8 results about "Catalpa" patented technology

The application provides a DNA extraction method for leaves of a plant of the genus Catalpa, and relates to the technical field of DNA extraction of plant leaves. The method is based on the CTAB method, and a lysis solution is optimized so that the extraction method is suitable for the plant of the genus Catalpa. The DNA extraction method for leaves of the plant of the genus Catalpa has the beneficial effect of high extraction rate.

This invention provides a method for regulating nitrogen absorption and utilization in catalpa trees. CbuLBD9 This invention relates to genes and their applications, belonging to the field of forest tree genetic engineering breeding technology. CbuLBD9 The nucleotide sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence it encodes is shown in SEQ ID No. 2. This invention utilizes silencing... CbuLBD9 This gene significantly promotes the elongation and growth of the Catalpa tree root system and enhances the absorption and utilization of nitrogen by the plant roots. This invention provides an important candidate gene for genetic improvement related to efficient nitrogen utilization in Catalpa trees, and has broad application prospects in forest tree genetic engineering breeding and the development of Catalpa tree clones.

The invention provides a composition with sunscreen and repairing effects as well as a preparation method and application thereof, and relates to the technical field of daily necessities. The composition with the sun-screening and repairing effects is prepared from the following components in parts by weight: 10 to 20 parts of pongamia pinnata seed oil, 10 to 20 parts of magnolia liliiflora bud extract, 1 to 10 parts of aster purpurea extract, 5 to 10 parts of catalpa bark / leaf extract, 1 to 3 parts of Chile strawberry extract and 2 to 5 parts of dendrobium fimbriatum leaf extract. The solid content of the magnolia liliiflora bud extract is 3 to 5 mg / mL. The six components are compatible with one another, so that ultraviolet rays can be effectively resisted, the skin red after exposure can be relieved, the skin barrier can be repaired, the health state of the skin can be recovered, the composition can be used for preparing sun-screening and repairing skin care products, and the composition does not irritate the skin, does not block pores, is suitable for sensitive skin, feels fresh and comfortable during use, is high in safety and has a good application prospect.

The application provides a catalpa tree age prediction model, and relates to the technical field of predicting the age of a catalpa tree with a grade of three or more. The prediction model is as follows: y=0.013e 6.17x +53.616, wherein y is the age of the catalpa tree, and x is the average telomerase activity value of the leaves in the south upper part of the tree. The prediction model provided by the application can be used for monitoring the age of a catalpa tree with a grade of three or more, and the maximum error is 10%.

This invention provides a method for detecting telomerase activity in plants of the genus *Catella*, belonging to the field of plant telomerase detection technology. Specifically, it includes the following steps: telomerase solution samples are added to a premixed solution, and DEPC-treated water is added to make up the volume. This premixed solution includes TRAP buffer, dNTP mixture, leader primer, reverse primer, and Taq polymerase. PCR amplification is then performed, with the following amplification program: PCR extension at 26 °C for 30 min; pre-denaturation at 95 °C for 2 min; 30 cycles of 94 °C for 30 s, 58 °C for 30 s, and 72 °C for 60 s; 72 °C for 10 min; and storage at 4 °C. After amplification, the PCR amplification products are subjected to agarose gel electrophoresis, stained with SYBR Green I staining solution, and observed under a gel imaging system. This invention proposes a method for detecting telomerase activity in plants of the genus *Catella*, applicable to various plants in the genus, and the testing process is safe and reliable.

The invention provides a CbuLBD9 gene for regulating nitrogen absorption and utilization of catalpa bungei and application of the CbuLBD9 gene, and belongs to the technical field of forest tree genetic engineering breeding. The nucleotide sequence of the CbuLBD9 gene disclosed by the invention is as shown in SEQ ID No. 1, and the amino acid sequence coded by the CbuLBD9 gene is as shown in SEQ ID No. 2. By silencing the CbuLBD9 gene, the elongation and growth of the root system of the catalpa bungei can be obviously promoted, and the absorption and utilization of the plant root system to nitrogen can be improved. The invention provides an important candidate gene for genetic improvement related to efficient nitrogen utilization of catalpa bungei, and has a wide application prospect in the aspects of forest tree genetic engineering breeding and catalpa bungei clonal development.

The utility model belongs to the field of catalpa leaf feed, disclose a kind of catalpa leaf solar drying room, room body, room body is set on ground, the side of room body is provided with second window, the top surface of room body is provided with roof, the inside of room body is provided with house beam, temperature and humidity inceptor is installed on house beam, cooling structure, cooling structure is set on roof top, the top of roof is provided with when indoor temperature is too high can automatically unfold and sunshade's reel rod, the side of reel rod is provided with sponge block along with reel rod movement and wipe roof top surface, the other end of two first transmission strips is turned on and drives two second transmission strips to rotate and unfold, moving block drives the sunshade curtain that is released to stretch, sunshade curtain covers roof, ensure that when cooling, sun no longer bakes, cooling speed is faster, catalpa leaf short time will not cell wall rupture.

The application belongs to the technical field of forest tree genetic engineering breeding, and more particularly relates to a CbuLBD19 gene for regulating root development and nitrogen absorption and utilization of a Catalpa bungeana and application thereof. The nucleotide sequence of the CbuLBD19 gene is shown as SEQ ID No. 7, and the amino acid sequence encoded by the CbuLBD19 gene is shown as SEQ ID No. 8. The application finds that overexpression of the CbuLBD19 gene can lead to reduction of total root length and root biomass of the Catalpa bungeana. Meanwhile, the overexpression can lead to reduction of net absorption rate of nitrate ions, nitrate reductase (NR) activity and amino acid content in the root system, and the Catalpa bungeana shows a comprehensive decrease in nitrogen metabolism. The function of the CbuLBD19 gene in negative regulation of nitrogen absorption and utilization of the Catalpa bungeana is clarified, and a technical route for improving nitrogen absorption and utilization of the Catalpa bungeana by reducing expression of the CbuLBD19 gene is provided.