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A Medium for Promoting Somatic Embryogenesis of Liriodendron chinensis by Using γ-Aminobutyric Acid and Its Application

A technology for hybridizing Liriodendron tulipifera and Liriodendron tulipifera body is applied in the directions of application, plant regeneration, horticulture, etc., and achieves the effects of large number, improved efficiency and quality, and high quality.

Active Publication Date: 2022-03-01
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, GABA has been proved to play a certain coordinating role between plant secondary growth and stress adaptation, but its role in promoting cell growth and differentiation and inducing somatic embryogenesis under the conditions of plant tissue or cell culture in vitro has rarely been reported.

Method used

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  • A Medium for Promoting Somatic Embryogenesis of Liriodendron chinensis by Using γ-Aminobutyric Acid and Its Application
  • A Medium for Promoting Somatic Embryogenesis of Liriodendron chinensis by Using γ-Aminobutyric Acid and Its Application
  • A Medium for Promoting Somatic Embryogenesis of Liriodendron chinensis by Using γ-Aminobutyric Acid and Its Application

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Experimental program
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Effect test

Embodiment 1

[0029] 1) Body embryonic induction medium:

[0030] The basic medium was added to 3 / 4 ms + Vc 5 mg / L + sucrose 30 g / L, respectively, respectively, with different concentrations of gamma-aminobutyric acid, constituting hybrid goosebedular embryos induced medium. In the control of the basic medium without γ-aminobutyric acid, 6 concentration gradients (0 mg / l, 2 mg / L, 4 mg / L, 6 mg / L, 8 mg / L, 10 mg / L) were at least each concentration. Turning a three-dish, 5 cells were vaccinated, each of which was about 0.5 cm per capita. 24 ° C dark culture, each 25 days.

[0031] Morphological observation of embryogenic callus induced different time points, and statistics on the body embryos in each development period in the body embryo induction 90D, as the total emborexia, has been developed as a torpedo and sub-bladed body embryo Mature body embryo. After the total embryos, the total embryo is scaling, and the surface is smoothly separated, the surface is smoothly separated, and...

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Abstract

The invention discloses a medium for promoting somatic embryogenesis of Liriodendron chinensis by using γ-aminobutyric acid and its application, belonging to the technical field of plant tissue culture. The culture medium for somatic embryogenesis of hybrid Liriodendron chinensis of the present invention is based on 3 / 4MS+VC 5mg / L+sucrose 30g / L as the base medium, and γ-aminobutyric acid of 0~10mg / L is added; the added γ ‑GABA can promote the generation of somatic embryos in the callus of Liriodendron chinensis, accelerate the development of somatic embryos, and improve the efficiency of somatic embryogenesis. The culture medium for somatic embryogenesis of Liriodendron chinensis provided by the present invention is applied in somatic embryogenesis and tissue culture of Liriodendron chinensis, the number of somatic embryogenesis is large, the speed is fast, and the quality is high, and the number of somatic embryos induced is very high. Significantly higher than that of the control group, the number of lateral roots and root length were significantly higher than that of the control group, and an average of 20 regenerated plants can be formed per callus, which has high practical application value.

Description

Technical field [0001] The present invention belongs to the field of plant composition culture, and in particular, the present invention relates to the medium and its application of the bumper surface of hybrid goosebuta. Background technique [0002] Hybrid Wingron Sino-Americanum is beautiful, rapid growth, is widely used in garden greening and fine wood processing. Nanjing Forestry University established a stable and efficient hybrid mesenchymal embryos and a rapid forming system (Chen Jinhui et al, 2003), and achieved scale. Chen Jinhui, etc. In addition, the hormone changes in the process of hybrid goosebus have also achieved significant progress in the expression of related genes and changes in protein changes. At present, some problems still need to improve, such as shortening the body embryo induced cycle, improving the induction efficiency of the body embryo, reducing the amount of malformed body embryos. [0003] γ-Aminobuturic Acid, Gaba is a tetra-carbon non-protein a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈金慧陈婷婷施季森陆叶王丹丹陈新颖翁禹豪成铁龙
Owner NANJING FORESTRY UNIV
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