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Method for labeling and identifying Szechwan white geese by utilizing ISSR (inter simple sequence repeat)

A technology of Sichuan white goose and marking, applied in the field of livestock and poultry genetic resource identification, can solve the problems of identification of different goose breeds that are not used, and achieve the effects of easy production and promotion, strong stability and enhanced repeatability

Inactive Publication Date: 2013-09-04
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the study of geese, there are studies on ISSR molecular markers of the main meat production performance of Jilin White Goose and the application of ISSR molecular markers on the genetic diversity of geese and geese, but they are not used to identify different goose varieties

Method used

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  • Method for labeling and identifying Szechwan white geese by utilizing ISSR (inter simple sequence repeat)
  • Method for labeling and identifying Szechwan white geese by utilizing ISSR (inter simple sequence repeat)
  • Method for labeling and identifying Szechwan white geese by utilizing ISSR (inter simple sequence repeat)

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Experimental program
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Effect test

Embodiment

[0029] Embodiment: To identify whether a group of geese or introduced goslings are true Sichuan white geese.

[0030] (1) Collection of blood samples or tissue samples Randomly take 3 geese to be identified, collect 2-3ml of blood from each goose wing vein, add 0.5% heparin sodium for anticoagulation, centrifuge at low temperature and low speed for 5min, carefully remove the upper layer of plasma, and place in- Store in a refrigerator at 20°C for later use; or cut about 0.1g of tissue samples from goose webs and freeze for later use.

[0031] (2) Extraction of total DNA from goose genome Take 20 μl of blood after thawing, or take about 0.05 g of tissue sample, add 500 μl of lysate, 10 μl of 10% SDS, 5 μl of RNaseA (20 μg / μl), 10 μl of proteinase K ( 20mg / mL), mix well, 55 ℃ water bath overnight; add an equal volume of Tris saturated phenol, shake for 20min, centrifuge at 12000rpm for 10min; remove the supernatant, add an equal volume of phenol / chloroform / isoamyl alcohol (25:24...

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Abstract

The invention belongs to the field of livestock or poultry genetic resource identification, and in particular relates to a method for identifying true or false of Szechwan white geese by utilizing an ISSR (inter simple sequence repeat) molecular marker technique, wherein three ISSR labels are used totally, and standard electrophoretograms after PCR (Polymerase Chain Reaction) amplification respectively of 11 goose variety (system) DNA by three genetic labels are formed for identifying whether the identified goose groups are true Szechwan white geese. According to the invention, the technical blank for labeling and identifying outstanding local goose variety of Szechwan white geese by utilizing molecular labeling is filled, and a reliable, rapid and accurate molecular biology method is provided for identifying the Szechwan white geese; unreliable factors for identifying Szechwan white geese by utilizing a traditional morphological identification method are eliminated; and technical guarantee of wide application in production of true high-fecundity Szechwan white geese can be provided.

Description

technical field [0001] The patent belongs to the technical field of identification of genetic resources of livestock and poultry, and specifically relates to a method for identifying Sichuan white goose from other goose varieties by using molecular markers. Background technique [0002] As a kind of herbivorous poultry, geese are popular in the market, and the goose industry has developed rapidly in my country. In recent years, the annual breeding and slaughtering of geese in my country accounted for more than 90% of the world's total. Sichuan white goose is one of the excellent genetic resources among meat goose breeds in my country. It has excellent characteristics such as resistance to rough feeding, strong disease resistance, early sexual maturity, high egg production, fast growth, and delicious meat. Due to its high reproductive rate (annual egg production of about 80 eggs, much higher than the 30-50 eggs of other meat goose breeds at home and abroad), basically no nest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 李亮何桦刘贺贺韩春春王继文孙文强汤静思
Owner SICHUAN AGRI UNIV
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