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31 results about "Liriodendron tulipifera" patented technology
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Efficient transient gene expression method for hybrid liriodendron taking mesophyll protoplast as receptor
InactiveCN106480085AQuick and easy operation platformVector-based foreign material introductionMagnoliaceaeGreen fluorescent protein
The invention discloses an efficient transient gene expression method for hybrid liriodendron taking mesophyll protoplast as a receptor. The efficient transient gene expression method for the hybrid liriodendron taking mesophyll protoplast as the receptor comprises the following steps: preparation of the mesophyll protoplast of the hybrid liriodendron, preparation of plasmids, conversion and observation. The protoplast of mesophyll cells of the hybrid liriodendron is used as a receptor system of genetic transformation, by a PEG mediated method, green fluorescent protein (GEP) genes are used as reporter genes, and efficient transient expression of GFP genes is detected through a fluorescence microscope. The leaf blade of the hybrid liriodendron is used as a source of the protoplast, materials are taken conveniently, vitality is high, and an established transient expression system can provide a rapid and convenient operation platform for research of gene functions of liriodendron tulipifera and magnoliaceae.
Owner:NANJING FORESTRY UNIV
Liriodendron tulipifera cutting propagation method
The invention discloses a liriodendron tulipifera cutting propagation method. The liriodendron tulipifera cutting propagation method comprises the following steps that healthy and strong trees free of diseases and insect pests are selected and 1-2a thick and solid branches are sheared, wherein the length of shoots ranges from 13 cm to 16 cm, and each shoot has 2-3 buds; cuts of cuttings are flat, the distance from each upper cut to a nearest bud is 3 cm, and the distance from each lower cut to a nearest bud is 0.5-1.0 m; the upper physiological ends of bundled cutting shoots are upwards soaked in flowing water before cuttage, the upper cuts are not submerged in water, and the soaking time is 5-7 days; therefore, rooting can be promoted, spouting is advanced, and the growing amount of nursery stocks can be further increased; the cutting shoots are exposed out of the ground for 1-2 cm when the cuttings are buried in soil in a straight cutting mode, and one-bud exposure is preferable; the planting row space is 20 cm*30 cm, watering is performed after cuttage, and the cuttings are covered by using plastic films. By means of the liriodendron tulipifera cutting propagation method, cutting rot can be prevented, cutting rooting and propagation can be further accelerated, and the survival rate of propagated liriodendron tulipifera cuttings is greatly improved.
Owner:SHUYANG COUNTY SUBEI FLOWER
Method for acquiring two-dimensional electrophoretogram of nectar protein of liriodendron tulipifera by aid of two-dimensional electrophoresis system
InactiveCN106198691AImprove extraction efficiencyHigh resolutionPreparing sample for investigationMaterial analysis by electric/magnetic meansLiriodendron tulipiferaProtein molecules
The invention discloses a method for acquiring a two-dimensional electrophoretogram of nectar protein of liriodendron tulipifera by the aid of a two-dimensional electrophoresis system. After a nectar sample is filtered by an ultramicro filter membrane, the nectar protein is concentrated and purified by a protein ultrafiltration and concentration purification column, the concentration of the purified and concentrated nectar protein is detected with a Bradford method, the molecular weight distribution range of the nectar protein of the liriodendron tulipifera is pre-detected through one-dimensional lauryl sodium sulfate polyacrylamide gel electrophoresis, finally, proper standard protein and a gel strip with a proper length and pH range are selected according to a one-dimensional electrophoresis result, the nectar protein of the liriodendron tulipifera is separated with the two-dimensional electrophoresis system, and the two-dimensional electrophoretogram is acquired. The method has the characteristics that the operation is simple, the protein extraction rate is high and few interfering substances exist and is suitable for special materials such as liquid samples, the high-quality two-dimensional gel electrophoretogram with high resolution ratio, clear protein points, larger numbers, uniform distribution and clear background can be obtained, and the experiment repeatability and the stability are good.
Owner:NANJING FORESTRY UNIV
Liriodendron tulipifera hardwood cutting method
InactiveCN107087493AFast rootingHigh rooting rate of cuttingsPlant cultivationCultivating equipmentsAxillary budLiriodendron tulipifera
The invention provides a liriodendron tulipifera hardwood cutting method. The method comprises the steps of cutting selection, cutting bed preparation, cutting slip treatment, cutting, cutting aftertreatment and transplantation; in the cutting selection step, liriodendron tulipifera hardwood is selected as a cutting test material in spring, cuttings are 0.6-0.7 cm thick and cut into sections, and each section is 18-22 cm long and has 2-3 axillary buds and 2-4 leaves. In the cutting bed preparation step, an anti-corrosion cutting medium is uniformly spread all over a seedbed, the thickness of the medium is 20-22 cm, water is sprayed once, and the humidity of the medium is 50%-55%. In the cutting step, full-automatic atomizing cutting is adopted, the cutting depth is 5 cm, and the cutting distance between seedlings is 30*30 cm. The cutting survival rate is 99.2%-99.7% after the method is used for cutting cultivation of liriodendron tulipifera.
Owner:WEIFANG VOCATIONAL COLLEGE
Pharmaceutical Composition Containing as Active Ingredient Extract From Bark of Liriodendron Tulipifera
InactiveUS20150011623A1Good effectImprove bioavailabilityBiocideDigestive systemLiriodendron tulipiferaBULK ACTIVE INGREDIENT
The present invention relates to a pharmaceutical composition containing, as active ingredients, epitulipinolide and costunolide extracted from the bark of Liriodendron tulipifera. More specifically, the present invention relates to a pharmaceutical composition for treating the gastrointestinal tract, the composition containing, as active ingredients, epitulipinolide and costunolide, which are the active ingredients of the extract from the bark of Liriodendron tulipifera, and to the use thereof as a therapeutic agent for gastrointestinal diseases.
Owner:CHO DANG PHARM
Traditional Chinese medicament for treating wind-phlegm in lung type asthma
InactiveCN104027760ASafe and effective treatmentRespiratory disorderPlant ingredientsMedicinal herbsLiriodendron tulipifera
Owner:YANTAI RUIZHI BIOLOGICAL MEDICINE SCI & TECH
Identification method for types of descendant tree species of Liriodendron forest
ActiveCN108660250AGood repeatabilityImprove stabilityMicrobiological testing/measurementLiriodendron tulipiferaNatural forest
The invention discloses an identification method for the types of descendant tree species of Liriodendron forest and a special kit thereof. The method comprises the following steps: 1) extracting leafDNA (Deoxyribonucleic Acid) of the descendant tree species; 2) performing SSR-PCR (Simple Sequence Repeat-Polymerase Chain Reaction) detection; 3) determining the descendant tree species, wherein thedescendant tree species which can specifically amplify a product of 112bp by electrophoresis are Liriodendron chinense (Hemsl.) Sarg., the descendant tree species which can specifically amplify a product of 124bp by electrophoresis are Liriodendron tulipifera Linn., and the descendant tree species which can specifically amplify products of 112bp and 124bp are hybrid of Liriodendron chinense and Liriodendron tulipifera. The identification method for the types of the descendant tree species of Liriodendron forests disclosed by the invention has the advantages of high repeatability and high stability, can effectively identify the types of descendant tree species of Liriodendron natural forests and artificial forests, particularly the descendant trees of Liriodendron natural forests and artificial forests at a seedling stage, and can identify whether seeds of seed orchards are Liriodendron chinense (Hemsl.). seeds, Liriodendron tulipifera seeds or seeds of hybrid of Liriodendron chinenseand Liriodendron tulipifera. The identification method has the advantages of fastness, high efficiency, accurate result and very high practicality.
Owner:NANJING FORESTRY UNIV
Method for screening SNP markers of Liriodendron genome
ActiveCN108754005AQuality improvementValid statement accuracyMicrobiological testing/measurementF1 generationLiriodendron tulipifera
The invention discloses a method for screening SNP markers of a Liriodendron genome. F1-generation individuals obtained from Liriodendron tulipifera 'NK' and Liriodendron 'LS' by hybridization are taken as a material, a Liriodendron reference genome obtained by sequencing is taken as the basis, PCR amplification primers are designed by Primer 5.0 software, amplification efficiency and specificityof the primers are verified, 27 candidate SNP loci are selected for primer design, a screening result is detected by agarose gel electrophoresis after PCR amplification, provenance material are verified, and finally, the SNP markers of the Liriodendron genome are obtained. 27 SNP loci are selected from a Liriodendron genome database for primer synthesis, products are subjected to PCR amplificationdetection and sent out for sequencing, a result shows that all the loci are real SNP loci, which means that the SNP loci are reliable in quality, used standards are better, and the obtained high-quality SNP markers can be applied to follow-up work such as genetic linkage maps and the like.
Owner:NANJING FORESTRY UNIV
Dosage Form Containing Extract From Bark of Liriodendron Tulipifera as Active Ingredient
InactiveUS20150011622A1Easily solubilizedHigh dissolution rateBiocideDispersion deliveryLiriodendron tulipiferaActive ingredient
The present invention relates to an improved dosage form containing, as active ingredients, epitulipinolide and costunolide extracted from the bark of Liriodendron tulipifera and, more specifically, to a dosage form for treating the gastrointestinal tract, the dosage form containing, as active ingredients, epitulipinolide and costunolide, which are the active ingredients of the extract from the bark of Liriodendron tulipifera.
Owner:CHO DANG PHARM
Somatic embryo all-solid cultivation plant regeneration method of hybridized liriodendron tulipifera by deionized formamide
ActiveCN106212287AImprove induction abilityEfficient and convenient accessHorticulture methodsPlant tissue cultureLiriodendron tulipiferaBottle
The invention discloses a somatic embryo all-solid cultivation plant regeneration method of hybridized liriodendron tulipifera by deionized formamide. The method includes the steps of: 1) inducing embryogenic callus of hybridized liriodendron tulipifera; 2) dark-culturing the embryogenic callus of the hybridized liriodendron tulipifera at 24 DEG C with a basic culture medium of 3 / 4MS + 5 mg / L of VC + 30 g / L of saccharose + 0.01-1 mg / L of the deionized formamide; 3) moving the material to a tissue culture bottle in a light culturing room, and continuously culturing the material with 3 / 4MS as a basic culture medium to obtain a regenerated plant; and 4) domesticating and transplanting the regenerated plant. Through the all-solid cultivation method, two somatic embryo, suspension cultivation and plate cultivation, during a somatic embryo generation process are omitted, thereby optimizing and enriching the somatic embryo regeneration system of the hybridized liriodendron tulipifera and further more high-effectively and conveniently obtaining high-quality somatic embryo regeneration regenerated plants. The method can establish the all-solid culture medium somatic embryo generation system, which is stable and high-frequent, is simple in operation and has excellent nursery stock resistance, of the hybridized liriodendron tulipifera, and provides references for establishing a more high-effective genetic transformation receptor system of the hybridized liriodendron tulipifera.
Owner:NANJING FORESTRY UNIV
Liriodendron transcription factor LcbHLH16421 gene and application thereof
ActiveCN112725356AGrowth inhibitionLow temperature stress has little effect onPlant peptidesFermentationBiotechnologyLiriodendron tulipifera
The invention discloses a liriodendron transcription factor LcbHLH16421 gene and application thereof, and belongs to the technical field of plant genetic engineering. The liriodendron tulipifera bHLH family sequence is analyzed through a bioinformatics tool, and then the LcbHLH16421 transcription factor gene is obtained through cloning through sequencing and homologous comparison. An overexpression vector for the LcbHLH16421 gene is constructed, and arabidopsis thaliana is converted to obtain T1-generation seeds; LcbHLH16421 transgenic arabidopsis thaliana T2-generation plants and wild plants are treated at the low temperature of 4 DEG C for 3 days at the same time, phenotypic observation shows that the influence of low-temperature stress on the growth of the transgenic plants is small, the growth of the wild plants is inhibited, and leaves are withered. The result shows that the liriodendron tulipifera LcbHLH16421 gene can enhance the tolerance of an arabidopsis thaliana plant to low-temperature stress, and has important application value in molecular breeding for improving the low-temperature stress of the plant.
Owner:NANJING FORESTRY UNIV
A composition capable of improving the stress resistance and growth-promoting effect of somatic embryo seedlings of Liriodendron chinensis and its application
InactiveCN107927007BImprove drought resistancePromote growthBiocidePlant growth regulatorsBiotechnologyLiriodendron
The invention discloses a composition capable of improving stress resistance of hybrid liriodendron body embryo seedlings and realizing a promoting effect. The composition is prepared from 5 to 10 parts of wheat germ agglutinin, 5 to 10 parts of peanut agglutinin, 5 to 10 parts of sodium lignosulphonate, 5 to 10 parts of high-dispersion silicic acid, 10 to 20 parts of sodium dodecyl sulfonate and70 to 140 parts of kaolin. According to the composition disclosed by the invention, massive experiments are conducted for screening; the wheat germ agglutinin and the peanut agglutinin of which the weight ratio is 1 to 1 are creatively adopted as main active ingredients and matched with the sodium lignosulphonate, the high-dispersion silicic acid, the sodium dodecyl sulfonate and the kaolin with optimal use amounts. Experimental results show that the composition provided by the invention has the beneficial effects that the resistance of the hybrid liriodendron body embryo seedlings to adversetemperature environments of 4 DEG C low temperature and 40 DEG C high temperature is remarkably enhanced, and the drought resistance of the hybrid liriodendron body embryo seedlings can be obviously improved. The composition disclosed by the invention has the advantages of scientificity and reasonability in component matching, greenness, environmental protection, low toxicity and important practical application value.
Owner:HUAIYIN TEACHERS COLLEGE
Pharmaceutical composition containing liriodendron tulipifera l. extract for treating chronic myelogenous leukemia
InactiveCN105358171AApoptoticOrganic active ingredientsAntineoplastic agentsLiriodendron tulipiferaBCR-ABL Fusion Gene
Owner:CHO DANG PHARM
A kind of gene of transcription factor lcbhlh16421 of Liriodendron tulipifera and its application
ActiveCN112725356BGrowth inhibitionLow temperature stress has little effect onPlant peptidesFermentationBiotechnologyLiriodendron tulipifera
The invention discloses a gene of transcription factor LcbHLH16421 of Liriodendron tulipifera and its application, belonging to the technical field of plant genetic engineering. The present invention analyzes the sequence of the Liriodendron bHLH family through bioinformatics tools, and then clones and obtains the LcbHLH16421 transcription factor gene through sequencing and homologous comparison. An overexpression vector was constructed for the LcbHLH16421 gene, transformed into Arabidopsis thaliana, and T1 generation seeds were obtained; the LcbHLH16421 transgenic Arabidopsis T2 generation plants and wild-type plants were placed in a low temperature treatment at 4°C for 3 days at the same time, and the phenotype observation found that the growth of the transgenic plants was affected by the low temperature. The impact of stress was small, while the growth of wild-type plants was inhibited, and the leaves were wilting. The results show that the LcbHLH16421 gene of Liriodendron thaliana can enhance the tolerance of Arabidopsis plants to low temperature stress, and has important application value in molecular breeding for improving low temperature stress of plants.
Owner:NANJING FORESTRY UNIV
Liriodendron transcription factor LcbHLH02399 gene as well as expression protein and application thereof
ActiveCN114807166AGrowth inhibitionImprove tolerancePlant peptidesGenetic engineeringBiotechnologyLiriodendron tulipifera
The invention discloses a liriodendron transcription factor LcbHLH02399 gene as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The bHLH family sequence of liriodendron is analyzed through a bioinformatics tool, and then the LcbHLH02399 gene for coding the bHLH transcription factor is cloned through sequencing and homologous comparison. The method comprises the following steps: constructing an overexpression vector for an LcbHLH02399 gene, transforming hybrid liriodendron calluses, and carrying out liquid culture to obtain transgenic hybrid liriodendron; compared with phenotypes of LcbHLH02399 transgenic hybrid liriodendron plants and wild plants which are subjected to low-temperature treatment in an illumination incubator at 4 DEG C for 3 days, the influence of low-temperature stress on the growth of the transgenic plants is small, the growth of the wild plants is inhibited, and leaves are withered. The result shows that the liriodendron tulipifera LcbHLH02399 gene can enhance the tolerance of a liriodendron tulipifera plant to low-temperature stress, and has an important application value in molecular breeding for improving the low-temperature stress of the plant.
Owner:NANJING FORESTRY UNIV
Use of Liriodendron tulipifera or its extract in preparing medicine for reducing serum uric acid level and preventing and treating uric acid nephropathy
ActiveCN112704690BPromote excretionLower serum uric acid levelsSkeletal disorderUrinary disorderRenal glomerulusLiriodendron tulipifera
The invention relates to the application of Liriodendron tulipifera or its extract in the preparation of medicines for reducing serum uric acid level and preventing and treating uric acid nephropathy, which belongs to the field of medicine. One aspect of the present invention provides the application of Liriodendron tulipifera or its extract in the preparation of medicine for lowering blood uric acid level. On the other hand, the present invention also provides the use of Liriodendron tulipifera or its extract in the preparation of medicine for treating and / or preventing uric acid nephropathy. Animal experiments have confirmed that Liriodendron can significantly reduce serum uric acid levels, and at the same time improve the degree of renal tubular dilation, glomerulosclerosis and renal interstitial fibrosis in hyperuricemia mice. Further studies have shown that Liriodendron can reduce serum uric acid levels by promoting renal uric acid excretion. The application of the present invention can provide a new medication option for the clinical treatment of hyperuricemia and the resulting hyperuricemia nephropathy.
Owner:SICHUAN CREATION PHARM TECH CO LTD
A Medium for Promoting Somatic Embryogenesis of Liriodendron chinensis by Using γ-Aminobutyric Acid and Its Application
ActiveCN110301356BSpeed up the developmental processImprove production efficiencyHorticulture methodsPlant tissue cultureBiotechnologyLiriodendron tulipifera
The invention discloses a medium for promoting somatic embryogenesis of Liriodendron chinensis by using γ-aminobutyric acid and its application, belonging to the technical field of plant tissue culture. The culture medium for somatic embryogenesis of hybrid Liriodendron chinensis of the present invention is based on 3 / 4MS+VC 5mg / L+sucrose 30g / L as the base medium, and γ-aminobutyric acid of 0~10mg / L is added; the added γ ‑GABA can promote the generation of somatic embryos in the callus of Liriodendron chinensis, accelerate the development of somatic embryos, and improve the efficiency of somatic embryogenesis. The culture medium for somatic embryogenesis of Liriodendron chinensis provided by the present invention is applied in somatic embryogenesis and tissue culture of Liriodendron chinensis, the number of somatic embryogenesis is large, the speed is fast, and the quality is high, and the number of somatic embryos induced is very high. Significantly higher than that of the control group, the number of lateral roots and root length were significantly higher than that of the control group, and an average of 20 regenerated plants can be formed per callus, which has high practical application value.
Owner:NANJING FORESTRY UNIV
A primer pair for amplifying the barcode of Liriodendron tulipifera and the identification method of Liriodendron tulipifera and its provenance
ActiveCN110241246BIdentification is accurateEasy to operateMicrobiological testing/measurementDNA/RNA fragmentationLiriodendronMolecular identification
The invention provides a primer pair for amplifying the DNA barcode of Liriodendron and an identification method for Liriodendron and its provenance, belonging to the technical field of plant molecular identification. The primer pair for amplifying the DNA barcode of Liriodendron includes upstream amplification An amplification primer and a downstream amplification primer; the sequence of the upstream amplification primer is shown in SEQ ID No.1; the sequence of the downstream amplification primer is shown in SEQ ID No.2. Liriodendron tulipifera identification method provided by the invention comprises the following steps: 1) extracting the whole genome DNA of the sample to be detected; 2) using the whole genome DNA as a template, performing PCR amplification with the DNA barcode primer pair to obtain an amplified product 3) Sequencing the amplified product, obtaining the specific sequence information of the amplified product, comparing the specific sequence information with the standard sequence of the Liriodendron tulipifera DNA barcode, and determining the tree species and regional provenance of the sample to be tested, the The method is simple in operation and high in accuracy.
Owner:INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
Miticide for preventing and treating wheat stone mites of peach trees and preparation method of miticide
InactiveCN104430659AImprove insecticidal effectEasy to prepareBiocideAnimal repellantsJapanese barberryLiriodendron tulipifera
The invention discloses a miticide for preventing and treating wheat stone mites of peach trees and a preparation method of the miticide. The miticide comprises the following raw materials: a liriodendron tulipifera extract, a ginger extract, a daphne odora extract, a perilla extract, a ricinus communis extract and a berberis thunbergii extract. The miticide for preventing and treating wheat stone mites disclosed by the invention has the best effect, and a long pesticide effect; and the killing rate can be up to over 92%.
Owner:李晓枫
Method for separating therapeutic agent for chronic myelogenous leukemia from bark of liriodendron tulipifera l.
InactiveCN105283194AOrganic active ingredientsAntineoplastic agentsLiriodendron tulipiferaEthyl acetate
The present invention provides a method for separating and preparing a therapeutic agent for chronic myelogenous leukimeia capable of effectively inhibiting growth of T315I, which is known as a mutation of a BCR-ABL fusion gene that causes chronic myelogenous leukemia, comprising the steps of: 1) preliminary extracting by adding ethyl acetate to finely chopped bark of Liriodendron tulipifera L., adding butanol to an extracted fluid for layer-separating an ethyl acetate layer and a butanol layer, removing the butanol layer, and decompression-condensing the remainder to obtain a crude extract; and 2) adding a C1-C3 lower alcohol aqueous solution and n-hexane to the obtained crude extract, removing lipids and water insoluble material dissolved in the n-hexane layer, and obtaining and separating a lower alcohol aqueous solution layer to separate and refine highly pure epi-Tulipinolide and costunolide.
Owner:CHO DANG PHARM
Application of croton tiglium alkaloid in preparation of medicine for treating lung cancer
InactiveCN105194126AModerate conditionModerate roundnessOrganic active ingredientsPill deliveryCancer cellLiriodendron tulipifera
The invention discloses application of croton tiglium alkaloid in preparation of medicine for treating lung cancer, and belongs to the technical field of medicine. The medicine is prepared from, by weight, 5-10 parts of croton tiglium alkaloid, 1-2 parts of naringin, 2-4 parts of scutellaria baicalensis alkaloid, 0.5-2 parts of purple quercetin, 1-2 parts of liriodendron tulipifera alkaloid, 2-5 parts of coptidis rhizoma alkaloid, 1-3 parts of tacca plantaginea saponin and 2-6 parts of radix glycyrrhizae alkaloid. Croton tiglium alkaloid in the medicine restrains growth of lung adenocarcinoma cells A549 and promotes lung adenocarcinoma cells A549 to die through a Bax / Bcl-2 approach, medicine effects of all the raw materials of dropping pills supplement one another and bring out the best in one another, and the medicine has remarkable effects on restraining growth of lung cancer cells and promoting lung cancer cells to die under the mutual concurrent effects of all the raw materials, has an effect on treating lung cancer, is remarkable in curative effect, relieves pains of patients, prolongs lifetime, improves life quality, and provides a new direction for treatment of clinical lung cancer.
Owner:QINGDAO CENT HOSPITAL
Method for extracting treatment ingredients for gastrointestinal disease from bark of liriodendron tulipifera
The present invention relates to a method for extracting treatment ingredients for gastrointestinal diseases from the bark of Liriodendron tulipifera, the treatment ingredients containing epitulipinolide and costunolide as active ingredients, and to a therapeutic agent for gastrointestinal diseases, containing epitulipinolide and costunolide extracted using said method.
Owner:CHO DANG PHARM
A method for identifying the type of progeny tree species of Liriodendron tulipifera stand
ActiveCN108660250BGood repeatabilityImprove stabilityMicrobiological testing/measurementBiotechnologyLiriodendron
Owner:NANJING FORESTRY UNIV
Rapid propagation method of Liriodendron chinense (Hemsl.)Sarg. x Liriodendron tulipifera L. with bud as an explant
InactiveCN110073978AReproduce fastIncrease the multiplication factorPlant tissue cultureHorticulture methodsLiriodendron tulipiferaBud
The invention discloses a rapid propagation method of Liriodendron chinense (Hemsl.)Sarg. x Liriodendron tulipifera L. with a bud as an explant, which comprises the steps of pretreatment and disinfection of three genotypes of XY1, XY2 and T8, culture by a primary medium, a subculture medium, a strong seedling medium and a rooting medium, acclimatization and transplant and the like. The method of the invention is suitable for the rapid propagation of the Liriodendron chinense (Hemsl.)Sarg. x Liriodendron tulipifera L., and has the advantages of rapid propagation speed, large proliferation coefficient and high transplanting survival rate. Experiments confirm that the average starting rate of the highest primary culture of T8 genotype reaches 72% under the optimal culture conditions, the average proliferation coefficient of the maximum subculture of XY1 genotype reaches 3.70; the average single plant leaf number of the maximum strong seedling culture of XY2 genotype reaches 8.37, the average rooting rate of the highest rooting culture reaches 51.67%, and the final survival rate of the acclimatization and transplant is up to 85%.
Owner:NANJING FORESTRY UNIV
A kind of screening method of the SNP marker of Liriodendron tulipifera genome
ActiveCN108754005BQuality improvementValid statement accuracyMicrobiological testing/measurementLiriodendron tulipiferaGel electrophoresis
The invention discloses a method for screening the SNP markers of Liriodendron tulipifera genome. The F1 generation individuals obtained by crossing Liriodendron tulipifera 'NK' and Liriodendron tulipifera 'LS' are used as materials, and the reference genome of Liriodendron tulipifera obtained by sequencing is used. Based on this, use Primer5.0 software to design PCR amplification primers and test the amplification efficiency and specificity of the primers, select 27 candidate SNP sites for primer design, and use agarose gel electrophoresis to detect the screening results after PCR amplification , and through the verification of the provenance materials, the SNP markers of the Liriodendron tulipifera genome were finally obtained. The present invention selects 27 SNP sites from the genome database of Liriodendron tulipifera, synthesizes primers, and sends out sequencing after PCR amplification detection. The results show that all sites are real SNP sites, indicating the quality of SNP sites. Reliable, the standard used is good, and the high-quality SNP markers obtained can be used for follow-up work such as genetic linkage maps.
Owner:NANJING FORESTRY UNIV
Pharmaceutical composition containing as active ingredient extract from bark of liriodendron tulipifera
InactiveCN104125834AHigh dissolution rateImprove solubilityOrganic active ingredientsDigestive systemLiriodendron tulipiferaGastrointestinal disorder
The present invention relates to a pharmaceutical composition containing, as active ingredients, epitulipinolide and costunolide extracted from the bark of Liriodendron tulipifera. More specifically, the present invention relates to a pharmaceutical composition for treating the gastrointestinal tract, the composition containing, as active ingredients, epitulipinolide and costunolide, which are the active ingredients of the extract from the bark of Liriodendron tulipifera, and to the use thereof as a therapeutic agent for gastrointestinal diseases.
Owner:CHO DANG PHARM
Pharmaceutical composition containing liriodendron tulipifera l. extract for treating chronic myelogenous leukemia
InactiveUS20160081978A1Effective treatmentBiocideOrganic chemistryLiriodendron tulipiferaChronic myeloid leukaemia
The present invention relates to a pharmaceutical composition containing, as an active ingredient, a Liriodendron tulipifera L. bark extract for treating chronic myelogneous leukemia. More specifically, the present invention relates to the pharmaceutical composition, containing, as the active ingredient, the Liriodendron tulipifera L. bark extract useful in selectively inhibiting mutant enzyme T315I from a mutation of a BCR-ABL fusion gene that causes chronic myelogenous leukemia, and to a use of epi-Tulipinolide which is an active ingredient of the composition.
Owner:CHO DANG PHARM
Method for extracting treatment ingredients for gastrointestinal diseases from bark of liriodendron tulipifera
InactiveUS20150011624A1Preventing and treating gastritisPreventing and treating and stomach ulcerBiocideDigestive systemDiseaseLiriodendron tulipifera
The present invention relates to a method for extracting treatment ingredients for gastrointestinal diseases from the bark of Liriodendron tulipifera, the treatment ingredients containing epitulipinolide and costunolide as active ingredients, and to a therapeutic agent for gastrointestinal diseases, containing epitulipinolide and costunolide extracted using said method.
Owner:CHO DANG PHARM
A method for plant regeneration using deionized formamide for pure solid culture of somatic embryos of Liriodendron chinensis
ActiveCN106212287BImprove induction abilityEfficient and convenient accessHorticulture methodsPlant tissue cultureLiriodendron tulipiferaMaterial transfer
The invention discloses a somatic embryo all-solid cultivation plant regeneration method of hybridized liriodendron tulipifera by deionized formamide. The method includes the steps of: 1) inducing embryogenic callus of hybridized liriodendron tulipifera; 2) dark-culturing the embryogenic callus of the hybridized liriodendron tulipifera at 24 DEG C with a basic culture medium of 3 / 4MS + 5 mg / L of VC + 30 g / L of saccharose + 0.01-1 mg / L of the deionized formamide; 3) moving the material to a tissue culture bottle in a light culturing room, and continuously culturing the material with 3 / 4MS as a basic culture medium to obtain a regenerated plant; and 4) domesticating and transplanting the regenerated plant. Through the all-solid cultivation method, two somatic embryo, suspension cultivation and plate cultivation, during a somatic embryo generation process are omitted, thereby optimizing and enriching the somatic embryo regeneration system of the hybridized liriodendron tulipifera and further more high-effectively and conveniently obtaining high-quality somatic embryo regeneration regenerated plants. The method can establish the all-solid culture medium somatic embryo generation system, which is stable and high-frequent, is simple in operation and has excellent nursery stock resistance, of the hybridized liriodendron tulipifera, and provides references for establishing a more high-effective genetic transformation receptor system of the hybridized liriodendron tulipifera.
Owner:NANJING FORESTRY UNIV
A kind of gene of transcription factor lcbhlh52 of Liriodendron tulipifera and its application
ActiveCN112746079BGrowth inhibitionImprove tolerancePlant peptidesFermentationBiotechnologyLiriodendron tulipifera
The invention discloses a Liriodendron tulipifera transcription factor LcbHLH52 gene and an application thereof, belonging to the technical field of plant genetic engineering. The present invention analyzes the bHLH family sequence of Liriodendron tulipifera through bioinformatics tools, and then successfully clones the LcbHLH52 transcription factor gene through sequencing and homologous comparison. Construct an overexpression vector for the cloned LcbHLH52 transcription factor gene, transform Arabidopsis thaliana, and obtain T1 generation seeds; put LcbHLH52 transgenic Arabidopsis thaliana T2 generation plants and wild-type Arabidopsis plants into 4°C low temperature treatment for 3 days at the same time, and the phenotype It was observed that the growth of transgenic plants was less affected by low temperature stress, while the growth of wild-type plants was inhibited, and the leaves had wilting phenomenon. The results show that the LcbHLH52 gene of Liriodendron thaliana can enhance the tolerance of Arabidopsis plants to low temperature stress, and has important application value in molecular breeding for improving low temperature stress of plants.
Owner:NANJING FORESTRY UNIV
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