187 results about "Transcription Activation" patented technology

The invention discloses a SbWRKY51 gene, a promoter and application of the SbWRKY51 gene and the promoter in improvement of salt tolerance of sorghum, and belongs to the technical field of plant genetic engineering. The SbWRKY51 gene is screened from sorghum, and the expression level of the SbWRKY51 gene is up-regulated after salt stress treatment. The protein coded by the gene belongs to a WRKY transcription factor family, has transcriptional activation activity, and can start the expression of a reporter gene. Sorghum overexpression strains and complementary strains of the gene are obtained, salt tolerance related physiological indexes are measured, overexpression of the gene can improve the seed germination rate, increase the seed root length, regulate ion balance and enhance the free radical scavenging capacity of plants, and the effect of the gene in the aspect of improving the salt tolerance is embodied. A further research finds that the SbWRKY51 gene can enhance the plant salt tolerance by regulating a lignin synthesis pathway. The technical scheme provides a basis for cultivating resistant plants, and has important guiding significance for enhancing the production potential of sorghum under a high-salt condition and promoting agricultural development.

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA3 gene, an expression vector and application of the CeNF-YA3 gene in vegetable fat regulation and control. The nucleotide sequence of the CeNF-YA3 gene is as shown in SEQ ID NO.1. After the CeNF-YA3 gene is over-expressed, the grease content of arabidopsis seeds and leaves can be remarkably increased; in addition, the invention also provides a series of vectors containing the CeNF-YA3 gene for subcellular localization, yeast hybridization and plant overexpression for the first time, and proves that the CeNF-YA3 protein has a transcriptional activation function, is localized in a cell nucleus and accords with the basic characteristics of transcription factors for the first time. Therefore, the technical scheme of the invention provides scientific guidance for the application of the CeNF-YA3 gene and the protein thereof.

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA1 gene, an expression vector and application of the CeNF-YA1 gene in vegetable fat regulation and control. The invention provides the CeNF-YA1 gene for the first time, the nucleotide sequence of the CeNF-YA1 gene is as shown in SEQ ID NO.1, the full length of a coding region of the CeNF-YA1 gene is 792 bp, a series of carriers containing the CeNF-YA1 gene, such as subcellular localization, yeast hybridization and plant overexpression, are constructed for the first time, and meanwhile, the CeNF-YA1 protein is proved to have a transcription activation function, is localized in a cell nucleus and conforms to the basic characteristics of transcription factors. It is found for the first time that overexpression of the CeNF-YA1 gene can significantly improve the grease content of arabidopsis seeds and leaves; therefore, the technical scheme provided by the invention can be used for improving the oil content of the plant seeds and the nutritional tissues, and shows the application prospect in improving the oil content of the seeds and the nutritional tissues.

Disclosed herein are compositions of transcription activator-like effectors transcription factors and methods of using the compositions for inducing gene expression of mammalian genes.

The present invention relates to a cell comprising a chimeric antigen receptor (CAR) and a constitutively active or inducible Signal Transducer and Activator of Transcription (STAT) molecule.

The invention relates to construction and application of a plant cell expression tool, and particularly provides a nucleic acid construct which comprises a chimeric promoter and a coding sequence of a fusion protein, wherein the chimeric promoter comprises a protein binding region and a core promoter sequence, the fusion protein comprises a nucleic acid binding structural domain and a transcriptional activation structural domain, and the expression tool can improve expression of target protein in plant cells.

The invention discloses an application of a tomato B3-337 transcription factor in improvement of tomato quality, a gene for coding a B3-337 transcription factor protein is named as a B3-337 gene, and the nucleotide sequence is shown as SEQ ID NO.1. The invention also discloses an application of the tomato B3-337 transcription factor in improvement of tomato quality. According to the application, a carrier pFM3100GFP-B3-337 is constructed, so that the B3-337 is proved to be positioned in a cell nucleus. A transcriptional activity detection result proves that B3-337 has transcriptional activation activity. RT-qPCR (real-time quantitative polymerase chain reaction) and GUS (glucuronidase) dyeing show that the transcription factor is expressed in pericarp, seed, placenta and center pillar of each fruit ripening stage. According to the invention, a B3-337 gene overexpression plant is obtained by constructing a B3-337 overexpression vector, and compared with a wild type, the tomato fruit overexpressed with the B3-337 gene has the advantages that the content of soluble solids brix is increased, the fruit becomes larger, and the weight becomes heavier. It is shown that the B3-337 transcription factor plays an important regulation role in positive regulation of tomato fruit quality. The invention lays a good gene genetic resource foundation for the cultivation of new varieties of high-quality tomatoes.

The invention provides a kiwi fruit transcription factor and application thereof in kiwi fruit canker, and belongs to the technical field of genetic engineering. The invention provides a transcription factor AcWRKY50, the nucleotide sequence of the transcription factor AcWRKY50 is shown as SEQ ID No.1, and the AcWRKY50 is positioned in a cell nucleus and has transcription activation capability. The AcWRKY50 disclosed by the invention can continuously respond to Psa infection, after Psa pathogenic bacteria infection, the expression of the AcWRKY50 gene is rapidly activated and continuously up-regulated, and after the AcWRKY50 gene is over-expressed, the resistance to Psa can be improved. Meanwhile, MeJA negatively regulates the resistance of the kiwi fruit to the Psa, and the kiwi fruit germplasm can be innovated based on the transcription factor disclosed by the invention.

The invention provides a method for enhancing transcriptional activation of yeast and application. The strong activation polypeptide is a yeast source activation polypeptide mutant, a human source activation polypeptide or a fusion thereof, can realize high-intensity transcriptional activation of a promoter in a yeast cell, and can realize high-efficiency expression of an exogenous recombinant protein when the strong activation polypeptide is adaptively used in a related expression tool box. According to the strong activation polypeptide disclosed by the invention, the recombinant expression intensity of pichia pastoris is remarkably improved.

The disclosure is generally directed to spherical nucleic acids (SNAs), nanostructures with a core surrounded by a radial presentation of oligonucleotides, that can activate a cytoplasmic DNA sensor including but not limited to cyclic GMP-AMP synthase (cGAS). In some embodiments, the SNAs also inactivate a transcription factor including but not limited to signal transducer and activator of transcription 3 (STATS). Methods of making and using the SNAs are also provided herein. In some aspects, the present disclosure provides a spherical nucleic acid (SNA) comprising (a) a nanoparticle core; and (b) a shell of oligonucleotides attached to the external surface of the nanoparticle core, the shell of oligonucleotides comprising a double-stranded or single-stranded stem loop DNA oligonucleotide that activates cyclic GMP-AMP synthase (cGAS) and is at least 15 base pairs in length.

The invention relates to application of a plant transcription factor PHR1 active site. The active sites of the plant transcription factor PHR1 comprise serine at the 291th site in arabidopsis thaliana AtPHR1 and threonine at the 296th site in corn ZmPHR1. Through phosphorylation modification on the active site of the Arabidopsis thaliana transcription factor AtPHR1, the transcriptional activation activity of the transcription factor PHR1 can be enhanced, the protein stability of the AtPHR1 is improved, and the inorganic phosphorus content of plants is increased. According to amino acid sequence comparison, threonine at the 296th site of the corn ZmPHR1 and serine at the 291th site of the arabidopsis thaliana AtPHR1 are conserved in evolution, and phosphorylation modification of the threonine at the 296th site of the corn ZmPHR1 also promotes activation activity of transcription factors of the corn ZmPHR1. The active site of the plant transcription factor PHR1 provided by the invention can provide a direction for cultivating stress-resistant variety plants capable of efficiently absorbing and utilizing phosphorus and promote the development of modern basic biology and agricultural breeding.

The invention provides an artificial plant bidirectional constitutive strong promoter, and particularly relates to a high-activity short transcription enhancer fragment which is formed by connecting three plant endogenous fragments and a plant virus in series and serves as a transcription activation basic element. A minimum core promoter element Mini 35S from a cauliflower mosaic virus and a minimum core promoter element CsVMV mini-pro functional module from a cassava vein mosaic virus are respectively integrated at two ends, so that the plant constitutive strong promoter with bidirectional starting capability is constructed. According to the invention, a synthetic quadruple STE transcription enhancer, a Mini 35S promoter and a CsVMV mini-pro promoter are functionally coupled to form an expression regulation tool with broad-spectrum adaptability to plant species, and two operably connected target gene expression cassettes can be simultaneously driven by a short regulation sequence to realize synergistic efficient expression in plant cells.

The present invention relates to a method, in particular an in vitro method, for identifying an improved anti-senescence compound based on detecting the binding of said compound in the presence of at least one phosphory lated amino acid in the transcription activation domain (TAD) domain of mammalian protein p53. The present invention further relates to a method, in particular an in vitro method, for monitoring an anti-senescence treatment or prophylaxis in a mammalian subject in need thereof, based on detecting the amount of phosphorylation of amino acids in the TAD and / or C-terminal region of the mammalian p53 protein in a biological sample obtained from said subject and / or detecting the amount and / or co-localization with phosphorylated p53 of the promyelocytic leukemia protein (PML) bodies in a biological sample obtained from said subject. Furthermore, the present invention relates to a kit for performing the above methods as well as respective uses thereof. Finally, improved anti-senescence compounds or pharmaceutical compositions are provided.

The invention discloses a CRISPRa tool for activating a target gene and application of the CRISPRa tool. The invention provides a protein. The protein sequentially comprises the following elements from an N terminal to a C terminal: a dCas9 segment and an activation domain segment, n NHRs are arranged in the active domain section; n is a natural number; the NHR is an NHR activating element from a nematode. The NHR has a nonaamino acid trans-activation domain (9aa TAD), consists of only 16 amino acid residues, and shows high transcriptional activity. The invention provides a CRISPRa tool with stronger action effect and more concise action element, can be used for gene transcription regulation (particularly gene transcription activation), can be used in the scientific research field and the clinical medicine field, and has great value.

The invention relates to the technical field of biological medicine, in particular to a nuclear receptor truncated variant and application thereof. According to the truncated variant of the nuclear receptor LBD structural domain provided by the invention, at least one amino acid is deleted at the C terminal. The AR truncated variant can induce transcriptional activation of a downstream signal channel under the condition of deletion of AR-NTD, and a brand new mechanism analysis idea and a treatment target are provided for solving the problem that AR-negative prostate cancer patients are difficult to treat.

The invention relates to the technical field of biological medicine, and relates to a polypeptide in targeted combination with SOX2 protein and application thereof, the polypeptide has an amino acid sequence as shown in SEQ ID NO.1, or has at least 85%, 90%, 95% or more than 99% of sequence identity with the amino acid sequence; the polypeptide inhibits phase separation of SOX2, liquid drops formed by phase separation of SOX2 can be avoided, then the situation that the liquid drops wrap chemotherapeutic drugs, influence the curative effect of the chemotherapeutic drugs and cause tolerance of tumor cells to the chemotherapeutic drugs is avoided, and the polypeptide does not influence the transcription activation function of SOX2 serving as a transcription factor; the polypeptide can inhibit the phase separation of the SOX2, so that the regulation of normal physiological functions, such as high expression in organs such as eyes of a human body, of the SOX2 as a transcription factor is not influenced, and therefore, the polypeptide disclosed by the invention can reverse chemotherapy drug resistance of tumors such as squamous carcinoma by inhibiting the phase separation of the SOX2, can be used for adjuvant therapy of the tumors such as squamous carcinoma, and has no obvious toxic or side effect on each organ of an organism.

Compositions comprising biologically active synthetic nanoparticle constructs and methods of use thereof to modify mitochondrial gene expression including transcriptional repression and transcriptional activation.

This invention belongs to the field of biotechnology and relates to a high-concentration carbon dioxide-tolerant *Synechococcus* species, its preparation method, and its applications. The high-concentration carbon dioxide-tolerant *Synechococcus* species includes a CRISPR / dCas9 plasmid, a gRNA plasmid, and a knockout ω plasmid. This invention enables the growth of PCC7942 under high CO2 conditions by transcribedly activating PEPC using the CRISPR / dCas9 system. Furthermore, the fusion between the ω subunit of RNAP and a Cas9 nuclease mutant that binds to the upstream promoter region allows for programmable transcriptional activation. By knocking out the ω subunit, the CRISPR / dCas9 transcriptional regulation system is used to optimize the microalgal photosynthetic carbon fixation pathway, thereby improving the growth and carbon fixation efficiency of microalgae under high CO2 conditions.

The invention provides an application of Exifenone in preparation of a medicine for treating diabetic retinopathy, the Exifenone can be combined near a K412 lactic acid modification site of HDAC1, so that the lactic acid modification of the K412 site of the HDAC1 is specifically inhibited, the deacetylation activity of the HDAC1 to H3K56 is enhanced, the transcriptional activation of VEGFA (vascular endothelial growth factor A) mediated by H3K56ac and pathological angiogenesis are inhibited, and the effect of treating diabetic retinopathy is achieved. Further, vascular pathological injury caused by diabetic retinopathy is improved. The application provides a new target and a candidate drug for clinical treatment of diabetic retinopathy. The research result of the application provides experimental evidence for the epoxybenzophenone to be used for clinical specific treatment of diabetic retinopathy.

To provide methods and materials for genetically engineering methylotrophic yeast.SOLUTION: The present invention provides a methylotrophic yeast cell comprising a recombinant nucleic acid molecule, wherein the recombinant nucleic acid molecule comprises an exogenous nucleic acid encoding a transcriptional activator operably linked to at least one methanol-inducible promoter element.SELECTED DRAWING: None

The invention belongs to the technical field of kit detection, discloses a chromatin modification detection kit for regulating ATIC expression through PRMT8, and discovers the specific expression and cancer promotion function of arginine methyltransferase PRMT8 and purine de novo synthesis key enzyme ATIC in triple negative breast cancer for the first time. The specific biological effect of the PRMT8 in the triple negative breast cancer is researched for the first time, and the PRMT8 promotes ATIC transcriptional activation by mediating phosphorylation of an ATIC promoter region H4R3me2a and an effect protein TDRD3 of the PRMT8. The important function of arginine methyltransferase PRMT8 in a tumor purine metabolic pathway is found by gradually exploring the transcriptional activation process of purine metabolic rate-limiting enzyme ATIC, the post-translational modification function of histone is associated with the activation of the breast cancer purine metabolic pathway, and a new theoretical basis is provided for treating breast cancer by targeting PRMT8.

Provided are DNA-targeting systems, such as CRISPR-Cas / guide RNA (gRNA) systems, that bind to or target a target site in a gene or regulatory element thereof in a stem cell. In some embodiments, the provided DNA-targeting systems promote transcriptional activation of genes that promote lymphoid cell differentiation. In some embodiments, the provided DNA-targeting systems promote differentiation of hematopoietic progenitor cells (HPCs) into a differentiated population of cells. In some embodiments, the differentiated population of cells are lymphoid progenitor cells. In some embodiments, the differentiated population of cells are lymphoid cells. Also provided herein are methods and uses related to the provided compositions, for example in facilitating lymphoid cell differentiation without the provision of extrinsic differentiation signals.

The present application relates to a kind of FOXM1 protein phosphorylation modification interfering peptide and its application, belong to the field of biological medicine technology. Specifically, the present application is found in research, the transcriptional activity of FOXM1 protein depends on the condensate formed by its polymerization, destroying the polymerization of FOXM1 can effectively inhibit the occurrence and development of tumor, and the phosphorylation of the 376th amino acid of FOXM1 protein can also inhibit polymerization to some extent, thereby inhibiting the transcriptional activation of FOXM1 protein. Therefore, the present application designs an interfering peptide, and introduces phosphorylation modification in appropriate position, obtains a kind of phosphorylation modification interfering peptide FIP4 targeted to FOXM1 protein polymerization domain, the interfering peptide plays a significant role in inhibiting the transcriptional activation of FOXM1 protein in cell and animal in vivo, and can be used for treating tumor-related diseases.

The present disclosure relates generally to manipulation of chimeric polypeptide signaling to reduce cancer cell proliferation, and in particular to a new class of receptors engineered to (i) bind to a ligand displayed on the surface of a target cell, and (ii) have a synthetic transmembrane domain. The disclosure also provides compositions and methods useful for producing such receptors, nucleic acids encoding such receptors, host cells genetically modified with the nucleic acids, and methods for modulating cell activity and / or for the treatment of various diseases, such as cancer.

The invention discloses a SbASR4 gene for regulating and controlling plant salt stress tolerance as well as an encoding protein and application thereof. The SbASR4 gene is derived from Sorghum baicola, and the encoded protein is simultaneously positioned in a cell membrane and a cell nucleus, does not have transcriptional activation activity, and is a negative regulation factor of plant salt stress response. The overexpression of the gene can significantly increase the sensitivity of the plant to salt and ABA, and the silence or knockout of the gene can greatly improve the salt tolerance of the plant. Mechanism research shows that the SbASR4 protein directly interacts with an SOS signal channel core kinase SOS2 in a plant body, and the SOS channel is negatively regulated through the interaction, so that the expression of a downstream ion transport gene is inhibited. The invention provides a method for improving the salt tolerance of plants by reducing the expression of SbASR4 or destroying the interaction of SbASR4 and SOS2, related genetic materials and products, and a brand new target and an effective strategy are provided for salt-resistant breeding of crops.

The invention relates to the technical field of biological medicine, in particular to a truncated variant of a nuclear receptor LBD region and application of the truncated variant. According to the truncated variant of the nuclear receptor LBD structural domain provided by the invention, at least one amino acid is deleted at the C terminal. The ER, PR and GR truncated variants can induce transcriptional activation of a downstream signal channel under the condition that NTD is deleted, and a brand new treatment target is provided for patients with cancers such as prostatic cancer, breast cancer, endometrial cancer, acute lymphocytic leukemia, lymphoma, pancreatic cancer and lung cancer.

Provided herein are plants, plant parts, a population of plants or plant parts, and plant products (e.g., seed composition, protein composition) comprising reduced activity of a protein-related polypeptide [e.g., stomatal cytokinesis defective 2 (SCD2), SCD2A, SCD2B, response to dehydration 22 (RD22), glucuronidase 3 (GUS3), GUS3A, glycosyl hydrolase family 10 protein B (GH10B), protein phosphatase 2A beta subunit (PP2AB), PPA2BA, PP2ABAB, alpha / beta-hydrolases superfamily protein (ABH), ABHA, ABHB, calmodulin-binding transcription activator protein 2 (CAMTA2), CAMTA2A, CAMTA2B, cinnamyl-alcohol dehydrogenase (CAD1), beta-ketoacyl reductase 1 (KCR1), KCR1A, or KCR1B], and compositions and methods of producing such plants and plant parts. The plants, plant parts, population of plants or plant parts, or plant products can have a genetic mutation that reduces the protein-related polypeptide activity, which can be one located at least partially in a protein-related gene (e.g., CAD1) or its homolog or in its regulatory region, and can have increased protein content and / or white flake protein content.