31 results about "Liriodendron tulipifera" patented technology

The present invention relates to a pharmaceutical composition containing, as an active ingredient, a Liriodendron tulipifera L. bark extract for treating chronic myelogneous leukemia. More specifically, the present invention relates to the pharmaceutical composition, containing, as the active ingredient, the Liriodendron tulipifera L. bark extract useful in selectively inhibiting mutant enzyme T315I from a mutation of a BCR-ABL fusion gene that causes chronic myelogenous leukemia, and to a use of epi-Tulipinolide which is an active ingredient of the composition.

The invention discloses a medium for promoting somatic embryogenesis of Liriodendron chinensis by using γ-aminobutyric acid and its application, belonging to the technical field of plant tissue culture. The culture medium for somatic embryogenesis of hybrid Liriodendron chinensis of the present invention is based on 3 / 4MS+VC 5mg / L+sucrose 30g / L as the base medium, and γ-aminobutyric acid of 0~10mg / L is added; the added γ ‑GABA can promote the generation of somatic embryos in the callus of Liriodendron chinensis, accelerate the development of somatic embryos, and improve the efficiency of somatic embryogenesis. The culture medium for somatic embryogenesis of Liriodendron chinensis provided by the present invention is applied in somatic embryogenesis and tissue culture of Liriodendron chinensis, the number of somatic embryogenesis is large, the speed is fast, and the quality is high, and the number of somatic embryos induced is very high. Significantly higher than that of the control group, the number of lateral roots and root length were significantly higher than that of the control group, and an average of 20 regenerated plants can be formed per callus, which has high practical application value.

The invention provides a primer pair for amplifying the DNA barcode of Liriodendron and an identification method for Liriodendron and its provenance, belonging to the technical field of plant molecular identification. The primer pair for amplifying the DNA barcode of Liriodendron includes upstream amplification An amplification primer and a downstream amplification primer; the sequence of the upstream amplification primer is shown in SEQ ID No.1; the sequence of the downstream amplification primer is shown in SEQ ID No.2. Liriodendron tulipifera identification method provided by the invention comprises the following steps: 1) extracting the whole genome DNA of the sample to be detected; 2) using the whole genome DNA as a template, performing PCR amplification with the DNA barcode primer pair to obtain an amplified product 3) Sequencing the amplified product, obtaining the specific sequence information of the amplified product, comparing the specific sequence information with the standard sequence of the Liriodendron tulipifera DNA barcode, and determining the tree species and regional provenance of the sample to be tested, the The method is simple in operation and high in accuracy.

The present invention provides a method for separating and preparing a therapeutic agent for chronic myelogenous leukimeia capable of effectively inhibiting growth of T315I, which is known as a mutation of a BCR-ABL fusion gene that causes chronic myelogenous leukemia, comprising the steps of: 1) preliminary extracting by adding ethyl acetate to finely chopped bark of Liriodendron tulipifera L., adding butanol to an extracted fluid for layer-separating an ethyl acetate layer and a butanol layer, removing the butanol layer, and decompression-condensing the remainder to obtain a crude extract; and 2) adding a C1-C3 lower alcohol aqueous solution and n-hexane to the obtained crude extract, removing lipids and water insoluble material dissolved in the n-hexane layer, and obtaining and separating a lower alcohol aqueous solution layer to separate and refine highly pure epi-Tulipinolide and costunolide.

The invention discloses a method for identifying the type of descendant tree species of Liriodendron tulipifera forest and a special kit thereof, the method comprising: 1) extracting leaf DNA of the descendant tree species; 2) performing SSR-PCR detection; 3) judging the descendant tree species, Electrophoresis can specifically amplify the 112bp product as Liriodendron chinensis, the 124bp product that can be specifically amplified is Liriodendron tulipifera, and the 112bp and 124bp products that can be specifically amplified are hybrid Liriodendron tulipifera. The method for identifying the tree species type of the descendants of Liriodendron forest stand of the present invention has the advantages of good repeatability and stability, and can effectively identify the tree species types of the descendants of Liriodendron tulipiflora in natural forests and artificial forests, especially those in the seedling stage. The offspring of the natural forest plantation of Liriodendron can also identify whether the seeds in the seed garden are Chinese species, North American species, or hybrid species. The speed is fast, the efficiency is high, the result is accurate, and it has good practicability.

The invention discloses a somatic embryo all-solid cultivation plant regeneration method of hybridized liriodendron tulipifera by deionized formamide. The method includes the steps of: 1) inducing embryogenic callus of hybridized liriodendron tulipifera; 2) dark-culturing the embryogenic callus of the hybridized liriodendron tulipifera at 24 DEG C with a basic culture medium of 3 / 4MS + 5 mg / L of VC + 30 g / L of saccharose + 0.01-1 mg / L of the deionized formamide; 3) moving the material to a tissue culture bottle in a light culturing room, and continuously culturing the material with 3 / 4MS as a basic culture medium to obtain a regenerated plant; and 4) domesticating and transplanting the regenerated plant. Through the all-solid cultivation method, two somatic embryo, suspension cultivation and plate cultivation, during a somatic embryo generation process are omitted, thereby optimizing and enriching the somatic embryo regeneration system of the hybridized liriodendron tulipifera and further more high-effectively and conveniently obtaining high-quality somatic embryo regeneration regenerated plants. The method can establish the all-solid culture medium somatic embryo generation system, which is stable and high-frequent, is simple in operation and has excellent nursery stock resistance, of the hybridized liriodendron tulipifera, and provides references for establishing a more high-effective genetic transformation receptor system of the hybridized liriodendron tulipifera.

The invention discloses a Liriodendron tulipifera transcription factor LcbHLH52 gene and an application thereof, belonging to the technical field of plant genetic engineering. The present invention analyzes the bHLH family sequence of Liriodendron tulipifera through bioinformatics tools, and then successfully clones the LcbHLH52 transcription factor gene through sequencing and homologous comparison. Construct an overexpression vector for the cloned LcbHLH52 transcription factor gene, transform Arabidopsis thaliana, and obtain T1 generation seeds; put LcbHLH52 transgenic Arabidopsis thaliana T2 generation plants and wild-type Arabidopsis plants into 4°C low temperature treatment for 3 days at the same time, and the phenotype It was observed that the growth of transgenic plants was less affected by low temperature stress, while the growth of wild-type plants was inhibited, and the leaves had wilting phenomenon. The results show that the LcbHLH52 gene of Liriodendron thaliana can enhance the tolerance of Arabidopsis plants to low temperature stress, and has important application value in molecular breeding for improving low temperature stress of plants.