The utility model relates to the field of celery seed production. The isolation device for celery seed production comprises a bottom plate, shielding plates are installed at the two ends of the bottom plate, a plurality of detachable side plates are installed between the shielding plates, a cover plate is installed above the side plates, and ventilation nets are arranged on the side faces of the shielding plates. A first sliding groove is formed in one side, in contact with the shielding plate, of the side plate, a fixing column is movably installed in the first sliding groove, a connecting groove is formed in the fixing column, the bottom plate, the shielding plate, the detachable side plate, a cover plate and a breathable net are included, and flexible fixing and releasing of the side plate are achieved through the sliding groove, the fixing column, the connecting column, a rotating ring, a shifting plate, a rotating shaft and other structures; a user can easily operate the shifting plate, and the rotating ring is driven to rotate through the rotating shaft, so that the fixing of the side plate is controlled, the size of an isolation space is convenient to adjust, the seed production space is convenient to adjust, transportation and storage are convenient, the operation process is simplified, the working efficiency is improved, and subsequent cleaning and maintenance are convenient.