The invention relates to a preparation method of flavored nutritional barbecue sauce. The barbecue sauce is prepared from the following raw materials: Chinese prickly ash, minced garlic, white sesames, ginger grains, salt, celery seed powder, peanut powder, cortex albiziae powder, walnut powder, red chili powder, onion powder, carrot powder, tomato sauce, maltodextrin, modified starch, glucose, fermented soybean sauce, soybean sauce, cumin essential oil, chicken powder, mutton powder, yellow rice or millet wine, white spirit, fish sauce, beef powder, beef tallow, gourmet powder, seafood powder, cumin powder and water. The barbecue sauce prepared by the preparation method has a high natural feeling, a rich taste and a delicious taste, and can be used for effectively stimulating appetite of people.