28 results about "Electrophoretogram" patented technology

The invention discloses a device and method for automatically shearing and recycling agarose gel, and relates to the technical field of agarose gel cutting, and the device and method for automatically shearing and recycling agarose gel comprise an image acquisition unit, a three-axis moving mechanism, a gel cutting device, an electrophoresis apparatus and a control unit; the image acquisition unit comprises a light source assembly and an image acquisition camera corresponding to an irradiation area, the image acquisition camera is used for shooting a dyed electrophoresis image, and the light source assembly is used for obliquely irradiating gel to enable a sample strip to develop color; the control unit comprises an analysis processing unit; the analysis processing unit comprises a deep learning detection module and a data processing module; the control unit cooperates with the image acquisition unit, the three-axis moving mechanism and the gel cutting device, so that a complete automatic process from strip identification and positioning after electrophoresis to gel block cutting, grabbing, releasing and recycling is constructed.

The invention provides a primer group for identifying coilia ectenes, coilia brachygnathus and hybrids thereof, a detection method, a kit and application, and belongs to the technical field of molecular marker identification. In order to overcome the defects that morphological differences of coilia ectenes, coilia brachygnathus and hybrid adults are not obvious and the like, a primer pair A or a primer pair B is designed for gene amplification, and the coilia ectenes, the coilia brachygnathus and the hybrid adults are identified by comparing sequence differences after amplification. The sequences of amplification products of the coilia ectenes, the coilia brachygnathus and the hybrids have obvious differences, the types of the amplification products can be classified through molecular markers, and the reaction result is directly visualized in the form of a gel electrophoretogram without a sequencing means. The method is simple to operate, intuitive in result, low in requirement on experimental equipment and low in cost, and all work can be completed within several hours without statistics. According to the invention, the hybrid can be identified through a sequencing peak map, and compared with an SNP site identification type, the difference is more obvious, the statistical frequency is not needed, and the accuracy is high.

The invention relates to the field of bioinformatics and molecular biology, and discloses a gene single nucleotide polymorphism (SNP) site analysis method based on SNaPshot, which comprises the following steps: acquiring genome DNA sequence data of a sample to be detected, extracting SNP site information in the genome DNA sequence data, designing site specific primers, carrying out PCR amplification by using an SNaPshot technology, and determining the SNP site of the sample to be detected. And an electrophoretogram is obtained through capillary electrophoresis separation. By analyzing the maps, the genotype of each SNP site can be determined, and an SNP feature map can be constructed. And finally, performing statistical analysis based on the feature maps to obtain a target analysis result, evaluating gene polymorphism according to the target analysis result, and obtaining related information. According to the method, the efficiency and accuracy of SNP analysis can be improved, and support is provided for genetic research and individualized medical treatment.

The invention relates to the field of antibodies, in particular to an anti-troponin I monoclonal antibody and application thereof, performance analysis of a purified electrophoretogram of the antibody and a kit coupled with a monoclonal antibody HK401 coated magnetic particle component shows that the monoclonal antibody has excellent performance in cTni detection, and the cTni detection sensitivity is high. The method can be used as an object for further production and optimization.

The invention discloses a biological strip recognition system and method for Marker strip labeling. The method comprises the steps that firstly, an electrophoretogram comprising at least two Marker strips is obtained according to a collected gel electrophoretogram; then, a preset strip segmentation model is applied to segment the image area of each Marker strip in the electrophoretogram to obtain a strip segmentation image; inputting the strip segmentation image into a preset strip identification neural network and obtaining a strip identification graph; and finally, marking the Marker strip of which the strip type is a true strip in the strip identification graph to obtain and output a strip marking graph. Due to the fact that the segmentation model and the recognition neural network are used for sequentially conducting strip segmentation and pseudo-strip recognition on the gel electrophoretogram and then marking the Marker strip fragment values, the Marker strip marking efficiency and precision are greatly improved.

The invention relates to an identification method of pea seeds. The identification method comprises the following steps: constructing an electrophoretogram of esterase isoenzyme of to-be-detected pea seeds; judging the variety of the pea seeds to be detected by utilizing a characteristic spectrum band in an electrophoretogram of esterase isoenzyme of the known pea seeds; the known pea seeds are one or more of white peas, green peas, spotted peas, Canada No.1 and spotted peas G2682, and in an electrophoretogram, the white peas have characteristic bands P1, P2, P4 and P5, the green peas have characteristic bands P1, P2, P3 and P4, the spotted peas have characteristic bands P2 and P4, the Canada No.1 has characteristic bands P1, P2, P3 and P4, and the spotted peas G2682 has characteristic bands P1, P2 and P4; the P3 characteristic spectrum band of the Canada No.1 is higher than the P3 characteristic spectrum band of the green peas in strength. According to the method, the pea seeds can be quickly and accurately identified on the basis of characteristic bands in electrophoretograms of various pea esterase isoenzymes.

The invention discloses a large fragment deletion-based specific molecular marker for sex of spinibarbus hollandi and application of the specific molecular marker. According to the marker, whole genome re-sequencing data of male and female individuals of barbus hollandi is compared to a reference genome, and a female specific large fragment deletion region located on a No.43 chromosome (chr43) is screened out. Specific primers are designed according to the deletion region, and rapid and accurate identification of the gender of the spinibarbus hollandi can be realized through PCR amplification and electrophoretogram analysis. The method is suitable for sex identification of spinibarbus hollandi offspring seeds in the early development stage, and provides technical support for research on unisexual breeding, sex control and sex determination mechanism.

The invention provides a high-flux monosaccharide separation and quantification method and kit based on gel electrophoresis. The separation and quantification method comprises the following steps: (1) providing a fluorescence-labeled monosaccharide sample to be detected; (2) injecting the monosaccharide sample to be detected into a capillary electrophoresis system, and performing first electrophoretic separation under a first separation system to obtain a first electrophoretogram; the first separation system comprises borate-free first separation gel and a first buffer solution; (3) cleaning a capillary tube of the capillary electrophoresis system, injecting the monosaccharide sample to be detected into the capillary tube again, and performing electrophoretic separation for the second time in a second separation system to obtain a second electrophoretogram; the second separation system comprises second separation gel containing borate and a second buffer solution; and (4) carrying out qualitative identification and quantitative calculation on the mixed monosaccharide according to the migration time difference and peak overlapping condition of each monosaccharide component in the first electrophoretogram and the second electrophoretogram.

The invention discloses a W specific gene, a specific primer pair, a kit and a detection method for identifying male and female of Asiatic corn borer in various insect states. And the nucleotide sequence of the specific gene of W is shown as SEQ ID NO: 1. The invention also provides a specific primer pair for detecting the specific gene of W. The invention also provides a PCR detection method for rapidly identifying male and female of the Asiatic corn borer in each insect state, which comprises the following steps: extracting gDNA of the Asiatic corn borer, carrying out PCR amplification, and carrying out agarose gel electrophoresis on a PCR amplification product; the clone fragment of the W specific gene of the female worm has a corresponding band in an agarose gel electrophoretogram, and the male worm has no corresponding band. The method is suitable for male and female identification of Asiatic corn borers in each growth and development stage, and is short in detection time, simple to operate, low in cost and suitable for large-scale detection. The method is high in accuracy, and non-insect taxonomy professionals can identify male and female insect bodies of Asiatic corn borers in various growth and development stages through the method.

The application discloses an in-vitro detection method and kit for O-GlcNAc glycosylation modification of alpha-synuclein, and relates to the field of biochemical detection. Y289L The method comprises the following steps: extracting protein in a sample to be detected, adding UDP-GalNAz substrate beta4GalT Y289L The mutant enzyme and the reaction are carried out to obtain a protein system; DBCO-mPEG is added to the protein system, and the reaction product is subjected to electrophoretic separation; and according to an electrophoretogram, the proportion of the protein subjected to glycosylation modification and the molecular weight distribution thereof are calculated. The application marks specific molecular weight "tags" for O-GlcNAc glycosylation proteins, and the glycosylation-modified proteins are recognized by alpha-synuclein specific antibodies to show specific molecular weight sizes; and the alpha-synuclein polymerization states, such as dimers and polymers, subjected to O-GlcNAc glycosylation modification can be determined and quantified according to protein standards.

The invention provides a rare immune fixation electrophoretogram identification system based on a two-stage classification normal form, which is not limited to classification of common disease types, but adds identification of rare disease types on the premise of ensuring common disease type identification precision, so that the identification accuracy of the common disease types is improved. The vacancy of an existing immunofixation electrophoretogram recognition method based on deep learning in the aspect of rare disease type recognition is made up, and universality and practicability are greatly enhanced; meanwhile, a double-stage classification normal form method is adopted, so that the class imbalance ratio of the data set is effectively improved, and the influence caused by class imbalance of the data set is reduced; finally, a loss function LMF specially used for solving data class imbalance in the medical field is adopted, the recognition precision of a few classes is improved on the premise that the recognition precision of most classes is guaranteed, and then the overall recognition precision is improved.

The application discloses a method for detecting coumarin, comprising the following steps: (1) preparing the following solutions: borax aqueous solution, NaOH aqueous solution, boric acid aqueous solution, 2,6-dichloroquinone-4-chloroimine ethanol solution; (2) adjusting the pH value of the borax aqueous solution to 11.8-12.2 by using the NaOH aqueous solution to obtain a reaction buffer solution; (3) mixing the reaction buffer solution and a sample solution to be detected uniformly at a volume ratio of 2:1, and reacting under the condition of water bath heating; (4) adjusting the pH value of the solution to 8.6-9.0 by using the boric acid aqueous solution and pure water, and adding the 2,6-dichloroquinone-4-chloroimine ethanol solution, shaking uniformly, and then placing at room temperature for color development to obtain a sample solution to be detected; (5) detecting the sample solution to be detected by using the above capillary electrophoresis instrument, obtaining a sample capillary electrophoresis spectrum, and judging whether there is an absorption peak of coumarin in the sample capillary electrophoresis spectrum, so as to determine whether the sample solution to be detected contains coumarin.

The invention discloses an aedes albopictus drug resistance related gene mutation detection method based on a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) technology, and belongs to a molecular detection technology, the aedes albopictus drug resistance related gene mutation detection method comprises the following steps: S1, extracting a genome DN A of single-head aedes albopictus, the genotype of a resistance related mutation site of the single-head aedes albopictus is known; s2, respectively obtaining electrophoretograms of resistance-related mutation site genotypes: firstly carrying out PCR amplification reaction, then carrying out enzyme digestion on a PCR amplification product, and carrying out electrophoresis detection on an enzyme digestion product; primers for PCR (Polymerase Chain Reaction) amplification of the VGSC 1016 are SEQ ID NO: 3 and SEQ ID NO: 4, and primers for amplification of the VGSC 1532 are SEQ ID NO: 7 and SEQ ID NO: 8; and S3, establishing a standard diagram. The method disclosed by the invention can be used for rapidly and effectively detecting the resistance of the aedes albopictus to the pyrethroid insecticides.

The application discloses a primer for amplifying a Bellamya aeruginosa gender-related molecular marker and application of the primer in Bellamya aeruginosa gender identification, and belongs to the technical field of aquatic animal genetics and breeding. The Bellamya aeruginosa gender-related molecular marker disclosed by the application is obtained by whole genome resequencing and GWAS analysis screening; the molecular marker is SEQ ID NO. 1 and SEQ ID NO. 2; and the gender identification method is, on the basis of designing specific primers for the molecular marker, taking genomic DNA as a template, performing PCR amplification and 3% agarose gel electrophoresis, and on an electrophoretogram, a band for a female and two bands for a male. The application can accurately and quickly identify the genetic gender of the Bellamya aeruginosa and does not need sequencing. The application has important application value in gender identification in Bellamya aeruginosa artificial breeding and breeding practice and the like.

The invention discloses an AS-PCR primer and application of the AS-PCR primer to detection of drug resistance of litchi colletotrichum gloeosporioides to azoxystrobin. The invention provides an AS-PCR primer, and develops a method for detecting the drug resistance of litchi colletotrichum gloeosporioides to azoxystrobin based on the primer. The method for detecting the drug resistance of the colletotrichum litchis to the azoxystrobin is simple and convenient to operate, only the DNA of the colletotrichum litchis needs to be extracted and then PCR amplification and electrophoretic analysis are carried out, a single target band can be observed in a drug-resistant strain in an electrophoretogram, a sensitive strain does not have an amplified band, and the method is suitable for large-scale sample screening. In addition, the primer designed by the invention has high specificity, not only can accurately distinguish drug-resistant and sensitive strains, but also has no cross amplification on other common plant pathogenic fungi. Reliable technical support is provided for early rapid diagnosis of drug resistance of the litchi colletotrichum gloeosporioides to azoxystrobin, and high practical value and wide application prospect are achieved.

The invention belongs to the technical field of dairy product detection, and particularly relates to a method for detecting the proportion and activity of each whey protein in milk and dairy products through a fluorescent gel electrophoresis technology. Carrying out lauryl sodium sulfate-polyacrylamide gel electrophoretic analysis on the milk and dairy products marked by the protein fluorescent dye, firstly adding concentration gel for concentration, compressing the protein dispersed in a sample loading hole into a narrow sample band, then adding separation gel for separation, and according to the difference of molecular weights of different whey proteins and casein, determining the content of the whey protein and the casein. According to the method, whey proteins are separated from each other, migration rates in gel are different, effective separation is realized, finally, an electrophoretogram is obtained through a gel imaging system, a gray value of the electrophoretogram is subjected to quantitative processing by adopting gel image analysis software, and the proportion and activity of each whey protein are obtained through calculation. Compared with a traditional Coomassie brilliant blue dyeing method, the method is high in marking speed, does not need time-consuming dyeing and decolorizing steps, and is high in practicability.

The invention relates to the technical field of image processing, in particular to a high-voltage power supply DNA sequencing visual detection method and system.The method comprises the steps that a Sanger sequencing technology is adopted to obtain DNA electrophoretograms of four channels; obtaining each contour in the DNA electrophoresis grayscale image; constructing a DNA local density confidence coefficient and a local DNA density parameter of each pixel point according to the gray values of each pixel point and a neighborhood pixel point in the channel; acquiring a density run-length matrix of each pixel point; constructing a DNA density distribution heterogeneity coefficient and a channel trailing effect evaluation parameter of each pixel point, and constructing a sequencing segmentation probability coefficient of each pixel point based on the DNA density distribution heterogeneity coefficient and the channel trailing effect evaluation parameter; obtaining a decision threshold value of each pixel point; and taking the center of each contour as a seed point, obtaining each DNA fragment according to a region growing method based on an adaptive threshold value, and further obtaining a DNA sequence. According to the invention, the accuracy of detecting the base sequence of the DNA sequence can be improved.

The invention provides an intelligent electrophoretogram interpretation method fusing YOLO and ConvNeXt, and belongs to the crossing field of medical artificial intelligence and digital inspection science, and the method comprises the steps: obtaining a whole IFE electrophoresis plate scanning image; using a YOLOv11 target detection model to position a case related stripe region, and outputting a candidate box; the candidate boxes are verified and sorted through a grid sensing sorting module, and standardized cutting is executed; the cut ROI is input into a ConvNeXt multi-label classification network, and five-dimensional biological indexes are output in parallel; and mapping the network output into a clinically readable conclusion. According to the method, the whole-board-level end-to-end automatic interpretation is achieved, the interpretation accuracy rate reaches 94.67%-98.67%, the AUC value is 0.97, the batch processing efficiency and cross-scene consistency are remarkably improved, and the method is suitable for clinical IFE map large-scale preliminary screening and follow-up visit management. The method aims at solving the problems that existing immunofixation electrophoretogram interpretation depends on manual pretreatment, the automation degree is low, the interpretation consistency is poor, and model maintenance is difficult.

The invention relates to the technical field of N sugar chain detection, in particular to a method for detecting N sugar on bird's nest glycoprotein, which comprises the following steps: acquiring the length of a plurality of N sugar chains in historical data; determining the rotating speed of a centrifugal machine in the buffer solution cleaning process; outputting a purified glycoprotein solution; determining whether the bird's nest glycoprotein is broken or not; adjusting the time interval between the purification end time of the cubilose protein and the adding time of the hydrolase, or determining the pH value of the denaturation liquid; cutting and releasing an N sugar chain; outputting the marked N sugar chain solution, and outputting a purified N sugar chain solution; determining sample loading voltage in the electrophoretic separation process; performing electrophoretic separation on the purified N sugar chain solution to obtain an electrophoretogram, and analyzing the electrophoretogram to obtain N sugar chain structure information of the bird's nest glycoprotein. According to the method, the detection accuracy of the N sugar chain in the bird's nest glycoprotein is improved.

The invention discloses a method for unmarked quantitative distinguishing of the stoichiometric ratio of an antibody nucleic acid coupling probe. The method comprises the following steps: 1) dissolving the antibody nucleic acid coupling probe in a CZE separation buffer solution to prepare a sample solution; 2) setting proper capillary zone electrophoresis conditions, and separating the sample by using a capillary zone electrophoresis apparatus; 3) monitoring the electrophoretic separation process in real time through an ultraviolet detector or a mass spectrum detector; and (4) realizing the stoichiometric ratio of the antibody nucleic acid coupling probe for label-free quantitative analysis according to the peak position and peak area in the electrophoretogram. According to the invention, based on the capillary zone electrophoresis technology, accurate and convenient analysis of oligonucleotide-antibody stoichiometric ratios obtained by different coupling methods is realized, and even coupling products with the difference of only one oligonucleotide can be distinguished. The method provides an important tool for quality control and optimization of the AOCs, and is expected to promote further application of the AOCs in the field of biomedicine.

The invention relates to a method for determining the average coupling rate of an antibody-coupled drug based on capillary gel electrophoresis and deglycosylation pretreatment and application thereof, and the method comprises the following steps: carrying out deglycosylation and reduction pretreatment on an antibody-coupled drug test sample, carrying out capillary gel electrophoresis, carrying out peak attribution on an electrophoretogram, and determining the average coupling rate of the antibody-coupled drug according to the peak attribution. And calculating the average coupling rate of the antibody coupling drug. According to the method, enzymatic deglycosylation pretreatment is combined with the CGE, and meanwhile, deglycosylation reaction conditions are optimized, so that the separation effect is remarkably improved, the complexity of a CE-SDS electrophoresis spectrogram is reduced, and the accuracy of ADC molecule average coupling rate analysis is greatly improved. The method is suitable for high-throughput analysis requirements in antibody coupling drug research and development, production quality control and stability research.

The invention relates to a method for verifying an electroluminescent security feature (2) in a value and / or security document (3) comprising the following steps: Exciting the electroluminescent security feature (2) using an electrical excitation signal (8), detecting radiation emitted by the security feature (2) and generating an output signal (11), evaluating the output signal (11) and deriving a verification decision, and issuing the verification decision, wherein in addition, while the security element (2) is excited to detect the emitted radiation, the security element (2) is irradiated with light radiation (31) in the UV wavelength range. Furthermore, the invention relates to a device for verification.

The invention relates to a method for identifying hucho taimen and hucho bleekeri, which comprises the following steps: collecting fin rays or scales of sample fish, and splitting to obtain a DNA crude extract; carrying out PCR (Polymerase Chain Reaction) amplification by using the primer by taking the DNA crude extract as a template, and carrying out electrophoresis detection on an amplification product; the obtained electrophoretogram is compared with the standard chromatogram of hucho taimen and hucho bleekeri, if the chromatogram is provided with two strips, the sample is the hucho taimen, and if the chromatogram is provided with a single strip, the sample is the hucho bleekeri. The identification method provided by the invention is small in damage to fish bodies, can be used for identifying the fries, solves the problems that the fries are mixed and commercial fishes are difficult to identify in the enhancement and release process, and has the advantages of simplicity, rapidness, accuracy, economy, practicability and the like.

The invention relates to the field of antibodies, in particular to an anti-adhesion protein monoclonal antibody HK02TJ and application thereof, and it can be seen that the monoclonal antibody has excellent performance in adhesion protein detection through an electrophoretogram obtained after antibody purification and performance analysis of a kit containing the monoclonal antibody. The method can be used as an object for further production and optimization.

The invention discloses application of a primer for amplifying a molecular marker related to sex of Bellamya piriformis in sex identification of Bellamya piriformis, and belongs to the technical field of genetic breeding of aquatic animals. The molecular marker related to the gender of the Bellamya piriformis, disclosed by the invention, is obtained through whole genome re-sequencing and GWAS analysis and screening; the molecular markers are as shown in SEQ ID NO. 1 and SEQ ID NO. 2; the sex identification method comprises the following steps: on the basis of designing a specific primer aiming at the molecular marker, carrying out PCR (Polymerase Chain Reaction) amplification and 3% agarose gel electrophoresis by taking genome DNA (Deoxyribonucleic Acid) as a template, and on an electrophoretogram, the female has one strip, and the male has two strips. The method can accurately and rapidly identify the genetic sex of the Bellamya piriforme, and does not need sequencing. The method disclosed by the invention has important application value in sex identification of artificial propagation, breeding practice and the like of the Bellamya piriformis.

The invention discloses a method for detecting coumarin, which comprises the following steps: (1) preparing the following solutions: a borax aqueous solution, a NaOH aqueous solution, a boric acid aqueous solution and a 2, 6-dichloroquinone-4-chloroimine ethanol solution; (2) adjusting the pH value of the borax aqueous solution to 11.8-12.2 by using a NaOH aqueous solution to obtain a reaction buffer solution; (3) uniformly mixing the reaction buffer solution and the to-be-detected sample solution according to a volume ratio of 2: 1, and reacting under a water bath heating condition; (4) adjusting the pH value of the solution to 8.6-9.0 by adopting a boric acid aqueous solution and pure water, adding a 2, 6-dichloroquinone-4-chloroimine ethanol solution, uniformly shaking, and placing at room temperature for color development to obtain a to-be-detected color development sample solution; and (5) detecting the to-be-detected chromogenic sample solution by using the capillary electrophoresis apparatus to obtain a sample capillary electrophoresis pattern, and judging whether the absorption peak of coumarin exists in the sample capillary electrophoresis pattern so as to determine whether the to-be-detected sample solution contains coumarin.

The application discloses a capillary serum protein electrophoresis atlas partition method, device, medium and equipment, and belongs to the M protein analysis field. The application firstly acquires a serum protein electrophoresis curve of M protein and performs effectiveness judgment, calculates a candidate peak group of the serum protein electrophoresis curve and an abscissa position of the candidate peak group after the effectiveness judgment passes, then determines three candidate regions according to the number and the abscissa position of the candidate peak group, and calculates the ordinate minimum value of the three candidate regions and corresponding abscissa positions fl, sl and tl, and determines the beta region and the gamma region according to the abscissa positions fl, sl and tl. The application can automatically divide the beta region and the gamma region of a serum protein electrophoresis control atlas according to the serum protein electrophoresis curve, is more intelligent and efficient, and does not need manual adjustment, and when analyzing a complex serum protein electrophoresis control atlas, the beta region and the gamma region can be more accurately divided.

The application discloses a gel electrophoresis identification method for leeches and leech worms and their close relatives and application of the method, and relates to the technical field of traditional Chinese medicine identification. The method comprises the following steps: (1) pre-treating a leech sample to be detected to obtain a sample solution of the leech sample to be detected, and pre-treating a control medicinal material to obtain a solution of the control medicinal material; wherein the control medicinal material is a leech, a leech worm and / or a close relative and fake product thereof; (2) performing sodium dodecyl sulfate polyacrylamide gel electrophoresis and gel imaging analysis on the sample solution of the leech sample to be detected and the solution of the control medicinal material to obtain a gel electrophoresis spectrum of the leech sample to be detected and a gel electrophoresis spectrum of the control medicinal material; and (3) comparing the above spectra, and determining whether the leech sample to be detected is a leech, a leech worm or a close relative and fake product thereof according to a comparison result; and the leech sample to be detected is selected from one or more of the following: a leech, a leech worm, a limnatis filamentaria, a haemopis japonica and a haemopis sanguinea. The method is intuitive, accurate and easy to identify.