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Method for promoting rapid development of gametophytes of chelmydomonas palustris

A technology of gametophyte and Cheschiella, which is applied in the field of promoting the rapid development of Chessia pachyphyllum gametophytes, can solve the problems of reduced yield at harvest time, asynchronous development of male and female, and long development time, so as to reduce the time required for seedling cultivation and promote synchronization Growth, the effect of accelerating the development process

Active Publication Date: 2022-05-13
SHANDONG ORIENTAL OCEAN SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the process of cultivating seedlings using gametophytes, it was found that if the common method of kelp gametophyte seedlings was adopted, the gametophytes of Chrysophylla pachyphyllum had the disadvantages of asynchronous development of male and female, too long development time, and low conversion rate of sporophytes, resulting in low utilization of gametophytes , Long seedling cycle and late seedling out of the warehouse not only increase the cost of seedling raising, but also shorten the suitable growth time in the sea area, and ultimately reduce the yield during harvest

Method used

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  • Method for promoting rapid development of gametophytes of chelmydomonas palustris
  • Method for promoting rapid development of gametophytes of chelmydomonas palustris
  • Method for promoting rapid development of gametophytes of chelmydomonas palustris

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1: optimize nitrogen source

[0023] Take an appropriate amount of the female and male gametophytes of Chrysophylla pachyphyllum, break them up for 5 minutes with a micro-submersible pump, and then filter them with a 300-mesh sieve to obtain a gametophyte cell segment with 2 to 5 cells as the main part. The female and male gametophytes were mixed at a mass ratio of 2:1, cultured in a Petri dish, at a temperature of 10°C, under white light, and an illumination intensity of 30 μmol photons / (m 2 s), photoperiod 20L: 4D, culture medium added effective N10mg / L, KH 2 PO 4 -P 1mg / L and FeC 6 h 5 o 7 - Sterilized seawater with Fe 0.5mg / L (sterilized by boiling to reduce the pollution of diatoms, etc.), and NaNO as nitrogen source 3 , CO(NH 2 ) 2 , NH 4 NO 3 , NH 4 Cl and NH 4 HCO 3 During the cultivation process, the culture medium was changed every 3 days. At the same time, the growth and development of the gametophytes were observed under the microscope...

Embodiment 2

[0030] Example 2: Optimizing Light Quality

[0031] Take an appropriate amount of female and male gametophytes of Chrysophylla pachyphyllum, break them up for 5 minutes with a micro-submersible pump, and then filter them with a 300-mesh sieve to obtain gametophytic cell segments with 2 to 5 cells as the main part. , and male gametophytes were mixed at a mass ratio of 2:1 and cultured in a petri dish at a temperature of 10°C and a light intensity of 30 μmol photons / (m 2 s), photoperiod 20L: 4D, the culture medium is added effective NaNO 3 -N 10mg / L, KH 2 PO 4 -P1mg / L and FeC 6 h 5 o 7- Fe 0.5mg / L sterilized seawater (sterilized by boiling), using different wavelengths of visible light (white light, blue light, red light), changing the culture medium every 3 days during the culture process, and at the same time under the microscope Observe the growth and development status of gametophytes, observe and take pictures, randomly observe 10 100×fields of view in each petri dish...

Embodiment 3

[0037] Example 3: Optimizing Fe Concentration

[0038] Take an appropriate amount of the female and male gametophytes of Chrysophylla pachyphyllum, break them up for 5 minutes with a micro-submersible pump, and then filter them with a 300-mesh sieve to obtain a gametophyte cell segment with 2 to 5 cells as the main part. Female and male gametophytes were mixed at a mass ratio of 2:1 and cultured in a Petri dish at a temperature of 10°C under white light with a light intensity of 30 μmol photons / (m 2 s), photoperiod 20L: 4D, the culture medium is added effective NaNO 3 -N10mg / L, KH 2 PO 4 -P1mg / L and FeC 6 h 5 o 7 - Fe sterilized seawater (sterilized by boiling), the amount of Fe added is set to 5 gradients, respectively 0.05mg / L, 0.1mg / L, 0.5mg / L, 1mg / L, 2mg / L, each There were 3 replicates in each group, and the culture medium was changed every 3 days during the culture process. At the same time, the growth and development of gametophytes were observed under a microscope...

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Abstract

The invention discloses a method for promoting rapid development of gametophytes of Czostera crassipes, and particularly relates to a method for promoting rapid development of gametophytes of Czostera crassipes by adopting blue light as a light source, adopting CO (NH2) 2 as a nitrogen source and adding 0.5 mg / L of FeC6H5O7-Fe into a culture solution in the development stage of the gametophytes, and adopting the optimal development conditions of the gametophytes of Czostera crassipes under other conditions. The method provided by the invention can effectively accelerate the development process of the gametophytes of the chlamydomonas palustris, promote the synchronous development of the male and female gametophytes and improve the sporophyte conversion rate, thereby improving the utilization rate of the gametophytes, reducing the time required for seedling culture, improving the stock-out specification of seedlings and reducing the cost of seedling breeding.

Description

technical field [0001] The invention relates to a method for promoting the rapid development of the gametocytes of Chrysalis pachyphyllum, belonging to the technical field of seawater culture. Background technique [0002] Saccharina sculpera, also known as Saccharina sculpera and Laminaria kelp, belongs to Phaephyta (Phaephyta), Laminariales (Laminariales), Laminariaceae (Laminariaceae), is a large brown algae rich in fucoidan . Fucoidan is a kind of polysaccharide containing L-fucose and sulfuric acid groups. It has various biological functions such as anticoagulant, antitumor, antioxidation and enhancing the immune function of the body, so it is widely used in the medical field and modern food industry. The content of fucoidan in Chrysophylla pachyphyllum is about three times that of kelp, and it has great development and application value as a raw material for extracting fucoidan. Due to the late natural maturation time of sporophytes, it is difficult to match the tim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/005
Inventor 陈书秀王伟伟孙娟史良赵楠李霞李晓捷孙建晓
Owner SHANDONG ORIENTAL OCEAN SCI TECH
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