2 results about "Daphne odora" patented technology

The invention discloses a preparation technology of yak manure and plant fiber composite Tibetan paper, and relates to the technical field of composite Tibetan paper preparation, and the preparation technology comprises the following steps: screening dried yak manure, removing pebbles and hair impurities, soaking in clear water, and scrubbing to obtain a yak manure material; the yak manure material is placed in a high-temperature steam environment of 100 DEG C or above for continuous sterilization for 30 min, and then mechanical crushing treatment is performed to obtain short-fiber-shaped yak manure crude fiber pulp; the method comprises the following steps: cleaning radix euphorbiae lantu roots and / or branches and barks of daphne odora, and extracting plant long fibers by adopting a mode of combining soaking, beating and tearing, or cooking and treating the radix euphorbiae lantu roots or the branches and barks of daphne odora by adopting a mild alkaline method to obtain traditional plant long fiber pulp; the short-fiber-shaped yak manure crude fiber pulp and the traditional plant long fiber pulp are put into a pulp mixing pool according to the mass ratio of 40%-60%: 30%-50%, the mass ratio of the yak manure crude fiber pulp is preferably 70%-90%, and the aim that the raw materials basically come from the Qinghai-Tibet Plateau is achieved.

This invention relates to the field of plant biotechnology, specifically a two-stage tissue culture regeneration method for Daphne odora based on sugar source regulation. This invention constructs a two-stage tissue culture regeneration system for Daphne odora using both sugar-containing and sugar-free methods. Through six coordinated steps—explant pretreatment, stepwise disinfection, callus induction, adventitious bud differentiation, sugar-free rooting enhancement, and hardening-off transplanting—it solves the technical problems of low germination rate in traditional Daphne odora propagation and poor survival rate after conventional tissue culture transplanting. It achieves stable induction, differentiation, and transplant survival of Daphne odora tissue culture seedlings, shortens the tissue culture propagation cycle, and ensures proper preservation of Daphne odora germplasm resources. This provides stable and feasible technical support for large-scale seedling production, germplasm resource protection, and the industrialized production of Daphne odora medicinal materials.