A kind of screening method of the SNP marker of Liriodendron tulipifera genome

A screening method, the technology of Liriodendron tulipifera, applied in the field of genome, can solve the problem of lack of molecular markers, etc., and achieve the effect of reliable quality

Active Publication Date: 2019-08-20
NANJING FORESTRY UNIV
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Problems solved by technology

[0003] Since the 1940s, the origin, distribution, phylogenetic status, asexual reproduction, flowering and fruiting habit, and hybrid breeding of Liriodendron have been studied at home and abroad, and molecular markers such as RAPD and SSR have been developed. There are still problems such as the lack of molecular markers suitable for the genetics research of Liriodendron, so the development of SNP markers with high polymorphism will help the investigation and evaluation of genetic resources of Liriodendron plants and the research of hybrid breeding

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  • A kind of screening method of the SNP marker of Liriodendron tulipifera genome
  • A kind of screening method of the SNP marker of Liriodendron tulipifera genome
  • A kind of screening method of the SNP marker of Liriodendron tulipifera genome

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Embodiment 1

[0026] A screening method for the SNP marker of Liriodendron tulipifera genome, the specific steps are as follows:

[0027] 1. Materials

[0028] The mating combination of hybrid parents is Liriodendron tulipifera ‘NK’ × Liriodendron chinense ‘LS’, referred to as ‘NK’בLS’, with Liriodendron tulipifera ‘NK’ as the female parent and Liriodendron chinense ‘LS’ as the male parent. The female parent 'NK' is a good provenance in the eastern coast of North America, and the male parent 'LS1' is a provenance in the southeastern inland of China. At the end of April and the beginning of May 2011, the Xiashu Forest Farm of Nanjing Forestry University carried out artificial cross-pollination of Liriodendron tulipifera. 2-3 days after the completion of pollination, when the ovary expands, remove the paper bag. After the aggregated fruit develops and matures in about 140-160 days, harvest it manually. After it is naturally air-dried, remove the seeds of the wing and place it in a cold stor...

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Abstract

The invention discloses a method for screening the SNP markers of Liriodendron tulipifera genome. The F1 generation individuals obtained by crossing Liriodendron tulipifera 'NK' and Liriodendron tulipifera 'LS' are used as materials, and the reference genome of Liriodendron tulipifera obtained by sequencing is used. Based on this, use Primer5.0 software to design PCR amplification primers and test the amplification efficiency and specificity of the primers, select 27 candidate SNP sites for primer design, and use agarose gel electrophoresis to detect the screening results after PCR amplification , and through the verification of the provenance materials, the SNP markers of the Liriodendron tulipifera genome were finally obtained. The present invention selects 27 SNP sites from the genome database of Liriodendron tulipifera, synthesizes primers, and sends out sequencing after PCR amplification detection. The results show that all sites are real SNP sites, indicating the quality of SNP sites. Reliable, the standard used is good, and the high-quality SNP markers obtained can be used for follow-up work such as genetic linkage maps.

Description

technical field [0001] The invention belongs to the technical field of genomes, and in particular relates to a screening method for SNP markers of Liriodendron tulipifera genome. Background technique [0002] Liriodendron belongs to the genus Liriodendron in the family Magnoliaceae. Today there are only two species, namely Chinese tulipwood (L.chinese Sarg.) (also known as mandarin tree) and North American tulipifera (L.tulipifera L.). Liriodendron has grown in northern Europe, Greenland, and Alaska at higher latitudes in the northern hemisphere in the late Jurassic of the Mesozoic; it was widely distributed in Eurasia and North America in the Tertiary of the Cenozoic; after the glaciers of the Quaternary It is only distributed in the south of my country and the southeast of North America, becoming a typical relict plant. Liriodendron tulipifera and Liriodendron tulipifera have a certain geographical isolation, but their interspecific compatibility is high (Wang Zhangrong, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6869
Inventor 施季森陆叶陈金慧龙晓飞杨立明肖保荣刘阳陈新颖张稼霁
Owner NANJING FORESTRY UNIV
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