The invention discloses a beef-containing chilli sauce and a preparation method thereof. The beef-containing chilli sauce is prepared through using raw materials comprising, by weight, 10-15 parts of beef, 45-50 parts of Hot Pepper, 1-3 parts of Chinese waxgourd seed, 8-12 parts of soybean paste, 2-5 parts of seville orange flower, 10-15 parts of Moringa tree leaf, 1-2 parts of Radix Ophiopogonis, 1-1.5 parts of dried orange peel, 1-3 parts of Rhizoma Atractylodis Macrocephalae, 1-3 parts of Atractylodes lancea, 1-2 parts of cassia twig, 1-2 parts of Poria cocos powder, 2-5 parts of ginger, 3-5 parts of garlic, 0.5-1 part of yellow rice wine, 2-3.5 parts of monosodium glutamate, 5-10 parts of salt, 0.1-0.2 parts of hawthorn seed powder, 01-0.3 parts of Myristica fragrans powder, and a proper amount of plant oil. The Moringa tree leaf is pulped, an obtained pulp is mixed with powder to prepare a paste, and the paste is mixed with beef granules to form a starchy sauce, so the chilli sauce has the fragrance of beef and has increased fresh and hot mouthfeel; and beef is immersed in traditional Chinese medicinal components having the spleen invigorating, qi tonifying, stomach nourishing and stomach strengthening functions, and then is mixed with the preserved Hot Pepper to prepare the sauce, so the sauce has the characteristics of strong and lasting fragrance, abundant nutrition, delicious taste, and seasoning and healthcare functions, is a scientific, healthy, delicious and safe edible seasoning sauce, and can be eaten by all populations.