1results about How to "Efficient detection means" patented technology

The application belongs to the technical field of gene detection, and particularly relates to specific primers, a Taqman probe and application of a bacillus velezensis HMB26553 strain. The specific primers and the probe sequence of the bacillus velezensis HMB26553 strain are respectively SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3. The primers can amplify a single unique target fragment from the bacillus velezensis HMB26553 strain, and realize specific detection of the bacillus velezensis HMB26553 strain. The primers have the characteristics of high specificity, high detection efficiency, low false positive rate, long preservation time and the like. By using the primers and through real-time quantitative PCR technology, the population quantity of the bacillus velezensis HMB26553 strain in the rhizosphere of cotton can be quickly quantified, and the primers have the advantages of high throughput, rapidness and accuracy.