30 results about "Liriodendron tulipifera" patented technology

The invention discloses a liriodendron tulipifera cutting propagation method. The liriodendron tulipifera cutting propagation method comprises the following steps that healthy and strong trees free of diseases and insect pests are selected and 1-2a thick and solid branches are sheared, wherein the length of shoots ranges from 13 cm to 16 cm, and each shoot has 2-3 buds; cuts of cuttings are flat, the distance from each upper cut to a nearest bud is 3 cm, and the distance from each lower cut to a nearest bud is 0.5-1.0 m; the upper physiological ends of bundled cutting shoots are upwards soaked in flowing water before cuttage, the upper cuts are not submerged in water, and the soaking time is 5-7 days; therefore, rooting can be promoted, spouting is advanced, and the growing amount of nursery stocks can be further increased; the cutting shoots are exposed out of the ground for 1-2 cm when the cuttings are buried in soil in a straight cutting mode, and one-bud exposure is preferable; the planting row space is 20 cm*30 cm, watering is performed after cuttage, and the cuttings are covered by using plastic films. By means of the liriodendron tulipifera cutting propagation method, cutting rot can be prevented, cutting rooting and propagation can be further accelerated, and the survival rate of propagated liriodendron tulipifera cuttings is greatly improved.

The invention discloses a method for acquiring a two-dimensional electrophoretogram of nectar protein of liriodendron tulipifera by the aid of a two-dimensional electrophoresis system. After a nectar sample is filtered by an ultramicro filter membrane, the nectar protein is concentrated and purified by a protein ultrafiltration and concentration purification column, the concentration of the purified and concentrated nectar protein is detected with a Bradford method, the molecular weight distribution range of the nectar protein of the liriodendron tulipifera is pre-detected through one-dimensional lauryl sodium sulfate polyacrylamide gel electrophoresis, finally, proper standard protein and a gel strip with a proper length and pH range are selected according to a one-dimensional electrophoresis result, the nectar protein of the liriodendron tulipifera is separated with the two-dimensional electrophoresis system, and the two-dimensional electrophoretogram is acquired. The method has the characteristics that the operation is simple, the protein extraction rate is high and few interfering substances exist and is suitable for special materials such as liquid samples, the high-quality two-dimensional gel electrophoretogram with high resolution ratio, clear protein points, larger numbers, uniform distribution and clear background can be obtained, and the experiment repeatability and the stability are good.

The present invention relates to a pharmaceutical composition containing, as active ingredients, epitulipinolide and costunolide extracted from the bark of Liriodendron tulipifera. More specifically, the present invention relates to a pharmaceutical composition for treating the gastrointestinal tract, the composition containing, as active ingredients, epitulipinolide and costunolide, which are the active ingredients of the extract from the bark of Liriodendron tulipifera, and to the use thereof as a therapeutic agent for gastrointestinal diseases.

The invention discloses a traditional Chinese medicament for treating wind-phlegm in lung type asthma. The medicament is characterized by being prepared from the following medicinal materials in parts by weight: 10 parts of lepidium sativum, 10 parts of immature trifoliate-orange fruit, 6 parts of liquorice, 20 parts of dried ginger, 25 parts of liriodendron tulipifera, 15 parts of betel, 20 parts of lloydia thibetica baker, 5 parts of asarum, 10 parts of tibetan berneuxia, 15 parts of codonopsis pilosula, 40 parts of yam, 10 parts of semen raphani and 8 parts of lan tree flower. The medicinal materials are compatible and synergetic by scientific formula and proper proportion, and the prepared traditional Chinese medicament has the effects of dispelling wind and flushing phlegm, depressing qi and relieving asthma, and can be used for effectively treating wind-phlegm in lung type asthma. Five years of application on 120 patients indicates that the effective rate of the traditional Chinese medicament is 96 percent, and relapse is prevented after healing.

The invention discloses an identification method for the types of descendant tree species of Liriodendron forest and a special kit thereof. The method comprises the following steps: 1) extracting leafDNA (Deoxyribonucleic Acid) of the descendant tree species; 2) performing SSR-PCR (Simple Sequence Repeat-Polymerase Chain Reaction) detection; 3) determining the descendant tree species, wherein thedescendant tree species which can specifically amplify a product of 112bp by electrophoresis are Liriodendron chinense (Hemsl.) Sarg., the descendant tree species which can specifically amplify a product of 124bp by electrophoresis are Liriodendron tulipifera Linn., and the descendant tree species which can specifically amplify products of 112bp and 124bp are hybrid of Liriodendron chinense and Liriodendron tulipifera. The identification method for the types of the descendant tree species of Liriodendron forests disclosed by the invention has the advantages of high repeatability and high stability, can effectively identify the types of descendant tree species of Liriodendron natural forests and artificial forests, particularly the descendant trees of Liriodendron natural forests and artificial forests at a seedling stage, and can identify whether seeds of seed orchards are Liriodendron chinense (Hemsl.). seeds, Liriodendron tulipifera seeds or seeds of hybrid of Liriodendron chinenseand Liriodendron tulipifera. The identification method has the advantages of fastness, high efficiency, accurate result and very high practicality.

The invention discloses a method for screening SNP markers of a Liriodendron genome. F1-generation individuals obtained from Liriodendron tulipifera 'NK' and Liriodendron 'LS' by hybridization are taken as a material, a Liriodendron reference genome obtained by sequencing is taken as the basis, PCR amplification primers are designed by Primer 5.0 software, amplification efficiency and specificityof the primers are verified, 27 candidate SNP loci are selected for primer design, a screening result is detected by agarose gel electrophoresis after PCR amplification, provenance material are verified, and finally, the SNP markers of the Liriodendron genome are obtained. 27 SNP loci are selected from a Liriodendron genome database for primer synthesis, products are subjected to PCR amplificationdetection and sent out for sequencing, a result shows that all the loci are real SNP loci, which means that the SNP loci are reliable in quality, used standards are better, and the obtained high-quality SNP markers can be applied to follow-up work such as genetic linkage maps and the like.

The invention discloses a liriodendron transcription factor LcbHLH16421 gene and application thereof, and belongs to the technical field of plant genetic engineering. The liriodendron tulipifera bHLH family sequence is analyzed through a bioinformatics tool, and then the LcbHLH16421 transcription factor gene is obtained through cloning through sequencing and homologous comparison. An overexpression vector for the LcbHLH16421 gene is constructed, and arabidopsis thaliana is converted to obtain T1-generation seeds; LcbHLH16421 transgenic arabidopsis thaliana T2-generation plants and wild plants are treated at the low temperature of 4 DEG C for 3 days at the same time, phenotypic observation shows that the influence of low-temperature stress on the growth of the transgenic plants is small, the growth of the wild plants is inhibited, and leaves are withered. The result shows that the liriodendron tulipifera LcbHLH16421 gene can enhance the tolerance of an arabidopsis thaliana plant to low-temperature stress, and has important application value in molecular breeding for improving the low-temperature stress of the plant.

The invention discloses a liriodendron transcription factor LcbHLH02399 gene as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The bHLH family sequence of liriodendron is analyzed through a bioinformatics tool, and then the LcbHLH02399 gene for coding the bHLH transcription factor is cloned through sequencing and homologous comparison. The method comprises the following steps: constructing an overexpression vector for an LcbHLH02399 gene, transforming hybrid liriodendron calluses, and carrying out liquid culture to obtain transgenic hybrid liriodendron; compared with phenotypes of LcbHLH02399 transgenic hybrid liriodendron plants and wild plants which are subjected to low-temperature treatment in an illumination incubator at 4 DEG C for 3 days, the influence of low-temperature stress on the growth of the transgenic plants is small, the growth of the wild plants is inhibited, and leaves are withered. The result shows that the liriodendron tulipifera LcbHLH02399 gene can enhance the tolerance of a liriodendron tulipifera plant to low-temperature stress, and has an important application value in molecular breeding for improving the low-temperature stress of the plant.

The invention discloses a miticide for preventing and treating wheat stone mites of peach trees and a preparation method of the miticide. The miticide comprises the following raw materials: a liriodendron tulipifera extract, a ginger extract, a daphne odora extract, a perilla extract, a ricinus communis extract and a berberis thunbergii extract. The miticide for preventing and treating wheat stone mites disclosed by the invention has the best effect, and a long pesticide effect; and the killing rate can be up to over 92%.

The invention discloses application of croton tiglium alkaloid in preparation of medicine for treating lung cancer, and belongs to the technical field of medicine. The medicine is prepared from, by weight, 5-10 parts of croton tiglium alkaloid, 1-2 parts of naringin, 2-4 parts of scutellaria baicalensis alkaloid, 0.5-2 parts of purple quercetin, 1-2 parts of liriodendron tulipifera alkaloid, 2-5 parts of coptidis rhizoma alkaloid, 1-3 parts of tacca plantaginea saponin and 2-6 parts of radix glycyrrhizae alkaloid. Croton tiglium alkaloid in the medicine restrains growth of lung adenocarcinoma cells A549 and promotes lung adenocarcinoma cells A549 to die through a Bax/Bcl-2 approach, medicine effects of all the raw materials of dropping pills supplement one another and bring out the best in one another, and the medicine has remarkable effects on restraining growth of lung cancer cells and promoting lung cancer cells to die under the mutual concurrent effects of all the raw materials, has an effect on treating lung cancer, is remarkable in curative effect, relieves pains of patients, prolongs lifetime, improves life quality, and provides a new direction for treatment of clinical lung cancer.

The present invention relates to a pharmaceutical composition containing, as an active ingredient, a Liriodendron tulipifera L. bark extract for treating chronic myelogneous leukemia. More specifically, the present invention relates to the pharmaceutical composition, containing, as the active ingredient, the Liriodendron tulipifera L. bark extract useful in selectively inhibiting mutant enzyme T315I from a mutation of a BCR-ABL fusion gene that causes chronic myelogenous leukemia, and to a use of epi-Tulipinolide which is an active ingredient of the composition.

The present invention relates to a method for extracting treatment ingredients for gastrointestinal diseases from the bark of Liriodendron tulipifera, the treatment ingredients containing epitulipinolide and costunolide as active ingredients, and to a therapeutic agent for gastrointestinal diseases, containing epitulipinolide and costunolide extracted using said method.