Method for preparing composite flavoring agent and product prepared by same
A product, a purebred technology, applied in the fields of fermentation, condiments, and food microbes, can solve problems such as low memory and mental stimulation, and achieve the effect of delicious taste, sufficient nutrient consumption, and simple preparation methods
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Embodiment 1
[0012] A method for preparing a paste-like compound flavor-enhancing base, including the processes of fermentation mixing, microfiltration sterilization and concentration, wherein the fermentation mixing is to ferment and cultivate pure Corynebacterium ammoniagenes until the concentration of disodium inosinate IMP reaches After 3%, it is mixed with Corynebacterium glutamicum or Bacillus subtilis fermentation broth that is fermented until the concentration of glutamic acid in the fermentation broth reaches 5% in a mass ratio of 5% through a sterilized pipeline, and the fermentation conditions are adjusted to continue fermentation to obtain a fermentation mixture liquid; the microfiltration sterilization refers to heating and sterilizing the fermentation mixture at a temperature of 60 ± 5 °, and the fermentation filtrate is obtained by microfiltration membrane sterilization; the concentration (or concentrated drying) refers to the fermentation filtrate Concentrate at 50-90°C for ...
Embodiment example 2
[0017] A method for preparing a paste-like compound flavor-enhancing base. The difference from Example 1 is that the fermentation mixture is to ferment and cultivate Corynebacterium ammoniagenes until the concentration of disodium inosinate IMP reaches 7%, and then mix with pure-bred Ferment to the Corynebacterium glutamicum or Bacillus subtilis fermented liquid that glutamic acid concentration reaches 12% in the fermented liquid is 20% by mass ratio and mixes through sterilizing pipeline, adjusts fermenting condition and continues fermenting to obtain fermented mixed liquid; Bacteria refers to heating and sterilizing the fermentation mixture at a temperature of 60±5°C, and sterilizing the fermentation filtrate through a microfiltration membrane; the concentration (or concentrated drying) refers to concentrating the fermentation filtrate at a negative pressure of 50-90°C -8 times, packaged as a paste product; or continue to dry the concentrate to obtain a powder product. After...
Embodiment 3
[0022] A preparation method of paste-like compound taste-enhancing base, the difference from Example 1 is: after the concentration of IMP reaches 10%, it is fermented with Corynebacterium glutamicum or subtilis with pure species until the concentration of glutamic acid in the fermentation liquid reaches 15%. The Bacillus fermentation broth is mixed according to the mass ratio of 35% through a sterilized pipeline, and the fermentation conditions are adjusted to continue fermentation to obtain a fermentation mixture; The fermentation filtrate is obtained by microfiltration membrane sterilization; the concentration (or concentrated drying) means that the fermentation filtrate is concentrated by 3-8 times under negative pressure at 50-90°C, and packaged to obtain a paste product; or the concentrated solution is continuously dried to obtain a powder product. After detection by Agilent high performance liquid chromatography and high performance gas chromatography, its main nutrition...
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