Specific primer used for rapid detection of kiwi fruit soft rot pathogenic bacterium Botryosphaeria dothidea, and detection method of Botryosphaeria dothidea

Abstract

The invention discloses a specific primer used for rapid detection of a kiwi fruit soft rot pathogenic bacterium, and a detection method of the kiwi fruit soft rot pathogenic bacteriaum. A problem that existing technology is incapable of realizing accurate and rapid detection of the kiwi fruit soft rot pathogenic bacterium is solved. The specific primer comprises BZY-1 and BZY-2, the sequence of BZY-1 is 5'-CCATCAAACTCCAGTCAGTAAACG-3', and the sequence of BZY-2 is 5'-CTGCGCTCCGAAGCGAGATGTATG-3. The detection method of the kiwi fruit soft rot pathogenic bacterium comprises following steps: (1) DNA of samples are extracted; (2) the sample DNA is taken as a template, and primer BZY-1, primer BZY-2, 2*TaqPCRMasterMix and sterile ultrapure water are added for uniform mixing, and then PCR amplification is performed; and (3) an amplification product is subjected to agarose gel electrophoresis analysis, and the existence of the kiwi fruit soft rot pathogenic bacterium is determined based on the existence of amplification bands. Specificity of the primer is extremely high, only the kiwi fruit soft rot pathogenic bacterium band can be obtained by amplification, so that it is just needed to extract genome DNA from kiwi fruit tissue to be detected for PCR amplification, and the existence of the kiwi fruit soft rot pathogenic bacterium is determined based on the existence of amplification bands in electrophoretograms.

Description

technical field [0001] The invention relates to a method for detecting plant pathogenic fungi, in particular to a method for rapidly detecting kiwifruit soft rot pathogens Botryosphaeria dothidea The specific primers and detection method thereof are used to quickly and accurately detect whether there is kiwifruit soft rot pathogen in kiwifruit fruit. Background technique [0002] Post-harvest fruit rot is a global problem, and more than 25% to 30% of fresh fruits worldwide cannot be used due to rot and deterioration during storage. Postharvest fruit rot is mainly caused by pathogen infection during postharvest processing, transportation, storage and sales of fruits. With the development of my country's fruit industry and the expansion of planting area, postharvest fruit rot has gradually been paid attention to. However, due to incomplete preharvest disease control and backward storage and transportation technology, the situation of postharvest fruit rot is increasing d...

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a method for rapid detection of kiwifruit soft rot pathogenic bacteria Botryosphaeria dothidea The specific primers and detection methods thereof mainly solve the problem that the existing technical means cannot quickly and accurately detect the presence of kiwifruit soft rot pathogens in kiwifruit

Images