70results about How to "Efficient detection means" patented technology

A ship loader level two-dimensional laser scanning radar automatic detecting system and a method relates to the automatic detecting technical field, wherein, the system comprises two sets of laser scanning devices, an industrial control main frame and an image pick-up device; each laser scanning device comprises a two-dimensional laser radar, a servomotor, a shaft coupling, a rotating main shaft, a limit and zero sensor, a bearing seat containing a bearing, a contracting brake device, a laser radar back support and an L-shaped mounting bottom plate; the image pick-up device comprises a pickup camera and a tripod head. By means of the realtime scanning of the two-dimensional laser radar, the invention collects related data of a ship cabin, a loading chute and material; meanwhile, the invention provides a device realizing realtime display of the status of the ship cabin, the loading chute and material stacking as well as corresponding detection and data processing method. The system realizes three-dimensional scanning of the ship loader and material by means of the two-dimensional laser scanning radar which is combined with the servo motor and a motion control card. In addition, the invention can realizes less than 200mm positioning error of hatchway position and less than 100mm surface topography identification error.

The invention discloses a screening method for 110 types of medicines in feed, which specifically includes the following steps: preparation of standard work solution, pre-analysis treatment of sample to be assayed, creation of database and qualitative analysis. By utilizing the high-sensitivity MSMS (Tandem Mass Spectrometry) function of a liquid chromatography-tandem quadrupole time-of-flight mass spectrometer, the screening method can accurately determine parent ions and daughter ions in medicines and rapidly determine added medicine ingredients, moreover, an assay method for a variety of antibacterial medicines in feed is also established, and for a variety of added antibacterial medicines in the feed industry in China, the screening method has a highly efficient, accurate and precise assay measure, and is at the advanced level in china at present. The screening method established by the invention greatly solves the phenomenon of arbitrary addition in the feed industry, the more precise and accurate assay method is perfected for the inspection department, more time is saved, most of experimental consumables are saved, and the invention makes an immeasurable contribution to food safety and human healthy life.

An enzyme-linked immunoassay method of bisphenol A belongs to the technical field of immunodetection. The enzyme-linked immunosorbent assay method utilizes the immunization of a synthetic bisphenol A immunogen to obtain a polyclonal antibody, takes the bisphenol A as a standard product, takes a conjugate of a hapten of diphenolic acid and OVA as a coating antigen and establishes the indirect competitive enzyme-linked immunosorbent assay method of the bisphenol A. The enzyme-linked immunosorbent assay method establishes the indirect competitive ELISA method of the bisphenol A and provides a rapid and high-efficient detection method for detecting the residual bisphenol A, as the method adopts the polyclonal antibody, the cost is lower, and the stability and the repeatability are good. The sensitivity is 0.1ng/ml, and the linear range is 0-100ng/ml. The high specificity and the affinity of the immune reaction lead the ELISA to have very high selectivity and sensitivity.

The invention discloses an enzyme-linked immunosorbent assay method of paraoxon-methyl, belonging to the technical field of immunoassay. The enzyme-linked immunosorbent assay method utilizes a synthetic paraoxon-methyl immunogen for immunization, thereby obtaining a polyclonal antibody; the paraoxon-methyl is taken as a standard product, and a conjugate of the paraoxon-methyl hapten and OVA is taken as a coating antigen, thereby establishing the indirect competitive enzyme-linked immunosorbent assay method of the paraoxon-methyl in textiles (cotton). The enzyme-linked immunosorbent assay method establishes the indirect ELISA method of the paraoxon-methyl pesticide metabolite in the textiles, thereby providing the rapid and high-efficient detection means for the residual detection of the paraoxon-methyl in the textiles; as the enzyme-linked immunosorbent assay method adopts the polyclonal antibody, the cost is lower, the stability and the repeatability are better. The sensitivity is 0.01ppm and the linear range is 0.1 to 20ppm. The high specificity and the high affinity of the immune reaction allow the ELISA to have very high selectivity and sensitivity.

The invention relates to a rhodamine B ELISA detection method, which belongs to the technical field of ELISA. The invention discloses a rhodamine B ELISA detection method, which comprises the following steps: using synthesized rhodamine B immunogen for immunizing healthy New Zealand rabbits to obtain polyclonal antibodies; and using rhodamine B as standard products; using rhodamine B hapten and OVA coupling materials as coating antigen to establish a rhodamine B indirect ELISA method. The invention provides the fast and efficient detection method for the residual detection on the rhodamine B. Because the polyclonal antibodies are adopted, the cost is low, and in addition, the stability and the repetitiveness are good. The detectability (IC90) is 0.028 ng/mL, the half inhibitory amount (IC50) is 1.3 ng/mL, and the detection range (IC20 to IC80) is between 0.07 and 17.5 ng/mL. Because of high specificity and high compatibility of the immune reaction, high selectivity and high sensitivity can be obtained when the ELISA is used for detecting the rhodamine B.

The invention discloses an indirect competition ELISA method for tetrabromo bisphenol A. The assay method comprises the following methods: 1) synthesizing an artificial antigen through the tetrabromo bisphenol A; 2) preparing a tetrabromo bisphenol A polyclonal antibody: taking the conjugate of the tetrabromo bisphenol A and BSA as an immunizing antigen, immunizing Kunming white rats, and utilizing the conventional polyclonal antibody preparation and purification technologies to acquire the tetrabromo bisphenol A polyclonal antibody; 3) assaying the indirect competition ELISA: choosing an envelope antigen of which the concentration is 10 [mu]g/mL, and performing dilution with a carbonate buffer solution; washing an ELISA plate, adding the buffer solution to seal the ELISA plate and washing the ELISA plate again; carrying out competition, washing the ELISA plate, adding a second antibody and washing the ELISA plate again; performing a chromogenic reaction; finally, measuring with a microplate reader to acquire that a light absorption value is 450nm (OD 450). The invention constructs the indirect competition ELISA method for the tetrabromo bisphenol A, and provides a quick and efficient assay means for assaying the residual tetrabromo bisphenol A in atmosphere; as the method adopts the polyclonal antibody, the cost is lower and the stability is better.

The invention discloses a peste des petits ruminants virus recombinant protein antigen and a rapid test strip for detecting a peste des petits ruminants virus antibody by using the recombinant protein antigen as a detection line reagent. The peste des petits ruminants virus recombinant protein antigen can be obtained by the following steps: selecting main antigenic epitope-containing amino acid segment SEQ IDNo.1 through analyzing the N protein antigenic epitope of the peste des petits ruminants virus, optimizing a codon and artificially synthesizing the codon-optimized gene sequence SEQIDNo.2, constructing a recombinant expression carrier and transforming Escherichia coli for protein expression. The purified peste des petits ruminants virus recombinant protein antigen can be used for detecting the peste des petits ruminants virus antibody. The rapid test strip established based on the peste des petits ruminants virus recombinant protein antigen has the advantages of being convenient to operate, fast to detect, free from special laboratories, equipment and the like, overcomes the limit of the existing detection method, and can be used for rapid detection of the peste des petits ruminants virus antibody and investigation of serum epidemiology.

The invention belongs to the biotechnology field and discloses a ctenopharyngodon idellus interleukin-1beta (IL-1beta) competitive inhibiting enzyme-linked immunosorbent assay (ELISA) kit. The kit comprises the following components: (1) an ELISA plate, (2) a horse radish peroxidase marked ctenopharyngodon idellus IL-1beta polyclonal antibody concentrated solution, (3) a sample diluent, (4) washing concentrate, (5) concentrated coating buffer, (6) a chromogenic substrate, (7) stop buffer and (8) standard ctenopharyngodon idellus IL-1beta. The kit has the characteristics of simplicity in operation, good repeatability, accuracy and specificity and wide linear detection range.

The invention relates to the technical field of biology, and in particular relates to a kit for combined detection of 6 diabetic antibodies. The kit is used for combined detection of 6 antibodies such as ICA, GADA, IAA, IA-2ACPH-A and ZnT8-A in T1DM, T2DM and serum of a normal person, has high sensitivity (98 percent) and specificity (99.6 percent) for I type diabetic diagnosis, and provides efficient and fast detection means for classificatory diagnosis of diabetes.

The invention relates to a competitive inhibition enzyme-linked immunoassay (ELISA) method of grass carp interleukin 10 (IL-10). The method comprises the following steps of: immunizing a New Zealand white rabbit by utilizing grass carp interleukin 10 proteins which is expressed in a recombined way as an immunogen to obtain a polyclonal antibody; marking the antibody by utilizing horseradish peroxidase; and finally, establishing the competitive inhibition ELISA method by taking the recombined grass carp interleukin IL-10 protein as a covered antigen, wherein the competitive inhibition ELISA method can be used for the protection detection of the grass carp interleukin 10. The detection range of the ELISA method is 0.1-100 ng/mL. By utilizing the polyclonal antibody and the competitive inhibition ELISA method, the cost is low, and the stability and the repeatability are good. The conventional method is that the competitive inhibition ELISA method is firstly established to detect protection expression of the grass carp interleukin 10.

The invention relates to an enzyme-linked immunoassay method for dioctylphthalate, and belongs to the technical field of immunodetection. The invention utilizes immunity of synthesized dioctylphthalate immunogen to obtain a polyclonal antibody, and takes the dioctylphthalate as standard and conjugate of dioctylphthalate hapten and OVA as envelope antigen, to establish indirect competitive enzyme-linked immunoassay method for the dioctylphthalate. The invention establishes the indirect competitive ELISA method for the dioctylphthalate, and provides a quick and high-efficiency detecting method for detecting residual of the dioctylphthalate. The method adopts the polyclonal antibody, so the cost is lower and the stability and repetitiveness are good; the sensitivity is 0.01ng/mL, and the linear range is between 0.1 and 19ng/mL; and high specificity and compatibility of an immunoreaction make the ELISA have extremely high selectivity and sensitivity.

The invention discloses a hapten and an artificial antigen of florfenicol and analogues thereof, a monoclonal antibody, and an enzyme-linked immunosorbent assay (ELISA) method of the florfenicol and analogues thereof. The hapten of the florfenicol is firstly prepared by deriving succinic acid half-ester coupling arm from benzyl hydroxyl of florfenicol molecules; the hapten is further coupled withcarrier protein, so that the artificial antigen of the florfenicol is obtained; after that, an animal is immunized with the artificial antigen, so that the monoclonal antibody for the florfenicol is obtained; the monoclonal antibody can be used for detecting the florfenicol and the analogues such as florfenicol amine and thiamphenicol. The ELISA method of the florfenicol is established by using the monoclonal antibody and can be used for detecting the specificity of florfenicol residues; the IC50 of the method for the florfenicol is equal to 5.85ng/mL, and the linear detection range is 1.67-20.49ng/mL, so that the method has the remarkable advantages of being high in sensitivity, good in specificity, and the like; the method can be used for rapidly detecting the residual florfenicol in food, and has a wide application prospect.

The invention discloses a wireless adaptive temperature sensor with functions of network testing and displaying, which is sensor equipment with a network signal monitoring function belonging to the technique of low-consumption wireless sensors and developed to meet requirements of common families and institutions on real-time environment monitoring. The sensor program adopts a design scheme including a universal single-chip microcomputer, a liquid control circuit and a wireless ZIGBEE network module. The single-chip microcomputer realizes displaying and intelligent environment monitoring at low consumption by means of enabling management and hibernation algorithm of a power chip, and functions of acquiring the current status of network communication by users, managing a power switch of the sensor, transmitting current displayed temperature readings and the like can be realized easily with external keys. The more advanced ZIGBEE network in wireless sensor networks is adopted, so that data reliability and networking scale adaptability are guaranteed. A display module is designed to better realize a user interaction function of the sensor, while the Kalman filtering algorithm is added to achieve intelligent adjustment of transmitting intervals of nodes, and accordingly true risks of data with transmitting intervals are lowered greatly, operation of a real-time system is more benefited, a function of indoor environment alarming is realized for users, and accordingly the wireless adaptive temperature sensor is capable of detecting temperature and assuming important responsibility of family security.

The invention discloses a florfenicol and florfenicol amine antigen, antibody and a simultaneous detection enzyme-linked immuno sorbent assay analytical method thereof. The simultaneous detection enzyme-linked immuno sorbent assay analytical method utilizes florfenicol as a hapten to be coupled with a carrier protein to obtain an artificial antigen, and the artificial antigen is used as a coatingantigen; meanwhile, the hydroxyl group of the florfenicol molecule derives the succinic acid half ester to couple with arm to obtain the florfenicol hapten, and the florfenicol artificial antigen is obtained as an immunogen by coupling with the carrier protein; the detection antibody is prepared, and the enzyme-linked immuno sorbent assay analytical method is established. The IC50 of florfenicol is 37.10ng/mL, the linear detection range is 12.03-114.49ng/mL, the IC50 of florfenicol amine is 45.3ng/mL, and the linear detection range is 10.76-190.69ng/mL. The method can be used for quickly detecting the residual florfenicol and florfenicol amine in the food, and has wide application prospect.

The invention discloses enzyme-linked immunoassay method for benzophenone, and belongs to the technical field of toxic chemical residue detection. The indirect competitive enzyme-linked immunoassay method for benzophenon in a packing material is established by immunizing by using synthesized 4-aminobenzophenone and a BSA coupling substance immunogen to obtain a polyclonal antibody, taking 4-aminobenzophenone hapten and a coupling substance of OVA as coating antigens and taking the benzophenone as a standard substance. The invention provides a quick and high-efficiency means of detecting the benzophenone residue in the packing material. Because the polyclonal antibody is adopted, the cost is relatively low and the stability and the repeatability are relatively good. The sensitivity is 0.5ppm and the linear range is between 1 and 100ppm. The ELISA has extremely high selectivity and sensitivity due to high specificity and affinity of an immune reaction.

The invention relates to a method for rapidly detecting alarm link failure in IP (internal protocol)-RAN (random access network) equipment. The method comprises the steps of: making a quick alarming link detecting protocol including a request message (request) and a reply message (replay); periodically sending the request message (request) to a main control unit through the business interface unit of the IP-RAN equipment; and if the business interface unit receives the reply message (replay) and can correctly fetch field information from the reply message (replay), determining that the link for the message transmission is correct based on physics and logic; otherwise, determining the link as the chain in an abnormal state. By using the method provided by the invention, the defects that the alarming is out of work because the alarming can not be normally transmitted due to abnormal link are prevented during researching, developing, producing, managing and maintaining IP-RAN equipment; and a mechanism for quickly detecting the alarming link fault between boards is provided to achieve efficient, reliable and flexible alarming link detecting means, which has important implications for ensuring the stability of the business.

The invention discloses a monoclonal antibody capable of specifically identifying tenuazonic acid and its enzyme-linked immunosorbent assay method. The monoclonal antibody of tenuazonic acid is prepared by firstly preparing hapten (TEA-CMO), and then respectively coupling hemocyanin (KLH) and bovine serum albumin (BSA) to obtain immunogen (TEA-CMO-KLH) and package (TEA-CMO-BSA); then applying animal immunization and hybridoma technique to obtain the monoclonal antibody of tenuazonic acid; an indirect competition enzyme-linked immunosorbent assay method built by the monoclonal antibody of tenuazonic acid is a rapid detecting method of tenuazonic acid residue; the detection limit (IC10) of the method is 1.00 ng/mL, IC50=18.50 ng/mL; the linear detection scale is 3.56-96.24 ng/mL; the direct detection of the tenuazonic acid can be realized; the monoclonal antibody is free from crossing reaction with similar matters of another nine alternaria alternata aflatoxin structures; the method is good in specificity and high in sensitivity.

The present invention discloses one kind of permethrine polyclonal antibody enzyme-linked immunoassay method. Synthetic permethrine immune antigen is utilized in immunizing health rabbit to obtain polyclonal antibody, and after screening, the indirect competitive enzyme-linked immunoassay method for detecting residual permethrine in soil and food is established with permethrine as standard article and permethrine semi-antigen and OVA conjugate as coating antigen. The method is fast and efficient, and has relatively low cost, high stability and high repeatability, sensitivity of 0.1 ppb, linear range of 10-800 ppb, high selectivity and simple sample pre-treatment.

The invention discloses a chiral compound with a selenourea group structure, and also provides a preparation method and application of the selenourea chiral compound. The structure of the new compound is shown as the specification. The compound is used as a chiral shift reagent to detect the optical purity of chiral carboxylic acid, chiral alcohol, chiral amino alcohol and other chiral compounds, has the characteristics of simple operation and large chemical shift difference, thus being a rapid, efficient, convenient and practical detection means.

The invention discloses a rapid detection test strip for an epizootic hemorrhagic disease virus (EHDV) antibody and a preparation method of the rapid detection test strip. The rapid detection test strip mainly adopts a truncated EHDV VP7 expression antigen as a detection line reagent. Antigen epitope software is used for analyzing EHDV VP7 and a segment containing a main antigen epitope is selected to optimize a codon corresponding to the segment; a DNA (Deoxyribonucleic Acid) segment is artificially synthesized according to an optimized nucleotide sequence and a recombinant expression carrier is constructed; colon bacillus is transferred to carry out protein expression to obtain a truncated EHDV VP7 recombinant antigen; the truncated EHDV VP7 recombinant antigen is purified and then is used for detecting the EHDV antibody. The rapid detection test strip established based on the truncated EHDV VP7 expression antigen has the advantages of convenience for operation, rapidness in detection, no need of special laboratories and equipment and the like; the limitation of an existing detection method is overcome; the rapid detection test strip can be used for rapid detection on the EHDV antibody and blood serum epidemiological investigation.