The invention provides a method for extracting
protein from cotton leaves. The method comprises the following steps of 1)
grinding the cotton leaves; 2) transferring the ground leaves
powder to a centrifugal
pipe, adding
trichloroacetic acid extraction liquid, uniformly mixing, and storing the mixture in a refrigerator with the temperature of -20 DEG C for more than 2h; 3) centrifuging in the centrifugal
pipe, removing the supernatant, retaining the precipitate, adding
acetone solution pre-cooled at the temperature of -20DEG C into the precipitate, uniformly mixing, and standing for 2h to 4h at the temperature of -20DEG C; 4) centrifuging in the centrifugal
pipe, removing the supernatant, retaining the precipitate, washing the precipitate, and retaining the precipitate; 5) vacuumizing the precipitate, and volatizing
acetone to obtain dry
protein powder; 6) adding
protein lysate into the dry protein
powder, disintegrating for 1h in the water bath with temperature being 35DEG C, centrifuging, and taking the supernatant which is the cotton leave protein dissolved liquid. By adopting the method for extracting the protein from the cotton leaves, the extracted protein is stable, the
repeatability is good, the purity of the protein is high, and the
electrophoretogram show that no grain phenomena such as the cross stripes and streaking appear.