The present application relates to the technical field of Parkinson's
disease model construction of
zebrafish, and specifically discloses a method for constructing a Parkinson's
disease model of
zebrafish induced by
artificial sweetener neotame and application thereof, and the technical points are as follows: the method comprises the following steps: mixing
neotame with water to obtain a
neotame aqueous solution mother liquor with a
mass concentration of 1 g / L; diluting the
mother liquor into a working solution with a
mass concentration of 1-100 mg / L by using breeding water; and breeding
zebrafish in the working solution for 4 weeks to obtain a Parkinson's
disease animal model of zebrafish induced by neotame. The present application also provides a model constructed by the method, an evaluation method of the model and application thereof. The model can be used for research on
nervous system diseases, screening of neotame
neurotoxicity, evaluation of neotame
neurotoxicity in food and high-
throughput screening of anti-Parkinson drugs.