is a natural number; the NHR is an NHR activating element from a nematode. The NHR has a nonaamino acid trans-activation domain (9aa TAD), consists of only 16 amino acid residues, and shows high transcriptional activity. The invention provides a CRISPRa tool with stronger action effect and more concise action element, can be used for gene transcription regulation (particularly gene transcription activation), can be used in the scientific research field and the clinical medicine field, and has great value.

The invention relates to the technical field of biological medicine, in particular to a nuclear receptor truncated variant and application thereof. According to the truncated variant of the nuclear receptor LBD structural domain provided by the invention, at least one amino acid is deleted at the C terminal. The AR truncated variant can induce transcriptional activation of a downstream signal channel under the condition of deletion of AR-NTD, and a brand new mechanism analysis idea and a treatment target are provided for solving the problem that AR-negative prostate cancer patients are difficult to treat.

The invention relates to the technical field of biological medicine, and relates to a polypeptide in targeted combination with SOX2 protein and application thereof, the polypeptide has an amino acid sequence as shown in SEQ ID NO.1, or has at least 85%, 90%, 95% or more than 99% of sequence identity with the amino acid sequence; the polypeptide inhibits phase separation of SOX2, liquid drops formed by phase separation of SOX2 can be avoided, then the situation that the liquid drops wrap chemotherapeutic drugs, influence the curative effect of the chemotherapeutic drugs and cause tolerance of tumor cells to the chemotherapeutic drugs is avoided, and the polypeptide does not influence the transcription activation function of SOX2 serving as a transcription factor; the polypeptide can inhibit the phase separation of the SOX2, so that the regulation of normal physiological functions, such as high expression in organs such as eyes of a human body, of the SOX2 as a transcription factor is not influenced, and therefore, the polypeptide disclosed by the invention can reverse chemotherapy drug resistance of tumors such as squamous carcinoma by inhibiting the phase separation of the SOX2, can be used for adjuvant therapy of the tumors such as squamous carcinoma, and has no obvious toxic or side effect on each organ of an organism.

This invention belongs to the field of biotechnology and relates to a high-concentration carbon dioxide-tolerant *Synechococcus* species, its preparation method, and its applications. The high-concentration carbon dioxide-tolerant *Synechococcus* species includes a CRISPR / dCas9 plasmid, a gRNA plasmid, and a knockout ω plasmid. This invention enables the growth of PCC7942 under high CO2 conditions by transcribedly activating PEPC using the CRISPR / dCas9 system. Furthermore, the fusion between the ω subunit of RNAP and a Cas9 nuclease mutant that binds to the upstream promoter region allows for programmable transcriptional activation. By knocking out the ω subunit, the CRISPR / dCas9 transcriptional regulation system is used to optimize the microalgal photosynthetic carbon fixation pathway, thereby improving the growth and carbon fixation efficiency of microalgae under high CO2 conditions.

The invention provides an application of Exifenone in preparation of a medicine for treating diabetic retinopathy, the Exifenone can be combined near a K412 lactic acid modification site of HDAC1, so that the lactic acid modification of the K412 site of the HDAC1 is specifically inhibited, the deacetylation activity of the HDAC1 to H3K56 is enhanced, the transcriptional activation of VEGFA (vascular endothelial growth factor A) mediated by H3K56ac and pathological angiogenesis are inhibited, and the effect of treating diabetic retinopathy is achieved. Further, vascular pathological injury caused by diabetic retinopathy is improved. The application provides a new target and a candidate drug for clinical treatment of diabetic retinopathy. The research result of the application provides experimental evidence for the epoxybenzophenone to be used for clinical specific treatment of diabetic retinopathy.

The invention belongs to the technical field of kit detection, discloses a chromatin modification detection kit for regulating ATIC expression through PRMT8, and discovers the specific expression and cancer promotion function of arginine methyltransferase PRMT8 and purine de novo synthesis key enzyme ATIC in triple negative breast cancer for the first time. The specific biological effect of the PRMT8 in the triple negative breast cancer is researched for the first time, and the PRMT8 promotes ATIC transcriptional activation by mediating phosphorylation of an ATIC promoter region H4R3me2a and an effect protein TDRD3 of the PRMT8. The important function of arginine methyltransferase PRMT8 in a tumor purine metabolic pathway is found by gradually exploring the transcriptional activation process of purine metabolic rate-limiting enzyme ATIC, the post-translational modification function of histone is associated with the activation of the breast cancer purine metabolic pathway, and a new theoretical basis is provided for treating breast cancer by targeting PRMT8.

The present application relates to a kind of FOXM1 protein phosphorylation modification interfering peptide and its application, belong to the field of biological medicine technology. Specifically, the present application is found in research, the transcriptional activity of FOXM1 protein depends on the condensate formed by its polymerization, destroying the polymerization of FOXM1 can effectively inhibit the occurrence and development of tumor, and the phosphorylation of the 376th amino acid of FOXM1 protein can also inhibit polymerization to some extent, thereby inhibiting the transcriptional activation of FOXM1 protein. Therefore, the present application designs an interfering peptide, and introduces phosphorylation modification in appropriate position, obtains a kind of phosphorylation modification interfering peptide FIP4 targeted to FOXM1 protein polymerization domain, the interfering peptide plays a significant role in inhibiting the transcriptional activation of FOXM1 protein in cell and animal in vivo, and can be used for treating tumor-related diseases.

The invention discloses a SbASR4 gene for regulating and controlling plant salt stress tolerance as well as an encoding protein and application thereof. The SbASR4 gene is derived from Sorghum baicola, and the encoded protein is simultaneously positioned in a cell membrane and a cell nucleus, does not have transcriptional activation activity, and is a negative regulation factor of plant salt stress response. The overexpression of the gene can significantly increase the sensitivity of the plant to salt and ABA, and the silence or knockout of the gene can greatly improve the salt tolerance of the plant. Mechanism research shows that the SbASR4 protein directly interacts with an SOS signal channel core kinase SOS2 in a plant body, and the SOS channel is negatively regulated through the interaction, so that the expression of a downstream ion transport gene is inhibited. The invention provides a method for improving the salt tolerance of plants by reducing the expression of SbASR4 or destroying the interaction of SbASR4 and SOS2, related genetic materials and products, and a brand new target and an effective strategy are provided for salt-resistant breeding of crops.

The invention relates to the technical field of biological medicine, in particular to a truncated variant of a nuclear receptor LBD region and application of the truncated variant. According to the truncated variant of the nuclear receptor LBD structural domain provided by the invention, at least one amino acid is deleted at the C terminal. The ER, PR and GR truncated variants can induce transcriptional activation of a downstream signal channel under the condition that NTD is deleted, and a brand new treatment target is provided for patients with cancers such as prostatic cancer, breast cancer, endometrial cancer, acute lymphocytic leukemia, lymphoma, pancreatic cancer and lung cancer.

Provided herein are plants, plant parts, a population of plants or plant parts, and plant products (e.g., seed composition, protein composition) comprising reduced activity of a protein-related polypeptide [e.g., stomatal cytokinesis defective 2 (SCD2), SCD2A, SCD2B, response to dehydration 22 (RD22), glucuronidase 3 (GUS3), GUS3A, glycosyl hydrolase family 10 protein B (GH10B), protein phosphatase 2A beta subunit (PP2AB), PPA2BA, PP2ABAB, alpha / beta-hydrolases superfamily protein (ABH), ABHA, ABHB, calmodulin-binding transcription activator protein 2 (CAMTA2), CAMTA2A, CAMTA2B, cinnamyl-alcohol dehydrogenase (CAD1), beta-ketoacyl reductase 1 (KCR1), KCR1A, or KCR1B], and compositions and methods of producing such plants and plant parts. The plants, plant parts, population of plants or plant parts, or plant products can have a genetic mutation that reduces the protein-related polypeptide activity, which can be one located at least partially in a protein-related gene (e.g., CAD1) or its homolog or in its regulatory region, and can have increased protein content and / or white flake protein content.

A clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for adaptive antiviral defence (Cascade); the Cascade protein complex comprising at least CRISPR-associated protein subunits Cas7, Cas5 and Cas6 which includes at least one subunit with an additional amino acid sequence possessing nucleic acid or chromatin modifying, visualising, transcription activating or transcription repressing activity. The Cascade complex with additional activity is combined with an RNA molecule to produce a ribonucleoprotein complex. The RNA molecule is selected to have substantial complementarity to a target sequence. Targeted ribonucleoproteins can be used as genetic engineering tools for precise cutting of nucleic acids in homologous recombination, non-homologous end joining, gene modification, gene integration, mutation repair or for their visualisation, transcriptional activation or repression. A pair of ribonucleotides fused to FokI dimers may be used to generate double-strand breakages in the DNA to facilitate these applications in a sequence-specific manner.

The invention relates to a synthesis method of alkaloid, and discloses a synthesis method of alkaloid (-)-psychotriadine and analogues thereof, and the synthesis method comprises the following steps: taking a tryptamine derivative as an initial raw material, taking an asymmetric Meerwein-Eschenmoser-Claisen rearrangement reaction and a Fischer indolation and Plancher rearrangement reaction as key reactions, and realizing the preparation of (-)-psychotriadine through a nine-step linear synthesis route. The method disclosed by the invention has gram-level amplification capability, and the problem of medicine source bottleneck of a natural sample and homologues thereof in future medicine research can be solved; meanwhile, it is verified through a Luciferase reporter gene experiment that (-)-psychotriadine and tetrahydropsychotriadine have the transcriptional activation activity of LXR [alpha] and LXR [beta], and the (-)-psychotriadine and the tetrahydropsychotriadine show the potential treatment effect on the Alzheimer's disease.

The application belongs to the field of molecular biological technology, and particularly relates to CeABI3B a gene, an expression vector and application thereof in plant oil regulation. CeABI3B The application provides a gene for the first time, a nucleotide sequence of which is shown as SEQ ID NO. 1, and a coding region of which has a full length of 1707 bp. CeABI3B A series of vectors containing the gene are constructed for the first time, including subcellular localization, yeast hybridization and plant overexpression, and it is proved that the CeABI3B protein has a transcription activation function and is located in the cell nucleus, which is in line with the basic characteristics of transcription factors. CeABI3B Not only is it found that transient overexpression of the gene can significantly improve the oil content of tobacco leaves, but also it is proved that overexpression of the gene can significantly improve the oil content of transgenic Arabidopsis thaliana seeds and leaves. CeABI3B Therefore, the cloned and identified gene can be used to improve the oil content of plant seeds and nutrient tissues, and has a wide application prospect.

The invention relates to the technical field of plant molecular biology, in particular to application of OsMYB52 protein or a coding gene of the OsMYB52 protein or a biological material containing the coding gene of the OsMYB52 protein to regulation and control of the heading stage of plants. The invention discloses an MYB transcription factor OsMYB52 for negative regulation and control of the heading stage of rice. The gene interacts with Hd1 to enhance the transcriptional activation effect of Hd1 on Ghd7 and Ghd7.1 and inhibit the transcriptional activation of Hd1 on Ehd1 and Hd3a, so that the heading stage of rice is negatively regulated, and the rice yield is changed. The function and the regulation mechanism of the OsMYB52 for regulating the heading stage of the rice are reported for the first time, and the OsMYB52 has important guiding significance for perfecting the molecular mechanism of a rice flowering regulation network and improving the yield and the quality of the rice.

The present application relates to the technical field of plant molecular biology, and particularly relates to application of OsMYB52 protein, or a coding gene thereof, or a biological material containing the coding gene of the OsMYB52 protein in regulating a heading stage of a plant. The present application discloses a MYB transcription factor for negatively regulating a heading stage of rice OsMYB52 . The gene enhances transcription activation of Hd1 on Ghd7 , Ghd7.1 by interacting with Hd1, inhibits transcription activation of Hd1 on Ehd1 , Hd3a , and further negatively regulates the heading stage of rice and causes change of yield of rice. OsMYB52 The function and regulation mechanism of regulating the heading stage of rice are reported for the first time, and the function and regulation mechanism have important guiding significance for perfecting a molecular mechanism of a flowering regulation network of rice and improving yield and quality of rice.

The present invention relates to a cell-free transcriptional electrochemical biosensor and to the use of the same for detecting specific molecular analytes, such as specific antibodies, proteins, small molecules, nucleic acids, and derivatives thereof, in complex arrays of biological samples, such as plasma, serum, blood, saliva, sweat, and the like, wherein said biosensor is based on the activation of the transcription of a specific RNA strand, induced by recognition with the analyte. The invention further relates to a method for the detection of specific molecular analytes in complex arrays of biological samples, said method being based on the use of said cell-free transcriptional electrochemical biosensor.

The present invention discloses a switchgrass transcription factor gene PvC3H12 for improving plant cold tolerance and its application, belonging to the technical field of genetic engineering. The CDS sequence of the switchgrass transcription factor gene PvC3H12 is as shown in SEQ ID NO.7. This gene is localized in the nucleus and exhibits obvious transcriptional activation activity in yeast, indicating that PvC3H12 is a transcriptional activator. Further research found that overexpression of PvC3H12 significantly improved the cold tolerance of switchgrass. The acquisition of the switchgrass transcription factor gene PvC3H12 of the present invention is expected to improve the cold tolerance of multiple perennial warm-season plants and provide a theoretical basis for cultivating plants with high cold tolerance.

This invention belongs to the field of biomedicine and regenerative medicine technology. It discloses a chimeric polypeptide, related biomaterials, and their application in inducing cardiomyocytes. The chimeric polypeptide is obtained through family homologous domain substitution and is selected from the following groups: (1) a MEF2 chimeric polypeptide with MEF2C protein as the backbone, wherein the N-terminal and C-terminal transcriptional activation domains are replaced by homologous regions of MEF2B and MEF2D, respectively; (2) a GATA chimeric polypeptide with GATA4 protein as the backbone, wherein the N-terminal transcriptional activation domain is replaced by homologous regions of GATA1 and GATA6. This invention also provides the nucleic acid encoding the polypeptide, the construct, and a method for efficiently inducing fibroblast reprogramming into cardiomyocytes under in vitro conditions. When the chimeric polypeptide of this invention is used alone or in combination, the induced cells exhibit calcium ion oscillations earlier and have higher maturity.

The application discloses a transcription factor SrMYB1 for regulating synthesis of steviol glycoside, an expression protein thereof and application, and belongs to the technical field of plant genetic engineering. The transcription factor SrMYB1 has a nucleotide sequence as shown in SEQ ID NO. 1, and the amino acid sequence of the expression protein is as shown in SEQ ID NO. 2. Subcellular localization in tobacco epidermal cells shows that the protein coded by the gene is mainly located in the nucleus; yeast activity experiment shows that the protein has transcription activation activity. Yeast single hybridization experiment shows that SrMYB1 can be combined on the promoter of a key gene for synthesis of steviol glycoside SrUGT76G1 . Promoter activity and transgenic experiment both show that SrMYB1 significantly inhibits transcription of the key gene for synthesis of steviol glycoside SrUGT76G1 . Based on the characteristics, certain reference can be provided for quality regulation of steviol glycoside and cultivation of new steviol varieties.

A transcription activating protein TraR of a TraR / 3-oxo-C8-HSL expression-inducing system. The transcription activating protein TraR has a transcription activating domain, and the transcription activating domain contains an NF-κB p65 sequence and a heat shock factor 1 sequence. Further provided are a nucleic acid encoding the transcription activating protein TraR, a cell and an expression-inducing system containing the transcription activating protein TraR, and a method for using the expression-inducing system to regulate the expression of a target nucleic acid.

The invention discloses a CRISPR (clustered regularly interspaced short palindromic repeats) gene activation system and a method for increasing theanine content in tea trees by the aid of the CRISPR gene activation system, and belongs to the field of plant gene engineering. The method for increasing the content of theanine in the tea tree comprises the following steps: constructing a CRISPR (clustered regularly interspaced short palindromic repeats) gene activation system which comprises an expression element of dCas9-transcriptional activator fusion protein and at least one sgRNA expression element of a target theanine biosynthesis pathway key gene promoter region; the system is introduced into tea tree cells or tissues, so that transcription of endogenous target genes is specifically activated, and the theanine synthesis amount is increased. Compared with the prior art, the CRISPR-Act platform is established in the tea tree for the first time, synchronous, stable and efficient activation of theanine synthesis pathway polygenes is achieved by using the endogenous efficient promoter, and a powerful and accurate technical means is provided for cultivation of new varieties of high-theanine tea trees and biological manufacturing of theanine.