The invention discloses a specific primer used for
rapid detection of a
kiwi fruit soft rot pathogenic bacterium, and a detection method of the
kiwi fruit soft rot pathogenic bacteriaum. A problem that existing technology is incapable of realizing accurate and
rapid detection of the
kiwi fruit soft rot pathogenic bacterium is solved. The specific primer comprises BZY-1 and BZY-2, the sequence of BZY-1 is 5'-CCATCAAACTCCAGTCAGTAAACG-3', and the sequence of BZY-2 is 5'-CTGCGCTCCGAAGCGAGATGTATG-3. The detection method of the
kiwi fruit soft rot pathogenic bacterium comprises following steps: (1)
DNA of samples are extracted; (2) the sample
DNA is taken as a template, and primer BZY-1, primer BZY-2, 2*TaqPCRMasterMix and sterile
ultrapure water are added for uniform mixing, and then PCR amplification is performed; and (3) an amplification product is subjected to
agarose gel electrophoresis analysis, and the existence of the
kiwi fruit soft rot pathogenic bacterium is determined based on the existence of amplification bands. Specificity of the primer is extremely high, only the
kiwi fruit soft rot pathogenic bacterium band can be obtained by amplification, so that it is just needed to extract
genome DNA from kiwi fruit tissue to be detected for PCR amplification, and the existence of the kiwi fruit soft rot pathogenic bacterium is determined based on the existence of amplification bands in electrophoretograms.